Strategies for Implementing Mindfulness and Mindful Eating into Health and Fitness Professionals’ Practice

Obesity is one of the most pressing public health issues in the United States (U.S.) today as it increases the risk of morbidity and mortality through chronic diseases, including cardiovascular diseases, type 2 diabetes, certain cancers, and mental health conditions (1). Defined by the Centers for Disease Control and Prevention as a body mass index greater than 30 kg·m−2, obesity is the second leading cause of preventable deaths (2). Projected estimates indicate that nearly one in two adults will have obesity and almost one in four adults will have severe obesity by 2030 (3). Further, existing disparities in obesity prevalence by race/ethnicity, socioeconomic position, and geographical region are expected to widen (3,4). Maintaining a healthy weight is determined by the balance of energy intake (food calories from dietary macronutrients) with total energy expenditure (TEE). TEE is composed of the energy cost of one’s basal metabolic rate, the thermic effect of digestion and assimilation of food, and total physical activity. When an individual overeats and/or is sedentary relative to TEE, a positive net energy balance is created, resulting in weight gain. Research indicates that clinical improvements in health outcomes begin to appear with even small amounts of weight loss (5). Programs combining diet and exercise have been shown to result in initial and sustained weight loss (6–8)

Physical Activity Promotion and Support: Meeting Caregivers’ Unique Needs

The benefits of engaging in regular physical activity are extensive and can have a profound positive effect on one’s overall health (1). Numerous recent scientific reviews (2) further underscore the important role physical activity plays in achieving optimal health and preventing noncommunicable disease. For example, physical activity reduces risk of several types of cancer (3), ischemic heart disease, ischemic stroke, heart failure, and premature mortality (4). Among older adults, physical activity can improve cognitive functioning (5), reduce the risk of fall-related injuries, and delay the loss of physical function and mobility (6). According to Healthy People 2030, the nation’s health agenda, one of the goals is to improve the health, fitness, and quality of life for people of all ages through regular physical activity (7). Among the 23 physical activity objectives are aims to increase the proportion of adults who meet current minimum guidelines for aerobic physical activity and muscle-strengthening activity (PA-05) from current estimates of 24.0% to 28.4% and to reduce the proportion of adults who engage in no leisure-time physical activity (PA-01) from 25.4% to 21.2% by the year 2030

Increasing Physical Activity using an Ecological Model

Increasing the proportion of adults in the United States (U.S.) who engage in physical activity is a national priority. Only 29% met the aerobic guideline, 3% met the muscle strengthening guideline only, and 21% met the guidelines for both aerobic and muscle-strengthening activity. Individually oriented physical activity interventions can be intensive and have large effects; however, changes may be temporary unless supportive environments and health-promoting policies are in place. A variety of strategies are required to get and keep people physically active

Safety concerns in football: An analysis on the protocol of return to play after concussion

The purpose of this article was two-fold: (1) to address and highlight the standards of concussion management protocol provided by the NATA, CDC and major sports governing bodies, and (2) to examine how concussion protocols of different levels of athletic programs and organizations adhere to the proposed guidelines and standards. According to the literature, an ideal concussion management plan must cover elements such as: concussion education, responsibility and information about student athletes’ self-reporting signs and symptoms, policy and rules on restricting activities of athletes who are diagnosed with a concussion, requirements and procedures for diagnosis of a concussion and clearance before returning to play (RTP), baseline testing for athletes, return to learn guidelines, and a stepwise RTP progression. The results of our analysis revealed that most of the programs had complied with the NATA concussion treatment protocol and standards, particularly on the element of the stepwise progression RTP protocol. Implications and practical suggestions are discussed to help improve existing protocols to better serve injured athletes and protect all athletes from further exposure to sport related concussions (SRC)

Comparative grading scales, statistical analyses, climber descriptors and ability grouping: International Rock Climbing Research Association position statement

