Synthesis and analysis of Cu(II) and Co(II) complexes of Schiff base derived from glycine and isatin

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Injection safety practices in a main referral hospital in northeastern Nigeria

Background: No adherence of safe injection policies remains a major challenge, and, worldwide, annually, it leads to 21 million new hepatitis B cases and 260,000 HIV infection cases. This descriptive observational survey was conducted to determine the level of adherence to universal precaution for safe injection practices in the hospital.Materials and Methods: The study units were selected using a simple random sampling of injection services provider/ phlebotomist in 27 units/wards of the hospital. The study instruments were observation checklist and interviewer administered questionnaires. EPI info (version 3.5.2) software was used for data entry and generation of descriptive statistics was done with units of analysis (units/wards) on injection safety practices of health workers, availability of logistics and supplies, and disposal methods.Results: Only 33.3% of the units (95% CI, 16‑54) had non‑sharps infectious healthcare waste of any type inside containers specific for non‑sharps infectious waste and 17 (77.3%) of the observed therapeutic  injections were prepared on a clean, dedicated table or tray, where contamination of the equipment with blood, body fluids, or dirty swabs was unlikely. Absence of recapping of needles was observed in 11 (50.0%) units giving therapeutic injections. Only 7.4% of units surveyed had separate waste containers for infectious non‑sharps.Conclusions: This study depicts poor knowledge and a practice of injection safety, inadequate injection safety supplies, and non‑compliance to injection safety policy and guidelines.Key words: Injection waste management, safe injections practices, tertiary health care facilit

Health Risks Associated with Consumption of Vegetables Grown using Domestic Wastewater in Minna, Niger State, Nigeria

Most vegetables consumed in the cities of developing countries are grown using wastewater discharged through township drainage network. This paper, therefore, investigates the risk involved in the consumption of such vegetables. The human daily intake rate (DIR) and Health Risk Index (HRI) of heavy metals were calculated using established formulae and the values of DIR ranged from 0.10 mg/kg/day to 0.71 mg/kg/day in wastewater plots in dry season for adults and 0.10 mg/kg/day to 0.14 mg/kg/day for children. Health risk index (HRI) values ranges from 0.40 mg/kg/day to 0.75 mg/kg/day in wastewater plots for adults in dry season and 0.20 mg/kg/day to 0.95 mg/kg/day for children. In wet season wastewater plots, the HRI ranges from 0.40 mg/kg/day to 0.95 mg/kg/day for children. The values of Hazard index (HI) ranged between 1.00 mg/kg/day and 1.21 mg/kg/day for all the seasons. Statistical analysis showed that there is a significant difference between the wet and dry season values for all the parameters assessed. Finally, uptake of heavy metals from the soil by all the vegetable crops under investigation was established. Therefore proper monitoring needs to be carried out to regulate consumption of vegetables produced from the experimental sites as continuous application of the industrial wastewater may lead to further accumulation of these heavy metals

Assessment of Heavy Metals Uptake by Vegetables Cultivated on Soil Receiving Industrial Wastewater in Minna, Nigeria

Consumption of vegetable crops grown on soil irrigated with industrial wastewater has been the order of the day in most urban towns and cities of Nigeria, despite reports of its serious health impact. This study assessed the possibility of uptake of heavy metals by crops grown on soil receiving industrial wastewater during rainy and dry seasons. The crops investigated are Spinach, Cayenne Pepper, Jute mallow, roselle and lady’s fingers okra. The initial composition of the wastewater was analyzed to contain 0.89 mg/l of chromium, 0.74 mg/l of cadmium, 1.04 mg/l of copper and 2.81 mg/l of iron. Control water used for this experiment contain no trace of any of these heavy metals. The experimental soil was also analyzed and contained 0.10 mg/kg of chromium, 0.06 mg/kg of iron before irrigation in dry season. After irrigation at the wastewater plots, the heavy metal concentrations in soil had increased to 6.24 mg/kg of chromium and 7.50 mg/kg of iron. In wet season, the concentrations of heavy metals in the soil were 0.00 mg/kg of chromium and 2.32 mg/kg of iron before irrigation. After irrigation in wet season, the concentrations increased to 6.01 mg/kg of iron. Mean difference of heavy metal concentrations were significantly high in vegetables in dry season, with values ranging from 0.03 mg/kg to 211mg/kg in wastewater plots, 0.20 mg/kg to 215 mg/kg in wet seasons wastewater plots, 0.00 mg/kg to 157 mg/kg in dry season. It is however recommended that consumption of vegetables irrigated with domestic/industrial wastewater be strongly discouraged because of its serious health implications.&nbsp

