1,279 research outputs found

    Adapting the Customer Satisfaction Index to the Lodging Industry: Foreign Customers\u27 Evaluations

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    As a standard form of measuring customer satisfaction, the Customer Satisfaction Index (CSI) has been utilized in many countries. By using the Korean Customer Satisfaction Index (KCSI) methodology, this study attempted to investigate foreign customers’ evaluations of luxury hotels in Seoul, South Korea. In doing so, some efforts were made to overcome the methodological problems associated with the KCSI for the lodging industry. Data for this study were collected through a mall intercept survey using a self-administered questionnaire. Precisely 783 responses, collected solely from foreign guests who had stayed at a luxury hotel in Seoul, were included in the study

    Statistical Models for Hot Electron Degradation in Nano-Scaled MOSFET Devices

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    In a MOS structure, the generation of hot carrier interface states is a critical feature of the item\u27s reliability. On the nano-scale, there are problems with degradation in transconductance, shift in threshold voltage, and decrease in drain current capability. Quantum mechanics has been used to relate this decrease to degradation, and device failure. Although the lifetime, and degradation of a device are typically used to characterize its reliability, in this paper we model the distribution of hot-electron activation energies, which has appeal because it exhibits a two-point discrete mixture of logistic distributions. The logistic mixture presents computational problems that are addressed in simulation

    Attitudes and Perceptions toward Affirmative Action Programs: An Application of Institutional Theory

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    Due to the continuing controversy surrounding the use of affirmative action programs in organizational hiring and promotion practices, we conducted a study to model individuals’ attitudes, beliefs, and perceptions toward affirmative action (AA) programs considering social influences and an institutional theory framework. To conduct the study we surveyed 413 managers and supervisors from three large hotel companies in the pacific western and southeastern United States. Results indicate that institutional and social forces positively influenced participants’ general perceptions of AA in the workplace. Influences from social interaction exhibited a negative relationship upon attitudes toward the experience and practice of AA, specifically related to AA hires and organizational issues stemming from AA rather than the legislation itself

    Lysine-Cysteine-Lysine (KCK) tag changes ParB action in vitro but not in vivo

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    Due to the enhanced labeling capability of maleimide-based fluorescent probes in in vitro experiments, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. Here we show that, although no noticeable changes were detected from in vivo fluorescence imaging and chromatin immunoprecipitation (ChIP) assays, the KCK-tag substantially altered DNA compaction rates by Bacillus subtilis ParB protein in in vitro single-molecule DNA flow-stretching experiments. Furthermore, our measurements and statistical analyses demonstrate that the KCK-tags also altered the ParB protein’s response to nucleotide (cytidine triphosphate CTP or its nonhydrolyzable analog CTPγS) binding and the presence of the specific DNA binding sequence (parS). Remarkably, the appended KCK-tags are capable of even reversing the trends of DNA compaction rates upon different experimental conditions. DNA flow-stretching experiments for both fluorescently-labeled ParB proteins and ParB proteins with an N-terminal glutamic acid-cysteine-glutamic acid (ECE) tag support the notion that electrostatic interactions between charges on the tags and the DNA backbone are an underlying cause of the protein’s property changes. While it is typically assumed that the short KCK-tag minimally perturbs protein function, our results demonstrate that this assumption must be carefully tested when using tags for protein labeling

    Syntheses of 3-[(Alkylamino)methylene]-6-methyl-1Hpyridine-2,4-diones, Fluorescence Probes 3-Substituted 7-Methyl-6H-pyrano[3,2-c]pyridine-2,5-diones, and Tetrahydro-6H-2,10-dioxa-9-azaanthracen-1-ones

