108 research outputs found

    Silicate electrochemical measurements in seawater: chemical and analytical aspects towards a reagentless sensor

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    From the study of molybdenum oxidation in aqueous solutions we developed a semi-autonomous method to detect silicate in aqueous samples. Molybdenum oxidation was used to form molybdate in acidic media. The silicomolybdic complex formed with silicate is detectable by amperometry or cyclic voltammetry. The new electrochemical method is in good agreement with the method conventionally used for environmental water silicate analysis. In the second stage, a completely reagentless method was developed using molybdate and proton produced during molybdenum oxidation. Reproducibility tests show a precision of 2.6% for a concentration of 100 μmol L−1. This new method will be very suitable for the development of new autonomous silicate sensors easy to handle and without reagents. In this paper we present the analytical and chemical aspects necessary for a complete documentation of the method before the development of a new reagentless sensor

    Soluble, Colloidal, and Particulate Iron Across the Hydrothermal Vent Mixing Zones in Broken Spur and Rainbow, Mid-Atlantic Ridge

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    The slow-spreading Mid-Atlantic Ridge (MAR) forms geological heterogeneity throughout the ridge system by deep crustal faults and their resultant tectonic valleys, which results in the existence of different types of hydrothermal vent fields. Therefore, investigating MAR hydrothermal systems opens a gate to understanding the concentration ranges of ecosystem-limiting metals emanating from compositionally distinct fluids for both near-field chemosynthetic ecosystems and far-field transport into the ocean interiors. Here, we present novel data regarding onboard measured, size-fractionated soluble, colloidal, and particulate iron concentrations from the 2018 R/V L'Atalante - ROV Victor research expedition, during which samples were taken from the mixing zone of black smokers using a ROV-assisted plume sampling. Iron size fractionation (200nm) data were obtained from onboard sequential filtering, followed by measurement via ferrozine assay and spectrophotometric detection at 562nm. Our results showed the persistent presence of a nanoparticulate/colloidal phase (retained within 20-200nm filtrates) even in high-temperature samples. A significant fraction of this phase was retrievable only under treatment with HNO3 - a strong acid known to attack and dissolve pyrite nanocrystals. Upon mixing with colder bottom waters and removal of iron in the higher parts of the buoyant plume, the larger size fractions became dominant as the total iron levels decreased, but it was still possible to detect significant (micromolar) levels of nanoparticulate Fe even in samples collected 5m above the orifice in the rising plume. The coolest sample

    Interactions of Deep-Sea Vent Invertebrates with Their Environment: The Case of Rimicaris exoculata

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    The vent shrimp Rimicaris exoculata thrives around many hydrothermal vent sites along the Mid-Atlantic Ridge (MAR), where it aggregates into dense swarms. In contrast to hydrothermal vent fields at the East Pacific Rise (EPR), where the biomass is dominated by tubeworms, clams, and mussels, this shrimp is one of the major animal species at MAR vents. These animals are found in the dynamic mixing interface between cold oxygenated seawater and hot, reduced hydrothermal vent fluid. The adaptation of this shrimp to the hostile deep-sea hydrothermal environment and its survival strategy has been investigated since their discovery at the TAG site in the late 1980s. Rimicaris exoculata is now known to colonize black smoker complexes along the MAR in the depth-range of 2,300-3,900 in (Rainbow, Broken Spur, TAG, Snake Pit, Logatchev, 5 degrees S (Rimicaris of exoculata). Although the presence of the Rimicaris genus was first believed to be restricted to the MAR, a related species, Rimicaris kairei, was found recently at the Central Indian Ridge (CIA) (Edmonds and Kairei vent field). This review summarizes the current knowledge of Rimicaris shrimp, focusing on their spatial and temporal distribution, chemical and thermal environment, as well as on possible nutrition strategies and behavioral aspects. Recent studies suggested that iron oxide encrusted bacteria hosted in the branchial chamber of R. exoculata from the Rainbow vent field (MAR) might rely on iron oxidation. Striking results on the occurrence and morphology of iron precipitates, as well as on bacterial-mineral interaction in the gill chamber, have lead to the hypothesis of an iron-based symbiosis between bacteria and the shrimp. Special attention is called to these issues

    Silicate determination in sea water: toward a reagentless electrochemical method

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    ilicate has been determined in sea water by four different electrochemical methods based on the detection of the silicomolybdic complex formed in acidic media by the reaction between silicate and molybdenum salts. The first two methods are based on the addition of molybdate and protons in a seawater sample in an electrochemical cell. Cyclic voltammetry presents two reduction and two oxidation peaks giving four values of the concentration and therefore increasing the precision. Then chronoamperometry is performed on an electrode held at a constant potential. A semi-autonomous method has been developed based on the electrochemical anodic oxidation of molybdenum, the complexation of the oxidation product with silicate and the detection of the complex by cyclic voltammetry. This method is tested and compared with the classical colorimetric one during ANT XXIII/3 cruise across Drake Passage (January–February 2006). The detection limit is 1 μM and the deviation between both methods is less than 3% for concentrations higher than 10 μM. Finally a complete reagentless method with a precision of 2.6% is described based on the simultaneous formation of the molybdenum salt and protons in a divided electrochemical cell. This latter method should be very useful for developing a reagentless sensor suitable for long term in situ deployments on oceanic biogeochemical observatories

