Alien Registration- Humphrey, William E. (Knox, Waldo County)

https://digitalmaine.com/alien_docs/5173/thumbnail.jp

Serendipitous XMM-Newton discovery of a cluster of galaxies at z=0.28

We report the discovery of a galaxy cluster serendipitously detected as an extended X-ray source in an offset observation of the group NGC 5044. The cluster redshift, z=0.281, determined from the optical spectrum of the brightest cluster galaxy, agrees with that inferred from the X-ray spectrum using the Fe K alpha complex of the hot ICM (z=0.27 +/- 0.01). Based on the 50 ks XMM observation, we find that within a radius of 383 kpc the cluster has an unabsorbed X-ray flux, f_X (0.5-2 keV) = 3.34 (+0.08, -0.13) x 10^{-13} erg/cm^2/s, a bolometric X-ray luminosity, L_X = 2.21 (+0.34, -0.19) x 10^{44} erg/s, kT = 3.57 +/- 0.12 keV, and metallicity, 0.60 +/- 0.09 solar. The cluster obeys the scaling relations for L_X and T observed at intermediate redshift. The mass derived from an isothermal NFW model fit is, M_vir = 3.89 +/- 0.35 x 10^{14} solar masses, with a concentration parameter, c = 6.7 +/- 0.4, consistent with the range of values expected in the concordance cosmological model for relaxed clusters. The optical properties suggest this could be a ``fossil cluster''.Comment: 5 pages, 4 colour figures, accepted for publication in Ap

Bone marrow-derived mesenchymal stem cells become anti-angiogenic when chondrogenically or osteogenically differentiated:implications for bone and cartilage tissue engineering

Osteochondral tissue repair requires formation of vascularized bone and avascular cartilage. Mesenchymal stem cells stimulate angiogenesis both in vitro and in vivo but it is not known if these proangiogenic properties change as a result of chondrogenic or osteogenic differentiation. We investigated the angiogenic/antiangiogenic properties of equine bone marrow-derived mesenchymal stem cells (eBMSCs) before and after differentiation in vitro. Conditioned media from chondrogenic and osteogenic cell pellets and undifferentiated cells was applied to endothelial tube formation assays using Matrigel™. Additionally, the cell secretome was analysed using LC-MS/MS mass spectrometry and screened for angiogenesis and neurogenesis-related factors using protein arrays. Endothelial tube-like formation was supported by conditioned media from undifferentiated eBMSCs. Conversely, chondrogenic and osteogenic conditioned media was antiangiogenic as shown by significantly decreased length of endothelial tube-like structures and degree of branching compared to controls. Undifferentiated cells produced higher levels of angiogenesis-related proteins compared to chondrogenic and osteogenic pellets. In summary, eBMSCs produce an array of angiogenesis-related proteins and support angiogenesis in vitro via a paracrine mechanism. However, when these cells are differentiated chondrogenically or osteogenically, they produce a soluble factor(s) that inhibits angiogenesis. With respect to osteochondral tissue engineering, this may be beneficial for avascular articular cartilage formation but unfavourable for bone formation where a vascularized tissue is desired

Amplification of Black Vulture (\u3ci\u3eCoragyps atratus\u3c/i\u3e) DNA from regurgitated food pellets

Studies that rely on noninvasive collection of DNA for birds often use feces or feathers. Some birds, such as vultures, regurgitate undigested matter in the form of pellets that are commonly found under roost sites. Our research demonstrates that regurgitated pellets are a viable, noninvasive source of DNA for molecular ecology studies of vultures. Our objectives were to amplify 5 microsatellite loci designed for distinguishing Turkey Vultures (Cathartes aura) and Black Vultures (Coragyps atratus) in a single, multiplexed PCR, and to determine how long the target nuclear DNA persists after a vulture pellet is regurgitated and exposed to the environment. We collected pellets from captive Black Vultures and placed them in an outdoor aviary for a maximum estimated total of 12, 24, 36, or 48 h. We swabbed pellet surfaces for extraction and amplified vulture DNA using the panel of markers. All amplified alleles fell within predicted ranges of Black Vultures for all 5 loci, supporting the use of this microsatellite panel for vulture species identification. Overall amplification success for samples collected 0–12 h after regurgitation was 82.3%. Pellets collected 12–24 h, 24–36 h, and 36–48 h after regurgitation had only 18%, 10.2%, and 4.5% amplification success, respectively, which may have been due to a rain event. Our approach will be useful for noninvasive genetic sampling targeting nuclear DNA. These results should encourage noninvasive genetic sampling studies of other species that regurgitate pellets, such as raptors, water birds, or shorebirds

