The Diffraction Model and its Applicability for Wakefield Calculations

The operation of a Free Electron Laser (FEL) in the ultraviolet or in the X-ray regime requires the acceleration of electron bunches with an rms length of 25 to 50 micro meters. The wakefields generated by these sub picosecond bunches extend into the frequency range well beyond the threshold for Cooper pair breakup (about 750 GHz) in superconducting niobium at 2 K. It is shown, that the superconducting cavities can indeed be operated with 25 micro meter bunches without suffering a breakdown of superconductivity (quench), however at the price of a reduced quality factor and an increased heat transfer to the superfluid helium bath. This was first shown by wakefield calculations based on the diffraction model. In the meantime a more conventional method of computing wake fields in the time domain by numerical methods was developed and used for the wakefield calculations. Both methods lead to comparable results: the operation of TESLA with 25 micro meter bunches is possible but leads to an additional heat load due to the higher order modes (HOMs). Therefore HOM dampers for these high frequencies are under construction. These dampers are located in the beam pipes between the 9-cell cavities. So it is of interest, if there are trapped modes in the cavity due to closed photon orbits. In this paper we investigate the existence of trapped modes and the distribution of heat load over the surface of the TESLA cavity by numerical photon tracking.Comment: Linac2000 conference paper ID No. MOE0

Spectral functions from the real-time functional renormalization group

We employ the functional renormalization group approach formulated on the Schwinger-Keldysh contour to calculate real-time correlation functions in scalar field theories. We provide a detailed description of the formalism, discuss suitable truncation schemes for real-time calculations as well as the numerical procedure to self-consistently solve the flow equations for the spectral function. Subsequently, we discuss the relations to other perturbative and non-perturbative approaches to calculate spectral functions, and present a detailed comparison and benchmark in $d=0+1$ dimensions.Comment: 40 pages + 16 pages appendix, 8 figure

Drebrin is a novel connexin-43 binding partner that links gap junctions to the submembrane cytoskeleton

AbstractBackground: Connexins form gap junctions that mediate the transfer of ions, metabolites, and second messengers between contacting cells. Many aspects of connexin function, for example cellular transport, plaque assembly and stability, and channel conductivity, are finely tuned and likely involve proteins that bind to connexins' cytoplasmic domains. However, little is known about such regulatory proteins. To identify novel proteins that interact with the COOH-terminal domain of Connexin-43 (Cx43), the most widely expressed connexin family member, we applied a proteomics approach to screen fractions of mouse tissue homogenates for binding partners.Results: Drebrin was recovered as a binding partner of the Cx43 COOH-terminal domain from mouse brain homogenate. Drebrin had previously been described as an actin binding protein that diminishes in brains during Alzheimer's disease. The novel Drebrin-Cx43 interaction identified by proteomics was confirmed by colocalization of endogenous proteins in astrocytes and Vero cells, coimmunoprecipitation, electron microscopy, electrophysiology, coexpression of both proteins with fluorescent tags, and live-cell FRET analysis. Depletion of Drebrin in cells with siRNA results in impaired cell-cell coupling, internalization of gap junctions, and targeting of Cx43 to a degradative pathway.Conclusions: We conclude that Drebrin is required for maintaining Cx43-containing gap junctions in their functional state at the plasma membrane. It is thus possible that Drebrin may interact with gap junctions in zones of cell-cell contacts in a regulated fashion in response to extracellular signals. The rearrangement or disruption of interactions between connexins and the Drebrin-containing submembrane cytoskeleton directs connexins to degradative cellular pathways

Non-Invasive Optical Motion Tracking Allows Monitoring of Respiratory Dynamics in Dystrophin-Deficient Mice

Duchenne muscular dystrophy (DMD) is the most common x-chromosomal inherited dystrophinopathy which leads to progressive muscle weakness and a premature death due to cardiorespiratory dysfunction. The mdx mouse lacks functional dystrophin protein and has a comparatively human-like diaphragm phenotype. To date, diaphragm function can only be inadequately mapped in preclinical studies and a simple reliable translatable method of tracking the severity of the disease still lacks. We aimed to establish a sensitive, reliable, harmless and easy way to assess the effects of respiratory muscle weakness and subsequent irregularity in breathing pattern. Optical respiratory dynamics tracking (ORDT) was developed utilising a camera to track the movement of paper markers placed on the thoracic-abdominal region of the mouse. ORDT successfully distinguished diseased mdx phenotype from healthy controls by measuring significantly higher expiration constants (k) in mdx mice compared to wildtype (wt), which were also observed in the established X-ray based lung function (XLF). In contrast to XLF, with ORDT we were able to distinguish distinct fast and slow expiratory phases. In mdx mice, a larger part of the expiratory marker displacement was achieved in this initial fast phase as compared to wt mice. This phenomenon could not be observed in the XLF measurements. We further validated the simplicity and reliability of our approach by demonstrating that it can be performed using free-hand smartphone acquisition. We conclude that ORDT has a great preclinical potential to monitor DMD and other neuromuscular diseases based on changes in the breathing patterns with the future possibility to track therapy response