    Animal community dynamics at senescent and active vents at the 9° N East Pacific Rise after a volcanic eruption

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    © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Gollner, S., Govenar, B., Arbizu, P. M., Mullineaux, L. S., Mills, S., Le Bris, N., Weinbauer, M., Shank, T. M., & Bright, M. Animal community dynamics at senescent and active vents at the 9° N East Pacific Rise after a volcanic eruption. Frontiers in Marine Science, 6, (2020): 832, doi:10.3389/fmars.2019.00832.In 2005/2006, a major volcanic eruption buried faunal communities over a large area of the 9°N East Pacific Rise (EPR) vent field. In late 2006, we initiated colonization studies at several types of post eruption vent communities including those that either survived the eruption, re-established after the eruption, or arisen at new sites. Some of these vents were active whereas others appeared senescent. Although the spatial scale of non-paved (surviving) vent communities was small (several m2 compared to several km2 of total paved area), the remnant individuals at surviving active and senescent vent sites may be important for recolonization. A total of 46 meio- and macrofauna species were encountered at non-paved areas with 33 of those species detected were also present at new sites in 2006. The animals living at non-paved areas represent refuge populations that could act as source populations for new vent sites directly after disturbance. Remnants may be especially important for the meiofauna, where many taxa have limited or no larval dispersal. Meiofauna may reach new vent sites predominantly via migration from local refuge areas, where a reproductive and abundant meiofauna is thriving. These findings are important to consider in any potential future deep-sea mining scenario at deep-sea hydrothermal vents. Within our 4-year study period, we regularly observed vent habitats with tubeworm assemblages that became senescent and died, as vent fluid emissions locally stopped at patches within active vent sites. Senescent vents harbored a species rich mix of typical vent species as well as rare yet undescribed species. The senescent vents contributed significantly to diversity at the 9°N EPR with 55 macrofaunal species (11 singletons) and 74 meiofaunal species (19 singletons). Of these 129 species associated with senescent vents, 60 have not been reported from active vents. Tubeworms and other vent megafauna not only act as foundation species when alive but provide habitat also when dead, sustaining abundant and diverse small sized fauna.We received funding from the Austrian FWF (GrantP20190-B17; MB), the U.S. National Science Foundation (OCE-0424953; to LM, D. McGillicuddy, A. Thurnherr, J. Ledwell, and W. Lavelle; and OCE-1356738 to LM), and the European Union Seventh Framework Programme (FP7/2007-2013) under the MIDAS project, Grant Agreement No. 603418. Ifremer and CNRS (France) supported NL cruise participation and sensor developments. BG was supported by a postdoctoral fellowship from the Deep Ocean Exploration Institute at WHOI (United States). TS was supported by the U.S. National Science Foundation (OCE-0327261 to TS and OCE-0937395 to TS and BG)

    Dynamic drivers of a shallow-water hydrothermal vent ecogeochemical system (Milos, Eastern Mediterranean)

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    Shallow-water hydrothermal vents share many characteristics with their deep-sea analogs. However, despite ease of access, much less is known about the dynamics of these systems. Here, we report on the spatial and temporal chemical variability of a shallow-water vent system at Paleochori Bay, Milos Island, Greece, and on the bacterial and archaeal diversity of associated sandy sediments. Our multi-analyte voltammetric profiles of dissolved O2 and hydrothermal tracers (e.g. Fe2+, FeSaq, Mn2+) on sediment cores taken along a transect in hydrothermally affected sediments indicate three different areas: the central vent area (highest temperature) with a deeper penetration of oxygen into the sediment, and a lack of dissolved Fe2+ and Mn2+; a middle area (0.5 m away) rich in dissolved Fe2+ and Mn2+ (exceeding 2 mM) and high free sulfide with potential for microbial sulfide oxidation as suggested by the presence of white mats at the sediment surface; and, finally, an outer rim area (1-1.5 m away) with lower concentrations of Fe2+ and Mn2+ and higher signals of FeSaq, indicating an aged hydrothermal fluid contribution. In addition, high-frequency temperature series and continuous in situ H2S measurements with voltammetric sensors over a 6-day time period at a distance 0.5 m away from the vent center showed substantial temporal variability in temperature (32 to 46 ºC ) and total sulfide (488 to 1329 �M) in the upper sediment layer. Analysis of these data suggests that tides, winds, and abrupt geodynamic events generate intermittent mixing conditions lasting for several hours to days. Despite substantial variability, the concentration of sulfide available for chemoautotrophic microbes remained high. These findings are consistent with the predominance of Epsilonproteobacteria in the hydrothermally influenced sediments Diversity and metagenomic analyses on sediments and biofilm collected along a transect from the center to the outer rim of the vent provide further insights on the metabolic activities and the environmental factors shaping these microbial communities. Both bacterial and archaeal diversity changed along the transect as well as with sediment depth, in line with the geochemical measurements. Beside the fact that it harbors an unexpected diversity of yet undescribed bacteria and archaea, this site is also a relevant model to investigate the link between ecological and abiotic dynamics in such instable hydrothermal environments. Our results provide evidence for the importance of transient geodynamic and hydrodynamic events in the dynamics and distribution of chemoautotrophic communities in the hydrothermally influenced sediments of Paleochori Bay

