Factibilidad para la creación de un restaurante que ofrezca entretenimiento familiar

Con el presente trabajo se pretendió analizar la factibilidad de crear un restaurante con entretenimiento familiar para familias de estratos 5 y 6 de la ciudad de Medellín y su área metropolitana, al tener en cuenta el análisis del entorno macroeconómico del sector y la factibilidad específica por áreas, comercial, técnica, legal, ambiental y financiera. Se utilizaron diferentes estrategias de mercadeo que permitieran aproximarse a una información fidedigna del sector, como las fuentes primarias: encuentros conversacionales, encuestas y observación, y las secundarias, como diversas lecturas a través de páginas web, de las que se obtuvo, como resultado, la constatación de las necesidades insatisfechas en el grupo de personas objetivo y de la poca competencia específica en el segmento, lo que condujo, además, a un estudio de factibilidad en el que se documentó la viabilidad del estudio para establecer un plan de ejecución del proyecto.The present work intends to analyze the feasibility of creating a restaurant with family entertainment for stratum families 5-6 of the Medellin and the metropolitan area, taking into account the analysis of the macroeconomic environment of the sector and the specific feasibility by areas, commercial, technical, Legal, environmental and financial. Different marketing strategies were used to allow us to approach reliable information on the sector as the primary sources: interviews, surveys, observation and secondary sources as diverse readings. Obtaining, as a result, unmet needs in the group of target people, also little specific competition in this segment, generating additionally a feasibility study where the viability of the study is documented to establish an execution plan for this project

Diseño de una aplicación docente para el control del equipamiento del laboratorio: PC-LAB.

En la actual formación universitaria, se reflexiona sobre los métodos usados en la enseñanza, así como en el tiempo empleado por los alumnos en asimilar los conocimientos ligados a cada materia. Ambos aspectos, se reflejan en la elaboración de la guía particular de cada asignatura. Esta visión hace que el método de enseñanza tradicional de las materias, utilizando clases magistrales, sólo es una parte en la cadena del aprendizaje. Con el ánimo de contribuir a esa nueva perspectiva, se ha creado el trabajo que a continuación se presenta. Un instrumento virtual, desarrollado con el software LabVIEW® (Laboratory Virtual Engineering workbench), que permite la conexión entre el ordenador y el puesto del laboratorio. Con esta aplicación, se quiere favorecer el grado de implicación por parte del alumno en el proceso de enseñanza – aprendizaje y proporcionar herramientas con las que los alumnos puedan construir su conocimiento

Small molecules targeting glycogen synthase kinase 3 as potential drug candidates for the treatment of retinitis pigmentosa

6 p.-5 fig.Retinitis pigmentosa (RP) is an inherited retinal dystrophy that courses with progressive degeneration of retinal tissue and loss of vision. Currently, RP is an unpreventable, incurable condition. We propose glycogen synthase kinase 3 (GSK-3) inhibitors as potential leads for retinal cell neuroprotection, since the retina is also a part of the central nervous system and GSK-3 inhibitors are potent neuroprotectant agents. Using a chemical genetic approach, diverse small molecules with different potency and binding mode to GSK-3 have been used to validate and confirm GSK-3 as a pharmacological target for RP. Moreover, this medicinal chemistry approach has provided new leads for the future disease-modifying treatment of RP.This work has been partially funded by the Spanish MINECO grants (SAF2012-37979-C03-01 to AM and SAF2013-41059-R to EJdlR).Peer reviewe

Genomic Features for Desiccation Tolerance and Sugar Biosynthesis in the Extremophile Gloeocapsopsis sp. UTEX B3054

For tolerating extreme desiccation, cyanobacteria are known to produce both compatible solutes at intracellular level and a copious amount of exopolysaccharides as a protective coat. However, these molecules make cyanobacterial cells refractory to a broad spectrum of cell disruption methods, hindering genome sequencing, and molecular studies. In fact, few genomes are already available from cyanobacteria from extremely desiccated environments such as deserts. In this work, we report the 5.4 Mbp draft genome (with 100% of completeness in 105 contigs) of Gloeocapsopsis sp. UTEX B3054 (subsection I; Order Chroococcales), a cultivable sugar-rich and hardly breakable hypolithic cyanobacterium from the Atacama Desert. Our in silico analyses focused on genomic features related to sugar-biosynthesis and adaptation to dryness. Among other findings, screening of Gloeocapsopsis genome revealed a unique genetic potential related to the biosynthesis and regulation of compatible solutes and polysaccharides. For instance, our findings showed for the first time a novel genomic arrangement exclusive of Chroococcaceae cyanobacteria associated with the recycling of trehalose, a compatible solute involved in desiccation tolerance. Additionally, we performed a comparative genome survey and analyses to entirely predict the highly diverse pool of glycosyltransferases enzymes, key players in polysaccharide biosynthesis and the formation of a protective coat to dryness. We expect that this work will set the fundamental genomic framework for further research on microbial tolerance to desiccation and to a wide range of other extreme environmental conditions. The study of microorganisms like Gloeocapsopsis sp. UTEX B3054 will contribute to expand our limited understanding regarding water optimization and molecular mechanisms allowing extremophiles to thrive in xeric environments such as the Atacama Desert

