212 research outputs found
«Ein (Sprach-)Bild sagt mehr als tausend Worte»: Arbeiten mit Metaphern in der Laufbahnberatung : eine qualitative Untersuchung
Metaphern als bildsprachliches PhĂ€nomen sind in unserer Alltagssprache ubiquitĂ€r und aus kommunikativen Prozessen wie BeratungsgesprĂ€chen nicht wegzudenken. Die vorliegende Arbeit befasst sich mit dem Einsatz von Metaphern als Arbeitsinstrument in der Laufbahnberatung. WĂ€hrend die Verwendung von Sprachbildern im Kontext der therapeutisch-beraterischen Konversation allgemein gut erforscht ist, gibt es nur wenige wissenschaftliche Veröffentlichungen aus dem spezifischen Bereich der Berufs-, Studien- und Laufbahnberatung. Ein Hauptfokus dieser Arbeit ist daher die Gewinnung von Erkenntnissen bezĂŒglich eingesetzter Techniken und Methoden der Beratungsarbeit mit Sprachbildern in der Praxis der Laufbahnberatung. Ausserdem stehen die Möglichkeiten und Grenzen der Verwendung dieses Instruments im Zentrum des Interesses. Ziel dieser Arbeit ist es, wissenschaftliche Erkenntnisse zum Thema und die Ergebnisse der durchgefĂŒhrten empirischen Untersuchung zusammenzufĂŒhren und daraus Empfehlungen fĂŒr die praktische Arbeit mit Metaphern im Rahmen der Laufbahnberatung zu formulieren
Photophysics, Molecular Reorientation in Solution and X-Ray Structure of a New Fluorescent Probe 1,7-Diazaperylene
A new fluorescent molecule 1,7-diazaperylene (DP) has been investigated by means of time-resolved and steady-state polarized fluorescence spectroscopy, as well as X-ray spectroscopy. Absorption and fluorescence spectra of DP in solution are similar to those of perylene. However, absorption and fluorescence spectra of 2,8-dimethoxy DP and 2,8-dipentyloxy DP in solution are red-shifted by ca. 55 nm relative to perylene. The fluorescence decay of DP is exponential with a lifetime of 5.1 ns in ethanol, 4.9 ns in glycerol and 4.3 ns in paraffin oil. The radiative lifetime in ethanol was calculated to be 6.3 ns for DP, 8.0 ns for 2,8-dimethoxy DP and 7.6 ns for 2,8-dipentyloxy DP. The calculated fluorescence quantum yields of 0.8 for DP and its alkoxy derivatives in ethanol, are in good agreement with those obtained from measurements. The calculated Förster radius is 37.2 ± 1 Ă
for DP and 41.9 ± 1 Ă
for its alkoxy derivatives in ethanol. Examining the S0 S1 transition, we obtain a limiting fluorescence anisotropy of r0 0.38 for DP and its alkoxy derivatives. The rotational rates of DP in paraffin oil and glycerol were compared to that of perylene. In paraffin oil both molecules show an almost identical biexponential decay of the fluorescence anisotropy, which is compatible with a rotational motion like an oblate ellipsoid. The fluorescence anisotropy is monoexponential for DP in glycerol, and DP appears to rotate like a spherical particle while perylene in glycerol appears to rotate like an oblate ellipsoid. Moreover, the rotational diffusion constant, corresponding to rotation about an axis in the aromatic plane (D), is the same for both DP and perylene in glycerol
Alteromonas Myovirus V22 Represents a New Genus of Marine Bacteriophages Requiring a Tail Fiber Chaperone for Host Recognition
Marine phages play a variety of critical roles in regulating the microbial composition of our oceans. Despite constituting the majority of genetic diversity within these environments, there are relatively few isolates with complete genome sequences or in-depth analyses of their host interaction mechanisms, such as characterization of their receptor binding proteins (RBPs). Here, we present the 92,760-bp genome of the Alteromonas-targeting phage V22. Genomic and morphological analyses identify V22 as a myovirus; however, due to a lack of sequence similarity to any other known myoviruses, we propose that V22 be classified as the type phage of a new Myoalterovirus genus within the Myoviridae family. V22 shows gene homology and synteny with two different subfamilies of phages infecting enterobacteria, specifically within the structural region of its genome. To improve our understanding of the V22 adsorption process, we identified putative RBPs (gp23, gp24, and gp26) and tested their ability to decorate the V22 propagation strain, Alteromonas mediterranea PT11, as recombinant green fluorescent protein (GFP)-tagged constructs. Only GFP-gp26 was capable of bacterial recognition and identified as the V22 RBP. Interestingly, production of functional GFP-gp26 required coexpression with the downstream protein gp27. GFP-gp26 could be expressed alone but was incapable of host recognition. By combining size-exclusion chromatography with fluorescence microscopy, we reveal how gp27 is not a component of the final RBP complex but instead is identified as a new type of phage-encoded intermolecular chaperone that is essential for maturation of the gp26 RBP.This work was supported by grants âVIREVOâ CGL2016â76273âP (MCI/AEI/FEDER, EU) (cofounded with FEDER funds) from the Spanish Ministerio de Ciencia e InnovaciĂłn and âHIDRAS3â PROMETEU/2019/009 from Generalitat Valenciana. R.G.-S. was supported by a predoctoral fellowship from the Valencian ConsellerĂa de EducaciĂł, InvestigaciĂł, Cultura i Esport (ACIF/2016/050) and was also a beneficiary of the BEFPI 2019 fellowship for predoctoral stays from Generalitat Valenciana and The European Social Fund. F.R.-V. was a beneficiary of the 5top100 program of the Ministry for Science and Education of Russia
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DEMOLISHING A COLD WARE ERA FULE STORAGE BASIN SUPERSTRUCTURE LADEN WITH ASBESTOS
