"Comprehensive Care" Concept in Nursing: Systematic Review.

Introduction. Integrated health care is a concept widely used in the planning and organisation of nursing care. It is a highly topical concept, but at the same time it is deeply rooted in the theory and models of Nursing right from its inception as a science. There is no clear, agreed definition that describes it. Objective. To systematise the knowledge available on the concept of "comprehensive care" in Nursing from the point of view of nursing care, its domains and characteristics. Methods. A literature search has been carried out in several languages (Spanish, Portuguese, English and Romanian) in the databases Web of Science, Scopus, Medline, PubMed, Cochrane and Dialnet, covering the period between 2013 and 2019. The search terms used were: comprehensive health care, health and nursing. Prospero register 170327. Results. Sixteen documents were identified, which grouped 8 countries, mainly Brazil, being the country with the highest output on this context– 10 documents were found within the qualitative paradigm and 6 quantitative ones. The concept "Comprehensive" ; is commonly used to refer to comprehensive nursing care techniques, protocols, programmes and plans, covering care in all aspects of the individual as a complement to or independent of the clinical needs arising from health care. Conclusion. The definition of features pertaining to the concept "Comprehensive Care" ; encourages the use and standardisation of nursing care plans, improving patient follow-up, the detection of new risk factors, complications and new health problems not related to the reason for admission. This increases the capacity for prevention and improves the patient’s quality of life, and their primary and/or family caregivers, which translates into lower costs in the health system.post-print871 K

The Bajo Segura Basin (SE Spain): implications for the Messinian salinity crisis in the Mediterranean margins

The analysis of the Messinian and Pliocene stratigraphy of the Bajo Segura Basin (a marginal basin of the western Mediterranean) has revealed three synthems deposited in a high sea-level context: T-MI (late Tortonian-Messinian), MII (Messinian), and P (early Pliocene), bounded by two lowstand erosional surfaces (intra-Messinian and end-Messinian unconformities). With respect to the salinity crisis, we propose the following series of events: 1) pre-evaporitic or pre-crisis phase (T-MI synthem); 2) first sea-level fall and subaerial exposure (intra-Messinian unconformity), possibly related to the precipitation of the Lower Evaporites; 3) syn-evaporitic phase (MII synthem), recorded both by selenitic gypsum (Upper Evaporites) as well as by lagoon deposits (Lago-Mare); 4) second sea-level fall and subaerial exposure (end-Messinian unconformity), characterized by deeply incised palaeovalleys; and 5) postevaporitic or post-crisis phase (P synthem), which coincides with the definitive restoration of open marine conditions in the basin. A combined biostratigraphic and magnetostratigraphic study revealed that all the events linked to the salinity crisis (from the end of the pre-evaporitic phase to the beginning of the post-evaporitic phase) occurred within the chron C3r (c. 5.9-5.2 Ma).Financial aid was provided by Research Projects BTE2003-05047, CGL2005-06224BTE (MEC), CGL2007-65832/BTE (MEC), GV04B-629 (Generalitat Valenciana) and “Paleoenvironmental Changes” Group (UA)

Assisted protein folding at low temperature: evolutionaryadaptation of the Antarctic fish chaperonin CCT and its clientproteins

Eukaryotic ectotherms of the Southern Ocean face energetic challenges to protein folding assisted by the cytosolic chaperonin CCT. We hypothesize that CCT and its client proteins (CPs) have co-evolved molecular adaptations that facilitate CCT–CP interaction and the ATP-driven folding cycle at low temperature. To test this hypothesis, we compared the functional and structural properties of CCT–CP systems from testis tissues of an Antarctic fish, Gobionotothen gibberifrons (Lo¨nnberg) (habitat/body T=-1.9 to +2˚C), and of the cow (body T=37˚C). We examined the temperature dependence of the binding of denatured CPs (bactin, b-tubulin) by fish and bovine CCTs, both in homologous and heterologous combinations and at temperatures between 24˚C and 20˚C, in a buffer conducive to binding of the denatured CP to the open conformation of CCT. In homologous combination, the percentage of G. gibberifrons CCT bound to CP declined linearly with increasing temperature, whereas the converse was true for bovine CCT. Binding of CCT to heterologous CPs was low, irrespective of temperature. When reactions were supplemented with ATP, G. gibberifrons CCT catalyzed the folding and release of actin at 2˚C. The ATPase activity of apo-CCT from G. gibberifrons at 4˚C was, 2.5-fold greater than that of apo-bovine CCT, whereas equivalent activities were observed at 20˚C. Based on these results, we conclude that the catalytic folding cycle of CCT from Antarctic fishes is partially compensated at their habitat temperature, probably by means of enhanced CP-binding affinity and increased flexibility of the CCT subunits

