314 research outputs found
Polymer sequencing by molecular machines: A framework for predicting the resolving power of a sliding contact force spectroscopy sequencing method
We evaluate an AFM-based single molecule force spectroscopy method for mapping sequences in otherwise difficult to sequence heteropolymers, including glycosylated proteins and glycans. The sliding contact force spectroscopy (SCFS) method exploits a sliding contact made between a nanopore threaded over a polymer axle and an AFM probe. We find that for sliding α- and β- cyclodextrin nanopores over a wide range of hydrophilic monomers, the free energy of sliding is proportional to the sum of two dimensionless, easily calculable parameters representing the relative partitioning of the monomer inside the nanopore or in the aqueous phase, and the friction arising from sliding the nanopore over the monomer. Using this relationship we calculate sliding energies for nucleic acids, amino acids, glycan and synthetic monomers and predict on the basis of these calculations that SCFS will detect N- and O-glycosylation of proteins and patterns of sidechains in glycans. For these applications, SCFS offers an alternative to sequence mapping by mass spectrometry or newly-emerging nanopore technologies that may be easily implemented using a standard AFM
Luminescent complexes of the zinc triad with N-substituted 8-amino-quinoline ligands: Synthesis and comparative study on the stability constants and related photophysical properties
Isothermal titration microcalorimetry to determine the thermodynamics of metal ion removal by magnetic nanoparticle sorbents
Applying ITC to determine key thermodynamic parameters needed to model the sorption of metal ions by magnetic nanoparticles sorbents
Enthalpy of solvation correlations for gaseous solutes dissolved in dimethyl sulfoxide and propylene carbonate based on the Abraham model
Equations for the Transfer of Neutral Molecules and Ionic Species from Water to Organic phases
ChemInform Abstract: THE EFFECT OF INTERIONIC INTERACTIONS ON SELECTIVITY IN UNI-BIVALENT CATION EXCHANGE SYSTEMS
Diethyl sulfide modified silica and calix[4]pyrrole chelating resin: Synthesis and mercury(ii) cation retention properties
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