Rebuilding Urban Place: Negotiating Individuality and Belonging

vi ABSTRACT Rebuilding Urban Place: Negotiating Individuality and Belonging New London, CT September 2013 PETER R. WEBSTER B.S.,TUFTS UNIVERSITY M.ARCH, UNIVERSITY OF MASSACHUSETTS, AMHERST Directed by: Professor Kathleen Lugosch The aim of this thesis is to test an approach for reconnecting ourselves with the urban fabric. By recognising the damage of urban renewal as more than simple blight but rather one that undermines our sense of place, we begin to appreciate the depth of the wound. It is not a matter of reconstructing what was taken away, but rather a process of rehabilitation. Re-establishing a viable sense of place requires the intertwining of both spatial form and social engagement. The project makes use of a parking lot located between the main street and a disruptive artery that forms a rift in the urban fabric. A spatial reorganization mediates the automotive scale of the rift and re-establishes a human one. A local organization, Hygienic Art, is poised to engage the rift with a new performing art center. Interactivity between the site and the client is reflected outward across the block and inward through the building. The center’s performance and service areas are designed to facilitate participatory events, which support the social interactions of the organisation and the extended community. This thesis examines how scale and materiality can nurture the individual and group experience, and how this might be tested at the scale of the city, street, organisation, event, parcel, and building

Relative Roles of Climate Sensitivity and Forcing in Defining the Ocean Circulation Response to Climate Change

Abstract in HTML and technical report in PDF available on the Massachusetts Institute of Technology Joint Program on the Science and Policy of Global Change website (http://mit.edu/globalchange/www/).The response of the ocean’s meridional overturning circulation (MOC) to increased greenhouse gas forcing is examined using a coupled model of intermediate complexity, including a dynamic 3D ocean subcomponent. Parameters are the increase in CO2 forcing (with stabilization after a specified time interval) and the model’s climate sensitivity. In this model, the cessation of deep sinking in the north “Atlantic” (hereinafter, a “collapse”), as indicated by changes in the MOC, behaves like a simple bifurcation. The final surface air temperature (SAT) change, which is closely predicted by the product of the radiative forcing and the climate sensitivity, determines whether a collapse occurs. The initial transient response in SAT is largely a function of the forcing increase, with higher sensitivity runs exhibiting delayed behavior; accordingly, high CO2-low sensitivity scenarios can be assessed as a recovering or collapsing circulation shortly after stabilization, whereas low CO2-high sensitivity scenarios require several hundred additional years to make such a determination. We also systemically examine how the rate of forcing, for a given CO2 stabilization, affects the ocean response. In contrast with previous studies based on results using simpler ocean models, we find that except for a narrow range of marginally stable to marginally unstable scenarios, the forcing rate has little impact on whether the run collapses or recovers. In this narrow range, however, forcing increases on a time scale of slow ocean advective processes results in weaker declines in overturning strength and can permit a run to recover that would otherwise collapse.This research was supported in part by the Methods and Models for Integrated Assessments Program of the National Science Foundation, Grant ATM-9909139, by the Office of Science (BER), U.S. Department of Energy, Grant No. DE-FG02-93ER61677, and by the MIT Joint Program on the Science and Policy of Global Change (JPSPGC)

Reassortment and Interspecies Transmission of North American H6N2 Influenza Viruses

AbstractH6N2 influenza viruses were isolated from California chickens in 2000 and 2001. Here we report the characterization of these H6N2 viruses, one of the few descriptions of non-H5, non-H7 subtype influenza viruses in this host. The H6N2 viruses were nonpathogenic in experimentally infected chickens and could be divided into three genotypes. All three genotypes of virus had similar surface glycoproteins and all contained an 18 amino acid deletion in the neuraminidase, a characteristic of other chicken influenza viruses. Differences were apparent, however, in the complement of replicative protein genes between the genotypes. The presence of multiple H6N2 genotypes suggests that independent transmission and/or reassortment events may have taken place between aquatic bird and chicken influenza viruses

Role of social environment and social clustering in spread of opinions in co-evolving networks

Taking a pragmatic approach to the processes involved in the phenomena of collective opinion formation, we investigate two specific modifications to the co-evolving network voter model of opinion formation, studied by Holme and Newman [1]. First, we replace the rewiring probability parameter by a distribution of probability of accepting or rejecting opinions between individuals, accounting for the asymmetric influences in relationships among individuals in a social group. Second, we modify the rewiring step by a path-length-based preference for rewiring that reinforces local clustering. We have investigated the influences of these modifications on the outcomes of the simulations of this model. We found that varying the shape of the distribution of probability of accepting or rejecting opinions can lead to the emergence of two qualitatively distinct final states, one having several isolated connected components each in internal consensus leading to the existence of diverse set of opinions and the other having one single dominant connected component with each node within it having the same opinion. Furthermore, and more importantly, we found that the initial clustering in network can also induce similar transitions. Our investigation also brings forward that these transitions are governed by a weak and complex dependence on system size. We found that the networks in the final states of the model have rich structural properties including the small world property for some parameter regimes. [1] P. Holme and M. Newman, Phys. Rev. E 74, 056108 (2006)

In Vitro Antitumour Activity of Some Triorganophosphinegold(I) Thionucleobases

A series of phosphinegold(I) thionucleobase analogues, [R3PAu(SRx)] (R = Et, Ph or chexyl; HSR1 = 2-mercaptobenzoic acid, HSR2 = 2-thiouracil, HSR3 = 6-mercaptopurine and HSR4 = 6-thioguanine) have been examined for their in vitro cytotoxicity in L1210 murine leukemia cells in culture. The range of ID50 values (continuous 48 h exposure) for the complexes is 0.041 - 0.131 μM. The complexes with SR3 and SR3 are generally the most active; however, there is no clear trend associated with the phosphine ligands