    Hypotaurine and thiotaurine as indicators of sulfide exposure in bivalves and vestimentiferans from hydrothermal vents and cold seeps

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    Author Posting. © Blackwell, 2007. This is the author's version of the work. It is posted here by permission of Blackwell for personal use, not for redistribution. The definitive version was published in Marine Ecology 28 (2007): 208-218, doi:10.1111/j.1439-0485.2006.00113.x.Vesicomyid clams, vestimentiferans, and some bathymodiolin mussels from hydrothermal vents and cold seeps possess thiotrophic endosymbionts, high levels of hypotaurine and, in tissues with symbionts, thiotaurine. The latter, a product of hypotaurine and sulfide, may store and/or transport sulfide non-toxically, and the ratio to hypotaurine plus thiotaurine (Th/[H+Th]) may reflect an animal's sulfide exposure. To test this, we analyzed seep and vent animals with in situ sulfide measurements. Calyptogena kilmeri clams occur at high-sulfide seeps in Monterey Canyon, while C. (Vesicomya) pacifica clams occur at seeps with lower levels but take up and metabolize sulfide more effectively. From one seep where they co-occur, both had gill thiotaurine contents at 22-25 mmol/kg wet mass, and while C. (V.) pacifica had a higher blood sulfide level, it had a lower Th/[H+Th] (0.39) than C. kilmeri (0.63). However, these same species from different seeps with lower sulfide exposures had lower ratios. Bathymodiolus thermophilus (East Pacific Rise [EPR 9°50'N]) from high- (84 μM) and a low- (7 μM) sulfide vents had gill ratios of 0.40 and 0.12, respectively. Trophosomes of Riftia pachyptila (EPR 9°50'N) from medium- (33 μM) and low- (4 μM) sulfide vents had ratios of 0.23 and 0.20, respectively (not significantly different). Ridgeia piscesae vestimentiferans (Juan de Fuca Ridge) have very different phenotypes at high- and low-sulfide sites, and their trophosomes had the greatest differences: 0.81 and 0.04 ratios from high- and low-sulfide sites, respectively. Thus Th/[H+Th] may indicate sulfide exposure levels within species, but not in interspecies comparisons, possibly due to phylogenetic and metabolic differences. Total H+Th was constant within each species (except in R. piscesae); the sum may indicate the maximum potential sulfide load that a species faces.Funding for Ridgeia piscesae collection was grant UAF01-0042 from NOAA-West Coast National Undersea Research Center to Stephen W. Schaeffer, Charles R. Fisher, and Stéphane Hourdez. Funding for GLB, RVH and PHY was the W.M. Keck Foundation (grant to Whitman College Life Sciences program) and Whitman College Perry Grant program. Funding was from Ifremer for NLB, and The David and Lucile Packard Foundation for SKG

    Dissolved and particulate metals (Fe, Zn, Cu, Cd, Pb) in two habitats from an active hydrothermal field on the EPR at 13°N

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    Le texte intégral est accessible via Archimer: http://archimer.ifremer.fr/doc/2008/publication-3830.pdfInternational audienceThe distribution of Fe, Cu, Zn, Pb, Cd between the dissolved ( 2 μm) fractions was measured after in-situ filtration in two hydrothermal habitats. The total metal concentration ranges exhibit a clear enrichment compared with the seawater concentration, accounting for the hydrothermal input for all the metals considered. Iron is the predominant metal (5-50 μM) followed by Zn and Cu. Cd and Pb are present at the nM level. At the scale studied, the behavior of temperature, pH and dissolved iron is semi-conservative whereas the other dissolved and particulate metals are characterized by non-conservative patterns. The metal enrichment of the > 2 μm fraction results from the settlement and accumulation of particulate matter close to the organisms, acting as a secondary metal source. The enrichment observed in the dissolved fraction can be related to the dissolution or oxidation of particles (mainly polymetallic sulfide) or to the presence of small particles and large colloids not retained on the 2 μm frit. SEM observations indicate that the bulk particulate observed is characteristic of crystalline particles settling rapidly from the high temperature smoker (sphalerite, wurtzite and pyrite), amorphous structures and eroded particles formed in the external zone of the chimney. Precipitation of Zn, Cu, Cd and Pb with Fe as wurtzite, sphalerite and pyrite is the main process taking place within the area studied and is semi-quantitative. The distribution of the dominant observed fauna has been related to the gradient resulting from the dilution process, with the alvinellids worms colonizing the hotter and more variable part of the mixing zone, but also to the metallic load of the mixing zone. Dissolved and particulate metal concentrations are therefore necessary abiotic factors to be studied in a multiparametric approach to understand the faunal distribution in hydrothermal ecosystems

