Varietal Preferences of Erythroneura Leafhoppers (Homoptera: Cicadellidae) Feeding on Grapes in New York

Species composition of Erythroneura leafhoppers infesting the 3 major classes of grapes grown in New York was investigated. Eastern grape leafhopper, E. comes (Say), comprised 74-100% of populations collected on the native American (Vitis labrusca Bailey) cultivars ‘Concord', ‘Niagara', ‘Catawba' and ‘Delaware'. On interspecific hybrid (Vitis sp.) and Vitis vinifera L. cultivars, E. comes was largely absent, and 97-100% of leafhoppers collected were 2 cryptic species, E. bistrata McAtee and E. vitifex Fitch. On the native American variety ‘Elvira', a V. labrusca X V riparia Michaux hybrid, field populations were 24% E. comes and 74% the E. bistrata/vitifex complex. E. vitis (Harris), E. tricinta Fitch, and E. vulnerata Fitch were also present in commercial grapes, but never exceeded 20% of the populations sampled. Populations on wild V. riparia adjacent to vineyards were comprised of 24% E. comes, 47% E. histrata/vitifex, 19% E. vitis, and 10% E. tricinta. Dissection revealed that proportions of E. bistrata and E. vitifex in field collections, varied from 97% E. bistrata to 61% E. vitifex. Oviposition of E. comes and E. bistrata on V. vinifera, interspecific hybrid, and native American cultivars was compared in greenhouse choice tests and field no-choice tests. In choice tests, E. comes laid more eggs on Concord and Elvira than on the interspecific hybrid ‘Seyval blanc', or the V. vinifera cultivar ‘White Riesling'. E. bistrata did not oviposit on Concord when paired with either Elvira, Seyval blanc or White Riesling. When caged to grape leaves in no-choice tests, E. comes laid the most eggs on native American cultivars and the fewest on V. vinifera and interspecific hybrids; E. bistrata laid the most eggs on hybrid and V. vinifera cultivars, and very few eggs on three native American cultivars. These results show that E. bistrata and E. vitifex are the principal pest species on V. vinifera and many interspecific hybrid cultivars in New York, and that E. comes is the principal leafhopper pest on native American V. labrusca cultivar

Influence of Temperature-Driven Phenology and Photoperiodic Induction of Reproductive Diapause on Population Dynamics of Erythroneura comes (Homoptera: Cicadellidae)

The influence of degree-day accumulations and photoperiodic induction of diapause on the phenology of Erythroneura Comes (Say) was investigated. In growth chamber experiments, nymphs reared at photoperiods of 710 degree days (DD) before 1 August are required to produce a 2nd generation of E. comes under climatic conditions in New York.Variationsin temperature-driven development are hypothesized to strongly influence year-to-year variability in leafhopper abundance. Degree-day accumulations by 30 June correlated well with degree-days by 1 August and may provide a useful early-warningsystem for predicting years in which leafhopper population densities are above averag

Modeling, Analysis and Simulation Approaches Used in Development of the National Aeronautics and Space Administration Max Launch Abort System

The National Aeronautics and Space Administration (NASA) Engineering and Safety Center was chartered to develop an alternate launch abort system (LAS) as risk mitigation for the Orion Project. Its successful flight test provided data for the design of future LAS vehicles. Design of the flight test vehicle (FTV) and pad abort trajectory relied heavily on modeling and simulation including computational fluid dynamics for vehicle aero modeling, 6-degree-of-freedom kinematics models for flight trajectory modeling, and 3-degree-of-freedom kinematics models for parachute force modeling. This paper highlights the simulation techniques and the interaction between the aerodynamics, flight mechanics, and aerodynamic decelerator disciplines during development of the Max Launch Abort System FTV

Phenology, Within-Vineyard Distribution, and Seasonal Movement of Eastern Grape Leafhopper (Homoptera: Cicadellidae) in New York Vineyards

