11 research outputs found
Study of biological determinants of chlordecone transfer in the plant
Lâobjectif de cette thĂšse est de comprendre pourquoi certaines plantes sont en mesure dâabsorber plus de polluants organiques que dâautres. Il sâagit dâapprĂ©hender les paramĂštres qui, Ă lâĂ©chelle de la plante, rĂ©gissent lâabsorption dans les racines puis le transfert vers les parties aĂ©riennes. La chlordĂ©cone a Ă©tĂ© retenue comme molĂ©cule dâĂ©tude, pour ses caractĂ©ristiques physico-chimiques propices Ă lâĂ©tude des transferts sol-plante (faible volatilitĂ© et relative hydrophobicitĂ©) mais Ă©galement Ă cause des enjeux forts autour des sols contaminĂ©s dans les Antilles Françaises. Dans un premier temps, nous avons dĂ©cidĂ© de mettre en relation les processus liĂ©s Ă la croissance de la plante avec la capacitĂ© Ă accumuler la chlordĂ©cone lors dâessais en laboratoire. Des graminĂ©es modĂšles, comme le blĂ© ou le miscanthus, nous ont permis dâidentifier les traits biologiques impliquĂ©s dans lâabsorption puis la translocation de la molĂ©cule dans les diffĂ©rentes parties de la plante. A lâissue de cette premiĂšre phase, lâefficience de la transpiration, qui traduit Ă©galement la capacitĂ© de la plante Ă utiliser lâeau, est apparue comme le principal dĂ©terminant de la concentration de la chlordĂ©cone dans la plante. Dans un second temps, nous avons cherchĂ© Ă Ă©valuer le potentiel de contamination de la flore spontanĂ©e des parcelles contaminĂ©es de Guadeloupe, Ă travers un Ă©chantillonnage stratifiĂ©, câest-Ă -dire orientĂ© par des connaissances Ă priori ou par certains dĂ©terminants mis en Ă©vidence lors de la phase de laboratoire. Dans 70% des cas, lâanalyse de cette flore spontanĂ©e nous a permis de mettre en Ă©vidence le risque significatif de dĂ©passement des seuils rĂ©glementaires dans la viande animale nourrie avec la couverture vĂ©gĂ©tale des parcelles contaminĂ©es. Des pratiques culturales, comme lâintensification du travail du sol, sont Ă©galement apparues comme Ă©tant significativement corrĂ©lĂ©es Ă lâaugmentation des quantitĂ©s de chlordĂ©cone retrouvĂ©es dans la plante.The objective of this thesis is to understand the reasons why some plants are able to absorb more organic pollutants than others. The aim is to understand the parameters that, at the plant level, control the penetration into the roots and then the transfer to the aerial parts. Chlordecone was selected as a study molecule for its physico-chemical characteristics suitable for the study of soil-plant transfers (lowvolatility and relative hydrophobicity) but also because of the high stakes around contaminated soils in the FrenchWest Indies. Initially, we decided to relate the processes linked to plant growth to the capacity to accumulate chlordecone during laboratory tests. Model grasses, such as wheat ormiscanthus, allowed us to identify the biological traits involved in the absorption and then translocation of the molecule in the different parts of the plant. At the end of this first phase, the efficiency of transpiration, which also reflects the plantâs ability to use water, emerged as the main determinant of the concentration of chlordecone in the plant. In a second phase, we sought to assess the potential for contamination of the spontaneous flora of the contaminated fields in Guadeloupe, through stratified sampling, i.e. sampling oriented by a priori knowledge or by certain determinants highlighted during the laboratory phase. In 70% of the cases, the analysis of this spontaneous flora enabled us to highlight the significant risk of exceeding regulatory thresholds in animal meat fed with the plant cover of the contaminated plots. Cultivation practices, such as intensified tillage, also appeared to be significantly correlated with the increase in the amount of chlordecone found in the plant
Ătude des dĂ©terminants biologiques de l'absorption de la chlordĂ©cone par la plante
The objective of this thesis is to understand the reasons why some plants are able to absorb more organic pollutants than others. The aim is to understand the parameters that, at the plant level, control the penetration into the roots and then the transfer to the aerial parts. Chlordecone was selected as a study molecule for its physico-chemical characteristics suitable for the study of soil-plant transfers (lowvolatility and relative hydrophobicity) but also because of the high stakes around contaminated soils in the FrenchWest Indies. Initially, we decided to relate the processes linked to plant growth to the capacity to accumulate chlordecone during laboratory tests. Model grasses, such as wheat ormiscanthus, allowed us to identify the biological traits involved in the absorption and then translocation of the molecule in the different parts of the plant. At the end of this first phase, the efficiency of