Возможность визуализации распределения в организме человека аутологичных мезенхимальных клеток, введенных в артериальное русло печени

Objective: to visualize the cellular structures entered into the arterial course of a liver, for the purpose of assessment of a possibility of their fixing in liver tissue.Materials and methods. Introduction of mesenchymal stem autologichny cells, marked by iron oxide nanoparticles was carried out to 10 patients with the diagnosis cirrhosis. Introduction was carried out to the arterial course of a liver.Results. The mesenchymal autologichny cages entered into the arterial course of a liver, marked iron oxide nanoparticles, are visualized in tissue of a liver of patients by means of a magnetic and resonant tomography.Conclusion. Mesenchymal autologichny cages, marked iron oxide nanoparticles, it is possible to visualize in a human body by means of a magnetic and resonant tomography. The mesenchymal stem autologichny cells entered into the arterial course of a liver are fixed in liver tissue.Цель исследования: визуализировать вводимые в артериальное русло печени клеточные структуры с целью оценки возможности их фиксации в ткани печени.Материал и методы. Введение мезенхимальных стволовых аутологичных клеток, меченных наночастицами оксида железа, провели 10 пациентам с диагнозом цирроз печени. Введение выполняли в артериальное русло печени.Результаты. Введенные в артериальное русло печени мезенхимальные аутологичные клетки, меченные наночастицами оксида железа, визуализированы в ткани печени пациентов при помощи магнитно-резонансной томографии.Заключение. Мезенхимальные аутологичные клетки, меченные наночастицами оксида железа, возможно визуализировать в организме человека при помощи магнитно-резонансной томографии. Вводимые в артериальное русло печени мезенхимальные стволовые аутологичные клетки фиксируются в ткани печени

Influence of “Chelavite” Mineral Supplement Use on Cadmium and Lead Content in Blood, Wool and Milk of Heavy Cows

The purpose of this research was to study the influence of this supplement use on the concentration of cadmium and lead in blood serum, wool and milk of heavy cows. The cows of milking herd of black-and-white breed, of 3-5 years old were the object of research. Their yearly milk production was 6 thousand litters (control and experimental group, each having 15 heads). The cows have been fed according to the balanced ration for heavy cows. The mineral supplement dose was determined according to the instruction for application for cows of experimental group with feed. The curative dose was 0.6 ml for 10 kg of body mass 1 time a day during 30 days. The samples were taken before giving the mineral supplement “Chelavite” and after the course had finished. The device Unicam AAS-939 was used to determine the cadmium and lead content in blood, wool and milk by way of atomic absorption spectrophotometry. It has been found that the cadmium level reduced by 2.35 times, the lead level reduced by 1,5 times in cows blood, the cadmium level reduced by 1.33 times, the lead level reduced by 4.34 times in cows wool, the cadmium level reduced by 2.2 times, the lead level reduced by 3.7 times in cows milk after giving them mineral supplement “Chelavite”. Thus, the application of chelate compounds in form of “Chelavite” for cows reduces concentration of heavy metals such as cadmium and lead. Then this is one of the ways to improve the milk quality

МЕЗЕНХИМАЛЬНЫЕ СТРОМАЛЬНЫЕ КЛЕТКИ - ВОЗМОЖНОСТЬ ВОССТАНОВИТЬ МИОМЕТРИЙ

The scar on the uterus is one of the most urgent problems of modern obstetrics, having a negative impact on the reproductive function at all stages of its implementation. One of the promising areas of fundamental sciences of biomedical profile is the use of stem cells to treat various conditions. The article presents the data of an experimental study describing the tissue effects of the introduction of a culture of mesenchymal stem cells in the scar area on the uterus after cesarean section. The positive effect of mesenchymal stem cells has been proved, which contributes to the formation of new muscle tissue in the damaged areas and to the reduction of forming fibrosis septa, which stimulates local neoangiogenesis that helps to form a complete vascular wall.Рубец на матке является одной из наиболее актуальных проблем современного акушерства, оказывая негативное влияние на репродуктивную функцию на всех этапах её реализации. Одним из перспективных направлений фундаментальных наук биомедицинского профиля является использование стволовых клеток для лечения различных состояний. В статье приведены данные экспериментального исследования, описывающего тканевые эффекты введения культуры мезенхимальных стволовых клеток в области рубца на матке после кесарева сечения. Доказан положительный эффект воздействия мезенхимальных стволовых клеток, способствующий образованию новой мышечной ткани в поврежденных участках и уменьшению формирующихся фиброзных септ, стимулирующий локальный неоангиогенез, помогающий формированию полноценной сосудистой стенки

CHARACTERISTICS OF BONE MARROW MICROENVIRONMENT IN MULTIPLE MYELOMA BEFORE AND AFTER TREATMENT

The purpose of the study is to evaluate the morphological and functional features of the tumor microenvironment and the cultural characteristics of mesenchymal stromal cells (MSC) of patients with multiple myeloma (MM) before and after treatment. Material and methods. Parenchymal and stromal bone marrow tissue in iliac bone trepanobiopsy of 32 patients with MM aged from 48 to 78 years (median age 56 years) before and after treatment was investigated. The study applied histological, immunohistochemical and cultural methods. Results and discussion. Сhanges of stromal microenvironment of bone marrow in all patients with MM have been revealed: increased microvascular density and the number of endosteal stromal cells, strengthening of reticulin fiber in subendosteal and perivascular spaces. Increased angiogenesis correlates with the number of plasma cells in the myelogram (r = 0.58; p < 0.05) and with the type of bone marrow infiltration (r = 0.85; р < 0.05), as well as with osteodestructive changes in the patient’s history (r = 0.65; p < 0.05). Significant changes in MSC cultures, both before and after therapy, including autologous hematopoietic stem-cell transplantation, were shown. Bone marrow MSC of patients with MM had decreased speed of proliferation (2.4 times on average), total time of passaging from 7 to 5 passages, increased expression of markers associated with myofibroblastic phenotype and aging (smooth muscle actin, β-galactosidase) compared to healthy donors. Conclusion. MSC cultures and BM niche forming elements of patients with MM have signs of tumor-associated microenvironment, despite the treatment of the underlying disease