Morphogenesis and Defoliation Frequency of Italian Ryegrass (\u3ci\u3eLolium multiflorum\u3c/i\u3e) and \u3ci\u3ePaspalum urvillei\u3c/i\u3e in Lowland under Three Levels of P and K

This trial was conducted to evaluate growth dynamics and defoliation frequency of Italian ryegrass (Lolium multiflorum) and Paspalum urvillei in a lowland area under three levels of P and K. Using randomization tests we observed that, in a general way, fertilizer treatments were not the determinant factors of leaf appearance and elongation rates for both species. Only the leaf appearance rate of Paspalum urvillei at 100 % of recommendation level and the elongation rate for Italian ryegrass, in the same treatment, was different. Probably other factors, like drainage, are contributing to these results. I. ryegrass phyllochron ranged from 57.3 to 72.6 degree-days. Paspalum urvillei presented higher grazing frequency in almost all evaluated periods, revealing a high preference by the grazing animals

Comunicação científica e o protocolo OAI: uma proposta na área das Ciências da Comunicação

A comunicação científica se mostra imprescindível para o desenvolvimento da ciência e das atividades dos cientistas. Neste contexto, este trabalho busca analisar um novo formato de publicação eletrônica (mais especificamente o repositório digital ‘open archives’), partindo de uma reflexão sobre o sistema tradicional de comunicação científica, de modo a definir um projeto de planejamento e implementação de um repositório temático na área de Ciências da Comunicação. Em síntese, descreve-se o caminho percorrido pela equipe da PORTCOM (Rede de Informação em Ciências da Comunicação dos Países de Língua Portuguesa) durante a fase inicial de concepção do projeto Arena Científica junto aos pesquisadores dos núcleos de pesquisa da INTERCOM (Sociedade Brasileira de Estudos Interdisciplinares da Comunicação). Neste texto, apresenta-se resumidamente conceitos e definições que norteiam a estrutura dos arquivos abertos

Chromomycin A2 Induces Autophagy in Melanoma Cells

The present study highlights the biological effects of chromomycin A2 toward metastatic melanoma cells in culture. Besides chromomycin A2, chromomycin A3 and demethylchromomycin A2 were also identified from the extract derived from Streptomyces sp., recovered from Paracuru Beach, located in the northeast region of Brazil. the cytotoxic activity of chromomycin A2 was evaluated across a panel of human tumor cell lines, which found IC50 values in the nM-range for exposures of 48 and 72 h. MALME-3M, a metastatic melanoma cell line, showed the highest sensitivity to chromomycin A2 after 48h incubation, and was chosen as a model to investigate this potent cytotoxic effect. Treatment with chromomycin A2 at 30 nM reduced cell proliferation, but had no significant effect upon cell viability. Additionally, chromomycin A2 induced accumulation of cells in G(0)/G(1) phase of the cell cycle, with consequent reduction of S and G(2)/M and unbalanced expression of cyclins. Chromomycin A2 treated cells depicted several cellular fragments resembling autophagosomes and increased expression of proteins LC3-A and LC3-B. Moreover, exposure to chromomycin A2 also induced the appearance of acidic vacuolar organelles in treated cells. These features combined are suggestive of the induction of autophagy promoted by chromomycin A2, a feature not previously described for chromomycins.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico (FUNCAP-Programa Areas Estrategicas)Univ Fed Ceara, Inst Marine Sci, BR-60165081 Fortaleza, Ceara, BrazilUniv Fed Ceara, Dept Physiol & Pharmacol, BR-60430270 Fortaleza, Ceara, BrazilUniversidade Federal de São Paulo, Dept Marine Sci, BR-11030400 São Paulo, BrazilUniv Fed Ceara, Dept Organ & Inorgan Chem, BR-60021970 Fortaleza, Ceara, BrazilFdn MEDINA, Ctr Excelencia Invest Medicamentos Innovadores An, Granada 18016, SpainUniversidade Federal de São Paulo, Dept Marine Sci, BR-11030400 São Paulo, BrazilWeb of Scienc

Caracterização da cama utilizada em sistemas Compost Barn : Characterization of the beds used in compost Barn Systems

