Sainfoin – New Data on Anthelmintic Effects and Production in Sheep and Goats

Gastrointestinal nematodes (GIN) are one of the most important problems affecting health and therefore performance and welfare in small ruminant husbandry. The control of these parasites in the past strongly relied on the repeated use of anthelmintic drugs. This has led to nematode populations which are resistant to most of the currently available anthelmintics. Furthermore customer’s demands for organic and residue free animal products are increasing. The aforementioned problems have given a strong impetus for the development of new non-chemical strategies to control GIN. Previous research has pointed out the anthelmintic potential of sainfoin (Onobrychis viciifolia) and other tanniferous (CT) feed sources in goats and lambs infected with GIN. A recent Swiss experiment focussed on the use of sainfoin and field bean (Vicia faba, cv. Scirocco) as single CT sources as well as in combination for additional synergic effects, to reduce periparturient GIN egg rise of ewes in late gestation and early lactation. Another experiment with Alpine goats concentrated on the influence of sainfoin on milk performance and cheese quality. The results of these experiments will be presented and discussed in connection with previous knowledge on (i) anthelmintic effects of sainfoin and (ii) the influence of sainfoin administration on performance

Entropy production and Lyapunov instability at the onset of turbulent convection

Computer simulations of a compressible fluid, convecting heat in two dimensions, suggest that, within a range of Rayleigh numbers, two distinctly different, but stable, time-dependent flow morphologies are possible. The simpler of the flows has two characteristic frequencies: the rotation frequency of the convecting rolls, and the vertical oscillation frequency of the rolls. Observables, such as the heat flux, have a simple-periodic (harmonic) time dependence. The more complex flow has at least one additional characteristic frequency -- the horizontal frequency of the cold, downward- and the warm, upward-flowing plumes. Observables of this latter flow have a broadband frequency distribution. The two flow morphologies, at the same Rayleigh number, have different rates of entropy production and different Lyapunov exponents. The simpler "harmonic" flow transports more heat (produces entropy at a greater rate), whereas the more complex "chaotic" flow has a larger maximum Lyapunov exponent (corresponding to a larger rate of phase-space information loss). A linear combination of these two rates is invariant for the two flow morphologies over the entire range of Rayleigh numbers for which the flows coexist, suggesting a relation between the two rates near the onset of convective turbulence.Comment: 5 pages, 4 figure

The energy budget in Rayleigh-Benard convection

It is shown using three series of Rayleigh number simulations of varying aspect ratio AR and Prandtl number Pr that the normalized dissipation at the wall, while significantly greater than 1, approaches a constant dependent upon AR and Pr. It is also found that the peak velocity, not the mean square velocity, obeys the experimental scaling of Ra^{0.5}. The scaling of the mean square velocity is closer to Ra^{0.46}, which is shown to be consistent with experimental measurements and the numerical results for the scaling of Nu and the temperature if there are strong correlations between the velocity and temperature.Comment: 5 pages, 3 figures, new version 13 Mar, 200

Use of 16S rRNA Gene Based Clone Libraries to Assess Microbial Communities Potentially Involved in Anaerobic Methane Oxidation in a Mediterranean Cold Seep

This study provides data on the diversities of bacterial and archaeal communities in an active methane seep at the Kazan mud volcano in the deep Eastern Mediterranean sea. Layers of varying depths in the Kazan sediments were investigated in terms of (1) chemical parameters and (2) DNA-based microbial population structures. The latter was accomplished by analyzing the sequences of directly amplified 16S rRNA genes, resulting in the phylogenetic analysis of the prokaryotic communities. Sequences of organisms potentially associated with processes such as anaerobic methane oxidation and sulfate reduction were thus identified. Overall, the sediment layers revealed the presence of sequences of quite diverse bacterial and archaeal communities, which varied considerably with depth. Dominant types revealed in these communities are known as key organisms involved in the following processes: (1) anaerobic methane oxidation and sulfate reduction, (2) sulfide oxidation, and (3) a range of (aerobic) heterotrophic processes. In the communities in the lowest sediment layer sampled (22–34 cm), sulfate-reducing bacteria and archaea of the ANME-2 cluster (likely involved in anaerobic methane oxidation) were prevalent, whereas heterotrophic organisms abounded in the top sediment layer (0–6 cm). Communities in the middle layer (6–22 cm) contained organisms that could be linked to either of the aforementioned processes. We discuss how these phylogeny (sequence)-based findings can support the ongoing molecular work aimed at unraveling both the functioning and the functional diversities of the communities under study

An interlaboratory study of TEX86 and BIT analysis of sediments, extracts and standard mixtures.

