Sensing and Tactile Artificial Muscles from Reactive Materials

Films of conducting polymers can be oxidized and reduced in a reversible way. Any intermediate oxidation state determines an electrochemical equilibrium. Chemical or physical variables acting on the film may modify the equilibrium potential, so that the film acts as a sensor of the variable. The working potential of polypyrrole/DBSA (Dodecylbenzenesulfonic acid) films, oxidized or reduced under constant currents, changes as a function of the working conditions: electrolyte concentration, temperature or mechanical stress. During oxidation, the reactive material is a sensor of the ambient, the consumed electrical energy being the sensing magnitude. Devices based on any of the electrochemical properties of conducting polymers must act simultaneously as sensors of the working conditions. Artificial muscles, as electrochemical actuators constituted by reactive materials, respond to the ambient conditions during actuation. In this way, they can be used as actuators, sensing the surrounding conditions during actuation. Actuating and sensing signals are simultaneously included by the same two connecting wires

SURVIVAL CAPACITY OF Arcobacter butzleri INOCULATED IN POULTRY MEAT AT TWO DIFFERENT REFRIGERATION TEMPERATURES

Arcobacter spp. are emerging enteropathogens and potential zoonotic agents that can be transmitted by food and water, being considered a public health risk. The high isolation rate of these bacteria from poultry products suggests that it may be a major source of human infections. One hallmark for differentiating the genus Arcobacter fromCampylobacter includes their growing capacity at low temperatures (15-30 °C) under aerobic conditions. However, little is known about the population density variation of these bacteria at different refrigeration temperatures. The aim of this study was to determine the survival behavior of two different Arcobacter butzleri concentrations (104 CFU/mL and 107 CFU/mL) inoculated on chicken legs and held at two different refrigeration temperatures (4 and 10 °C) throughout storage time. Results have shown that A. butzleri had growing capacity both at 4 and 10 °C. No statistical difference between the survival trends was found for both bacterial concentrations and temperatures tested. This study shows that A. butzleri is a robust species with regard to storage temperature, and represents a potential health risk for poultry meat consumers

First isolation report of Arcobacter cryaerophilus from a human diarrhea sample in Costa Rica

Arcobacter cryaerophilus is an emerging enteropathogen and potential zoonotic agent transmitted by food and water. In Costa Rica, this bacterium has not been associated with cases of human gastroenteritis, even though it has been isolated from farm animals, especially poultry. This paper reports the first isolation of A. cryaerophilus from a human case of bloody watery diarrhea and the virulence genes associated with this isolate