Glutathione peroxidase contributes with heme oxygenase-1 to redox balance in mouse brain during the course of cerebral malaria

Oxidative stress has been attributed both a key pathogenic and rescuing role in cerebral malaria (CM). In a Plasmodium berghei ANKA murine model of CM, host redox signaling and functioning were examined during the course of neurological damage. Host antioxidant defenses were early altered at the transcriptional level indicated by the gradually diminished expression of superoxide dismutase-1 (sod-1), sod-2, sod-3 and catalase genes. During severe disease, this led to the dysfunctional activity of superoxide dismutase and catalase enzymes in damaged brain regions. Vitagene associated markers (heat shock protein 70 and thioredoxin-1) also showed a decaying expression pattern that paralleled reduced expression of the transcription factors Parkinson disease 7, Forkhead box O 3 and X-box binding protein 1 with a role in preserving brain redox status. However, the oxidative stress markers reactive oxygen/nitrogen species were not accumulated in the brains of CM mice and redox proteomics and immunohistochemistry failed to detect quantitative or qualitative differences in protein carbonylation. Thus, the loss of antioxidant capacity was compensated for in all cerebral regions by progressive upregulation of heme oxygenase-1, and in specific regions by early glutathione peroxidase-1 induction. This study shows for the first time a scenario of cooperative glutathione peroxidase and heme oxygenase-1 upregulation to suppress superoxide dismutase, catalase, heat shock protein-70 and thioredoxin-1 downregulation effects in experimental CM, counteracting oxidative damage and maintaining redox equilibrium. Our findings reconcile the apparent inconsistency between the lack of oxidative metabolite build up and reported protective effect of antioxidant therapy against CM.Ministerio de Educación y Ciencia (España)Universidad Complutense de MadridDepto. de Bioquímica y Biología MolecularFac. de FarmaciaTRUEpu

Glutathione peroxidase contributes with heme oxygenase-1 to redox balance in mouse brain during the course of cerebral malaria

Oxidative stress has been attributed both a key pathogenic and rescuing role in cerebral malaria (CM). In a Plasmodium berghei ANKA murine model of CM, host redox signaling and functioning were examined during the course of neurological damage. Host antioxidant defenses were early altered at the transcriptional level indicated by the gradually diminished expression of superoxide dismutase-1 (sod-1), sod-2, sod-3 and catalase genes. During severe disease, this led to the dysfunctional activity of superoxide dismutase and catalase enzymes in damaged brain regions. Vitagene associated markers (heat shock protein 70 and thioredoxin-1) also showed a decaying expression pattern that paralleled reduced expression of the transcription factors Parkinson disease 7, Forkhead box O 3 and X-box binding protein 1 with a role in preserving brain redox status. However, the oxidative stress markers reactive oxygen/nitrogen species were not accumulated in the brains of CM mice and redox proteomics and immunohistochemistry failed to detect quantitative or qualitative differences in protein carbonylation. Thus, the loss of antioxidant capacity was compensated for in all cerebral regions by progressive upregulation of heme oxygenase-1, and in specific regions by early glutathione peroxidase-1 induction. This study shows for the first time a scenario of cooperative glutathione peroxidase and heme oxygenase-1 upregulation to suppress superoxide dismutase, catalase, heat shock protein-70 and thioredoxin-1 downregulation effects in experimental CM, counteracting oxidative damage and maintaining redox equilibrium. Our findings reconcile the apparent inconsistency between the lack of oxidative metabolite build up and reported protective effect of antioxidant therapy against CM.Ministerio de Educación y Ciencia (España)Universidad Complutense de MadridDepto. de Bioquímica y Biología MolecularFac. de FarmaciaTRUEpu

A GSK-3b modulator delays photoreceptor cell death and preserves visual function in the rd10 mouse model of retinitis pigmentosa

1 p.Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal dystrophies that lead to blindness. Photoreceptor cell death, reactive gliosis and retinal inammation are common features in animal models of the disease. The enzyme Glycogen Synthase Kinase-3 Beta (GSK-3b) is involved in inammatory processes associated to diverse neurodegenerative pathologies. The aim of our study is to test in the rd10 mouse whether the GSK-3b inhibitor VP3.15 (a small heterocyclic molecule) is a potential therapeutic treatment for RP.SAF2013-41059-RPeer reviewe

Acute Impacts of Different Types of Exercise on Circulating α-Klotho Protein Levels

