Investigation of the Dietary Intake and Health Status in East Africa in the 1960s: A Systematic Review of the Historic Oltersdorf Collection

We have recently reported on the myriad health benefits of traditional East African foods and food habits. However, this region continues to experience a nutrition transition whereby traditional, well-tried foods have been systematically replaced with the products of multinational corporations. The health-related impact has been devastating, as evidenced by current non-communicable disease (NCD) trends. The purpose of the present investigation was to review the historic Oltersdorf Collection (data collated by the Max-Planck Nutrition Research Unit, Bumbuli Tanzania from the 1930s to 1960s) to determine if adherence to traditional East African food habits was positively associated with health status indices in populations residing in Tanzania, Kenya and Uganda during this period. The systematic review process resulted in six investigations being identified. Published between 1963 and 1969, these are likely the first investigations to provide original data pertaining to dietary intake/adequacy and health status indices within specific East African cohorts. Overall, the review revealed that many ethnic groups did not exhibit adequate dietary intake and did not consume a diversity of traditional whole foods representative of the wide spectrum of food choices available within the region at this time. NCDs such as obesity, hypertension, and type 2 diabetes were not reported in any investigation. However, there was substantial reporting of malnutrition-related and infectious diseases, particularly among children. The present review supports the contention that the shift from a traditional, diversified diet to a simplified, monotonous diet may have occurred with the onset of cash-crop farming. For resolution of nutrition-related epidemics currently plaguing Africa, including NCDs and malnutrition-related diseases (i.e., the double burden) it is critically important to investigate and disseminate evidence related to the fundamental contributors to the nutrition transition

Mild and Highly Flexible Enzyme-Catalyzed Modification of Poly (ethersulfone) Membranes

Poly(ethersulfone) (PES) membranes are widely used in industry for separation and purification purposes. However, the drawback of this type of membranes is fouling by proteins. For that reason, modification of PES membranes has been studied to enhance their protein repellence. This paper presents the first example of enzyme-catalyzed modification of PES membranes. Various phenolic acids (enzyme substrates) were bound to a membrane under very mild conditions (room temperature, water, nearly neutral pH) using only laccase from Trametes versicolor as catalyst. The extent of modification, monitored, for example, by the coloration of the modified membranes, can be tuned by adjusting the reaction conditions. The most significant results were obtained with 4-hydroxybenzoic acid and gallic acid as substrates. The presence of a covalently bound layer of 4-hydroxybenzoic acid on the grafted membranes was confirmed by X-ray photoelectron spectroscopy (XPS), infrared reflection absorption spectroscopy (IRRAS), and NMR. In the case of gallic acid, PES membrane modification is mainly caused by adsorption of enzymatically formed homopolymer. The ionization potential of the substrates, and the electronic energies and spin densities of the radicals that are intermediates in the attachment reaction were calculated (B3LYP/6-311G(d,p)) to determine the reactive sites and the order of reactivity of radical substrates to couple with the PES membrane. The calculated order of reactivity of the substrates is in line with the experimental observations. The calculated spin densities in the phenolic radicals are highest at the oxygen atom, which is in line with the formation of ether linkages as observed by IRRAS. The liquid fluxes of the modified membranes are hardly influenced by the grafted layers, in spite of the presence of a substantial and stable new layer, which opens a range of application possibilities for these modified membrane