Detecting an Elusive Invasive Species: A Diagnostic PCR to Detect Burmese Python in Florida Waters and an Assessment of Persistence of Environmental DNA

Recent studies have demonstrated that detection of environmental DNA (eDNA) from aquatic vertebrates in water bodies is possible. The Burmese python, Python bivittatus, is a semi-aquatic, invasive species in Florida where its elusive nature and cryptic coloration make its detection difficult. Our goal was to develop a diagnostic PCR to detect P. bivittatus from water-borne eDNA, which could assist managers in monitoring this invasive species. First, we used captive P. bivittatus to determine whether reptilian DNA could be isolated and amplified from water samples. We also evaluated the efficacy of two DNA isolation methods and two DNA extraction kits commonly used in eDNA preparation. A fragment of the mitochondrial cytochrome b gene from P. bivittatus was detected in all water samples isolated with the sodium acetate precipitate and the QIAamp DNA Micro Kit. Next, we designed P. bivittatus-specific primers and assessed the degradation rate of eDNA in water. Our primers did not amplify DNA from closely related species, and we found that P. bivittatus DNA was consistently detectable up to 96 h. Finally, we sampled water from six field sites in south Florida. Samples from five sites, where P. bivittatus has been observed, tested positive for eDNA. The final site was negative and had no prior documented evidence of P. bivittatus. This study shows P. bivittatus eDNA can be isolated from water samples; thus, this method is a new and promising technique for the management of invasive reptiles

Telescoped approach to aryl hydroxymethylation in the synthesis of a key pharmaceutical intermediate

An efficient synthetic approach leading to introduction of the hydroxymethyl group to an aryl moiety via combination of the Bouveault formylation and hydride reduction has been optimized using a rational, mechanistic-based approach. This approach enabled telescoping of the two steps into a single efficient process, readily amenable to scaleup

Conspecific chemical cues facilitate mate trailing by invasive Argentine black and white tegus

Squamate reptiles (snakes and lizards) rely on chemical cues from conspecifics to search the environment for potential mates. How such cues are used by invasive species to facilitate reproduction, especially seasonally, is a key question that can inform management practices. The Argentine black and white tegu (Salvator merianae) is an invasive reptile species in south Florida threatening native fauna in biodiverse regions such as Everglades National Park. While some information exists on the reproductive ecology of this species in its native range in South America, the chemical ecology of S. merianae is unclear especially in its invasive range. By testing both male (n = 7) and female (n = 7) tegus in a Y-maze apparatus, we assessed if either sex follows chemical trails left by conspecifics and if behaviors were sex- or season-specific. We conducted three types of trials where conspecifics created odor trails: Male-only (male scent only in base and one arm of Y), Female-only, and Male vs. female. Males did not preferentially follow scent trails from either sex, but they did differentially investigate conspecific scent from both sexes. Seasonally, males showed increased rates of chemosensory sampling (rates of tongue-flicking) during the spring (breeding season; March-May) compared to fall (non-breeding season; September-November). Males also had reduced turning and pausing behavior while trailing in the spring. Female tegus exhibited stronger conspecific trailing abilities than males, following both male and female scent trails, and they explored the maze less before making an arm choice. Females also investigated the scent trails intensely compared to males (more passes in scented arms, more time with scent trails). Our results demonstrate for the first time that females of an invasive reptile species can follow conspecific scent trails. Given the strong female responses to odor, sex-specific targeting of tegus via application of a conspecific chemical cue in traps could enhance removal rates of females during the breeding season