transpiration, which also reflects the plantâs ability to use water, emerged as the main determinant of the concentration of chlordecone in the plant. In a second phase, we sought to assess the potential for contamination of the spontaneous flora of the contaminated fields in Guadeloupe, through stratified sampling, i.e. sampling oriented by a priori knowledge or by certain determinants highlighted during the laboratory phase. In 70% of the cases, the analysis of this spontaneous flora enabled us to highlight the significant risk of exceeding regulatory thresholds in animal meat fed with the plant cover of the contaminated plots. Cultivation practices, such as intensified tillage, also appeared to be significantly correlated with the increase in the amount of chlordecone found in the plant.Lâobjectif de cette thĂšse est de comprendre pourquoi certaines plantes sont en mesure dâabsorber plus de polluants organiques que dâautres. Il sâagit dâapprĂ©hender les paramĂštres qui, Ă lâĂ©chelle de la plante, rĂ©gissent lâabsorption dans les racines puis le transfert vers les parties aĂ©riennes. La chlordĂ©cone a Ă©tĂ© retenue comme molĂ©cule dâĂ©tude, pour ses caractĂ©ristiques physico-chimiques propices Ă lâĂ©tude des transferts sol-plante (faible volatilitĂ© et relative hydrophobicitĂ©) mais Ă©galement Ă cause des enjeux forts autour des sols contaminĂ©s dans les Antilles Françaises. Dans un premier temps, nous avons dĂ©cidĂ© de mettre en relation les processus liĂ©s Ă la croissance de la plante avec la capacitĂ© Ă accumuler la chlordĂ©cone lors dâessais en laboratoire. Des graminĂ©es modĂšles, comme le blĂ© ou le miscanthus, nous ont permis dâidentifier les traits biologiques impliquĂ©s dans lâabsorption puis la translocation de la molĂ©cule dans les diffĂ©rentes parties de la plante. A lâissue de cette premiĂšre phase, lâefficience de la transpiration, qui traduit Ă©galement la capacitĂ© de la plante Ă utiliser lâeau, est apparue comme le principal dĂ©terminant de la concentration de la chlordĂ©cone dans la plante. Dans un second temps, nous avons cherchĂ© Ă Ă©valuer le potentiel de contamination de la flore spontanĂ©e des parcelles contaminĂ©es de Guadeloupe, Ă travers un Ă©chantillonnage stratifiĂ©, câest-Ă -dire orientĂ© par des connaissances Ă priori ou par certains dĂ©terminants mis en Ă©vidence lors de la phase de laboratoire. Dans 70% des cas, lâanalyse de cette flore spontanĂ©e nous a permis de mettre en Ă©vidence le risque significatif de dĂ©passement des seuils rĂ©glementaires dans la viande animale nourrie avec la couverture vĂ©gĂ©tale des parcelles contaminĂ©es. Des pratiques culturales, comme lâintensification du travail du sol, sont Ă©galement apparues comme Ă©tant significativement corrĂ©lĂ©es Ă lâaugmentation des quantitĂ©s de chlordĂ©cone retrouvĂ©es dans la plante
Growth parameters influencing uptake of chlordecone by Miscanthus species
Because of its high persistence in soils, t1/2 = 30 years, chlordecone (CLD) was classified as a persistent organic pollutant (POP) by the Stockholm Convention in 2009.The distribution of CLD over time has been heterogeneous, ranging from banana plantations to watersheds, and contaminating all environmental compartments. The aims of this study were to (i) evaluate the potential of Miscanthus species to extract chlordecone from contaminated soils, (ii) identify the growth parameters that influence the transfer of CLD from the soil to aboveground plant parts. CLD uptake was investigated in two species of Miscanthus, C4 plants adapted to tropical climates. M. sinensis and M. Ă giganteus were transplanted in a soil spiked with [14C]CLD at environmental concentrations (1 mg kgâ 1) under controlled conditions. Root-shoot transfer of CLD was compared in the two species after two growing periods (2 then 6 months) after transplantation. CLD was found in all plant organs, roots, rhizomes, stems, leaves, and even flower spikes. The highest concentration of CLD was in the roots, 5398 ± 1636 (M. Ă giganteus) and 14842 ± 3210 ng gâ 1 DW (M. sinensis), whereas the concentration in shoots was lower, 152 ± 28 (M. Ă giganteus) and 266 ± 70 ng gâ 1 DW (M. sinensis) in soil contaminated at 1 mg kgâ 1. CLD translocation led to an acropetal gradient from the bottom to the top of the plants. CLD concentrations were also monitored over two complete growing periods (10 months) in M. sinensis grown in 8.05 mg kgâ 1 CLD contaminated soils. Concentrations decreased in M. sinensis shoots after the second growth period due to the increase in organic matters in the vicinity of the roots. Results showed that, owing to their respective biomass production, the two species were equally efficient at phytoextraction of CLD
A Bayesian network approach for the identification of relationships between drivers of chlordecone bioaccumulation in plants