The K East (KE) Basin facilities are located near the north end of the Hanford Site's 100 K area. The facilities were built in 1950 as part of the KE Reactor complex and constructed within 400 meters of the Columbia River, which is the largest river in the Pacific Northwest and by volume the fourth largest river in the United States. The basin, located adjacent to the reactor, was used for the underwater storage of irradiated nuclear fuel discharged from the reactor. The basin was covered by a superstructure comprising steel columns and beams, concrete, and cement asbestos board (CAB) siding. The project's mission was to complete demolition of the structure over the KE Basin within six months of turnover from facility deactivation activities. The demolition project team applied open-air demolition techniques to bring the facility to slab-on-grade. Several innovative techniques were used to control contamination and maintain contamination control within the confines of the demolition exclusion zone. The techniques, which focused on a defense-in-depth approach, included spraying fixatives on interior and exterior surfaces before demolition began; applying fixatives during the demolition; misting using a fine spray of water during demolition; and demolishing the facility systematically. Another innovative approach that made demolition easier was to demolish the building with the non-friable CAB remaining in place. The CAB siding covered the exterior of the building and portions of the interior walls, and was an integral part of the multiple-layered roof. The project evaluated the risks involved in removing the CAB material in a radiologically contaminated environment and determined that radiological dose rates and exposure to radiological contamination and industrial hazards would be significantly reduced by using heavy equipment to remove the CAB during demolition. The ability to perform this demolition safely and without spreading contamination (radiological or asbestos) demonstrates that contaminated structures can be torn down successfully using similar open-air demolition techniques
Systemic application of bone-targeting peptidoglycan hydrolases as a novel treatment approach for staphylococcal bone infection
The rising prevalence of antimicrobial resistance in S. aureus has rendered treatment of staphylococcal infections increasingly difficult, making the discovery of alternative treatment options a high priority. Peptidoglycan hydrolases, a diverse group of bacteriolytic enzymes, show high promise as such alternatives due to their rapid and specific lysis of bacterial cells, independent of antibiotic resistance profiles. However, using these enzymes for the systemic treatment of local infections, such as osteomyelitis foci, needs improvement, as the therapeutic distributes throughout the whole host, resulting in low concentrations at the actual infection site. In addition, the occurrence of intracellularly persisting bacteria can lead to relapsing infections. Here, we describe an approach using tissue-targeting to increase the local concentration of therapeutic enzymes in the infected bone. The enzymes were modified with a short targeting moiety that mediated accumulation of the therapeutic in osteoblasts and additionally enables targeting of intracellularly surviving bacteria
Clinical and radiographic evaluation of early loaded narrow diameter implants â 1âyear followâup
Objective To evaluate the clinical performance of A stra T ech O sseo S peedâą TX 3.0 S implants using oneâstage surgical procedure and early loading in the anterior region. Material and methods This is a prospective, single arm, multiâcentre study. Patients missing teeth at positions 12, 22 and 32â42 were eligible to enter the study. The implants ( O sseo S peedâą TX 3.0 S ) used in the study were of 3Â mm diameter and of different lengths. Oneâstage surgery was performed, and healing abutments were used during the 6â10Â weeks healing period. Clinical and radiographic examinations were assessed at implant installation, loading and at the 6â and 12âmonth followâup visits. Results Ninetyâseven implants were placed in 69 patients at six different study centres in D enmark, G ermany, I taly, S pain, S weden and the U nited K ingdom. The survival rate was 95.9%. No implants have been lost after loading (100% survival rate after loading). Mean marginal bone loss 1Â year after installation was 0.065Â mm ( SD Â =Â 1.018). The frequency of bone loss â„1Â mm was 6.6% and 51.3% of the implants demonstrated no bone loss or even bone gain from the surgical visit to the first year followâup visit. Mean probing pocket depth and gingival zenith score were stable from crown placement to the 6â and 1âyear followâup visits. Conclusion Treatment with O sseo S peedâą TX 3.0 S implants is a safe and predictable option in the anterior region where physical space is limited. Minimal marginal bone loss was observed during the first year followâup.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/91103/1/clr2254.pd
A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids
Indexación: Scopus.The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids (f-ncAA) have been successfully incorporated in proteins expressed in bacterial systems, Xenopus oocytes, and HEK-293T cells. Here, we describe the rational generation of a novel orthogonal aminoacyl-tRNA synthetase based on the E. coli tyrosine synthetase that is capable of encoding the f-ncAA tyr-coumarin in HEK-293T cells. © 2020Bioorganic Chemistry; Bioinformatics; Proteins; Biochemistry; Molecular Biology; Unnatural amino acids, aminoacyl-tRNA synthetase, coumarin, fluorescence. © 2020https://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S2405844020319836?via%3Dihu
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