SLIDR and SLOPPR : flexible identification of spliced leader trans-splicing and prediction of eukaryotic operons from RNA-Seq data

Acknowledgements The authors thank Bernadette Connolly for helpful discussions and Andreea Marin, David MacLeod and Lucrezia Piccicacchi for testing the pipelines. The authors acknowledge the support of the Maxwell and MacLeod computer clusters funded by the University of Aberdeen. Funding This work was supported by the Biotechnology and Biological Sciences Research Council [BB/J007137/1 to JP and BM, and BB/T002859/1 to BM and JP]. The funding body had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.Peer reviewedPublisher PD

A nematode-specific ribonucleoprotein complex mediates interactions between the major nematode spliced leader snRNP and its target pre-mRNAs

Acknowledgements We thank Maheshika Kurukulasuriya for her assistance with immunoprecipitations from embryo extracts. Some strains were provided by the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). Sequencing was performed by the Centre for Genome-Enabled Biology and Medicine of the University of Aberdeen, and proteomics analysis by the Aberdeen proteomics facility. We thank Kate Burgoyne and Craig Pattinson (Aberdeen Proteomics) for technical support. We thank WormBase for providing the community resource that facilitated the interrogation of C. elegans molecular genetics used in this work . Author contribution: P.E., M.A., E.S.-M., R.F., M.W., B.M. and J.P. contributed experiments. J.P. and B.M. conceived the research and managed and coordinated the research activity; J.P. and B.M. acquired funding for the project; D.S. guided the proteomics analysis, M.W., B.M. and J.P. designed and implemented the computational analysis; M.W., B.M. and J.P. wrote the manuscript and prepared figures and tables.Peer reviewe

A novel, essential trans-splicing protein connects the nematode SL1 snRNP to the CBC-ARS2 complex

Open Access via the OUP Agreement Some strains were provided by the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). Sequencing was performed by the Centre for Genome-Enabled Biology and Medicine of the University of Aberdeen, and proteomics analysis by the University of Aberdeen Proteomics facility. We thank WormBase for providing the community resource that facilitated the interrogation of C. elegans molecular genetics used in this work. FUNDING Biotechnology and Biological Sciences Research Council [BB/T002859/1]; EASTBIO Biotechnology and Biological Sciences Research Council PhD Studentship [BB/M010996/1 to R.E.B.S.]; University of Aberdeen Elphinstone PhD studentship (to R.Y.F.); University of Aberdeen. Funding for open access charge: Read and publish Agreement Scottish Institutions (SHEDL affiliated).Peer reviewedPublisher PD

Laser Patterning Pretreatment before Thermal Spraying: A Technique to Adapt and Control the Surface Topography to Thermomechanical Loading and Materials

Coating characteristics are highly dependent on substrate preparation and spray parameters. Hence, the surface must be adapted mechanically and physicochemically to favor coating–substrate adhesion. Conventional surface preparation methods such as grit blasting are limited by surface embrittlement and produce large plastic deformations throughout the surface, resulting in compressive stress and potential cracks. Among all such methods, laser patterning is suitable to prepare the surface of sensitive materials. No embedded grit particles can be observed, and high-quality coatings are obtained. Finally, laser surface patterning adapts the impacted surface, creating large anchoring area. Optimized surface topographies can then be elaborated according to the material as well as the application. The objective of this study is to compare the adhesive bond strength between two surface preparation methods, namely grit blasting and laser surface patterning, for two material couples used in aerospace applications: 2017 aluminum alloy and AISI 304L stainless steel coated with NiAl and YSZ, respectively. Laser patterning significantly increases adherence values for similar contact area due to mixed-mode (cohesive and adhesive) failure. The coating is locked in the pattern

20 Jahre Förderung von Strom aus Erneuerbaren Energien in Deutschland

20 JAHRE FÖRDERUNG VON STROM AUS ERNEUERBAREN ENERGIEN IN DEUTSCHLAND 20 Jahre Förderung von Strom aus Erneuerbaren Energien in Deutschland / Bruns, Elke (Rights reserved) ( -