Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification.

The objective of the present study was to evaluate the effects of supplementation of in vitro culture (IVC) medium with drugs that stimulates the lipolysis (Forskolin: Forsk) and inhibit the lipogenesis (Linoleic Acid LA) on the intracytoplasmic lipid content and cryotolerance of bovine embryos (Experiment 1), as well as to evaluate the effect of treatment of embryos with Forsk on the pregnancy rates after transfer to synchronized recipients (Experiment 2).Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A327 Support Biotechnologies: Cryopreservation and cryobiology, diagnosis through imaging, molecular biology and ?omics?. Título em português: Criotolerância e taxa de concepção após exposição de embriões bovinos produzidos in vitro ao Forskolin ou ácido linoleico antes da vitrificação

Effect of dietary supplementation of Nelore heifers with polyunsaturated fatty acids on cryotolerance and membrane lipid profile of fresh and vitrified in vitro-produced embryos.

The aim of this study was to evaluate the development and cryotolerance of IVP embryos from oocytes recovered from Nelore heifers (n = 16) fed with control diet (Control group) or fed with rumen-protected PUFA (Megalac-E®), for at least 60 days (Fat group).Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A326. Support Biotechnologies: Cryopreservation and cryobiology, diagnosis through imaging, molecular biology and “omics”. Título em português: Influência da suplementação alimentar de novilhas Nelore com ácidos graxos poliinsaturados sobre a criotolerância e o perfil de lipídios de membrana de embriões produzidos in vitro frescos e vitrificados

Magnetic Flux Transport by turbulent reconnection in astrophysical flows

The role of MHD turbulence in astrophysical environments is still highly debated. An important question that permeates this debate is the transport of magnetic flux. This is particularly important, for instance, in the context of star formation. When clouds collapse gravitationally to form stars, there must be some magnetic flux transport. otherwise the new born stars would have magnetic fields several orders of magnitude larger than the observed ones. Also, the magnetic flux that is dragged in the late stages of the formation of a star can remove all the rotational support from the accretion disk that grows around the protostar. The efficiency of the mechanism which is often invoked to allow the transport of magnetic fields in the different stages of star formation, namely, the ambipolar diffusion, has been lately put in check. We here discuss an alternative mechanism for magnetic flux transport which is based on turbulent fast magnetic reconnection. We review recent results obtained from 3D MHD numerical simulations that indicate that this mechanism is very efficient for decoupling and transport magnetic flux from the inner denser regions to the outskirts of collapsing clouds in the different stages of star formation. We also discuss this mechanism in the context of dynamo processes and speculate that it can play a role both in the solar dynamo and in accretion disk dynamo processes.Comment: 10 pages, 2 figures, review submitted to Physica Script

Anesthesiology Consensus in the Management of the Airway

Os consensos na gestão clínica da via aérea em anestesiologia pretendem disponibilizar informação, baseada na evidência atual ou, na falta desta, na opinião de peritos, no que respeita à abordagem da via aérea difícil previsível ou não previsível. Reforçamos a importância da avaliação da via aérea e da identificação de potenciais problemas que possam condicionar dificuldade na sua abordagem e a adoção de uma estratégia segura que permita identificar e responder em crescendo de intervenção às dificuldades encontradas. Na impossibilidade de intubação traqueal (não intubo) otimizada e limitada a 4 tentativas, da impossibilidade de ventilar e oxigenar (não oxigeno) após 2 tentativas de usar um dispositivo supraglótico ou de uso de máscara facial inicialmente adequada é importante realizar, em tempo útil, uma cricotirotomia para assegurar oxigenação. As situações clínicas de exceção só com planos simples, conhecidos por todos e regularmente treinados e adaptados à nossa atividade clinica podem assegurar melhores “outcomes”. O registo destes eventos e a informação ao nosso doente da dificuldade encontrada e modo como foi resolvido o problema é essencial e constitui ainda um desafio a alargar a uma base nacional.info:eu-repo/semantics/publishedVersio

Reconstruction of inclined air showers detected with the Pierre Auger Observatory

We describe the method devised to reconstruct inclined cosmic-ray air showers with zenith angles greater than $60^\circ$ detected with the surface array of the Pierre Auger Observatory. The measured signals at the ground level are fitted to muon density distributions predicted with atmospheric cascade models to obtain the relative shower size as an overall normalization parameter. The method is evaluated using simulated showers to test its performance. The energy of the cosmic rays is calibrated using a sub-sample of events reconstructed with both the fluorescence and surface array techniques. The reconstruction method described here provides the basis of complementary analyses including an independent measurement of the energy spectrum of ultra-high energy cosmic rays using very inclined events collected by the Pierre Auger Observatory.Comment: 27 pages, 19 figures, accepted for publication in Journal of Cosmology and Astroparticle Physics (JCAP

A search for point sources of EeV photons

Measurements of air showers made using the hybrid technique developed with the fluorescence and surface detectors of the Pierre Auger Observatory allow a sensitive search for point sources of EeV photons anywhere in the exposed sky. A multivariate analysis reduces the background of hadronic cosmic rays. The search is sensitive to a declination band from -85{\deg} to +20{\deg}, in an energy range from 10^17.3 eV to 10^18.5 eV. No photon point source has been detected. An upper limit on the photon flux has been derived for every direction. The mean value of the energy flux limit that results from this, assuming a photon spectral index of -2, is 0.06 eV cm^-2 s^-1, and no celestial direction exceeds 0.25 eV cm^-2 s^-1. These upper limits constrain scenarios in which EeV cosmic ray protons are emitted by non-transient sources in the Galaxy.Comment: 28 pages, 10 figures, accepted for publication in The Astrophysical Journa

The Pierre Auger Observatory III: Other Astrophysical Observations

Astrophysical observations of ultra-high-energy cosmic rays with the Pierre Auger ObservatoryComment: Contributions to the 32nd International Cosmic Ray Conference, Beijing, China, August 201

Measurement of the Depth of Maximum of Extensive Air Showers above 10^18 eV

We describe the measurement of the depth of maximum, Xmax, of the longitudinal development of air showers induced by cosmic rays. Almost four thousand events above 10^18 eV observed by the fluorescence detector of the Pierre Auger Observatory in coincidence with at least one surface detector station are selected for the analysis. The average shower maximum was found to evolve with energy at a rate of (106 +35/-21) g/cm^2/decade below 10^(18.24 +/- 0.05) eV and (24 +/- 3) g/cm^2/decade above this energy. The measured shower-to-shower fluctuations decrease from about 55 to 26 g/cm^2. The interpretation of these results in terms of the cosmic ray mass composition is briefly discussed.Comment: Accepted for publication by PR