Dynamic Regulation of ARGONAUTE4 within Multiple Nuclear Bodies in Arabidopsis thaliana

DNA methylation directed by 24-nucleotide small RNAs involves the small RNA-binding protein ARGONAUTE4 (AGO4), and it was previously shown that AGO4 localizes to nucleolus-adjacent Cajal bodies, sites of snRNP complex maturation. Here we demonstrate that AGO4 also localizes to a second class of nuclear bodies, called AB-bodies, which are found immediately adjacent to condensed 45S ribosomal DNA (rDNA) sequences. AB-bodies also contain other proteins involved in RNA-directed DNA methylation including NRPD1b (a subunit of the RNA Polymerase IV complex, RNA PolIV), NRPD2 (a second subunit of this complex), and the DNA methyltransferase DRM2. These two classes of AGO4 bodies are structurally independent—disruption of one class does not affect the other—suggesting a dynamic regulation of AGO4 within two distinct nuclear compartments in Arabidopsis. Abolishing Cajal body formation in a coilin mutant reduced overall AGO4 protein levels, and coilin dicer-like3 double mutants showed a small decrease in DNA methylation beyond that seen in dicer-like3 single mutants, suggesting that Cajal bodies are required for a fully functioning DNA methylation system in Arabidopsis

The Modelling of InfraRed Dark Clouds

This paper presents results from modelling 450 micron and 850 micron continuum and HCO+ line observations of three distinct cores of an infrared dark cloud (IRDC) directed toward the W51 GMC. In the sub-mm continuum these cores appear as bright, isolated emission features. One of them coincides with the peak of 8.3 micron extinction as measured by the Midcourse Space Experiment satellite. Detailed radiative transfer codes are applied to constrain the cores' physical conditions to address the key question: Do these IRDC-cores harbour luminous sources? The results of the continuum model, expressed in the $\chi^2$ quality-of-fit parameter, are also constrained by the absence of 100 micron emission from IRAS. For the sub-mm emission peaks this shows that sources of 300 solar luminosities are embedded within the cores. For the extinction peak, the combination of continuum and HCO+ line modelling indicates that a heating source is present as well. Furthermore, the line model provides constraints on the clumpiness of the medium. All three cores have similar masses of about 70-150 solar masses and similar density structures. The extinction peak differs from the other two cores by hosting a much weaker heating source, and the sub-mm emission core at the edge of the IRDC deviates from the other cores by a higher internal clumpiness.Comment: 13 pages, 13 figures, accepted for publication in A&

Ion-pairing chromatography and amine derivatization provide complementary approaches for the targeted LC-MS analysis of the polar metabolome.

Liquid chromatography coupled to mass spectrometry is a key metabolomics/metabonomics technology. Reversed-phase liquid chromatography (RPLC) is very widely used as a separation step, but typically has poor retention of highly polar metabolites. Here, we evaluated the combination of two alternative methods for improving retention of polar metabolites based on 6-aminoquinoloyl-N-hydroxysuccinidimyl carbamate derivatization for amine groups, and ion-pairing chromatography (IPC) using tributylamine as an ion-pairing agent to retain acids. We compared both of these methods to RPLC and also to each other, for targeted analysis using a triple-quadrupole mass spectrometer, applied to a library of ca. 500 polar metabolites. IPC and derivatization were complementary in terms of their coverage: combined, they improved the proportion of metabolites with good retention to 91%, compared to just 39% for RPLC alone. The combined method was assessed by analyzing a set of liver extracts from aged male and female mice that had been treated with the polyphenol compound ampelopsin. Not only were a number of significantly changed metabolites detected, but also it could be shown that there was a clear interaction between ampelopsin treatment and sex, in that the direction of metabolite change was opposite for males and females

Graphene Saturable Absorbers at Terahertz Frequency from Liquid Phase Exfoliation of Graphite

© 2018 OSA. We report on the development of terahertz (THz) saturable-absorbers exploiting printable graphene inks. The achieved 80% transparency modulation at 3.5 THz makes these devices potential candidates as passive components for THz solid-state lasers.EPSR

Initial phases of massive star formation in high infrared extinction clouds. I. Physical parameters

The earliest phases of massive star formation are found in cold and dense infrared dark clouds (IRDCs). Since the detection method of IRDCs is very sensitive to the local properties of the background emission, we present here an alternative method to search for high column density in the Galactic plane by using infrared extinction maps. We find clouds between 1 and 5 kpc, of which many were missed by previous surveys. By studying the physical conditions of a subsample of these clouds, we aim at a better understanding of the initial conditions of massive star formation. We made extinction maps of the Galactic plane based on the 3.6-4.5 microns color excess between the two shortest wavelength Spitzer IRAC bands, reaching to visual extinctions of ~100 mag and column densities of 9x10^22 cm^-2. From this we compiled a new sample of cold and compact high extinction clouds. We used the MAMBO array at the IRAM 30m telescope to study the morphology, masses and densities of the clouds and the dense clumps within them. The latter were followed up by pointed ammonia observations with the 100m Effelsberg telescope, to determine rotational temperatures and kinematic distances. Extinction maps of the Galactic plane trace large scale structures such as the spiral arms. The 1.2 mm emission maps reveal that the high extinction clouds contain extended cold dust emission, from filamentary structures to still diffuse clouds. Most of the clouds are dark in 24 microns, but several show already signs of star formation via maser emission or bright infrared sources, suggesting that the high extinction clouds contain a variety of evolutionary stages. The observations suggest an evolutionary scheme from dark, cold and diffuse clouds, to clouds with a stronger 1.2 mm peak and to finally clouds with many strong 1.2 mm peaks, which are also warmer, more turbulent and already have some star formation signposts.Comment: 26 pages, 14 figures, 9 tables. Accepted for publication in Astronomy and Astrophysics, small corrections implemente

Star Formation in the Milky Way. The Infrared View

I present a brief review of some of the most recent and active topics of star formation process in the Milky Way using mid and far infrared observations, and motivated by the research being carried out by our science group using data gathered by the Spitzer and Herschel space telescopes. These topics include bringing together the scaling relationships found in extragalactic systems with that of the local nearby molecular clouds, the synthetic modeling of the Milky Way and estimates of its star formation rate.Comment: 12 pages, 9 figures. To apper in "Cosmic-ray induced phenomenology in star-forming environments: Proceedings of the 2nd Session of the Sant Cugat Forum of Astrophysics" (April 16-19, 2012), Olaf Reimer and Diego F. Torres (eds.

Terahertz Frequency Combs Exploiting an On-Chip, Solution-Processed, Graphene-Quantum Cascade Laser Coupled-Cavity.

The ability to engineer quantum-cascade-lasers (QCLs) with ultrabroad gain spectra, and with a full compensation of the group velocity dispersion, at terahertz (THz) frequencies, is key for devising monolithic and miniaturized optical frequency-comb-synthesizers (FCSs) in the far-infrared. In THz QCLs four-wave mixing, driven by intrinsic third-order susceptibility of the intersubband gain medium, self-locks the optical modes in phase, allowing stable comb operation, albeit over a restricted dynamic range (∼20% of the laser operational range). Here, we engineer miniaturized THz FCSs, comprising a heterogeneous THz QCL, integrated with a tightly coupled, on-chip, solution-processed, graphene saturable-absorber reflector that preserves phase-coherence between lasing modes, even when four-wave mixing no longer provides dispersion compensation. This enables a high-power (8 mW) FCS with over 90 optical modes, through 55% of the laser operational range. We also achieve stable injection-locking, paving the way to a number of key applications, including high-precision tunable broadband-spectroscopy and quantum-metrology