Serotyping of Toxoplasma gondii Infection Using Peptide Membrane Arrays.

The intracellular parasite Toxoplasma gondii can cause chronic infections in most warm-blooded animals, including humans. In the USA, strains belonging to four different Toxoplasma clonal lineages (types 1, 2, 3, and 12) are commonly isolated, whereas strains not belonging to these lineages are predominant in other continents such as South America. Strain type plays a pivotal role in determining the severity of Toxoplasma infection. Therefore, it is epidemiologically relevant to develop a non-invasive and inexpensive method for determining the strain type in Toxoplasma infections and to correlate the genotype with disease outcome. Serological typing is based on the fact that many host antibodies are raised against immunodominant parasite proteins that are highly polymorphic between strains. However, current serological assays can only reliably distinguish type 2 from non-type 2 infections. To improve these assays, mouse, rabbit, and human infection serum were reacted against 950 peptides from 62 different polymorphic Toxoplasma proteins by using cellulose membrane peptide arrays. This allowed us to identify the most antigenic peptides and to pinpoint the most relevant polymorphisms that determine strain specificity. Our results confirm the utility of previously described peptides and identify novel peptides that improve and increase the specificity of the assay. In addition, a large number of novel proteins showed potential to be used for Toxoplasma diagnosis. Among these, peptides derived from several rhoptry, dense granule, and surface proteins represented promising candidates that may be used in future experiments to improve Toxoplasma serotyping. Moreover, a redesigned version of the published GRA7 typing peptide performed better and specifically distinguished type 3 from non-type 3 infections in sera from mice, rabbits, and humans

Exceptionally Bright Type Ib Supernova 1991D

Photometric and spectroscopic observations of the peculiar Type Ib Supernova 1991D are presented. SN 1991D was exceptionally bright for a Type Ib supernova. The He I lines were rather weak and the velocity at the photosphere as a function of time was unusually low. Comparison of the observed and synthetic spectra indicates that either hydrogen was ejected with a minimum velocity of 12,000 km/s or the spectrum contained features caused by lines of Ne I. Light curve modelling suggests that the progenitor probably had a very large radius (~10^14 cm) and that a considerable amount of 56Ni was synthesized during the explosion (~0.7 Msolar). We suggest a progenitor model of SN 1991D that involves a binary system.Comment: 7 pages, 9 figures, MNRAS in pres

Digital thErapy For Improved tiNnitus carE Study (DEFINE): protocol for a randomised controlled trial

Tinnitus is a common health condition, affecting approximately 15% of the UK population. The tinnitus treatment with the strongest evidence base is Cognitive Behavioural Therapy (CBT), with standard tinnitus therapy typically augmented with education, relaxation and other techniques. Availability of CBT and conventional tinnitus therapy more broadly is limited for tinnitus sufferers. The DEFINE trial aims to assess whether smartphone-delivered tinnitus therapy, the Oto app, is as effective as current standard care, one-to-one therapist-delivered tinnitus treatment for the treatment of tinnitus in adults. The trial is registered in the ISRCTN Registry: ISRCTN99577932. DEFINE is an open-label, non-inferiority, prospective, parallel design, randomised-controlled trial. Recruitment, interventions and assessments will be remote, enabling UK-wide participant involvement. 198 participants aged 18 years or more will be recruited via social media advertisement or via primary care physicians. A screening process will identify those with tinnitus that impacts health-related quality of life, and following consent smartphone-based audiometry will be performed. Randomisation 1:1 to the Oto app or one-to-one therapist-led tinnitus therapy will be performed centrally by computer, matching groups for age, sex and hearing level. Following participant allocation, the Oto app will be provided for immediate use, or a one-to-one remote therapy appointment booked to occur within approximately 1 week, with up to 6 sessions delivered. Participant outcomes will be collected at 4,12, 26 and 52 weeks via questionnaire and phone call. The primary outcome is the change in Tinnitus Functional Index (TFI) total score measured at 26 weeks following allocation. Adverse events will be recorded. A health economic evaluation in the form of a cost-utility analysis will be performed using data from participant submitted EuroQol 5D-5L and Health Utilities Index Mark 3 scores and resource use data. Trial results will be made publicly available, including a plain English summary