Seasonal changes in within-vineyard distribution and abundance of Erythroneura comes (Say) adults and nymphs were investigated from 1989 to 1992. Trap catches of adults were highest in May and were concentrated in wooded areas next to vineyards. In 1989 and 1990 surveys, nymphal densities did not decline as distance from the vineyard edge increased. In 1991, however, nymphal densities were significantly higher at vineyard edges than in vineyard interiors in July, suggesting that oviposition initially was aggregated at vineyard edges. Subsequently, nymphal densities at vineyard edges and interiors were similar. Cumulative degree days (DD) for mean observation of first nymphs, first-generation, and second-generation peak populations, sampled at 14 vineyards in 1989, 1990, and 1991, were 390 ± 71, 648 ± 86, and 1,190 ± 154 DD (mean ± SD; base 10°C), respectively. Nymphal densities exceeded a provisional threshold of five per leaf in only 2, 25, 13, and 8% of vineyards untreated with insecticides in 1989, 1990, 1991, and 1992, respectively. These results show that leafhoppers do not cause economic injury in most New York vineyards in most years. Reduced insecticide strategies recently implemented for grape berry moth control will not greatly increase the need for insecticide applications directed at leafhoppers in New Yor

Young people's conceptualizations of the nature of cyberbullying: A systematic review and synthesis of qualitative research

© 2020 Elsevier Ltd Introduction: Cyberbullying is a serious public health problem facing young people. Adults do not have first-hand experience of being immersed in social media in their youth and this necessitates the inclusion of youth voice in efforts to understand and address cyberbullying. This study aimed to synthesize qualitative studies which had explored young people's conceptualizations of the nature of cyberbullying, with a view to informing conceptual and intervention development. Methods: A systematic review and meta-ethnographic synthesis of qualitative studies was conducted. Nine databases were searched from inception to July 2018. The Critical Appraisal Skills Program assessment tool was used to appraise the quality of included studies. Results: Of 4872 unique records identified, 79 were reviewed in detail and 13 studies comprising 753 young people from 12 countries were included. Five key concepts were identified: Intent, Repetition, Accessibility, Anonymity and Barriers to Disclosure. A “line of argument” illustrating young people's conceptualization of cyberbullying was developed. Conclusion: The significance of information and communication technology in young people's lives, and the complexity of the cyber world in which they connect, must be recognized in conceptualizations of cyebrbullying. The distinctive features of cyberbullying identified in young people's characterization can be used to inform bottom-up research and intervention efforts.This research was funded by the Health Research Board through the SPHeRE Programme SPHeRE/2013/

Bacteriophage Migration via Nematode Vectors: Host-Parasite-Consumer Interactions in Laboratory Microcosms

Pathogens vectored by nematodes pose serious agricultural, economic, and health threats; however, little is known of the ecological and evolutionary aspects of pathogen transmission by nematodes. Here we describe a novel model system with two trophic levels, bacteriophages and nematodes, each of which competes for bacteria. We demonstrate for the first time that nematodes are capable of transmitting phages between spatially distinct patches of bacteria. This model system has considerable advantages, including the ease of maintenance and manipulation at the laboratory bench, the ability to observe many generations in short periods, and the capacity to freeze evolved strains for later comparison to their ancestors. More generally, experimental studies of complex multispecies interactions, host-pathogen coevolution, disease dynamics, and the evolution of virulence may benefit from this model system because current models (e.g., chickens, mosquitoes, and malaria parasites) are costly to maintain, are difficult to manipulate, and require considerable space. Our initial explorations centered on independently assessing the impacts of nematode, bacterium, and phage population densities on virus migration between host patches. Our results indicated that virus transmission increases with worm density and host bacterial abundance; however, transmission decreases with initial phage abundance, perhaps because viruses eliminate available hosts before migration can occur. We discuss the microbial growth dynamics that underlie these results, suggest mechanistic explanations for nematode transmission of phages, and propose intriguing possibilities for future research