Nanoscale thermal transport. II. 2003–2012

A diverse spectrum of technology drivers such as improved thermal barriers, higher efficiency thermoelectric energy conversion, phase-change memory, heat-assisted magnetic recording, thermal management of nanoscale electronics, and nanoparticles for thermal medical therapies are motivating studies of the applied physics of thermal transport at the nanoscale. This review emphasizes developments in experiment, theory, and computation in the past ten years and summarizes the present status of the field. Interfaces become increasingly important on small length scales. Research during the past decade has extended studies of interfaces between simple metals and inorganic crystals to interfaces with molecular materials and liquids with systematic control of interface chemistry and physics. At separations on the order of ~1 nm , the science of radiative transport through nanoscale gaps overlaps with thermal conduction by the coupling of electronic and vibrational excitations across weakly bonded or rough interfaces between materials. Major advances in the physics of phonons include first principles calculation of the phonon lifetimes of simple crystals and application of the predicted scattering rates in parameter-free calculations of the thermal conductivity. Progress in the control of thermal transport at the nanoscale is critical to continued advances in the density of information that can be stored in phase change memory devices and new generations of magnetic storage that will use highly localized heat sources to reduce the coercivity of magnetic media. Ultralow thermal conductivity—thermal conductivity below the conventionally predicted minimum thermal conductivity—has been observed in nanolaminates and disordered crystals with strong anisotropy. Advances in metrology by time-domain thermoreflectance have made measurements of the thermal conductivity of a thin layer with micron-scale spatial resolution relatively routine. Scanning thermal microscopy and thermal analysis using proximal probes has achieved spatial resolution of 10 nm, temperature precision of 50 mK, sensitivity to heat flows of 10 pW, and the capability for thermal analysis of sub-femtogram samples.United States. Air Force Office of Scientific Research. Multidisciplinary University Research Initiative (FA9550-08-1-0407

The Kinematics of the Globular Cluster System of NGC 5128 with a New, Large Sample of Radial Velocity Measurements

New radial velocity measurements for previously known and newly confirmed globular clusters (GCs) in the nearby massive galaxy NGC 5128 are presented. We have obtained spectroscopy from LDSS-2/Magellan, VIMOS/VLT, and Hydra/CTIO from which we have measured the radial velocities of 218 known, and identified 155 new, GCs. The current sample of confirmed GCs in NGC 5128 is now 605 with 564 of these having radial velocity measurements. We have performed a new kinematic analysis of the GC system that extends out to 45 arcmin in galactocentric radius. We have examined the systemic velocity, projected rotation amplitude and axis, and the projected velocity dispersion of the GCs as functions of galactocentric distance and metallicity. Our results indicate that the metal-poor GCs have a very mild rotation signature of (26 pm 15) km/s. The metal-rich GCs are rotating with a higher, though still small signature of (43 pm 15) km/s around the isophotal major axis of NGC 5128 within 15 arcmin. Their velocity dispersions are consistent within the uncertainties and the profiles appear flat or declining within 20 arcmin. We note the small sample of metal-rich GCs with ages less than 5 Gyr in the literature appear to have different kinematic properties than the old, metal-rich GC subpopulation. The mass and mass-to-light ratios have also been estimated using the GCs as tracer particles for NGC 5128. Out to a distance of 20 arcmin, we have obtained a mass of (5.9 pm 2.0) x 10^(11) M_(sun) and a mass-to-light ratio in the B-band of 16 M_(sun)/L_(B,sun). Combined with previous work on the ages and metallicities of its GCs, as well as properties of its stellar halo, our findings suggest NGC 5128 formed via hierarchical merging over other methods of formation, such as major merging at late times.Comment: Accepted for The Astronomical Journal, 14 pages plus 12 figures and 7 table

Colloquium on Solid State Devices

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