Variations of the abductor pollicis longus tendon: an anatomic study

This study was performed to investigate the variations, dimensions and attachment of the abductor pollicis longus (APL) tendon. Fifty upper limbs of adult cadavers were dissected in the Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. Variations of APL tendon and its way of insertion were observed;its length, width and thickness were also measured and statistically analysed. Nosingle-tendon-APL was found in all the examined upper limbs. The APL exhibited 2–6 tendons in all the cases. Tendons of APL were classified into medial and lateral. The lateral was considered as the main APL tendon, which was inserted into thebase of the first metacarpal bone. The medial tendons were regarded as accessory slips and were inserted most commonly into the base of the first metacarpalbone; 80% on its anterolateral surface vs. 20% on the lateral one. The rest of themedial slips extended to trapezium, abductor pollicis brevis, thenar fascia, capsuleof first carpometacarpal joint and opponens pollicis, with a frequency of 80%,60%, 40%, 30% and 20%, respectively. Transverse tendinous fibres were noted connecting the various slips of the APL tendons. No significant difference was recorded between the length of the main APL tendon and its accessory tendons, however, a highly significant difference was found between their thicknesses aswell as their widths. Thus it is verified that the lateral tendon of APL is the main,whereas the medial tendons are accessory. The observations regarding dimensions and variations of the APL and their accessory tendons may prove useful tosurgeons as a source of grafting material

Anatomical variations of the hand extensors

This study was performed to investigate the anatomy and variations of the humanextensor tendons of the fingers and their intertendinous connections. Ninetyfive upper limbs of adult cadavers were dissected. The variations in the extensortendons of the fingers, both proximal and distal to the extensor retinaculum, andtheir mode of insertion were observed. Also, the intertendinous connections wereexplored and the obtained data were analysed. The extensor pollicis longus andbrevis tendons were found to be single, doubled or, rarely, absent. Their insertioncould be traced to either the proximal phalanx, or through the extensor expansionto both phalanges, or rarely to the distal phalanx of thumb. The extensor indicishad a single tendon in all specimens. In the majority of specimens, extensor digitorumhad no independent slip to the little finger; it gave off a single tendonto the index, double tendons to the middle finger and triple tendons to the ringfinger. Extensor digiti minimi muscle often had double or triple tendons distal tothe extensor retinaculum. Three types of juncturae tendinum (JT) were identifiedbetween the tendons of extensor digitorum in the 2nd, 3rd and 4th intermetacarpalspaces (IMS) of hands. Types 1 and 2 JT were seen in the three IMS. Type 3 JTwas the most frequently identified of all juncturae and was always absent in the2nd IMS. The percentages of the present data were compared with other researchers’data

Crocin mitigates carbon tetrachloride-induced liver toxicity in rats

AbstractObjectivesCarbon tetrachloride (CCl4) is one of the most dangerous hepatotoxic environmental pollutants thus this study aimed at investigating the potential preventive effect and mechanism of crocin against CCl4-induced hepatotoxicity.MethodsForty Male rats were allocated for two weeks treatment with; corn oil, CCl4 in corn oil, crocin (100mg/kg), or crocin plus CCl4. At time of euthanasia liver was removed, weighted and processed for histopathological evaluation and estimation of liver contents of active caspase3, lipid peroxidation (MDA) and reduced glutathione (GSH). We also evaluated antioxidant enzymes activities [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)], phase I metabolizing enzyme [cytochrome P450 sub family 2E1 (CYP2E1)] an Phase II metabolizing enzyme, [glutathione-S-transferase (GST)] in liver tissue. Blood samples were used for evaluation of liver function tests and inflammatory cytokines [interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α)].ResultsCCl4 induced significant (p < 0.001), increase in: relative liver weight to body weight, liver MDA content, liver active caspase-3 and plasma levels of IL-6 and TNF-α. In addition, CCl4 disturbed liver histology, liver metabolizing enzymes (CYP2E1 and GST), and liver function tests (aspartate aminotransferase, alanine aminotransferase, total bilirubin and alkaline phosphatase). CCl4 induced significant decrease in activities of SOD, CAT, GSH-Px and GSH content. Administration of crocin with CCl4 mitigated all CCl4-disturbed parameters and preserved liver histology close to normal.ConclusionCrocin ameliorated CCl4-induced liver injury via inhibition of inflammatory cytokines, caspase3 and oxidative stress along with modulation of liver metabolizing enzymes favoring elimination of CCl4 toxic metabolite

Physostigmine: A Plant Alkaloid Isolated from Physostigma venenosum: A Review on Pharmacokinetics, Pharmacological and Toxicological Activities