O sistema de criação compost barn apresenta crescente implantação em nosso país, este sistema consiste em alojar vacas leiterias em galpões, onde o piso é revestido por cama, material de origem orgânica e higroscópico, sobre esta cama os animais permanecem soltos. Na cama é onde ocorre o processo de compostagem aeróbia, o qual é induzido pela periódica homogeneização dos dejetos dos animais, ali depositados, associados à aeração rotineira que o sistema exige. Sabendo que as condições química e física da cama influencia o processo de compostagem destas camas, o objetivo do presente estudo consistiu na caracterização química e física da cama em uso em um galpão compost barn. O estudo foi realizado em uma unidade produtora de leite localizada na região da Zona da Mata Mineira, as amostras foram coletadas e submetidas às análises laboratoriais de: pH, teor de água e de matéria orgânica e relação carbono:nitrogênio (C:N). Os dados foram analisados na forma descritiva. E então, nas condições ambientais e temporais estudadas, a cama do galpão compost barn apresentou valores de pH que favorecem a atividade microbiana. O teor de água apresentou-se abaixo da faixa ideal para a compostagem, porém, mantém a salubridade do ambiente e dos animais. O teor de matéria orgânica e a relação apresentaram valores dentro das faixas preconizadas pela literatura

Hyperbaric oxygen therapy ameliorates TNBS-induced acute distal colitis in rats

Abstract\ud \ud Background\ud This study investigated the therapeutic effects of hyperbaric oxygen in experimental acute distal colitis focusing on its effect on the production of pro-inflammatory cytokines, nitric oxide and hypoxia-inducible factor 1alpha.\ud \ud \ud Methods\ud Colitis was induced with a rectal infusion of 150 mg/kg of TNBS under anesthesia with Ketamine (50 mg/kg) and Xylazine (10 mg/kg). Control animals received only rectal saline. After colitis induction, animals were subjected to two sessions of hyperbaric oxygen and were then euthanized. The distal intestine was resected for macroscopic analysis, determination of myeloperoxidase activity, western-blotting analyses of inducible nitric oxide synthase and cyclooxygenase-2 expression and immunohistochemical analysis of hypoxia-inducible factor 1alpha and cyclooxygenase-2. Cytokines levels in the distal intestine were measured using an enzyme-linked immunosorbent assay.\ud \ud \ud Results\ud Hyperbaric oxygen therapy attenuated the severity of acute distal colitis, with reduced macroscopic damage score. This effect was associated with prevention in the increase of pro-inflammatory cytokine production; myeloperoxidase activity, in the expression of inducible nitric oxide synthase and cyclooxygenase-2. Finally, hyperbaric oxygen inhibited the acute distal colitis-induced up-regulation of hypoxia-inducible factor 1alpha.\ud \ud \ud Conclusions\ud The results indicate that hyperbaric oxygen attenuates the severity of acute distal colitis through the down-regulation of pro-inflammatory events.This work was supported by FAEPA\ud (Fundação de Apoio ao Ensino, Pesquisa e Assistência), Ribeirão Preto\ud Medical School University of Sao Paulo, Brazil and Grant 2011/19670-0 from\ud São Paulo Research Foundation (FAPESP)

SEROLOGICAL DETECTION OF HEPATITIS A VIRUS IN FREE-RANGING NEOTROPICAL PRIMATES (Sapajus spp., Alouatta caraya) FROM THE PARANÁ RIVER BASIN, BRAZIL

Nonhuman primates are considered as the natural hosts of Hepatitis A virus (HAV), as well as other pathogens, and can serve as natural sentinels to investigate epizootics and endemic diseases that are of public health importance. During this study, blood samples were collected from 112 Neotropical primates (NTPs) (Sapajus nigritus and S. cay, n = 75; Alouatta caraya, n = 37) trap-captured at the Paraná River basin, Brazil, located between the States of Paraná and Mato Grosso do Sul. Anti-HAV IgG antibodies were detected in 4.5% (5/112) of NTPs, specifically in 6.7% (5/75) of Sapajus spp. and 0% (0/37) of A. caraya. In addition, all samples were negative for the presence of IgM anti-HAV antibodies. These results suggest that free-ranging NTPs were exposed to HAV within the geographical regions evaluated

Sentido social del hábitat. IV Congreso sobre Arquitectura y Cooperación al Desarrollo: libro de resúmenes

Resúmenes del IV Congreso sobre Arquitectura y Cooperación al Desarrollo, celebrado los días 29 y 30 de septiembre de 2016 en A Coruña