Low Reproductive Success of Mallards in a Grassland-Dominated Landscape in The Sandhills of Nebraska

The Sandhills of Nebraska comprise approximately 5,000,000 ha of native grassland interspersed with numerous groundwater-fed wetlands. A substantial population of the mallard (Anas platyrhynchos) nests in this region. Previous investigations of nest survival probability of ducks in the Sandhills have estimated surprisingly low rates of nest survival for a grassland-dominated landscape. These investigations were conducted on public lands and most nest searching took place near wetlands where activity of nest predators might be highest. We predicted that mallards would nest at varying distances from wetlands and that survival probability of a representative sample of duck nests would increase with distance from wetlands. We decoy-trapped and radio-marked 71 female mallards, 32 during the 2005 nesting season and 39 during the 2006 nesting season, and monitored their individual choice of nest habitats, their survival during the nesting season, and survival of their nests. Mallards nested in various habitats, both near and far from wetlands. Nest survival probability (Ö = 0.03, SE = 0.02) was low relative to other studies regardless of distance to wetlands. Survival of females during the nesting season (Ö = 0.84, SE = 0.08), however, was high relative to other studies. This pattern could have resulted from the combination of a diverse community of nest predators, few predators of nesting females, and a population of largely second year females that put little effort into nesting

Low Reproductive Success of Mallards in a Grassland-Dominated Landscape in The Sandhills of Nebraska

The Sandhills of Nebraska comprise approximately 5,000,000 ha of native grassland interspersed with numerous groundwater-fed wetlands. A substantial population of the mallard (Anas platyrhynchos) nests in this region. Previous investigations of nest survival probability of ducks in the Sandhills have estimated surprisingly low rates of nest survival for a grassland-dominated landscape. These investigations were conducted on public lands and most nest searching took place near wetlands where activity of nest predators might be highest. We predicted that mallards would nest at varying distances from wetlands and that survival probability of a representative sample of duck nests would increase with distance from wetlands. We decoy-trapped and radio-marked 71 female mallards, 32 during the 2005 nesting season and 39 during the 2006 nesting season, and monitored their individual choice of nest habitats, their survival during the nesting season, and survival of their nests. Mallards nested in various habitats, both near and far from wetlands. Nest survival probability (Ö = 0.03, SE = 0.02) was low relative to other studies regardless of distance to wetlands. Survival of females during the nesting season (Ö = 0.84, SE = 0.08), however, was high relative to other studies. This pattern could have resulted from the combination of a diverse community of nest predators, few predators of nesting females, and a population of largely second year females that put little effort into nesting

A synthesis of knowledge about motives for participation in perpetual conservation easements

Perpetual conservation easements are a popular method in some countries for addressing conservation goals. Landowner participation plays a key role in the development of these agreements. Despite the importance of involvement by landowners, no recent efforts have been made to synthesize information about the motivations for participation in perpetual easement programs. As a result, the literature lacks a framework to guide future case studies that would facilitate comparisons and generalizations. To this end, we reviewed 43 studies that investigated individual motivations to participate in perpetual conservation easements, and categorized motivations using Ostrom's social–ecological framework. We identified a strong tendency among studies to focus only on local-scale processes involving landowners, with little consideration of broader-scale influences. We also highlight several cross-study trends and gaps in the literature where future research would prove valuable

Is the observed NAO variability during the instrumental record unusual?

Observed multidecadal variability (30 yr running means, trends, and moving standard deviations) of the North Atlantic Oscillation (NAO) during the instrumental record is compared to that simulated by two different coupled general circulation models in extended-range control experiments. Simulated NAO exhibits strong low frequency fluctuations, even on multi-centennial time scale. Observed multi-decadal NAO variations agree well with the model variability. Trend probability distribution functions, observed and simulated, were not found to be different with statistical significance. Thus, multi-decadal NAO changes similar to those observed during the instrumental record, including the recent increase in 1965–1995, may be internally generated within the coupled atmosphere-ocean system without considering external forcing

Dynamics of social class contempt in contemporary British television comedy

This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2010 Taylor & Francis.British television comedy has often ridiculed the complexities and characteristics of social class structures and identities. In recent years, poor white socially marginalised groups, now popularly referred to as “chavs”, have become a prevalent comedy target. One of the most popular and controversial television “comedy chavs” is Little Britain's fictional teenage single mother, Vicky Pollard. This article examines the representation of Vicky Pollard in light of contemporary widespread abuse of the white working class. Highlighting the polysemic and ambivalent nature of Vicky Pollard's representation, the article argues that whilst Little Britain's characterisation of Vicky Pollard largely contributes to contemporary widespread demonisation of the working class, there are moments within Little Britain when a more sympathetic tone towards the poor working class may be read, and where chav identities are used to ridicule the pretensions, superficiality, and falsity of middle-class identities. The article concludes that television comedy has been, and continues to be, a significant vehicle through which serious concerns, anxieties, and questions about social class and class identities are discursively constructed and contested

