Press Bulletin, Vol. V, No. 6, 1942

Poston newspaper covers health issues, property problems, tofu equipment, social gatherings and parties, sports results, production of camouflage nets, ace club cabinet members, nursery department reports of flowers for different purposes, police outfits, wedding announcements, birth notices, smallpox, work meetings, part-time workers, school announcements, club meetings, diphtheria, girls judo classes, queen contest electio

Press Bulletin, Vol. VII, No. 10, 1942

Poston Relocation Center newspaper covers settled negotiations, new press machine, allowances, special holiday magazine, an editorial, announcements, statements, weather, social events, class registrations, census data, new and remodeled spaces, birth notices, first aid classes, religious services, library donations, sports score results cancelled sports classes, and social tournament

Press Bulletin, Vol. V, No. 3, 1942

Poston Relocation Center newspaper. Covers basic policy for Poston relocation center, teachers attending monthly sessions, the Press looking for delivery kids, Dr. Kita elected head delegate, education, new Caucasian teachers, book donations, book loan, temporary renovations, The Value of Art, Red Cross safety courses, recreational and social activities, home grown vegetables, Red Cross Inauguration, library attendance, shoe repair shop, softball results, and information on Queen contest

Press Bulletin, Vol. VI, No. 22, 1942

Poston Relocation Center newspaper covers low price goods, employment situation, city council, social events, social classes, an editorial, education, elections,Japanese cemetery, meeting schedule, High school athletic competition, children\u27s playground, Library donations, garden vegetables, typing class, school PE, night classes, church notices, Industrial department meeting, and sport score results

Rekayasa Bakteri Untuk Ternak Dan Manusia: Pembuatan Mutan Escherichia Coli Penghasil Protein Rekombinan

Protein rekombinan seperti vaksin, antibodi, hormon, dan obat-obatan, semakin dibutuhkan oleh ternak dan manusia. Hambatan utama untuk menghasilkan protein rekombinan pada Escherichia coli sebagai inang yang digunakan paling luas adalah degradasi oleh enzim proteolitik. Hal ini disebabkan karena E. coli memiliki sejumlah enzim proteolitik yang tersebar di dalam sitoplasmanya. Untuk itu, lebih dari 90% degradasi protein terjadi di dalam sitoplasmanya. Pada penelitian ini, peneliti telah menghasilkan mutant E. coli BW25113 yang tidak memiliki gen penyandi enzim protease dengan menggunakan kombinasi metode pengerusakan kromosom dan metode transduksi phage P1. Pembuatan mutan tersebut dimulai dengan pengerusakan gen penyandi enzim protease pada kromosom bakteri dengan produk PCR yang memiliki bagian yang homolog dengan gen target. Mutan-mutan yang dihasilkan kemudian digunakan untuk menghasilkan mutan ganda dengan metode Transduksi phage P1. Analisis fenotif dan genotif menunjukkan bahwa kombinasi kedua metode tersebut sangat efektif untuk membuat lebih dari satu mutasi pada E. coli. Untuk itu, mutan E. coli yang telah diperoleh akan sangat bermanfaat untuk menghasilkan aneka protein rekombinan untuk ternak dan manusia

Whole-body MRI of patients with polymyalgia rheumatica identifies a distinct subset with complete patient-reported response to glucocorticoids

Objectives: To determine whether whole-body MRI defines clinically-relevant subgroups within polymyalgia rheumatica (PMR) including glucocorticoid responsiveness. Methods: 22 patients with PMR and 16 with rheumatoid arthritis, untreated and diagnosed by consultant rheumatologists, underwent whole-body, multiple-joint MRI, scored by two experts. PMR patients reported whether they felt “back to normal” on glucocorticoid therapy and were followed for a median of 2 years. Results: All PMR patients were deemed to respond to glucocorticoids clinically. A characteristic pattern of symmetrical, extracapsular inflammation, adjacent to greater trochanter, acetabulum, ischial tuberosity and/or symphysis pubis, was observed in 14/22 of the PMR cases. In PMR, this pattern was associated with complete glucocorticoid response (p=0.01), higher pre-treatment C-reactive protein (CRP) and serum IL-6, and better post-treatment fatigue and function. Only 1/14 in the extracapsular group could stop glucocorticoids within 1 year, compared to 4/7 of the others. A score derived from the five sites discriminating best between PMR and RA correlated with IL-6 (p<0.002). IL-6 levels ≥16.8 pg/mL had 86% sensitivity and 86% specificity for the extracapsular MRI pattern. Conclusions: A subset of patients with rheumatologist-diagnosed PMR had a characteristic, extracapsular pattern of MRI inflammation, associated with elevated IL-6/CRP and with complete patient-reported glucocorticoid responsiveness