Medicinal plants have been documented as an important source for discovering new pharmaceutical molecules that have been used to treat serious diseases. Strikingly, previous reports stated that natural products and their derived compounds exhibit lesser side effects and improved efficacy than other synthetic counterparts. Physostigmine, a parasympathomimetic plant alkaloid isolated from the West African perennial shrub Physostigma venenosum, it shows a narrow therapeutic index and a short life span, despite its ability to penetrate the blood-brain barrier (BBB). It is a widely known reversible butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE) inhibitor and has been documented to treat various ailments such as Alzheimer’s disease. Pharmacologically, physostigmine was first reported as an antidote for atropine scopolamine and belladonna alkaloids toxicity. Recently, it has been documented as a therapy for treating several ailments including glaucoma, myasthenia gravis and the intoxication caused by tricyclic antidepressant overdoses, anti-histamines, antipsychotics, and benzodiazepines. Physostigmine has been reported to be absorbed from the gastrointestinal tract and showed short half-life, as, after the oral administration of 2 mg of physostigmine salicylate, the peak plasma concentration reached to 30 minutes. This review examines the biological activities, pharmacokinetics, and toxicity of physostigmine extracted from P. venenosum. Keywords: Physostigma venenosum, Physostigmine, pharmacological activities, acetylcholinesterase and butyrylcholinesterase inhibitor

Effect of Tea Polyphenol on Oxidative Injury in S180 Cells Induced Hepatocarcinoma Mice

The purpose of this study was to evaluate the antioxidant nature of tea polyphenol on S180 cells induced liver cancer in mice. In the present study, hepatocellular carcinoma was induced by tumor transplantation of liver in situ. The antitumor activity of tea polyphenol has been determined in vivo in hepatocellular carcinoma mice after treatment of drug (50, 100, 150 mg/kg body weight) by gavage for 20 days. Results showed that a significant increase in serum aspartate transaminase (AST), alkaline phosphatase (ALP), alanine aminotransfere (ALT), malondialdehyde (MDA) level, decrease in serum white blood cells (WBC), serum total protein (TP), albumin (ALB), A/G, tumor necrosis factor-α (TNF-α) and interferon-gamma (IFN-γ), liver reduced glutathione (GSH) levels were observed. In addition, the levels of enzymic and non-enzymic antioxidants were decreased when subjected to S180 cells induction. These altered enzyme levels were ameliorated significantly by administration of tea polyphenol at the concentration of 50, 100, 150 mg/kg body weight in drug-treated animals. These results indicate that the protective effect of tea polyphenol was associated with inhibition of MDA induced by S180 cells and to maintain the antioxidant enzyme levels

Dietary antioxidant supplementation promotes growth in senegalese sole postlarvae

Somatic growth is a balance between protein synthesis and degradation, and it is largely influenced by nutritional clues. Antioxidants levels play a key role in protein turnover by reducing the oxidative damage in the skeletal muscle, and hence promoting growth performance in the long-term. In the present study, Senegalese sole postlarvae (45 days after hatching, DAH) were fed with three experimental diets, a control (CTRL) and two supplemented with natural antioxidants: curcumin (CC) and grape seed (GS). Trial spanned for 25 days and growth performance, muscle cellularity and the expression of muscle growth related genes were assessed at the end of the experiment (70 DAH). The diets CC and GS significantly improved growth performance of fish compared to the CTRL diet. This enhanced growth was associated with larger muscle cross sectional area, with fish fed CC being significantly different from those fed the CTRL. Sole fed the CC diet had the highest number of muscle fibers, indicating that this diet promoted muscle hyperplastic growth. Although the mean fiber diameter did not differ significantly amongst treatments, the proportion of large-sized fibers (>25 μm) was also higher in fish fed the CC diet suggesting increased hypertrophic growth. Such differences in the phenotype were associated with a significant up-regulation of the myogenic differentiation 2 (myod2) and the myomaker (mymk) transcripts involved in myocyte differentiation and fusion, respectively, during larval development. The inclusion of grape seed extract (GS diet) resulted in a significant increase in the expression of myostatin1. These results demonstrate that both diets (CC and GS) can positively modulate muscle development and promote growth in sole postlarvae. This effect is more prominent in CC fed fish, where increased hyperplastic and hypertrophic growth of the muscle was associated with an upregulation of myod2 and mymk genes.FCT: IF/00482/2014/CP1217/CT0005; UIDB/04326/2020; VALORMAR (ref. 024517) through Compete 2020, Lisboa 2020, CRESC Algarve 2020, Portugal 2020 and the European Union’s ERDF, and IFAPA project RTA2017-00054-C03-01 funded from MCIU/AEI/FEDER, UE.info:eu-repo/semantics/publishedVersio

Immunological and parasitological parameters after treatment with dexamethasone in murine Schistosoma mansoni

This work aimed to evaluate the effect of diphenyl dimethyl bicarboxylate (DDB) and dexamethasone alone and in combination with praziquantel on various parasitological, immunological and pathological parameters reflecting disease severity and morbidity in murine schistosomiasis. DDB and dexamethasone had no effect on worm burden but altered tissue egg distribution. This indicates that, under the schedule used, neither drug interfered with the development of adult worms or oviposition, but both can modulate liver pathology. Dexamethasone resulted in a greater reduction in granuloma size than did DDB. Dexamethasone-treated mice also showed lower levels of serum gamma interferon (IFN-&#947;), interleukin-12 (IL-12) and IL-4, together with higher IL-10 levels, than infected untreated control animals. These data suggest that dexamethasone is a convenient and promising coadjuvant agent that results in decreased morbidity in murine schistosomiasis