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

Finding needles in haystacks:Linking scientific names, reference specimens and molecular data for Fungi

DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data, tied to both correct taxonomic names and clearly annotated specimen data, has never been greater. Furthermore, the growing number of molecular ecology and microbiome projects using high-throughput sequencing require fast and effective methods for en masse species assignments. In this article, we focus on selecting and re-annotating a set of marker reference sequences that represent each currently accepted order of Fungi. The particular focus is on sequences from the internal transcribed spacer region in the nuclear ribosomal cistron, derived from type specimens and/or ex-type cultures. Reannotated and verified sequences were deposited in a curated public database at the National Center for Biotechnology Information (NCBI), namely the RefSeq Targeted Loci (RTL) database, and will be visible during routine sequence similarity searches with NR_prefixed accession numbers. A set of standards and protocols is proposed to improve the data quality of new sequences, and we suggest how type and other reference sequences can be used to improve identification of Fungi.The Intramural Research Programs of the National Center for Biotechnology Information, National Library of Medicine and the National Human Genome Research Institute, both at the National Institutes of Health.http://www.ncbi.nlm.nih.gov/bioproject/PRJNA177353am201

Cross-oncopanel study reveals high sensitivity and accuracy with overall analytical performance depending on genomic regions

BackgroundTargeted sequencing using oncopanels requires comprehensive assessments of accuracy and detection sensitivity to ensure analytical validity. By employing reference materials characterized by the U.S. Food and Drug Administration-led SEquence Quality Control project phase2 (SEQC2) effort, we perform a cross-platform multi-lab evaluation of eight Pan-Cancer panels to assess best practices for oncopanel sequencing.ResultsAll panels demonstrate high sensitivity across targeted high-confidence coding regions and variant types for the variants previously verified to have variant allele frequency (VAF) in the 5-20% range. Sensitivity is reduced by utilizing VAF thresholds due to inherent variability in VAF measurements. Enforcing a VAF threshold for reporting has a positive impact on reducing false positive calls. Importantly, the false positive rate is found to be significantly higher outside the high-confidence coding regions, resulting in lower reproducibility. Thus, region restriction and VAF thresholds lead to low relative technical variability in estimating promising biomarkers and tumor mutational burden.ConclusionThis comprehensive study provides actionable guidelines for oncopanel sequencing and clear evidence that supports a simplified approach to assess the analytical performance of oncopanels. It will facilitate the rapid implementation, validation, and quality control of oncopanels in clinical use.Peer reviewe

Decline in subarachnoid haemorrhage volumes associated with the first wave of the COVID-19 pandemic

BACKGROUND: During the COVID-19 pandemic, decreased volumes of stroke admissions and mechanical thrombectomy were reported. The study\u27s objective was to examine whether subarachnoid haemorrhage (SAH) hospitalisations and ruptured aneurysm coiling interventions demonstrated similar declines. METHODS: We conducted a cross-sectional, retrospective, observational study across 6 continents, 37 countries and 140 comprehensive stroke centres. Patients with the diagnosis of SAH, aneurysmal SAH, ruptured aneurysm coiling interventions and COVID-19 were identified by prospective aneurysm databases or by International Classification of Diseases, 10th Revision, codes. The 3-month cumulative volume, monthly volumes for SAH hospitalisations and ruptured aneurysm coiling procedures were compared for the period before (1 year and immediately before) and during the pandemic, defined as 1 March-31 May 2020. The prior 1-year control period (1 March-31 May 2019) was obtained to account for seasonal variation. FINDINGS: There was a significant decline in SAH hospitalisations, with 2044 admissions in the 3 months immediately before and 1585 admissions during the pandemic, representing a relative decline of 22.5% (95% CI -24.3% to -20.7%, p\u3c0.0001). Embolisation of ruptured aneurysms declined with 1170-1035 procedures, respectively, representing an 11.5% (95%CI -13.5% to -9.8%, p=0.002) relative drop. Subgroup analysis was noted for aneurysmal SAH hospitalisation decline from 834 to 626 hospitalisations, a 24.9% relative decline (95% CI -28.0% to -22.1%, p\u3c0.0001). A relative increase in ruptured aneurysm coiling was noted in low coiling volume hospitals of 41.1% (95% CI 32.3% to 50.6%, p=0.008) despite a decrease in SAH admissions in this tertile. INTERPRETATION: There was a relative decrease in the volume of SAH hospitalisations, aneurysmal SAH hospitalisations and ruptured aneurysm embolisations during the COVID-19 pandemic. These findings in SAH are consistent with a decrease in other emergencies, such as stroke and myocardial infarction