[EN] Elevated plasma α-klotho (αKl) protects against several ageing phenotypes and has been proposed as a biomarker of a good prognosis for different diseases. The beneficial health effects of elevated plasma levels of soluble αKl (SαKl) have been likened to the positive effects of exercise on ageing and chronic disease progression. It has also been established that molecular responses and adaptations differ according to exercise dose. The aim of this study is to compare the acute SαKl response to different exercise interventions, cardiorespiratory, and strength exercise in healthy, physically active men and to examine the behavior of SαKl 72h after acute strength exercise.S

Specific bacteriophages against Enterococcus faecalis in root canal infection : a scoping review

Antecedentes: Existen varios factores que conducen al fracaso del tratamiento endodóntico, sin embargo, el factor más asociado a esta situación es la persistencia de microorganismos dentro del sistema de conductos radiculares. Enterococcus faecalis es el microorganismo que más se ha asociado al fracaso endodóntico por ser multirresistente. Se requiere una nueva alternativa que elimine este microorganismo. Los bacteriófagos son virus capaces de destruir células bacterianas, por lo tanto, es necesario investigar más sobre el tema y la aplicación clínica en endodoncia de la fagoterapia. Objetivo: El propósito de este artículo es revisar la evidencia disponible asociada a la actividad lítica de bacteriófagos específicos contra E. faecalis aplicada en odontología a través de los años. Materiales y métodos: Se realizó un estudio de revisión de la literatura científica mediante la metodología de Scoping Review. Para ello fueron utilizadas cuatro bases de datos biomédicas: MEDLINE Pubmed, Clinical Key, Science Direct y Scielo. Resultados: Posterior a la búsqueda en las 4 bases de datos y luego de hacer la eliminación de aquellas referencias duplicadas, que no tuvieran relación con odontología o sin disponibilidad de acceso al texto completo, se seleccionaron en total 13 referencias para realizar este Scoping Review. Conclusión: La literatura disponible en las bases de datos es muy limitada actualmente, se requiere mayor investigación de este tema específicamente relacionada con las infecciones endodónticas y la aplicación clínica de la terapia con fagos.Background: There are several factors that lead to endodontic treatment failure, however, the factor most associated with this situation is the persistence of microorganisms within the root canal system. Enterococcus faecalis is the microorganism that has been most associated with endodontic failure. A new alternative is required to eliminate this microorganism. Bacteriophages are viruses capable of destroying bacterial cells, therefore, more research is needed on the subject and the clinical application of phage therapy in endodontics. Objective: The purpose of this article is to review the available evidence associated with the lytic activity of specific bacteriophages against E. faecalis applied in dentistry over the years. Materials and methods: A review study of the scientific literature was carried out using the Scoping Review methodology. Four biomedical databases were used for this: MEDLINE Pubmed, Clinical Key, Science Direct and Scielo. Results: After the search in the 4 databases and after eliminating those duplicate references that were not related to dentistry or without availability of access to the full text, a total of 13 references were selected to carry out this Scoping Review. Conclusion: The literature available in the databases is currently very limited, more research is required on this topic specifically related to endodontic infections and the clinical application of phage therapy.Especialista en EndodonciaEspecializació

Prevalence of acromegaly in patients with symptoms of sleep apnea

Acromegaly is a rare disease with nonspecific symptoms with acral enlargement being almost universally present at diagnosis. The estimated prevalence is 40–125 cases/million but targeted universal screening studies have found a higher prevalence (about 10 fold). The aim of the ACROSAHS study was to investigate the prevalence of acromegaly and acromegaly comorbidities in patients with sleep apnea symptoms and acral enlargement. ACROSAHS was a Spanish prospective non-interventional epidemiological study in 13 Hospital sleep referral units. Facial and acral enlargement symptoms including: ring size and shoe size increase, tongue, lips and jaws enlargement, paresthesia or carpal tunnel syndrome and widening of tooth spaces, as well as other typical acromegaly comorbidities were recorded with a self-administered questionnaire of patients who attended a first visit for sleep apnea symptoms between 09/2013 and 07/2014. Serum insulin-like growth factor type 1 (IGF1) was measured in patients with ≥1 acral symptom to determine the prevalence of acromegaly. Of the 1557 patients enrolled, 1477 with complete data (72% male) were analyzed. 530 patients (36%) reported at least 1 acral enlargement symptom and were tested for IGF-1, 41 were above range, persisted in 7, and among those, 2 cases of acromegaly were diagnosed (prevalence of at least 1.35 cases/1000). Overall, 1019 patients (69%) had ≥2 acromegaly symptoms and should have been screened according to guidelines; moreover 373 patients (25%) had ≥1 symptom of acral enlargement plus ≥3 other acromegaly symptoms. In conclusion, in patients with sleep apnea symptoms and acral enlargement, we found an acromegaly prevalence of at least 1.35 cases per 1000 and a high prevalence of typical acromegaly symptoms. It is important that sleep specialists are aware of acromegaly symptoms to aid with acromegaly diagnosis.The study was investigator initiated and was partially sponsored by Ipsen Pharma, S.A., Spai