International audiencePlants were sampled from four different types of chlordecone-contaminated land in Guadeloupe (West Indies). The objective was to investigate the importance of biological and agri-environmental parameters in the ability of plants to bioaccumulate chlordecone. Among the plant traits studied, only the growth habit significantly affected chlordecone transfer, since prostrate plants concentrated more chlordecone than erect plants. In addition, intensification of land use has led to a significant increase in the amount of chlordecone absorbed by plants. The use of Bayesian networks uncovers some hypothesis and identifies paths for reflection and possible studies to identify and quantify relationships that explain our data
Database of metal concentrations measured in sediment cores (1965-2011) and bed and flood sediments (1987-2018) along the RhĂŽne River
This database contains metal element concentrations from (1) river sediment cores and (2) bed and flood deposits acquired along the RhÎne River by research teams (for 1: LEHNA-IPE) and monitoring programmes (for 2 : RMC Water Agency). This dataset focuses on six areas (geographical clusters: GC-1 to GC-6) located along the RhÎne River corridor (ca. 512 km), after its confluence with the Arve River (near Geneva in Switzerland) to its delta near Arles (France). It presents the variations of metal concentrations for the following elements: Al, Fe, Cd, Cr, Cu, Ni, Pb, Zn during the period 1965-2018. It also includes the geo-accumulation values (Igeo) and enrichment factors (EFs) for Cd, Cr, Cu, Ni, Pb, and Zn. Extraction techniques (Aqua Regia or Total Extraction) and ancillary data such as total organic carbon content (TOC in % - pct), grainsize (fine fraction proportion: clay and silts < 63 ”m), distance upstream of estuarine area (km UEA), sampling depth and date or expected age (for river sediment cores) are also reported
Metal pollution trajectories and mixture risk assessed by combining dated cores and subsurface sediments along a major European river (RhĂŽne River, France)
International audienceIn European rivers, research and monitoring programmes have targeted metal pollution from bed and floodplain sediments since the mid-20th century by using various sampling and analysis protocols. We propose to characterise metal contamination trajectories since the 1960s based on the joint use of a large amount of data from dated cores and subsurface sediments along the RhoÌne River (ca. 512 km, SwitzerlandâFrance). For the reconstruction of spatio-temporal trends, enrichment factors (EF) and geo-accumulation (Igeo) approaches were compared. The latter index was preferred due to the recurrent lack of grain-size and lithogenic elements in the dataset. Local geochemical backgrounds were established near (1) the Subalps and (2) the Massif Central to consider the geological variability of the watershed. A high contamination (Igeo = 3â5) was found for Cd, Cu and Zn from upstream to downstream over the period 1980â2000. This pattern is consistent with long-term emissions from major cities and the nearby industrial areas of the Upper RhoÌne (Geneva, Arve Valley), and Middle RhoÌne (Lyon, Chemical Corridor, Gier Valley). Hotspots due to Cu and Zn leaching from vineyards, mining, and highway runoff were also identified, while Pb was especially driven by industrial sources. The recovery time of pollution in sediment varied according to the metals and was shorter upstream of Lyon (15â20 years) than downstream (30â40 years). More widely, it was faster on the RhoÌne than along other European rivers (e.g. Seine and Rhine). Finally, the ecotoxicological mixture risk of metal with Persistent Organic Pollutants (POPs) for sediment-dwelling organisms showed a medium âcocktail riskâ dominated by metals upstream of Lyon, although it is enhanced due to POPs downstream, and southward to the delta and the Mediterranean Sea. Overall, this study demonstrates the heterogeneity of the contamination trends along large fluvial corridors such as the RhoÌne River
Evolution de la pollution métallique et du risque écotoxicologique le long du RhÎne depuis 1960 : approche combinée à partir du suivi particulaire et d'archives sédimentaires datées
International audienc
Evolution de la pollution métallique et du risque écotoxicologique le long du RhÎne depuis 1960 : approche combinée à partir du suivi particulaire et d'archives sédimentaires datées
International audienc
Evolution de la pollution métallique et du risque écotoxicologique le long du RhÎne depuis 1960 : approche combinée à partir du suivi particulaire et d'archives sédimentaires datées
International audienc
Evolution de la pollution métallique et du risque écotoxicologique le long du RhÎne depuis 1960 : approche combinée à partir du suivi particulaire et d'archives sédimentaires datées
International audienc