Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review

The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported

Detection of Trypanosoma cruzi and Leishmania using the polymerase chain reaction

Submitted by sandra infurna ([email protected]) on 2016-06-29T15:15:58Z No. of bitstreams: 1 carlos19_morel_etal_IOC_1994.pdf: 132227 bytes, checksum: 7067101ca08673ccb5663ffa8bfac350 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-06-29T15:25:57Z (GMT) No. of bitstreams: 1 carlos19_morel_etal_IOC_1994.pdf: 132227 bytes, checksum: 7067101ca08673ccb5663ffa8bfac350 (MD5)Made available in DSpace on 2016-06-29T15:25:57Z (GMT). No. of bitstreams: 1 carlos19_morel_etal_IOC_1994.pdf: 132227 bytes, checksum: 7067101ca08673ccb5663ffa8bfac350 (MD5) Previous issue date: 1994Submitted by Angelo Silva ([email protected]) on 2016-07-07T11:16:53Z No. of bitstreams: 3 carlos19_morel_etal_IOC_1994.pdf.txt: 2 bytes, checksum: e1c06d85ae7b8b032bef47e42e4c08f9 (MD5) carlos19_morel_etal_IOC_1994.pdf: 132227 bytes, checksum: 7067101ca08673ccb5663ffa8bfac350 (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-07-07T12:12:34Z (GMT) No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) carlos19_morel_etal_IOC_1994.pdf: 132227 bytes, checksum: 7067101ca08673ccb5663ffa8bfac350 (MD5) carlos19_morel_etal_IOC_1994.pdf.txt: 2 bytes, checksum: e1c06d85ae7b8b032bef47e42e4c08f9 (MD5)Made available in DSpace on 2016-07-07T12:12:34Z (GMT). No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) carlos19_morel_etal_IOC_1994.pdf: 132227 bytes, checksum: 7067101ca08673ccb5663ffa8bfac350 (MD5) carlos19_morel_etal_IOC_1994.pdf.txt: 2 bytes, checksum: e1c06d85ae7b8b032bef47e42e4c08f9 (MD5) Previous issue date: 1994Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil

Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy

Thirty eight patients with indeterminate leprosy (HI), at least 4 to 6 years after discharge from multibacillary (MB) or paucibacillary (PB) schemes of anti leprosy multidrug therapy (MDT), were submitted to traditional diagnostic procedures for leprosy and to polymerase chain reaction (PCR) analysis of different clinical samples for detection of Mycobacterium leprae DNA. No significant difference was observed for any of the parameters analyzed between PB or MB schemes of treatment and no indications were found for more efficient outcome of HI using the MB scheme. Remarkably, 18 (54.5%) of the individuals were PCR positive in at least one of the samples: positivity of PCR was highest in blood samples and four individuals were PCR positive in blood and some other sample. Upon comparison of PCR results with clinical and histopathological parameters, no correlation was found between PCR-positivity and eventual relapse. This is the first report on detection of M. leprae DNA in PB patients, more than half a decade after completion of MDT, suggesting that live bacilli are present and circulating much longer than expected, although reinfection of the individuals can not be excluded. Overall, we feel that because of the high sensitivity of the assay, extreme care should be taken about association of PCR results, efficacy of treatment and disease status

Antigens of Trypanosoma cruzi with clinical interest cloned and expressed in Escherichia coli

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Antigens of Trypanosoma cruzi with clinical interest cloned and expressed in Escherichia coli

Escola Paulista de Medicina Disciplina de ParasitologiaBio-Merieux Laboratoire de Biologie CellulaireUSP Instituto de Medicina TropicalUFGo Faculdade de Medicina de GoiâniaUNICAMP Hospital das ClínicasHospital de Ninos Ricardo GutierrezFIOCRUZ Departamento de Biologia MolecularUNIFESP, EPM, Disciplina de ParasitologiaSciEL