Two commonly used proxies based on the distribution of glycerol dialkyl glycerol tetraethers (GDGTs) are the TEX86 (TetraEther indeX of 86 carbon atoms) paleothermometer for sea surface temperature reconstructions and the BIT (Branched Isoprenoid Tetraether) index for reconstructing soil organic matter input to the ocean. An initial round-robin study of two sediment extracts, in which 15 laboratories participated, showed relatively consistent TEX86 values (reproducibility ±3-4°C when translated to temperature) but a large spread in BIT measurements (reproducibility ±0.41 on a scale of 0-1). Here we report results of a second round-robin study with 35 laboratories in which three sediments, one sediment extract, and two mixtures of pure, isolated GDGTs were analyzed. The results for TEX86 and BIT index showed improvement compared to the previous round-robin study. The reproducibility, indicating interlaboratory variation, of TEX86 values ranged from 1.3 to 3.0°C when translated to temperature. These results are similar to those of other temperature proxies used in paleoceanography. Comparison of the results obtained from one of the three sediments showed that TEX86 and BIT indices are not significantly affected by interlaboratory differences in sediment extraction techniques. BIT values of the sediments and extracts were at the extremes of the index with values close to 0 or 1, and showed good reproducibility (ranging from 0.013 to 0.042). However, the measured BIT values for the two GDGT mixtures, with known molar ratios of crenarchaeol and branched GDGTs, had intermediate BIT values and showed poor reproducibility and a large overestimation of the "true" (i.e., molar-based) BIT index. The latter is likely due to, among other factors, the higher mass spectrometric response of branched GDGTs compared to crenarchaeol, which also varies among mass spectrometers. Correction for this different mass spectrometric response showed a considerable improvement in the reproducibility of BIT index measurements among laboratories, as well as a substantially improved estimation of molar-based BIT values. This suggests that standard mixtures should be used in order to obtain consistent, and molar-based, BIT values

On the Long Lasting “C‐Type” Structures in the Sodium Lidargram: The Lifetime of Kelvin‐Helmholtz Billows in the Mesosphere and Lower Thermosphere Region

In order to understand the characteristics of long‐lasting “C‐type” structure in the Sodium (Na) lidargram, six cases from different observational locations have been analyzed. The Na lidargram, collected from low‐, middle‐, and high‐latitude sites, show long lifetime of the C‐type structures which is believed to be the manifestation of Kelvin‐Helmholtz (KH) billows in the Mesosphere and Lower Thermosphere (MLT) region. In order to explore the characteristics of the long‐lasting C‐type structures, the altitude profile of square of Brunt‐Väisälä frequency in the MLT region has been derived using the temperature profile collected from the Na lidar instruments and the SABER instrument onboard TIMED satellite. It is found to be positive in the C‐type structure region for all the six cases which indicates that the regions are convectively stable. Simultaneous wind measurements, which allowed us to calculate the Richardson numbers and Reynolds numbers for three cases, suggest that the regions where the C‐type structure appeared were dynamically stable and nonturbulent. This paper brings out a hypothesis wherein the low temperature can increase the magnitude of the Prandtl number and convectively stable atmospheric region can cause the magnitude of Reynolds number to decrease. As a consequence, the remnant of previously generated KH billows in nearly “frozen‐in” condition can be advected through this conducive region to a different location by the background wind where they can sustain for a long time without much deformation. These long‐lived KH billows in the MLT region will eventually manifest the long‐lasting C‐type structures in the Na lidargram

Integrated analysis of bacterial and microeukaryotic communities from differentially active mud volcanoes in the Gulf of Cadiz

The present study assesses the diversity and composition of sediment bacterial and microeukaryotic communities from deep-sea mud volcanoes (MVs) associated with strike-slip faults in the South-West Iberian Margin (SWIM). We used a 16S/18S rRNA gene based pyrosequencing approach to characterize and correlate the sediment bacterial and microeukaryotic communities from MVs with differing gas seep regimes and from an additional site with no apparent seeping activity. In general, our results showed significant compositional changes of bacterial and microeukaryotic communities in sampling sites with different seepage regimes. Sediment bacterial communities were enriched with Methylococcales (putative methanotrophs) but had lower abundances of Rhodospirillales, Nitrospirales and SAR202 in the more active MVs. Within microeukaryotic communities, members of the Lobosa (lobose amoebae) were enriched in more active MVs. We also showed a strong correlation between Methylococcales populations and lobose amoeba in active MVs. This study provides baseline information on the diversity and composition of bacterial and microeukaryotic communities in deep-sea MVs associated with strike-slip faults

