417 research outputs found
Functionalized Mesoporous SBA-15 with CeF3: Eu3+ Nanoparticle by Three Different Methods: Synthesis, Characterization, and Photoluminescence
Luminescence functionalization of the ordered mesoporous SBA-15 silica is realized by depositing a CeF3: Eu3+ phosphor layer on its surface (denoted as CeF3: Eu3+/SBA-15/IS, CeF3: Eu3+/SBA-15/SI and CeF3: Eu3+/SBA-15/SS) using three different methods, which are reaction in situ (I-S), solution impregnation (S-I) and solid phase grinding synthesis (S-S), respectively. The structure, morphology, porosity, and optical properties of the materials are well characterized by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, N2 adsorption, and photoluminescence spectra. These materials all have high surface area, uniformity in the mesostructure and crystallinity. As expected, the pore volume, surface area, and pore size of SBA-15 decrease in sequence after deposition of the CeF3: Eu3+ nanophosphors. Furthermore, the efficient energy transfer in mesoporous material mainly occurs between the Ce3+ and the central Eu3+ ion. They show the characteristic emission of Ce3+ 5d → 4f (200–320 nm) and Eu3+5D0 → 7FJ(J = 1–4, with 5D0 → 7F1 orange emission at 588 nm as the strongest one) transitions, respectively. In addition, for comparison, the mesoporous material CeF3: Eu3+/SBA-15/SS exhibits the characteristic emission of Eu3+ ion under UV irradiation with higher luminescence intensity than the other materials
miR-200 Enhances Mouse Breast Cancer Cell Colonization to Form Distant Metastases
BACKGROUND: The development of metastases involves the dissociation of cells from the primary tumor to penetrate the basement membrane, invade and then exit the vasculature to seed, and colonize distant tissues. The last step, establishment of macroscopic tumors at distant sites, is the least well understood. Four isogenic mouse breast cancer cell lines (67NR, 168FARN, 4TO7, and 4T1) that differ in their ability to metastasize when implanted into the mammary fat pad are used to model the steps of metastasis. Only 4T1 forms macroscopic lung and liver metastases. Because some miRNAs are dysregulated in cancer and affect cellular transformation, tumor formation, and metastasis, we examined whether changes in miRNA expression might explain the differences in metastasis of these cells. METHODOLOGY/PRINCIPAL FINDINGS: miRNA expression was analyzed by miRNA microarray and quantitative RT-PCR in isogenic mouse breast cancer cells with distinct metastatic capabilities. 4T1 cells that form macroscopic metastases had elevated expression of miR-200 family miRNAs compared to related cells that invade distant tissues, but are unable to colonize. Moreover, over-expressing miR-200 in 4TO7 cells enabled them to metastasize to lung and liver. These findings are surprising since the miR-200 family was previously shown to promote epithelial characteristics by inhibiting the transcriptional repressor Zeb2 and thereby enhancing E-cadherin expression. We confirmed these findings in these cells. The most metastatic 4T1 cells acquired epithelial properties (high expression of E-cadherin and cytokeratin-18) compared to the less metastatic cells. CONCLUSIONS/SIGNIFICANCE: Expression of miR-200, which promotes a mesenchymal to epithelial cell transition (MET) by inhibiting Zeb2 expression, unexpectedly enhances macroscopic metastases in mouse breast cancer cell lines. These results suggest that for some tumors, tumor colonization at metastatic sites might be enhanced by MET. Therefore the epithelial nature of a tumor does not predict metastatic outcome
J/psi suppression at forward rapidity in Au+Au collisions at sqrt(s_NN)=39 and 62.4 GeV
We present measurements of the J/psi invariant yields in sqrt(s_NN)=39 and
62.4 GeV Au+Au collisions at forward rapidity (1.2<|y|<2.2). Invariant yields
are presented as a function of both collision centrality and transverse
momentum. Nuclear modifications are obtained for central relative to peripheral
Au+Au collisions (R_CP) and for various centrality selections in Au+Au relative
to scaled p+p cross sections obtained from other measurements (R_AA). The
observed suppression patterns at 39 and 62.4 GeV are quite similar to those
previously measured at 200 GeV. This similar suppression presents a challenge
to theoretical models that contain various competing mechanisms with different
energy dependencies, some of which cause suppression and others enhancement.Comment: 365 authors, 10 pages, 11 figures, 4 tables. Submitted to Phys. Rev.
C. Plain text data tables for the points plotted in figures for this and
previous PHENIX publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Nuclear matter effects on production in asymmetric Cu+Au collisions at = 200 GeV
We report on production from asymmetric Cu+Au heavy-ion collisions
at =200 GeV at the Relativistic Heavy Ion Collider at both
forward (Cu-going direction) and backward (Au-going direction) rapidities. The
nuclear modification of yields in CuAu collisions in the Au-going
direction is found to be comparable to that in AuAu collisions when plotted
as a function of the number of participating nucleons. In the Cu-going
direction, production shows a stronger suppression. This difference is
comparable in magnitude and has the same sign as the difference expected from
shadowing effects due to stronger low- gluon suppression in the larger Au
nucleus. The relative suppression is opposite to that expected from hot nuclear
matter dissociation, since a higher energy density is expected in the Au-going
direction.Comment: 349 authors, 10 pages, 4 figures, and 4 tables. Submitted to Phys.