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    Various condensation and ring-closing reactions were used for the syntheses of 3-[(alkylamino)methylene]-6-methylpyridine-2,4(1H,3H)-diones, bicyclic pyridinones, and tricyclic morpholinopyrones. For instance, 3-[(dialkylamino)methylene]-6-methylpyridine-2,4(1H,3H)-diones were synthesized from the condensation of dialkylamines and 3-formyl-4-hydroxy-6-methylpyridin-2(1H)-one. 3-Formyl-4-hydroxy-6-methylpyridin-2(1H)-one, derived from 3-formyl-4-hydroxy-6-methylpyridin-2(1H)-one, was used to construct a number of bicyclic pyridinones via a one-pot Knoevenagal and intramolecular lactonization reaction. Tricyclic morpholinopyrones were assembled from a dialkylation reaction involving a dinucleophile, 3-amino-4-hydroxy-6-methyl-2H-pyran-2-one, and a dielectrophile, trans-3,6-dibromocyclohexene. Depending on the reaction conditions, isomers of the tricyclic molecules can be selectively produced, and their chemical structures were unequivocally determined using single-crystal X-ray analyses and 2D COSY spectroscopy. The fluorescently active bicyclic pyridinone compounds show longer absorption (368–430 nm; maximum) and emission wavelengths (450–467 nm) than those of 7-amino-4-methylcoumarin (AMC; λ[subscript abs,max] = 350 nm; λ[subscript em] = 430 nm) suggesting these molecules, such as 3-(2-aminoacetyl)-7-methyl-2H-pyrano[3,2-c]pyridine-2,5(6H)-dione, can be employed as fluorescence activity based probes for tracing biological pathways

    Impact of Redshift Information on Cosmological Applications with Next-Generation Radio Surveys

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    In this paper, we explore how the forthcoming generation of large-scale radio continuum surveys, with the inclusion of some degree of redshift information, can constrain cosmological parameters. By cross-matching these radio surveys with shallow optical to near-infrared surveys, we can essentially separate the source distribution into a low- and a high-redshift sample, thus providing a constraint on the evolution of cosmological parameters such as those related to dark energy. We examine two radio surveys, the Evolutionary Map of the Universe (EMU) and the Westerbork Observations of the Deep APERTIF Northern sky (WODAN). A crucial advantage is their combined potential to provide a deep, full-sky survey. The surveys used for the cross-identifications are SkyMapper and SDSS, for the southern and northern skies, respectively. We concentrate on the galaxy clustering angular power spectrum as our benchmark observable, and find that the possibility of including such low redshift information yields major improvements in the determination of cosmological parameters. With this approach, and provided a good knowledge of the galaxy bias evolution, we are able to put strict constraints on the dark energy parameters, i.e. w_0=-0.9+/-0.041 and w_a=-0.24+/-0.13, with type Ia supernovae and CMB priors (with a one-parameter bias in this case); this corresponds to a Figure of Merit (FoM) > 600, which is twice better than what is obtained by using only the cross-identified sources and greater than four time better than the case without any redshift information at all.Comment: 12 pages, 6 figures, 6 tables; accepted for publication in MNRA

    Percutaneous Transhepatic Cholangioscopic Intervention in the Management of Complete Membranous Occlusion of Bilioenteric Anastomosis: Report of Two Cases

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    Postoperative biliary stricture is a relatively rare but serious complication of biliary surgery. Although Rouxen-Y hepaticojejunostomy or choledochojejunostomy are well-established and fundamental therapeutic approaches, their postoperative morbidity and mortality rates have been reported to be up to 33% and 13%, respectively. Recent studies suggest that percutaneous transhepatic intervention is an effective and less invasive therapeutic modality compared with traditional surgical treatment. Compared with fluoroscopic intervention, percutaneous with cholangioscopy may be more useful in biliary strictures, as it can provide visual information regarding the stricture site. We recently experienced two cases complete membranous occlusion of the bilioenteric anastomosis and successfully treated both patients using percutaneous transhepatic cholangioscopy

    Bayesian Cluster Finder: Clusters in the CFHTLS Archive Research Survey

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    The detection of galaxy clusters in present and future surveys enables measuring mass-to-light ratios, clustering properties, galaxy cluster abundances and therefore, constraining cosmological parameters. We present a new technique for detecting galaxy clusters, which is based on the Matched Filter Algorithm from a Bayesian point of view. The method is able to determine the position, redshift and richness of the cluster through the maximization of a filter depending on galaxy luminosity, density and photometric redshift combined with a galaxy cluster prior that accounts for color-magnitude relations and BCG-redshift relation. We tested the algorithm through realistic mock galaxy catalogs, revealing that the detections are 100% complete and 80% pure for clusters up to z 20 (Abell Richness ∌\sim0, M∌4×1014M⊙\sim4\times10^{14} M_{\odot}). The completeness and purity remains approximately the same if we do not include the prior information, implying that this method is able to detect galaxy cluster with and without a well defined red sequence. We applied the algorithm to the CFHTLS Archive Research Survey (CARS) data, recovering similar detections as previously published using the same or deeper data plus additional clusters which appear to be real.Comment: Accepted for publication in MNRAS; 17 pages, 38 figure
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