Variability in Microbial Community Composition and Function Between Different Niches Within a Coral Reef

To explore how microbial community composition and function varies within a coral reef ecosystem, we performed metagenomic sequencing of seawater from four niches across Heron Island Reef, within the Great Barrier Reef. Metagenomes were sequenced from seawater samples associated with (1) the surface of the coral species Acropora palifera, (2) the surface of the coral species Acropora aspera, (3) the sandy substrate within the reef lagoon and (4) open water, outside of the reef crest. Microbial composition and metabolic function differed substantially between the four niches. The taxonomic profile showed a clear shift from an oligotroph-dominated community (e.g. SAR11, Prochlorococcus, Synechococcus) in the open water and sandy substrate niches, to a community characterised by an increased frequency of copiotrophic bacteria (e.g. Vibrio, Pseudoalteromonas, Alteromonas) in the coral seawater niches. The metabolic potential of the four microbial assemblages also displayed significant differences, with the open water and sandy substrate niches dominated by genes associated with core house-keeping processes such as amino acid, carbohydrate and protein metabolism as well as DNA and RNA synthesis and metabolism. In contrast, the coral surface seawater metagenomes had an enhanced frequency of genes associated with dynamic processes including motility and chemotaxis, regulation and cell signalling. These findings demonstrate that the composition and function of microbial communities are highly variable between niches within coral reef ecosystems and that coral reefs host heterogeneous microbial communities that are likely shaped by habitat structure, presence of animal hosts and local biogeochemical conditions.Human Frontier Science Program (Strasbourg, France) (Grant RGY0089/2011

Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs.

Inherited retinal dystrophies are an important cause of blindness, for which currently there are no effective treatments. In order to study this heterogeneous group of diseases, adequate disease models are required in order to better understand pathology and to test potential therapies. Induced pluripotent stem cells offer a new way to recapitulate patient specific diseases in vitro, providing an almost limitless amount of material to study. We used fibroblast-derived induced pluripotent stem cells to generate retinal pigment epithelium (RPE) from an individual suffering from retinitis pigmentosa associated with biallelic variants in MERTK. MERTK has an essential role in phagocytosis, one of the major functions of the RPE. The MERTK deficiency in this individual results from a nonsense variant and so the MERTK-RPE cells were subsequently treated with two translational readthrough inducing drugs (G418 & PTC124) to investigate potential restoration of expression of the affected gene and production of a full-length protein. The data show that PTC124 was able to reinstate phagocytosis of labeled photoreceptor outer segments at a reduced, but significant level. These findings represent a confirmation of the usefulness of iPSC derived disease specific models in investigating the pathogenesis and screening potential treatments for these rare blinding disorders

Three-dimensional model study of the Arctic ozone loss in 2002/2003 and comparison with 1999/2000 and 2003/2004

We have used the SLIMCAT 3-D off-line chemical transport model (CTM) to quantify the Arctic chemical ozone loss in the year 2002/2003 and compare it with similar calculations for the winters 1999/2000 and 2003/2004. Recent changes to the CTM have improved the model's ability to reproduce polar chemical and dynamical processes. The updated CTM uses σ-θ as a vertical coordinate which allows it to extend down to the surface. The CTM has a detailed stratospheric chemistry scheme and now includes a simple NAT-based denitrification scheme in the stratosphere. In the model runs presented here the model was forced by ECMWF ERA40 and operational analyses. The model used 24 levels extending from the surface to ~55km and a horizontal resolution of either 7.5° x 7.5° or 2.8° x 2.8°. Two different radiation schemes, MIDRAD and the CCM scheme, were used to diagnose the vertical motion in the stratosphere. Based on tracer observations from balloons and aircraft, the more sophisticated CCM scheme gives a better representation of the vertical transport in this model which includes the troposphere. The higher resolution model generally produces larger chemical O3 depletion, which agrees better with observations. The CTM results show that very early chemical ozone loss occurred in December 2002 due to extremely low temperatures and early chlorine activation in the lower stratosphere. Thus, chemical loss in this winter started earlier than in the other two winters studied here. In 2002/2003 the local polar ozone loss in the lower stratosphere was ~40% before the stratospheric final warming. Larger ozone loss occurred in the cold year 1999/2000 which had a persistently cold and stable vortex during most of the winter. For this winter the current model, at a resolution of 2.8° x 2.8°, can reproduce the observed loss of over 70% locally. In the warm and more disturbed winter 2003/2004 the chemical O3 loss was generally much smaller, except above 620K where large losses occurred due to a period of very low minimum temperatures at these altitudes

The Gsp-1 genes encode the 1 wheat arabinogalactan peptide

Western blotting, ELISA and 1H-NMR spectroscopy showed that RNAi down-regulation of the wheat Gsp-1 gene resulted in reduced contents of both arabinogalactan peptide (AGP) and grain softness protein (GSP-1) in mature wheat grains confirming that these components are encoded by the same gene. A small increase in grain hardness and decrease in the viscosity of aqueous extracts of the transgenic lines also indicated small effects on functional properties. Immunolocalisation using a novel wheat AGP monoclonal antibody in conjunction with confocal microscopy showed that the major form of AGP which was eliminated in knockout lines is located within the cell, probably in the vacuole, and not in the plasma membrane or cell wall. However, clear localisation of the AGP epitope to the plasma membrane was observed in both control and transgenic lines and probably resulted from the presence of one or more separate forms of arabinogalactan protein. The existence of such additional form(s) was also indicated by 1H-NMR spectroscopy which showed that the ratio of arabinose to galactose differed between the control and transgenic line