Duddingtonia flagrans: a promising biocontrol agent for gastrointestinal nematodes

Controlling gastrointestinal nematodes (GIN) challenges owners of small ruminants with access to pastures. Biocontrol using the nematophagous fungus Duddingtonia flagrans is expected to complement existing alternatives for controlling GIN in grazing animals in the future. Animals receive spores of D. flagrans, which pass through the gastrointestinal tract. Fungal mycelium grows out of the surviving spores, spreads through the deposited faeces and forms structures with which it traps, colonizes and destroys the GIN larvae. This leads to reduced pasture contamination with GIN larvae and lower infection of grazing animals. An experiment with lactating goats, which were naturally infected with GIN, was carried out on an organic farm. Ten animals each received either a feed additive without D. flagrans spores (control), a feed additive with D. flagrans spores at recommended sheep/cattle dosage (normal) or a feed additive with D. flagrans spores at a10 times higher concentration than the normal dosage (high). Spores were administered daily to each animal individually during three days. Faecal samples were taken from all animals on the day before and on the last day of feeding spores. For each sample, faecal egg counts were determined by a modified McMaster technique. Samples were cultured for 14 days and larvae were subsequently obtained. Biocontrol efficacy of D. flagrans was calculated for each animal individually as a percentage reduction of developed larvae after treatment compared to the number obtained before treatment. As compared to the control, in the group with the normal D. flagrans dose infective larvae were reduced by about 20%, whereas reduction was almost 70% in the high D. flagrans dose group. In the H2020 project RELACS a similar setup was carried out with artificially infected sheep, using the normal and a 10 times lower dosage of D. flagrans spores. Infective GIN larvae were reduced by over 95% in faeces of both D. flagrans groups as compared to the control. Compared to goats, lower doses were required to substantially reduce GIN larval development in faecal cultures of sheep. Funded by EU H2020 No 773431 – RELACS

Integrated control of gastrointestinal nematodes in lambs using a bioactive feed x breed approach

Forages rich in condensed tannins have repeatedly shown potential to reduce gastrointestinal nematode infection in sheep. Similarly, several breeds of sheep have shown a relative resistance to gastrointestinal nematodes (GIN). We hypothesised that additive or even synergic effects may arise from the combination of a relatively resistant breed and a diet rich in condensed tannins. In study I, 160 lambs of the native Red Engadine Sheep (RES) and 113 lambs of the Swiss White Alpine sheep (SWA) were artificially infected with GIN and subsequently grazed for 52 days. The lambs were then distributed to 2 groups for a 14-day experimental feeding period. One group received a diet with a proportion of 55% sainfoin and was compared to a control group on the basis of faecal egg counts (FEC). In study II, 25 RES and 27 SWA lambs grazed infectious pastures for 37 days and were subsequently fed for 13 consecutive days with approximately 100% sainfoin or control forage. In addition to the FEC determination at the start and the end of the experimental feeding, the 52 lambs in study II were slaughtered and necropsied to determine their worm burden. FEC at the end of the feeding period were significantly lower in sainfoin fed lambs compared to controls in study I (p < 0.001) as well as in study II (p = 0.012). Breed, animal age, live weight, sex and the interaction of breed and treatment did not affect FEC in either study. The main nematode genera found in the sacrificed lambs of study II were Haemonchus spp., Teladorsagia spp., Nematodirus spp. and Trichostrongylus spp. Overall, RES had a significantly lower Haemonchus spp. (p = 0.035) and Trichostrongylus spp. (p = 0.003) worm burden compared to SWA. Regardless of breed, sainfoin feeding significantly reduced Teladorsagia spp. (p = 0.049) and Nematodirus spp. (p < 0.001) worm burden. Although, we could not demonstrate additive or synergic effects when using an integrated breed x sainfoin approach, the finding that a proportion of only 55% sainfoin in the diet led to a lower FEC compared to controls is important with respect to the implementation on producing farms. © 2013 Elsevier B.V. All rights reserved