15 research outputs found
Crystal Structure and Computational Study on Methyl-3-Aminothiophene-2-Carboxylate
Methyl-3-aminothiophene-2-carboxylate (matc) is a key intermediate in organic synthesis, medicine, dyes, and pesticides. Single crystal X-ray diffraction analysis reveals that matc crystallizes in the monoclinic crystal system P21/c space group. Three matc molecules in the symmetric unit are crystallographically different and further linked through the N–H⋯O and N–H⋯N hydrogen bond interactions along with weak C–H⋯S and C–H⋯Cg interactions, which is verified by the three-dimensional Hirshfeld surface, two-dimensional fingerprint plot, and reduced density gradient (RDG) analysis. The interaction energies within crystal packing are visualized through dispersion, electrostatic, and total energies using three-dimensional energy-framework analyses. The dispersion energy dominates in crystal packing. To better understand the properties of matc, electrostatic potential (ESP) and frontier molecular orbitals (FMO) were also calculated and discussed. Experimental and calculation results suggested that amino and carboxyl groups can participate in various inter- and intra-interactions
Highly efficient isolation and 3D printing of fibroblasts for cultured meat production
Fibroblasts are important components of animal tissues such as muscle and skin, as they are the major producers of various matrix proteins. Matrix proteins such as collagen play an important role in meat products by providing unique nutrition, texture, and flavor. Cultured meat is an innovative meat alternative produced by culturing animal cells, but currently, relatively few studies have been conducted using fibroblasts as seed cells for cultured meat manufacturing. In this work, we first developed an innovative digestion-friction method for isolating fibroblasts from porcine skin efficiently and cost-effectively. After optimizing the enzymatic digestion and physical friction conditions, 2.39 ± 0.28 × 105 fibroblasts were obtained from 1 cm2 of porcine skin tissue, which was about 9 times higher than the conventional tissue explant method. In addition, we identified an edible bio-ink composed of gelatin and chitosan that has good printing properties and supports fibroblast adhesion and growth. Furthermore, we fabricated fibroblast-based cultured meat by 3D printing with an initial cell density of 1.0 × 107 mL−1 and evaluated its texture and nutritional properties. This work provides valuable insights and references for introducing fibroblasts into the production of cultured meat that is more comparable to structured animal meat
Investigating the antiferroelectric-ferroelectric phase switching time in lanthanum-modified lead zirconate titanate transparent ceramics by using the electrically induced light scattering effect
Solvent-Type Passivation Strategy Controls Solid-State Self-Quenching-Resistant Behavior in Sulfur Dots
Treatment of sulfur dots with polyethylene glycol (PEG)
has been
an efficient way to achieve a high luminescence quantum yield, and
such a PEG-related quantum dot (QD)-synthesis strategy has been well
documented. However, the polymeric insulating capping layer acting
as the “thick shell” will significantly slow down the
electron-transfer efficiency and severely hamper its practical application
in an optoelectric field. Especially, the employment of synthetic
polymers with long alkyl chains or large molecular weights may lead
to structural complexity or even unexpected changes of physical characteristics
for QDs. Therefore, in sulfur dot preparation, it is a breakthrough
to use short-chain molecular species to replace PEG for better control
and reproducibility. In this article, a solvent-type passivation (STP)
strategy has been reported, and no PEG or any other capping agent
is required. The main role of the solvent, ethanol, is to directly
react with NaOH, and the generated sodium ethoxide passivates the
surface defects. The afforded STP-enhanced emission sulfur dots (STPEE-SDs)
possess not only the self-quenching-resistant feature in the solid
state but also the extension of fluorescence band toward the wavelength
as long as 645 nm. The realization of sulfur dot emission in the deep-red
region with a decent yield (8.7%) has never been reported. Moreover,
a super large Stokes shift (300 nm, λex = 345 nm,
λem = 645 nm) and a much longer decay lifetime (109
μs) have been found, and such values can facilitate to suppress
the negative influence from background signals. Density functional
theory demonstrates that the surface passivation via sodium ethoxide
is dynamically favorable, and the spectroscopic insights into emission
behavior could be derived from the passivation effect of the sulfur
vacancy as well as the charge-transfer process dominated by the highly
electronegative ethoxide layer