‘Priming’ exercise and O2 uptake kinetics during treadmill running

We tested the hypothesis that priming exercise would speed kinetics during treadmill running. Eight subjects completed a square-wave protocol, involving two bouts of treadmill running at 70% of the difference between the running speeds at lactate threshold (LT) and max, separated by 6-min of walking at 4 km h−1, on two occasions. Oxygen uptake was measured breath-by-breath and subsequently modelled using non-linear regression techniques. Heart rate and blood lactate concentration were significantly elevated prior to the second exercise bout compared to the first. However, kinetics was not significantly different between the first and second exercise bouts (mean ± S.D., phase II time constant, Bout 1: 16 ± 3 s vs. Bout 2: 16 ± 4 s; slow component amplitude, Bout 1: 0.24 ± 0.10 L min−1vs. Bout 2: 0.20 ± 0.12 L min−1; mean response time, Bout 1: 34 ± 4 s vs. Bout 2: 34 ± 6 s; P > 0.05 for all comparisons). These results indicate that, contrary to previous findings with other exercise modalities, priming exercise does not alter kinetics during high-intensity treadmill running, at least in physically active young subjects. We speculate that the relatively fast kinetics and the relatively small slow component in the control (‘un-primed’) condition negated any enhancement of kinetics by priming exercise in this exercise modality

CD4+ T-cell-dependent differentiation of CD23+ follicular B cells contributes to the pulmonary pathology in a primary Sjögren’s syndrome mouse model

Introduction: Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease that affects the function of exocrine glands, such as the lacrimal and the salivary glands. Extraglandular lesions and malignant lymphoma also occur during the progressive stage of pSS. We have, herein, focused on the pulmonary lesions of pSS and have aimed clarifying their pathophysiological mechanism by comparing the glandular with the extraglandular lesions observed in a mouse model of pSS. Results: The histopathological analysis of lung tissues obtained from NFS/sld mice that have undergone neonatal thymectomy was performed. Moreover, in vivo and in vitro experiments were conducted along with immunological analyses in order to characterize the unique phenotypes of the pulmonary lesions identified in these pSS model mice. Inflammatory lesions with a bronchus-associated lymphoid tissue-like structure were identified in the lungs of pSS model mice. In addition, relative to salivary gland lesions, pulmonary lesions showed increased CD23+ follicular B (FB) cells. In vitro and pulmonary B cells were more readily driven to CD23+ FB cell phenotype than salivary gland B cells in pSS model mice. Furthermore, the CD23+ FB cell differentiation was found to be enhanced in a CD4+ T-cell-dependent manner under a Th2-type condition in the lungs of herein examined pSS model mice. Discussion: A Th2-type response in the pSS lung may promote the progression of autoimmune lesions through an enhanced abnormal differentiation of B cells

Successful pacing profiles of Olympic and IAAF World Championship middle-distance runners across qualifying rounds and finals

Purpose: This was the first study to analyze high-resolution pacing data from multiple global championships, allowing for deeper and rigorous analysis of pacing and tactical profiles in elite-standard middle-distance racing. The aim of this study was to analyze successful and unsuccessful middle-distance pacing profiles and variability across qualifying rounds and finals. Methods: Finishing and 100-m split speeds and season’s best times (SB) were collected for 265 men and 218 women competing in 800 m and 1500 m races, with pace variability expressed using coefficient of variation (CV). Results: In both events, successful athletes generally separated themselves from slower athletes in the final 200 m, not by speeding up, but by avoiding slowing compared with competitors. This was despite different pacing profiles between events in the earlier part of the race preceding the endspurt. Approximately 10% of athletes ran SBs, showing a tactical approach to elite-standard middle-distance racing, and possible fatigue across rounds. Men’s and women’s pacing profiles were remarkably similar within each event, but the previously undescribed seahorse-shaped profile in the 800 m (predominantly positive pacing) differed from the J-shaped negative pacing of the 1500 m. Pacing variability was high compared with world records, especially in the finals (CV: 5.2 – 9.1%), showing that athletes need to be able to vary pace and cope with surges. Conclusions: Previous studies have focussed more on athletes in finals, but the present study showed that the best athletes had the physiological capacity to vary pace and respond to surges through successive competition rounds