Mesoderm migration in Drosophila is a multi-step process requiring FGF signaling and integrin activity

Migration is a complex, dynamic process that has largely been studied using qualitative or static approaches. As technology has improved, we can now take quantitative approaches towards understanding cell migration using in vivo imaging and tracking analyses. In this manner, we have established a four-step model of mesoderm migration during Drosophila gastrulation: (I) mesodermal tube formation, (II) collapse of the mesoderm, (III) dorsal migration and spreading and (IV) monolayer formation. Our data provide evidence that these steps are temporally distinct and that each might require different chemical inputs. To support this, we analyzed the role of fibroblast growth factor (FGF) signaling, in particular the function of two Drosophila FGF ligands, Pyramus and Thisbe, during mesoderm migration. We determined that FGF signaling through both ligands controls movements in the radial direction. Thisbe is required for the initial collapse of the mesoderm onto the ectoderm, whereas both Pyramus and Thisbe are required for monolayer formation. In addition, we uncovered that the GTPase Rap1 regulates radial movement of cells and localization of the beta-integrin subunit, Myospheroid, which is also required for monolayer formation. Our analyses suggest that distinct signals influence particular movements, as we found that FGF signaling is involved in controlling collapse and monolayer formation but not dorsal movement, whereas integrins are required to support monolayer formation only and not earlier movements. Our work demonstrates that complex cell migration is not necessarily a fluid process, but suggests instead that different types of movements are directed by distinct inputs in a stepwise manner

UMA PROPOSTA DIDÁTICA CONTEXTUALIZADA PARA O CURSO DE LICENCIATURA EM QUÍMICA: O CASO DO ENSINO DE QUÍMICA ANALÍTICA

Este trabalho apresenta uma proposta didática desenvolvida numa disciplina de Química Analítica do curso de Licenciatura em Química – UDESC. A proposta contém atividades embasadas em metodologia ativa, valorizando de forma integrada a formação científica e a formação para atuação na sociedade. Os estudantes trabalharam em grupo e tiveram como problema evidenciar e quantificar a migração de ésteres de ftalato (EF) de amostras cotidianas de embalagens para a alimentação humana utilizando uma técnica analítica de cromatografia a gás. A atividade propiciou desenvolver habilidades requeridas para operação de um equipamento de análise (CG-EM), desenvolver e aplicar conhecimentos químicos e vivenciar atividades próprias da ciência química tanto do ponto de vista científico como do social.Palavras chave: Formação de Professores; Contextualização; Metodologia Ativa.

A suicide gene approach using the human pro-apoptotic protein tBid inhibits HIV-1 replication

<p>Abstract</p> <p>Background</p> <p>Regulated expression of suicide genes is a powerful tool to eliminate specific subsets of cells and will find widespread usage in both basic and applied science. A promising example is the specific elimination of human immunodeficiency virus type 1 (HIV-1) infected cells by LTR-driven suicide genes. The success of this approach, however, depends on a fast and effective suicide gene, which is expressed exclusively in HIV-1 infected cells. These preconditions have not yet been completely fulfilled and, thus, success of suicide approaches has been limited so far. We tested truncated Bid (tBid), a human pro-apoptotic protein that induces apoptosis very rapidly and efficiently, as suicide gene for gene therapy against HIV-1 infection.</p> <p>Results</p> <p>When tBid was introduced into the HIV-1 LTR-based, Tat- and Rev-dependent transgene expression vector pLRed(INS)₂R, very efficient induction of apoptosis was observed within 24 hours, but only in the presence of both HIV-1 regulatory proteins Tat and Rev. Induction of apoptosis was not observed in their absence. Cells containing this vector rapidly died when transfected with plasmids containing full-length viral genomic DNA, completely eliminating the chance for HIV-1 replication. Viral replication was also strongly reduced when cells were infected with HIV-1 particles.</p> <p>Conclusions</p> <p>This suicide vector has the potential to establish a safe and effective gene therapy approach to exclusively eliminate HIV-1 infected cells before infectious virus particles are released.</p