Rev. C. For v2, fixed LaTeX error in 3rd-to-last sentence. Plain text data
tables for the points plotted in figures for this and previous PHENIX
publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Medium modification of jet fragmentation in Au+Au collisions at sqrt(s_NN)=200 GeV measured in direct photon-hadron correlations
The jet fragmentation function is measured with direct photon-hadron
correlations in p+p and Au+Au collisions at sqrt(s_NN)=200 GeV. The p_T of the
photon is an excellent approximation to the initial p_T of the jet and the
ratio z_T=p_T^h/p_T^\gamma is used as a proxy for the jet fragmentation
function. A statistical subtraction is used to extract the direct photon-hadron
yields in Au+Au collisions while a photon isolation cut is applied in p+p. I_
AA, the ratio of jet fragment yield in Au+Au to that in p+p, indicates
modification of the jet fragmentation function. Suppression, most likely due to
energy loss in the medium, is seen at high z_T. The fragment yield at low z_T
is enhanced at large angles. Such a trend is expected from redistribution of
the lost energy into increased production of low-momentum particles.Comment: 562 authors, 70 insitutions, 8 pages, and 3 figures. Submitted to
Phys. Rev. Lett. v2 has minor changes to improve clarity. Plain text data
tables for the points plotted in figures for this and previous PHENIX
publications are (or will be) publicly available at
http://www.phenix.bnl.gov/papers.htm
Measurement of (1S+2S+3S) production in and AuAu collisions at GeV
Measurements of bottomonium production in heavy ion and collisions
at the Relativistic Heavy Ion Collider (RHIC) are presented. The inclusive
yield of the three states, , was measured in the
PHENIX experiment via electron-positron decay pairs at midrapidity for AuAu
and collisions at GeV. The
differential cross section at
midrapidity was found to be 108 38 (stat)
15(syst) 11 (luminosity) pb in collisions. The nuclear
modification factor in the 30\% most central AuAu collisions indicates a
suppression of the total state yield relative to the extrapolation
from collision data. The suppression is consistent with measurements
made by STAR at RHIC and at higher energies by the CMS experiment at the Large
Hadron Collider.Comment: 506 authors, 15 pages, 17 figures, and 7 tables. v3 is as accepted by
Phys. Rev. C. v2 has changes to text and figures, plus additional authors.
Published version will be at
http://www.phenix.bnl.gov/phenix/WWW/info/pp1/1NN/ Plain text data tables are
(or will be) at http://www.phenix.bnl.gov/papers.htm
MiR-128 Inhibits Tumor Growth and Angiogenesis by Targeting p70S6K1
MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-128 expression levels were decreased in glioma, and identified p70S6K1 as a novel direct target of miR-128. Overexpression of miR-128 suppressed p70S6K1 and its downstream signaling molecules such as HIF-1 and VEGF expression, and attenuated cell proliferation, tumor growth and angiogenesis. Forced expression of p70S6K1 can partly rescue the inhibitory effect of miR-128 in the cells. Taken together, these findings will shed light to the role and mechanism of miR-128 in regulating glioma tumor angiogenesis via miR-128/p70S6K1 axis, and miR-128 may serve as a potential therapeutic target in glioma in the future
Nur77 regulates lipolysis in skeletal muscle cells - Evidence for cross-talk between the beta-adrenergic and an orphan nuclear hormone receptor pathway
Skeletal muscle is a major mass peripheral tissue that accounts for similar to 40% of total body weight and 50% of energy expenditure and is a primary site of glucose disposal and fatty acid oxidation. Consequently, muscle has a significant role in insulin sensitivity, obesity, and the blood-lipid profile. Excessive caloric intake is sensed by the brain and induces beta-adrenergic receptor (beta-AR)- mediated adaptive thermogenesis. beta-AR null mice develop severe obesity on a high fat diet. However, the target gene(s), target tissues(s), and molecular mechanism involved remain obscure. We observed that 30 - 60 min of beta-AR agonist ( isoprenaline) treatment of C2C12 skeletal muscle cells strikingly activated (> 100-fold) the expression of the mRNA encoding the nuclear hormone receptor, Nur77. In contrast, the expression of other nuclear receptors that regulate lipid and carbohydrate metabolism was not induced. Stable transfection of Nur77-specific small interfering RNAs (siNur77) into skeletal muscle cells repressed endogenous Nur77 mRNA expression. Moreover, we observed attenuation of gene and protein expression associated with the regulation of energy expenditure and lipid homeostasis, for example AMP-activated protein kinase gamma 3, UCP3, CD36,adiponectin receptor 2, GLUT4, and caveolin-3. Attenuation of Nur77 expression resulted in decreased lipolysis. Finally, in concordance with the cell culture model, injection and electrotransfer of siNur77 into mouse tibialis cranialis muscle resulted in the repression of UCP3 mRNA expression. This study demonstrates regulatory cross-talk between the nuclear hormone receptor and beta-AR signaling pathways. Moreover, it suggests Nur77 modulates the expression of genes that are key regulators of skeletal muscle lipid and energy homeostasis. In conclusion, we speculate that Nur77 agonists would stimulate lipolysis and increase energy expenditure in skeletal muscle and suggest selective activators of Nur77 may have therapeutic utility in the treatment of obesity
Вихретоковый анизотропный термоэлектрический первичный преобразователь лучистого потока
Представлена оригинальная конструкция первичного преобразователя лучистого потока, который может служить основой для создания приемника неселективного излучения с повышенной чувствительностью
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