Tiempo de crecimiento bacteriano en hemocultivos en neonatos

ResumenIntroducciónLa sepsis es causa importante de morbimortalidad neonatal.ObjetivosDetectar el tiempo en que la curva de crecimiento bacteriano es evidenciada en la muestra de sangre inoculada en los hemocultivos y comparar estos tiempos de crecimiento bacteriano entre bacterias gramnegativas y grampositivas, entre los tipos de sepsis neonatal y determinar las bacterias más frecuentemente aisladas entre neonatos prematuros y de término.Pacientes y métodoEstudio descriptivo de recién nacidos en riesgo de sepsis o con sospecha de sepsis por manifestaciones clínicas o de laboratorio, en que se evaluaron 114 hemocultivos positivos entre 1.932 hemocultivos tomados entre mayo de 2010 y mayo de 2014. Los datos se analizaron con Stata® 11.0.ResultadosEl 5,9% de los hemocultivos tuvieron crecimiento bacteriano. La mediana y rango intercuartílico de tiempos de crecimiento bacteriano para gramnegativos fue 11h (10-13h), para grampositivos diferentes a Staphylococcus coagulasa negativo (SCoN) 12h (12-18h) y para SCoN 42h (36-44h). El 95,8% de las bacterias grampositivas y el 96% de las gramnegativas tuvieron tiempos de crecimiento bacteriano≤24h de incubación, mientras que en los SCoN el 100% de los hemocultivos fue positivo en≤62h de incubación.ConclusiónEl 100% de sepsis por bacterias gramnegativas, grampositivas no SCoN y 90% de las ocasionadas por SCoN, son identificadas en los hemocultivos en las primeras 48h, por lo cual podemos concluir que para descartar una sepsis, un período de incubación en hemocultivos de 48h es suficiente.AbstractIntroductionSepsis is a major cause of neonatal morbidity and mortality.ObjectivesTo detect the time when the bacterial growth curve is evidenced in the blood sample inoculated blood cultures and comparing the times of bacterial growth between Gram negative and Gram positive bacteria, among the types of neonatal sepsis and identifying microorganisms more often isolated from preterm and term.Patients and methodA descriptive study. 114 positive blood cultures from 1,932 blood cultures taken from 01-May-2010 and 31-May-2014 were evaluated. Data were analyzed with Stata® 11.0.Results5.9% of blood cultures had bacterial growth. The median and interquartile range of Gram negative times of bacterial growth was 11h (10-13h), for Gram positive coagulase-negative Staphylococcus different (CoNS) 12h (12-18h) and CoNS 42h (36-44h). 95.8% of Gram positive and 96% of Gram negative, were the times of bacterial growth≤24h incubation, whereas the 100% CoNS was positive≤62h of incubation.Conclusion100% of sepsis by Gram negative and Gram positive no CoNS and 90% of those caused by CoNS are identified in blood cultures in 48h, so we can conclude that to rule out sepsis, an incubation period of 48h in blood cultures is sufficient

Core Indicators to Assess Quality of Life in Population with Brain Injury

Abstract Assessing quality of life of people with brain injury has a relevant role for developing strategies focused on personal outcomes that allow us to guide good practices and rehabilitation. So far, assessment of quality of life for this population has been restricted to an evaluation of personal outcomes from a health-related quality of life perspective. This approach it is mostly centered on physical health, however, quality of life needs to be addressed from a holistic and multidimensional perspective. The goal of this study is to identify core indicators of quality of life in brain injury based on a comprehensive theoretical model focused on the most relevant aspects of this population functioning. A Delphi study was carried out to obtain the specific core indicators of quality of life for this population. The methodology used to reach a consensus about the best indicators and items to measure quality of life involved four rounds and 14 experts on rehabilitation of people with brain injury. The Delphi study provided evidence of content validity for the field-test version of a new scale that will be applied to a wide sample in order to empirically check its suitability for this population

The Toxicity Exerted by the Antibiotic Sulfadiazine on the Growth of Soil Bacterial Communities May Increase over Time

The toxicity exerted by the antibiotic sulfadiazine on the growth of soil bacterial communities was studied in two agricultural soils for a period of 100 days. In the short-term (2 days of incubation), the effect of sulfadiazine on bacterial growth was low (no inhibition or inhibition <32% for a dose of 2000 mg·kg−1). However, sulfadiazine toxicity increased with time, achieving values of 40% inhibition, affecting bacterial growth in both soils after 100 days of incubation. These results, which were here observed for the first time for any antibiotic in soil samples, suggest that long-term experiments would be required for performing an adequate antibiotics risk assessment, as short-term experiments may underestimate toxicity effectsThis study has been funded by the Spanish Ministry of Economy and Competitiveness through the projects CGL2015-67333-C2-1-R and -2-R (FEDER Funds), and by Xunta de Galicia via CITACA Strategic Partnership (ED431E 2018/07) and BV1 research group (ED431C 2017/62-GRC). David Fernández Calviño holds a Ramón y Cajal contract (RYC-2016-20411) financed by the Spanish Ministry of Economy Industry and Competitiveness. Vanesa Santás Miguel holds a pre-doctoral fellowship founded by the University of VigoS

Food addiction and its relationship with other eating behaviours among Spanish university students

