Expression profiling of rainbow trout testis development identifies evolutionary conserved genes involved in spermatogenesis.

Chantier qualité GAInternational audienceBACKGROUND: Spermatogenesis is a late developmental process that involves a coordinated expression program in germ cells and a permanent communication between the testicular somatic cells and the germ-line. Current knowledge regarding molecular factors driving male germ cell proliferation and differentiation in vertebrates is still limited and mainly based on existing data from rodents and human. Fish with a marked reproductive cycle and a germ cell development in synchronous cysts have proven to be choice models to study precise stages of the spermatogenetic development and the germ cell-somatic cell communication network. In this study we used 9K cDNA microarrays to investigate the expression profiles underlying testis maturation during the male reproductive cycle of the trout, Oncorhynchus mykiss. RESULTS: Using total testis samples at various developmental stages and isolated spermatogonia, spermatocytes and spermatids, 3379 differentially expressed trout cDNAs were identified and their gene activation or repression patterns throughout the reproductive cycle were reported. We also performed a tissue-profiling analysis and highlighted many genes for which expression signals were restricted to the testes or gonads from both sexes. The search for orthologous genes in genome-sequenced fish species and the use of their mammalian orthologs allowed us to provide accurate annotations for trout cDNAs. The analysis of the GeneOntology terms therefore validated and broadened our interpretation of expression clusters by highlighting enriched functions that are consistent with known sequential events during male gametogenesis. Furthermore, we compared expression profiles of trout and mouse orthologs and identified a complement of genes for which expression during spermatogenesis was maintained throughout evolution. CONCLUSION: A comprehensive study of gene expression and associated functions during testis maturation and germ cell differentiation in the rainbow trout is presented. The study identifies new pathways involved during spermatogonia self-renewal or rapid proliferation, meiosis and gamete differentiation, in fish and potentially in all vertebrates. It also provides the necessary basis to further investigate the hormonal and molecular networks that trigger puberty and annual testicular recrudescence in seasonally breeding species

Why Bothering to Measure Stellar Rotation with CoRoT?

One important goal of the CoRoT experiment is to obtain information about the internal rotation of stars, in particular the ratio of central to surface rotation rates. This will provide constraints on the modelling of transport mechanisms of angular momentum acting in radiative (rotationally induced turbulent) and convective zones (plumes, extension beyond convectively instable regions). Relations between the surface rotation period and age, magnetic activity, mass loss and other stellar characteristics can also be studied with a statistically significant set of data as will be provided by Corot. We present various theoretical efforts performed over the past years in order to develope the theoretical tools which will enable us to study rotation with Corot

Search for 22^{22}Na in novae supported by a novel method for measuring femtosecond nuclear lifetimes

Classical novae are thermonuclear explosions in stellar binary systems, and important sources of $^{26}$Al and $^{22}$Na. While gamma rays from the decay of the former radioisotope have been observed throughout the Galaxy, $^{22}$Na remains untraceable. The half-life of $^{22}$Na (2.6 yr) would allow the observation of its 1.275 MeV gamma-ray line from a cosmic source. However, the prediction of such an observation requires good knowledge of the nuclear reactions involved in the production and destruction of this nucleus. The $^{22}$Na($p,\gamma$)$^{23}$Mg reaction remains the only source of large uncertainty about the amount of $^{22}$Na ejected. Its rate is dominated by a single resonance on the short-lived state at 7785.0(7) keV in $^{23}$Mg. In the present work, a combined analysis of particle-particle correlations and velocity-difference profiles is proposed to measure femtosecond nuclear lifetimes. The application of this novel method to the study of the $^{23}$Mg states, combining magnetic and highly-segmented tracking gamma-ray spectrometers, places strong limits on the amount of $^{22}$Na produced in novae, explains its non-observation to date in gamma rays (flux < 2.5x$10^{-4}$ ph/(cm$^2$s)), and constrains its detectability with future space-borne observatories.Comment: 18 pages, 3 figures, 1 tabl

Seismic probe of transport processes in red giants

International audienceSeismic data obtained with the space photometric CoRoT and Keplerinstruments have led to a unprecendently precise characterization- interms of masses and ages- of a large sample of post main sequence stars(low mass subgiant and red giants). The high quality of the collectedseismic data and the subsequent theoretical work for interpreting thembrought up a series of issues which revealed that our knowledge of theinternal properties of red giant stars remains quite limited. Two suchimportant issues are discussed here, namely mixing beyond the convectivecore of helium burning red giant stars and evolution of internal angularmomentum for post main sequence stars. This includes how they werediagnosed and what are the resulting improvements in our understandingregarding these issues (or rather how far we are from a properunderstanding and realistic modelling of the structure and evolution ofpost main sequence stars)

Concluding remarks and perspectives on the evolution and seismic tools activity (ESTA) of the CoRoT community

International audienceA first part gives a summary of the main conclusions stemmed from the different comparisons reported in this book. One main conclusion is: with some care in the computations, one is able to obtain frequency differences between different computations which are smaller than required by Corot challenges. This is true for high frequency modes. Small frequency differences are much less easily obtained for low frequencies around the fundamental radial mode frequency. Care here means: having the same physics, same constants of physics, same input stellar parameters, computing models and oscillations with enough self numerical consistent accuracy. The ESTA group has built reference grids of models and associated oscillation frequencies and made them available to the community. In a second part of the present paper, a study case is considered in order to show the need and the use of such reference grids. Finally some perspectives concerning the remaining tasks are suggested

Rotation detection in solar-like stars

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Rotation detection in solar-like stars

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Plato: status and preparation of the mission from the stellar point of view

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Seismology of delta Scuti Stars: Problems and Prospects (invited paper)

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Plato: status and preparation of the mission from the stellar point of view

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