The research base for rock climbing has expanded substantially in the past 3 decades as worldwide interest in the sport has grown. An important trigger for the increasing research attention has been the transition of the sport to a competitive as well as recreational activity and the potential inclusion of sport climbing in the Olympic schedule. The International Rock Climbing Research Association (IRCRA) was formed in 2011 to bring together climbers, coaches and researchers to share knowledge and promote collaboration. This position statement was developed during and after the 2nd IRCRA Congress which was held in Pontresina, in September 2014. The aim of the position statement is to bring greater uniformity to the descriptive and statistical methods used in reporting rock climbing research findings. To date there is a wide variation in the information provided by researchers regarding the climbers’ characteristics and also in the approaches employed to convert from climbing grading scales to a numeric scale suitable for statistical analysis. Our paper presents details of recommended standards of reporting that should be used for reporting climber characteristics and provides a universal scale for the conversion of climbing grades to a number system for statistical analysis

Highly Parallel Genome-Wide Expression Analysis of Single Mammalian Cells

We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs.The method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming sites at both ends of the synthesized cDNA for global PCR amplification. Coupled with a whole-genome gene expression microarray platform, we routinely obtain expression correlation values of R(2)~0.76-0.80 between individual cells and R(2)~0.69 between 50 pg total RNA replicates. Expression profiles generated from single cells or 50 pg total RNA correlate well with that generated with higher input (1 ng total RNA) (R(2)~0.80). Also, the assay is sufficiently sensitive to detect, in a single cell, approximately 63% of the number of genes detected with 1 ng input, with approximately 97% of the genes detected in the single-cell input also detected in the higher input.In summary, our method facilitates whole-genome gene expression profiling in contexts where starting material is extremely limiting, particularly in areas such as the study of progenitor cells in early development and tumor stem cell biology

Imaging biomarker roadmap for cancer studies.

Imaging biomarkers (IBs) are integral to the routine management of patients with cancer. IBs used daily in oncology include clinical TNM stage, objective response and left ventricular ejection fraction. Other CT, MRI, PET and ultrasonography biomarkers are used extensively in cancer research and drug development. New IBs need to be established either as useful tools for testing research hypotheses in clinical trials and research studies, or as clinical decision-making tools for use in healthcare, by crossing 'translational gaps' through validation and qualification. Important differences exist between IBs and biospecimen-derived biomarkers and, therefore, the development of IBs requires a tailored 'roadmap'. Recognizing this need, Cancer Research UK (CRUK) and the European Organisation for Research and Treatment of Cancer (EORTC) assembled experts to review, debate and summarize the challenges of IB validation and qualification. This consensus group has produced 14 key recommendations for accelerating the clinical translation of IBs, which highlight the role of parallel (rather than sequential) tracks of technical (assay) validation, biological/clinical validation and assessment of cost-effectiveness; the need for IB standardization and accreditation systems; the need to continually revisit IB precision; an alternative framework for biological/clinical validation of IBs; and the essential requirements for multicentre studies to qualify IBs for clinical use.Development of this roadmap received support from Cancer Research UK and the Engineering and Physical Sciences Research Council (grant references A/15267, A/16463, A/16464, A/16465, A/16466 and A/18097), the EORTC Cancer Research Fund, and the Innovative Medicines Initiative Joint Undertaking (grant agreement number 115151), resources of which are composed of financial contribution from the European Union's Seventh Framework Programme (FP7/2007-2013) and European Federation of Pharmaceutical Industries and Associations (EFPIA) companies' in kind contribution

A framework for human microbiome research

A variety of microbial communities and their genes (the microbiome) exist throughout the human body, with fundamental roles in human health and disease. The National Institutes of Health (NIH)-funded Human Microbiome Project Consortium has established a population-scale framework to develop metagenomic protocols, resulting in a broad range of quality-controlled resources and data including standardized methods for creating, processing and interpreting distinct types of high-throughput metagenomic data available to the scientific community. Here we present resources from a population of 242 healthy adults sampled at 15 or 18 body sites up to three times, which have generated 5,177 microbial taxonomic profiles from 16S ribosomal RNA genes and over 3.5 terabases of metagenomic sequence so far. In parallel, approximately 800 reference strains isolated from the human body have been sequenced. Collectively, these data represent the largest resource describing the abundance and variety of the human microbiome, while providing a framework for current and future studies

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis

Publisher Copyright: © 2022, The Author(s).Background: Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery. Results: To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N = 1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3–5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism. Conclusions: Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.Peer reviewe