BackgroundFood addiction (FA) is characterised by symptoms such as loss of control over food consumption, inability to reduce consumption despite the desire to do so, and continued consumption despite negative consequences. The modified Yale Food Addiction Scale 2.0 (mYFAS 2.0) is a widely used instrument to assess FA.ObjectivesTo validate the Spanish mYFAS 2.0; to analyse the relationships between FA with other eating behaviours, sociodemographic variables, and Body Mass Index (BMI); and to test the eating-related variables that account for the variance in FA.MethodsThe sample consisted of 400 university students (M-age = 24.16, SDage = 6.12; 51% female), who completed the mYFAS 2.0 and measures of eating-related constructs.ResultsA confirmatory factor analysis (CFA) supported the one-factor structure of the mYFAS 2.0. The scale showed good internal consistency (alpha = .78), and good convergent validity with the mYFAS. FA was related to eating styles, binge eating, and bulimia. No differences in FA were observed between males and females, and there was no association between FA and BMI. In addition, younger participants scored higher on FA than older participants. The eating-related variables explain 54.7% of the variance in FA.ConclusionsThe mYFAS 2.0 is a valid and reliable scale to assess FA in the Spanish population. The positive and significant relationship of variables related to eating (eating styles, binge eating and bulimia) with FA was demonstrated. These variables were indicated by those at high risk of FA. Plain English summaryFood addiction (FA) is characterised by excessive and dysregulated intake of high-calorie foods. Loss of control over food consumption, inability to reduce consumption, and continued consumption despite negative consequences are some of its symptoms. The modified Yale Food Addiction Scale 2.0 (mYFAS 2.0) is a brief instrument specifically developed to assess FA. The objectives of this study were: (1) to examine the psychometric properties of the Spanish mYFAS 2.0; (2) to analyse the relationships between FA with other eating behaviours, sociodemographic variables, and Body Mass Index (BMI); and (3) to test whether there are certain dietary variables that may be positively related to FA. We were able to provide evidence that the mYFAS 2.0 is a valid and reliable scale for assessing FA in the Spanish population. The positive and significant relationship of eating-related variables (eating styles, binge eating and bulimia) with FA was demonstrated. These variables can be considered to identify subgroups at high risk of FA

Clarithromycin Effect in Microbial Communities

Financiaciado para publicación en acceso aberto: Universidade de Vigo/CISUGA laboratory experiment was carried out to investigate the response of the microbial communities in acid agricultural soils located in the NW Iberian Peninsula to the presence of clarithromycin. Four soils, with different organic C content and similar pH, and seven different concentrations of clarithromycin (0.49, 1.95, 7.81, 31.25, 125, 500 and 2,000 mg kg−1 of soil) were used, and microbial estimates were made after 8 and 42 incubation days. The phospholipid fatty acids (PLFA) technique was used to estimate the total microbial biomass and biomass of specific microbial groups as well as the microbial community structure (PLFA pattern). The microbial biomass (total and specific groups) was different in the four studied soils, the lowest values being exhibited by soils with the lowest organic C. The antibiotic addition showed a positive effect on microbial biomass (total and specific groups), especially at the highest dose; the effect being similar or even more accentuated with time passed after the addition (42 days ≥8 days). Principal component analysis (PCA) of the PLFA data carried out with the whole data set showed that the main determining factors of the microbial structure followed the order: soil > time incubation ≥ antibiotic dose. When the PCA was performed individually for each incubation time, the results indicated that microbial communities of the four soils were different. Likewise, for each soil, different microbial communities were observed depending on antibiotic concentration. The microbial biomass and PLFA pattern data were coincidentally showing that the clarithromycin addition favored fungi and G− bacteria more that bacteria and G+ bacteria; the effect being dose-dependent. Our data (microbial biomass, PLFA pattern) also demonstrated that the effect of clarithromycin addition on microbial communities in these four acid agricultural soils persisted even after 42 incubation days.Xunta de Galicia | Ref. ED481B-2022-081Ministerio de Ciencia, Innovación y Universidades | Ref. RTI2018-099574-B-C21Ministerio de Ciencia, Innovación y Universidades | Ref. RTI2018-099574-B-C22Ministerio de Ciencia, Innovación y Universidades | Ref. FPU21/0420

Extracción de un cuerpo extraño de la arteria descendente anterior: un caso clínico.