The 8.2-event record on the Alicante marine continental shelf (SE, Spain)

High resolution seismic profiles of the Alicante continental shelf have been studied identifying a seismic prism which top at about -20 m below today sea-level. The prism is covered by recent sediments and can be interpreted as formed during a short interval of stand-by in the general transgression after the last glacial maximum. The -20 m level have been compared with the holocene Mediterranean sea-level-curve to propose an age of about 8 ka BP coinciding with the «8.2 ka cooling event» that was an abrupt, widespread climate instability. The prism top is deeper in the northern seismic profiles thus indicating a more subsidence that the southern coastal shelf where an erosion surface with rocky shoals configure the sea botto

Isla de Nueva Tabarca

Geolodía surge en el año 2005 de una iniciativa de José Luis Simón, de la Universidad de Zaragoza, y de Luis Alcalá, de la Fundación Dinópolis. El “Geolodía” es convocado desde el año 2010 por la Sociedad Geológica de España, la Asociación Española Para la Enseñanza de las Ciencias de la Tierra y el Instituto Geológico y Minero de España. Esta actividad pretende acercar a la sociedad tanto la Geología como la profesión de geólogo a partir de itinerarios didácticos guiados por expertos, en lugares interesantes por su entorno geológico. Además de una divulgación rigurosa, pretende sensibilizar a la población sobre la importancia y necesidad de proteger nuestro patrimonio geológico. Este año celebramos la décima edición de Geolodía en la provincia de Alicante. La participación de 3000 personas en la pasada edición de 2016 en Agosto, nos ha forzado a realizar inscripción gratuita, y limitar la participación. También hemos suprimido la ruta familiar y los talleres. Pero estamos diseñando una actividad específica dirigida a este tipo de público que esperamos ofertar en estos próximos años. En esta edición hemos aprovechado una de las maravillas naturales de la provincia de Alicante, la isla de Nueva Tabarca, para ofrecer una ruta de 5 kilómetros que discurre por su perímetro.Instituto Geológico y Minero de España, EspañaPeer reviewe

C3 glomerulopathy-associated CFHR1 mutation alters FHR oligomerization and complement regulation

C3 glomerulopathies (C3G) are a group of severe renal diseases with distinct patterns of glomerular inflammation and C3 deposition caused by complement dysregulation. Here we report the identification of a familial C3G-associated genomic mutation in the gene complement factor H–related 1 (CFHR1), which encodes FHR1. The mutation resulted in the duplication of the N-terminal short consensus repeats (SCRs) that are conserved in FHR2 and FHR5. We determined that native FHR1, FHR2, and FHR5 circulate in plasma as homo- and hetero-oligomeric complexes, the formation of which is likely mediated by the conserved N-terminal domain. In mutant FHR1, duplication of the N-terminal domain resulted in the formation of unusually large multimeric FHR complexes that exhibited increased avidity for the FHR1 ligands C3b, iC3b, and C3dg and enhanced competition with complement factor H (FH) in surface plasmon resonance (SPR) studies and hemolytic assays. These data revealed that FHR1, FHR2, and FHR5 organize a combinatorial repertoire of oligomeric complexes and demonstrated that changes in FHR oligomerization influence the regulation of complement activation. In summary, our identification and characterization of a unique CFHR1 mutation provides insights into the biology of the FHRs and contributes to our understanding of the pathogenic mechanisms underlying C3G

Caracterización estructural de chaperoninas de tipo II mediante técnicas de criomicroscopía electrónica y cristalografía de rayos X

Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 30-03-2011Chaperonins, also known as Hsp60s, constitute an ubiquitously distributed family of molecular chaperones, found in all archaea, bacterya and eukaryotic organisms. They are macromolecular complexes composed of between 14-18 60 kDa subunits arranged in two identical rings stacked back to back. All chaperonins share several characteristics such as ATPase activity, domain structure of the subunits (with three distinct domains: equatorial, intermediate and apical), and a functional mechanism which couples ATP binding and hydrolysis to a coordinated conformational change which promotes the folding of substrate proteins. Chaperonins have been further classified in two distinct groups, I and II, based on significant structural and functional differences. Group I chaperonins, whose main representative is GroEL from E.coli, are found exclusively in all bacteria and endosymbiotic organelles such as mithochondria and chloroplasts. They are composed by two homoheptameric rings, and need the concourse of a cochaperonin, GroES in E.coli, which acts as a lid closing the cavity so that the protein could eventually fold using the information encoded in its aminoacid sequence. On the other hand, group II chaperonins are present in archaea, where they are known as thermosomes, and in the eukaryotic cytosol where a single member of this family is found, and it has been termed CCT (Chaperonin Containing TCP-1). Group II chaperonins are usually formed by two homooctameric or heterooctameric rings with a number of different subunits ranging from 1-3 in thermosomes to the 8 different subunits in the case of CCT. The main difference with group I chaperonins resides in the lack of a cochaperonin, whose function is replaced by an extended helical protrusion which closes the ring. Although there is a wide corpus of research regarding the structure and function of group I chaperonins, there is no so much information regarding group II chaperonins. In the present study we have attempted to obtain high resolution structural data of two of these chaperonins, an archaeal homo-oligomeric thermosome and the ecukaryotic cytosolic chaperonin CCT, with the aim of filling a long standing vaccum regarding the knowledge of these molecular chaperones. In the case of thermosome, we have developed a focal pairs-based cryoelectron microscopy approach aiming to determine a high resolution structure of the open conformation of an homo-oligomeric thermosome. This approach has not been completely succesful, but has served to identify several problems in the process which we believe must be useful in the future. In the case of CCT, it has been possible to obtain, using X-ray crystallography techniques, the only high resolution structure of the whole CCT chaperonin in complex with a substrate, tubulin, a breakthrough in the chaperonin research field that has allowed us to complete the structural and functional picture of this interesting molecular machine

Morphological and ichnological features of the boundary Messinian-Pliocene erosional surface (Sierra del Colmenar, Alicante). Relationship with the Mediterranean salinity crisis

The Messinian/Pliocene boundary in the Sierra del Colmenar (Alicante) is represented by an erosional surface called the end-Messinian unconformity. This erosional surface cuts the coastal and lagoonal deposits of the late Messinian (Terminal Complex) as a result of the sea-level fall related to the salinity crisis in the centre of the Mediterranean. The beginning of the subsequent transgresion during the lower Pliocene is registered by an erosive surface that carved the underlying Messinian deposits. This surface morphology and trace fossil colonization is controlled by the substrate characteristics. Thus, onto consistent calcareous substrates it is developed coastal palaeocliffs colonized by Trypanites icnofacies, meanwhile on the marly substrates there is no abrupt palaeocliffs with icnofacies of Skolithos colonization. The enhancing Pliocene reflooding was recorded by the sedimentation of coastal and shallow marine deposits over the erosive surface.Este trabajo se ha realizado en el marco de los proyectos de investigación BTE2003-05047 MCYT y GV04B-629

The Lago Mare-like sedimentation (Messinian) and the Pliocene transgression in Alicante (Bajo Segura Basin)

In the Alicante city and surrounding sectors the uppermost par t of the Messinian sequence (MII unit) is characterized by the stacking of shallowing upward cycles. The complete cycle star ts with beach sandstones and ends with lagoonal marls which contains ostracods (Cyprideis), benthic forams (dominated by the genus Ammonia) and chara algae. This fossil content is similar to that recorded in the well-known Lago Mare facies in the Mediterranean. The upper boundary of the MII unit is a subaerial erosional surface (end- Messinian unconformity) that shows paleovalley infilled by fluvial deposits and scree breccias (MIII unit). Over this erosional surface were deposited the Pliocene unit, which is composed by a lower system (P1) of marine marls enriched in planktonic fossils, and an upper system (P2) dominated by coastal and shallow marine clastic deposits. This Pliocene unit is the record of the marine flooding -Pliocene transgressionthat post-date the Lago Mare period in the Mediterranean.Este trabajo se ha realizado en el marco del proyecto de investigación BTE2003-05047 MCYT