Exploring the roles of and interactions among microbes in dry co-digestion of food waste and pig manure using high-throughput 16S rRNA gene amplicon sequencing

peer-reviewedBackground With the increasing global population and increasing demand for food, the generation of food waste and animal manure increases. Anaerobic digestion is one of the best available technologies for food waste and pig manure management by producing methane-rich biogas. Dry co-digestion of food waste and pig manure can significantly reduce the reactor volume, capital cost, heating energy consumption and the cost of digestate liquid management. It is advantageous over mono-digestion of food waste or pig manure due to the balanced carbon/nitrogen ratio, high pH buffering capacity, and provision of trace elements. However, few studies have been carried out to study the roles of and interactions among microbes in dry anaerobic co-digestion systems. Therefore, this study aimed to assess the effects of different inocula (finished digestate and anaerobic sludge taken from wastewater treatment plants) and substrate compositions (food waste to pig manure ratios of 50:50 and 75:25 in terms of volatile solids) on the microbial community structure in food waste and pig manure dry co-digestion systems, and to examine the possible roles of the previously poorly described bacteria and the interactions among dry co-digestion-associated microbes. Results The dry co-digestion experiment lasted for 120 days. The microbial profile during different anaerobic digestion stages was explored using high-throughput 16S rRNA gene amplicon sequencing. It was found that the inoculum factor was more significant in determining the microbial community structure than the substrate composition factor. Significant correlation was observed between the relative abundance of specific microbial taxa and digesters’ physicochemical parameters. Hydrogenotrophic methanogens dominated in dry co-digestion systems. Conclusions The possible roles of specific microbial taxa were explored by correlation analysis, which were consistent with the literature. Based on this, the anaerobic digestion-associated roles of 11 bacteria, which were previously poorly understood, were estimated here for the first time. The inoculum played a more important role in determining the microbial community structure than substrate composition in dry co-digestion systems. Hydrogenotrophic methanogenesis was a significant methane production pathway in dry co-digestion systems

Future-Proofing Your Microbiology Resource Announcements Genome Assembly for Reproducibility and Clarity.

Descriptions of resources, like the genome assemblies reported in Microbiology Resource Announcements, are often frozen at their time of publication, yet they will need to be interpreted in the midst of continually evolving technologies. It is therefore important to ensure that researchers accessing published resources have access to all of the information required to repeat, interpret, and extend these original analyses. Here, we provide a set of suggestions to help make certain that published resources remain useful and repeatable for the foreseeable future

Activation of the innate immune receptor Dectin-1 upon formation of a 'phagocytic synapse'.

Innate immune cells must be able to distinguish between direct binding to microbes and detection of components shed from the surface of microbes located at a distance. Dectin-1 (also known as CLEC7A) is a pattern-recognition receptor expressed by myeloid phagocytes (macrophages, dendritic cells and neutrophils) that detects β-glucans in fungal cell walls and triggers direct cellular antimicrobial activity, including phagocytosis and production of reactive oxygen species (ROS). In contrast to inflammatory responses stimulated upon detection of soluble ligands by other pattern-recognition receptors, such as Toll-like receptors (TLRs), these responses are only useful when a cell comes into direct contact with a microbe and must not be spuriously activated by soluble stimuli. In this study we show that, despite its ability to bind both soluble and particulate β-glucan polymers, Dectin-1 signalling is only activated by particulate β-glucans, which cluster the receptor in synapse-like structures from which regulatory tyrosine phosphatases CD45 and CD148 (also known as PTPRC and PTPRJ, respectively) are excluded (Supplementary Fig. 1). The 'phagocytic synapse' now provides a model mechanism by which innate immune receptors can distinguish direct microbial contact from detection of microbes at a distance, thereby initiating direct cellular antimicrobial responses only when they are required

Predominance of Rotavirus P[4]G2 in a Vaccinated Population, Brazil

We identified 21 rotaviruses in 129 patients with diarrhea in a Brazilian city with high rotavirus vaccine coverage. All rotaviruses were genotype P[4]G2 with 1 mixed infection with P[NT]G9. Although virus predominance could have occurred randomly, the vaccine may be less protective against P[4]G2. Prospective surveillance is urgently needed