Beetles belonging to the family bruchidae deposited in the entomological collection of the institute of ecology and systematics, La Habana, Cuba

  En el presente trabajo se aporta información sobre el volumen y la composición taxonómica de Bruchidae (Coleoptera) depositados en la colección entomológica del Instituto de Ecología y Sistemática, La Habana, Cuba. Se ofrece su inventario, aportando el número de especies, géneros, ejemplares y datos contenidos en las etiquetas adjuntas al ejemplar. La colección contiene 318 ejemplares, 13 géneros y 31 especies distribuidas en tres subfamilias. De las 13 morfoespecies presentes, tres están identificadas hasta género y 10 hasta familia. Se incluye una lista de especies con datos sobre las localidades, fecha de recolecta, recolectores y en algunos casos la planta hospedera. La colección Básica contiene un mayor número de especies y ejemplares, mientras que la de Gundlach la supera en endémicos y tipos depositados. Los ejemplares fueron recolectados en 13 de las 15 provincias y el municipio especial Isla de la Juventud; las provincias que tienen mayor número de especies son La Habana y Artemisa. La etapa con mayor número de capturas es la de 1916 a 1939 (17 especies y 96 ejemplares).  The present paper gives information on volume and taxonomic composition of Bruchidae (Coleoptera) deposited in the entomological collection of the Ecology and Systematics Institute, La Habana, Cuba. The inventory includes number of species, genera and specimens, as well as data of labels attached to specimen. The collection contains 318 specimens, 13 genera and 31 species included in three subfamilies. Of the 13 morphospecies, three are identified until genera and 10 to family. A list of species with data about localities, date of collecting and in some cases the host plant are included. The Basic collection contains more number of species and specimens than Gundlach collection which surpasses in endemic and types deposited. The specimens were captured in 13 of the 15 provinces and the special municipality Isle of Young; Provinces with more specimens are La Habana and Artemisa. The period of more specimens collected was from 1916 to 1939 (17 species and 96 specimens

Collection of herpetology of the institute of ecology and systematics, la Habana, Cuba. Family dipsadidae. Genera arrhyton y cubophis; family boidae, genus chilabothrus

Snakes of the genera Arrhyton, Cubophis, and Chilabothrus were examined at the herpetological collection of the Ecology and Systematics Institute, La Habana, Cuba. Several mistakes in the numbers of labels and cards were found that were repaired. Starting from the reparation we prepared 123 new cards and 61 new labels; 65 specimens were catalogued and 110 were included in an Excel sheet for the first time. Digital text catalogue were prepared. Six of the eight species of the genus Arrhyton were detected, with 74 specimens obtained in 12 of the 15 provinces and the Isla de la Juventud. Provinces with more specimens are La Habana and Artemisa. The four Cuban subspecies of the genus Cubophis are in the collection, with 227 specimens captured in the 15 provinces and the Isla de la Juventud. La Habana province has more specimens and Las Tunas and Granma have only one specimen each other. There are 38 specimens of Chilabothrus angulifer from nine of the 15 provinces and the Isla de La Juventud. Pinar del Río, Santiago de Cuba and Ciego de Ávila are the provinces with more specimens whereas Artemisa, Cienfuegos and Granma have one specimen in each of them. Collectors who have contributed to capture more specimens are Luis V. Moreno (88), Orlando H. Garrido (39), Julio Novo (35) and Miguel L. Jaume (18). The period of more specimens collected was from 1960 to 1989 (163) and the lesser captures were from 2000 to present (19)

Colección herpetológica del instituto de ecología y sistemática, la Habana, Cuba. Familia teiidae, género ameiva