Physical properties and small-scale structure of the Lyman-alpha forest: Inversion of the HE 1122-1628 UVES spectrum

We study the physical properties of the Lyman-alpha forest by applying the inversion method described by Pichon et al. (2001) to the high resolution and high S/N ratio spectrum of the z_em=2.40 quasar HE 1122-1628 obtained during Science Verification of UVES at the VLT. We compare the column densities obtained with the new fitting procedure with those derived using standard Voigt profile methods. The agreement is good and gives confidence in the new description of the Lyman-alpha forest as a continuous field as derived from our method. We show that the observed number density of lines with logN>13 and 14 is, respectively, 50 and 250 per unit redshift at z~2. We study the physical state of the gas, neglecting peculiar velocities, assuming a relation between the overdensity and the temperature. T=Tbar * rho^(2beta). There is an intrinsic degeneracy between the parameters beta and Tbar. We demonstrate that, at a fixed beta, the temperature at mean density, Tbar, can be uniquely extracted however. While applying the method to HE 1122-1628, we conclude that for 0.2<beta<0.3, 6000<Tbar<15000 K at z~2. We investigate the small scale structure of strong absorption lines using the information derived from the Lyman-beta, Lyman-gamma and Civ profiles. Introducing the Lyman-beta line in the fit allows us to reconstruct the density field up to rho~10 instead of 5 for the Lyman-alpha line only. There may be small velocity shifts ~10km.s^{-1} between the peaks in the Civ and Hi density profiles. Although the statistics is small, it seems that Civ/Hi and n_HI are anti-correlated. This could be a consequence of the high sensitivity of the Civ/Hi ratio to temperature. The presence of associated Ovi absorption, with similar profile, confirms that the gas is photo-ionized and at a temperature of T~10^5 K.Comment: 15 pages, 21 figures, accepted for publication in A&A. Quoted results from other papers in section 4.2 have been modifie

Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms

The diversity of arbuscular mycorrhizal (AM) fungi was investigated in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for 3 yr. The experimental treatments comprised: bare soil; monocultures of the nonmycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP). The extent of AM colonization, shoot biomass and nitrogen and phosphorus concentrations were also measured. The AM diversity was affected significantly by the floristic composition of the microcosms and shoot phosphorus concentration was positively correlated with AM diversity. The diversity of AM fungi in P. lanceolata decreased in the order: bare soil > C. flacca > 12 species > F. ovina. The unexpectedly high diversity in the bare soil and sedge monoculture likely reflects differences in the modes of colonization and sources of inoculum in these treatments compared with the assemblages containing established AM-compatible plants

Does tendering create travesties of justice?

Legal aid in criminal proceedings exists to avoid defendants being presented as victims of an overbearing state, which assumes that ‘the two sides have access to roughly equivalent resources and expertise’ (Young and Wall, 1996). Recent governments have however focussed on value for money, which ignores political debate about what actually constitutes value in specific circumstances. It is against this background that the Ministry of Justice released its consultation paper including proposals to introduce Price Competitive Tendering (PCT) in most criminal proceedings

1.3 mm Wavelength VLBI of Sagittarius A*: Detection of Time-Variable Emission on Event Horizon Scales

Sagittarius A*, the ~4 x 10^6 solar mass black hole candidate at the Galactic Center, can be studied on Schwarzschild radius scales with (sub)millimeter wavelength Very Long Baseline Interferometry (VLBI). We report on 1.3 mm wavelength observations of Sgr A* using a VLBI array consisting of the JCMT on Mauna Kea, the ARO/SMT on Mt. Graham in Arizona, and two telescopes of the CARMA array at Cedar Flat in California. Both Sgr A* and the quasar calibrator 1924-292 were observed over three consecutive nights, and both sources were clearly detected on all baselines. For the first time, we are able to extract 1.3 mm VLBI interferometer phase information on Sgr A* through measurement of closure phase on the triangle of baselines. On the third night of observing, the correlated flux density of Sgr A* on all VLBI baselines increased relative to the first two nights, providing strong evidence for time-variable change on scales of a few Schwarzschild radii. These results suggest that future VLBI observations with greater sensitivity and additional baselines will play a valuable role in determining the structure of emission near the event horizon of Sgr A*.Comment: 8 pages, submitted to ApJ