    Hydrothermal Energy Transfer and Organic Carbon Production at the Deep Seafloor

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    In just four decades, hundreds of hydrothermal vent fields have been discovered, widely distributed along tectonic plate boundaries on the ocean floor. Vent invertebrate biomass reaching up to tens of kilograms per square meter has attracted attention as a potential contributor to the organic carbon pool available in the resource-limited deep sea. But the rate of chemosynthetic production of organic carbon at deep-sea hydrothermal vents is highly variable and still poorly constrained. Despite the advent of molecular techniques and in situ sensing technologies, the factors that control the capacity of vent communities to exploit the available chemical energy resources remain largely unknown. Here, we review key drivers of hydrothermal ecosystem productivity, including (a) the diverse mechanisms governing energy transfer among biotic and abiotic processes; (b) the tight linkages among these processes; and (c) the nature and extent of spatial and temporal diversity within a variety of geological settings; and (d) the influence of these and other factors on the turnover of microbial primary producers, including those associated with megafauna. This review proposes a revised consideration of the pathways leading to the biological conversion of inorganic energy sources into biomass in different hydrothermal habitats on the seafloor. We propose a conceptual model that departs from the canonical conservative mixing-continuum paradigm by distinguishing low-temperature diffuse flows (LT-diffuse flows) derived from seawater and high-temperature fluids (HT-diffuse flow) derived from end-member fluids. We further discuss the potential for sustained organic matter production at vent-field scale, accounting for the natural instability of hydrothermal ecosystems, from the climax vent communities of exceptional productivity to the long-term lower-activity assemblages. The parameterization of such a model crucially needs assessment of in situ rates and of the largely unrecognized natural variability on relevant temporal scales. Beyond the diversity of hydrothermal settings, the depth range and water mass distribution over oceanic ridge crests, volcanic arcs and back-arc systems are expected to significantly influence biomass production rates. A particular challenge is to develop observing strategies that will account for the full range of environmental variables while attempting to derive global or regional estimates

    Detecting the influence of initial pioneers on succession at deep-sea vents

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    © The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 7 (2012): e50015, doi:10.1371/journal.pone.0050015.Deep-sea hydrothermal vents are subject to major disturbances that alter the physical and chemical environment and eradicate the resident faunal communities. Vent fields are isolated by uninhabitable deep seafloor, so recolonization via dispersal of planktonic larvae is critical for persistence of populations. We monitored colonization near 9°50′N on the East Pacific Rise following a catastrophic eruption in order to address questions of the relative contributions of pioneer colonists and environmental change to variation in species composition, and the role of pioneers at the disturbed site in altering community structure elsewhere in the region. Pioneer colonists included two gastropod species: Ctenopelta porifera, which was new to the vent field, and Lepetodrilus tevnianus, which had been rare before the eruption but persisted in high abundance afterward, delaying and possibly out-competing the ubiquitous pre-eruption congener L. elevatus. A decrease in abundance of C. porifera over time, and the arrival of later species, corresponded to a decrease in vent fluid flow and in the sulfide to temperature ratio. For some species these successional changes were likely due to habitat requirements, but other species persisted (L. tevnianus) or arrived (L. elevatus) in patterns unrelated to their habitat preferences. After two years, disturbed communities had started to resemble pre-eruption ones, but were lower in diversity. When compared to a prior (1991) eruption, the succession of foundation species (tubeworms and mussels) appeared to be delayed, even though habitat chemistry became similar to the pre-eruption state more quickly. Surprisingly, a nearby community that had not been disturbed by the eruption was invaded by the pioneers, possibly after they became established in the disturbed vents. These results indicate that the post-eruption arrival of species from remote locales had a strong and persistent effect on communities at both disturbed and undisturbed vents.The authors received funding from National Science Foundation grant OCE-0424953, WHOI Deep Ocean Exploration Institute, WHOI Summer Student Fellow program, Woods Hole Partnership in Education Program, IFREMER and CNRS, Fondation TOTAL Chair Extreme Marine Environment, Biodiversity and Global change
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