Rapid Ethical Appraisal: A tool to design a contextualized consent process for a genetic study of podoconiosis in Ethiopia

Background: Obtaining genuine informed consent from research participants in developing countries can be difficult, partly due to poor knowledge about research process and research ethics. The situation is complicated when conducting genomic research on a disease considered familial and a reason for stigmatisation. Methods: We used a Rapid Ethical Appraisal tool to assess local factors that were barriers to getting genuine informed consent prior to conducting a genetic study of podoconiosis (non-filarial elephantiasis) in two Zones of Ethiopia. The tool included in-depth interviews and focus group discussions with patients, healthy community members, field workers, researchers/Institutional Review Board (IRB) members, elders, religious leaders, and podoconiosis administrators who work closely with patients. Results: Most patients and healthy community members did not differentiate research from routine clinical diagnosis. Participants felt comfortable when approached in the presence of trusted community members. Field workers and podoconiosis administrators preferred verbal consent, whereas the majority of patients and healthy community members prefer both verbal and written consent. Participants better understood genetic susceptibility concepts when analogies drawn from their day-to-day experience were used. The type of biological sample sought and gender were the two most important factors affecting the recruitment process. Most researchers and IRB members indicated that reporting incidental findings to participants is not a priority in an Ethiopian context. Conclusions: Understanding the concerns of local people in areas where research is to be conducted facilitates the design of contextualized consent processes appropriate for all parties and will ultimately result in getting genuine consent

Redox Modulation at Work: Natural Phytoprotective Polysulfanes From Alliums Based on Redox-Active Sulfur

Purpose of review: This article provides a brief overview of natural phytoprotective products of allium with a special focus on the therapeutic potential of diallyl polysulfanes from garlic, their molecular targets and their fate in the living organisms. A comprehensive overview of antimicrobial and anticancer properties of published literature is presented for the reader to understand the effective concentrations of polysulfanes and their sensitivity towards different human pathogenic microbes, fungi, and cancer cell lines. Recent findings: The article finds polysulfanes potentials as new generation novel antibiotics and chemo preventive agent. The effective dose rates of polysulfanes for antimicrobial properties are in the range of 0.5–40 mg/L and for anticancer 20–100 μM. The molecular targets for these redox modulators are mainly cellular thiols as well as inhibition and/or activation of certain cellular proteins in cancer cell lines. Summary: Antimicrobial and anticancer activities of polysulfanes published in the literature indicate that with further development, they could be promising candidates for cancer prevention due to their selectivity towards abnormal cells

Permeation Mechanisms in the TMEM16B Calcium-Activated Chloride Channels

TMEM16A and TMEM16B encode for Ca2+-activated Cl- channels (CaCC) and are expressed in many cell types and play a relevant role in many physiological processes. Here, I performed a site-directed mutagenesis study to understand the molecular mechanisms of ion permeation of TMEM16B. I mutated two positive charged residues R573 and K540, respectively located at the entrance and inside the putative channel pore and I measured the properties of wild-type and mutant TMEM16B channels expressed in HEK-293 cells using whole-cell and excised inside-out patch clamp experiments. I found evidence that R573 and K540 control the ion permeability of TMEM16B depending both on which side of the membrane the ion substitution occurs and on the level of channel activation. Moreover, these residues contribute to control blockage or activation by permeant anions. Finally, R573 mutation abolishes the anomalous mole fraction effect observed in the presence of a permeable anion and it alters the apparent Ca2+-sensitivity of the channel. These findings indicate that residues facing the putative channel pore are responsible both for controlling the ion selectivity and the gating of the channel, providing an initial understanding of molecular mechanism of ion permeation in TMEM16B

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

© 2024 The Authors. Journal of Extracellular Vesicles, published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.Peer reviewe