[ES] En el presente trabajo presentamos el caso de un paciente de 89 años, sometido a un cateterismo cardiaco en el que se observa una importante calcificación coronaria severa en todo el territorio de la descendente anterior. Valorando la situación clínica del paciente, y tras la discusión en equipo multidisciplinar, se decide intervencionismo percutáneo para tratar dicha lesión en el trascurso del cual ocurre una complicación poco habitual como es la rotura del tubo del balón, quedando atrapado en el interior de dicha arteria. Este evento inesperado obliga a proceder a la extracción de dicho «cuerpo extraño», utilizando diferentes técnicas, empleando finalmente la técnica de doble guía más trapping, con la que se pudo resolver la complicación y extraer el cuerpo extraño, sin datos angiográficos de disección, perforación o trombosis, con arteria abierta y razonable flujo distal, persistiendo, eso sí, una estenosis severa del vaso. Se utilizaron los patrones funcionales de Marjory Gordon para llevar a cabo la valoración y resolución de los problemas de salud del paciente aplicando la taxonomía NANDA, NOC, NIC, formulándose, para ello, los diagnósticos de Riesgo de deterioro de la integridad cutánea, Riesgo de disfunción tisular periférica, Riesgo de infección, Riesgo de sangrado y ansiedad. Además, se propusieron unos resultados con sus indicadores evaluables, y unas actividades de enfermería para conseguir solventar dichos problemas. Este caso clínico presenta una complicación poco frecuente, que puede surgir durante un procedimiento de angioplastia, y sirve para resaltar la importancia de una buena planificación de los cuidados.S

Direct toxicity of six antibiotics on soil bacterial communities affected by the addition of bio-adsorbents

Reducing the toxicity caused by antibiotics on bacterial communities in the soil is one of the great challenges of this century. For this, the effectiveness of amending the soil with different bioadsorbents such as crushed mussel shell (CMS), pine bark (PB) and biomass ash (BA), as well as combinations of them (CMS + PB and PB + BA) was studied at different doses (0 g kg−1 to 48 g kg−1). Soil samples were spiked, separately, with increasing doses (0–2000 mg kg−1) of cefuroxime (CMX), amoxicillin (AMX), clarithromycin (CLA), azithromycin (AZI), ciprofloxacin (CIP) and trimethoprim (TMP). Their toxicity on bacterial growth was estimated using the tritium-labeled leucine (3H) incorporation method. Toxicity was observed to behave differently depending on the antibiotic family and bioadsorbent, although in different magnitude and at different doses. The toxicity of β-lactams (AMX and CXM) was reduced by up to 54% when the highest doses of bio-adsorbents were added due to the increase in pH (CMS and BA) and carbon (PB) contribution. Macrolides (CLA and AZI) showed slight toxicity in un-amended soil samples, which increased by up to 65% with the addition of the bio-adsorbents. The toxicity of CIP (a fluoroquinolone) increased with the dose of the bio-adsorbents, reaching up to 20% compared with the control. Finally, the toxicity of TMP (a diaminopyrimidine) slightly increased with the dose of bio-adsorbents. The by-products that increase soil pH are those that showed the highest increases of CLA, AZI, CIP and TMP toxicities. These results could help to prevent/reduce environmental pollution caused by different kinds of antibiotics, selecting the most appropriated bio-adsorbents and doses.Agencia Estatal de Investigación | Ref. RTI2018-099574-B-C21Agencia Estatal de Investigación | Ref. RTI2018-099574-B-C22Ministerio de Economía, Industria y Competitividad | Ref. RYC-2016-20411Xunta de Galicia | Ref. ED481B-2022-081Xunta de Galicia | Ref. ED481A-2021/309Financiado para publicación en acceso aberto: Universidade de Vigo/CISU