Lizards of the genus Ameiva were examined at the herpetological collection of the Ecology and Systematics Institute, La Habana, Cuba. Several mistakes in the numbers of labels and cards were found that were repaired. Starting from the reparation we prepared 93 new cards and 294 new labels; 51 specimens were catalogued and were included in an Excel sheet for the first time. Digital text catalogue were prepared. All the 28 subspecies of the genus described for Cuba were detected, with 553 specimens obtained in the 15 provinces and the Isla de la Juventud. Provinces with more number of specimens are Pinar del Río and Matanzas, with lesser numbers in La Habana and Artemisa. Collectors who have contributed with more collections are Orlando H. Garrido (183), Miguel L. Jaume (141) and Luis V. Moreno (115). The period of more captures was from 1960 to 1979 (475) and the lesser captures were from 2000 to present (15).   Se realizó el inventario físico de los ejemplares del género Ameiva presentes en la colección herpetológica del Instituto de Ecología y Sistemática, La Habana, Cuba. Se detectaron errores en números de etiquetas y tarjetas repetidas o faltantes. A partir de arreglar los errores se confeccionaron 93 tarjetas y 224 etiquetas nuevas; se catalogaron 51 ejemplares que se introdujeron en hojas de Excel; se preparó el catálogo en procesador de texto y soporte digital. Se detectaron las 28 subespecies del género descritas para Cuba, con 553 especímenes recolectados en las 15 provincias y el municipio especial Isla de la Juventud; las provincias que tienen mayor número son Pinar del Río y Matanzas y menor número La Habana y Artemisa. Los recolectores que han aportado más ejemplares son Orlando H. Garrido (183), Miguel L. Jaume (141) y Luis V. Moreno (115). La etapa en que ha habido mayor número de capturas es la de 1960 a 1979 (475) y la de menor, entre el año 2000 y el presente (15)

Colección herpetológica del instituto de ecología y sistemática, la Habana, Cuba. Familia dipsadidae, géneros caraiba y tretanorhinus; familia natricidae, género nerodia

Se realizó el inventario físico de las serpientes de los géneros Caraiba, Tretanorhinus y Nerodia presentes en la colección herpetológica del Instituto de Ecología y Sistemática, La Habana, Cuba. Se detectaron errores en números de etiquetas y tarjetas repetidas o faltantes. A partir de arreglar los errores se confeccionaron 61 tarjetas y 88 etiquetas nuevas; se catalogaron 61 ejemplares; se introdujeron 74 registros nuevos en hojas de Excel; se preparó el catálogo en procesador de texto y soporte digital. Se detectaron las seis subespecies del género Caraiba, con 220 ejemplares recolectados en las 15 provincias y el municipio especial Isla de la Juventud; las provincias que tienen mayor número son La Habana y Pinar del Río. Del género Tretanorhinus se encontraron tres de las cuatro subespecies cubanas con 62 ejemplares capturados en 7 de las 15 provincias y en el municipio especial Isla de la Juventud; las provincias de mayor número de ejemplares son Artemisa y Matanzas y las de menor número, Villa Clara y Ciego de Ávila. Nerodia clarkii está presente con 20 especímenes de 6 de las 15 provincias. Sancti Spíritus es la provincia de mayor número de capturas. Los recolectores que han aportado más ejemplares de los tres géneros son Luis V. Moreno (66), Julio Novo (42), Orlando H. Garrido (31) y Miguel L. Jaume (24). La etapa en que ha habido mayor número de recolectas es la de 1960 a 1969 (122), seguida de la de 1970 a 1989 (98) y la de menor, entre el año 2000 y el presente (1

Ensayo cuantitativo para la determinación de la actividad hidrolítica de la enzima biotinidasa

