Interspecific Germline Transmission of Cultured Primordial Germ Cells

In birds, the primordial germ cell (PGC) lineage separates from the soma within 24 h following fertilization. Here we show that the endogenous population of about 200 PGCs from a single chicken embryo can be expanded one million fold in culture. When cultured PGCs are injected into a xenogeneic embryo at an equivalent stage of development, they colonize the testis. At sexual maturity, these donor PGCs undergo spermatogenesis in the xenogeneic host and become functional sperm. Insemination of semen from the xenogeneic host into females from the donor species produces normal offspring from the donor species. In our model system, the donor species is chicken (Gallus domesticus) and the recipient species is guinea fowl (Numida meleagris), a member of a different avian family, suggesting that the mechanisms controlling proliferation of the germline are highly conserved within birds. From a pragmatic perspective, these data are the basis of a novel strategy to produce endangered species of birds using domesticated hosts that are both tractable and fecund

Historische Grundwissenschaften und die digitale Herausforderung

Unter Federführung von Eva Schlotheuber (Heinrich-Heine-Universität Düsseldorf / VHD-Unterausschuss "Geschichte in der digitalen Welt") und Frank Bösch (Zentrum für die Zeithistorische Forschungen Potsdam / VHD-Unterausschuss "Audiovisuelle Quellen") verabschiedete der VHD ein Grundsatzpapier zum Status der Historischen Grundwissenschaften mit dem Titel "Quellenkritik im digitalen Zeitalter: Die Historischen Grundwissenschaften als zentrale Kompetenz der Geschichtswissenschaft und benachbarter Fächer". Das Grundsatzpapier, in dem auch ein forschungsstrategisches Interesse an den Grundwissenschaften in der digitalen Transformation zum Ausdruck kommt, wurde auf H-Soz-Kult veröffentlicht und mit einem Diskussionsforum begleitet. Dazu wurde aus dem breiten Spektrum der Historischen Kulturwissenschaften eine Reihe in- und ausländischer Kolleginnen und Kollegen zur Kommentierung und Diskussion eingeladen, um die Debatte zu stimulieren

Consensus guidelines for the use and interpretation of angiogenesis assays

The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference

Rahmenbedingen der Marienfrömmigkeit im Späten Mittlelalter

History of Art and Architectur

Magdalena Kremer, Scribe and Painter of the Choir and Chapter Books of the Dominican Convent of St. Johannes-Baptista in Kirchheim unter Teck

History of Art and Architectur

Revelation and Concealment: Apophatic Imagery in the Trinitarian Miniatures of the Rothschild Canticles

Germanic Languages and Literature

The Waddesdon Psalter and the Shop of Jean Pucelle

History of Art and Architectur

Openings

History of Art and Architectur

"To Make Women Weep": Ugly Art as "Feminine" and the Origins of Modern Aesthetics

History of Art and Architectur

Encounter: The Rothschild Canticles

Germanic Languages and Literature