A quantitative assay was standardized to determine the hydrolytic activity of biotinidase in human serum samples. The current Wolf et al. assay uses N-biotinyl-p-aminobenzoic acid as substrate. The assay's precision was improved by the addition of detergents to the sodium nitrite solution. This eliminated the formation of nitrogen bubbles during the diazotation reaction. Freezing and thawing of the serum samples did not affect the hydrolytic activity Farmaco's interference test showed positive results with sulfametoxasol/trimetoprima and procainel benzylpenicilline. The hydrolytic activity of biotinidase averaged 7.04 + 2.2 nmol/min/ml in a group of 205 healthy children. No statistically significant differences in enzymatic activity was observed between variables of sex, races and age group. The standardize assay can determine the hydrolytic activity of biotinidase human serum samples and can confirm the positive cases detected by neonatal screening programs for biotinidase deficiency.El presente trabajo describe la estandarización de un ensayo diseñado para cuantificar la actividad hidrolítica de la enzima biotinidasa en muestras de suero humano, el cual se basa en el método de Wolf y colaboradores, que emplea el N-biotinil-p-aminobenzoico (BPABA) como sustrato. Con la adición de detergentes a la solución de nitrito de sodio, se eliminan las burbujas de nitrógeno formadas durante la reacción de diazotación y se mejora la precisión del ensayo. Se observó que la congelación-descongelación de las muestras de suero no afecta la actividad hidrolitica de la enzima biotinidasa. La evaluación de la interferencia de fármacos en el ensayo mostró que con el sulfametoxasol/trimetoprim y la procainal benzilpenicilina hay desarrollo de color en ausencia del sustrato BPABA. Los valores promedio de actividad hidrolitica de la biotinidasa, obtenidos en un grupo de 205 niños sanos, fue de 7,04 + 2,2 nmol/min/ml. No se encontraron diferencias estadísticamente significativas al evaluar la actividad de la enzima por sexo, raza y grupos de edad. Este ensayo se puede emplear en la determinación de la actividad hidrolítica de la enzima biotinidasa en muestras de suero humano y, específicamente, en la confirmación de los casos detectados por los programas de tamizaje neonatal para la deficiencia de biotinidasa

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Correction to: Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

The original version of this article unfortunately contained a mistake

Colección herpetológica del instituto de ecología y sistemática, La Habana, Cuba. familia tropidophiidae, género tropidophis

Snakes of the genus Tropidophis were examined at the herpetological collection of the Ecology and Systematics Institute, La Habana, Cuba. Several mistakes in the numbers of labels and cards were found that were repaired. Starting from the reparation were prepared 73 new cards and 125 new labels; 90 specimens were catalogued and 118 were included in the Excel sheet for the first time. Digital text catalogue were prepared. Of the 16 described species from Cuba, 14 were detected, with 322 specimens captured in the 15 provinces and the Isla de La Juventud. Provinces with greatest numbers of specimens are La Habana, Pinar del Río and Artemisa, whereas Cienfuegos and Granma are the ones with lesser numbers (2). Collectors that have contributed to capture more specimens are Luis V. Moreno (66), Julio Novo (46), Miguel L. Jaume (32) y Orlando H. Garrido (21). The period of more specimens collected was from 1970 to 1989 (119) and the lesser captures were from 2000 and the present (21).Se realizó el inventario físico de las serpientes del género Tropidophis presentes en la colección herpetológica del Instituto de Ecología y Sistemática, La Habana, Cuba. Se detectaron errores en números de etiquetas y tarjetas repetidas o faltantes. A partir de arreglar los errores se confeccionaron 73 tarjetas y 125 etiquetas nuevas; se catalogaron 90 ejemplares; se introdujeron 118 registros nuevos en hojas de Excel; se preparó el catálogo en procesador de texto en soporte digital. En la colección están 14 de las 16 especies del género descritas para Cuba. Se hallaron 322 ejemplares, recolectados en las 15 provincias y el municipio especial Isla de la Juventud. Las provincias que tienen mayor número son La Habana, Pinar del Río y Artemisa y las de menores números son Cienfuegos y Granma (2). Los recolectores que han aportado más ejemplares son Luis V. Moreno (66), Julio Novo (46), Miguel L. Jaume (32) y Orlando H. Garrido (21). La etapa en que ha habido mayor número de capturas es la de 1970 a 1989 (119) y la de menor, entre el año 2000 y el presente (21)