Effect of high fructose administration on histopathology of kidney, heart and aorta of rats

Objective: This study was conducted to assess the hazardous effects of high fructose administration on kidney, heart and aorta in rats. Materials and methods: Twenty adult healthy male albino rats weighing about 200-220 gm each were used in this study. The rats were divided into 2 duplicate groups; control group and fructose group. Fructose was administered to rats in fresh drinking water daily for 8 weeks (the whole experimental period). Serum urea, creatinine and sodium concentration were determined by using ready-made kits. Spectrophotometric and colorimetric methods were also used for the detection of other serum components. Histopathological examination of the tissues was done by staining with H&E, PAS and Masson trichrome stains. Results: Nephropathy was achieved in fructose group after one month as indicated by biochemical assay. Pathological observation showed that high fructose administration decreased size of cardio-myocytes, increased cardiac interstitial fibrosis score and aortic wall thickness. In kidneys, high fructose administration decreased glomerular tuft area and corpuscular area, increased percentage in the rats affected with interstitial renal fibrosis score 1 and percentage of rats had glomerular sclerosis score 2. Conclusion: High fructose in diet should be avoided because it can damage kidney, heart and aorta in rats. [J Adv Vet Anim Res 2017; 4(1.000): 71-79

Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells

Objective: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells. Methods: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy. Results: The IC50 values of A. vera and C. comosum extracts were (10.45 ± 0.31) and (9.60 ± 0.01) µg/mL respectively. The extracts separately increased cytotoxicity against HepG2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions. Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis

Lipid Profile and Obese Related Genes of Rats, Potential Therapeutic Effects of Peganum Harmala /Zinc Oxide Nanoparticles

The present study investigated the effects of methanolic extract of harmala nanoparticle (H/ZnONP) on the changes in serum lipid profiles, serum leptin levels and liver and kidney PPAR gene expression in high caloric diet (HCD) induced obese rats. After induction of obesity with HCD for one month to half numbers of rats. Non-obese group had three subgroups; G1: Control fed basal diets, G2: basal diets plus H/ZnONPs (200 mg/kg/day) and G3: basal diets plus H/ZnONPs (400 mg/kg/day). However, obese group was divided into three subgroups G4: HFD, G5: HFD+H/ZnONPs (200 mg/kg/day) and G6: HFD+ H/ZnONPs (400 mg/kg/day). After 4 weeks of treatment blood and tissue samples were collected and subjected to laboratory assay of lipid profile and leptin level in addition to expression of PPAR gene in liver and kidney. We also investigated the possible protective effect of H/ZnONP 200 and 400 mg/kg BW on liver and kidney histology. The obtained results demonstrated that H/ZnONP treatment to obese rats significantly decreased serum cholesterols, triglyceride, and LDL level while relative expression of PPAR gene in liver and kidney was significantly increased. Based on the obtained results we can concluded that H/ZnONP supplementation has a potent anti-obesity effect in rats by improving lipid profile, enhance expression of PPAR gene in liver and kidney and improve liver and kidney histology particularly the high dose

Lipid Profile and Obese Related Genes of Rats, Potential Therapeutic Effects of Peganum Harmala /Zinc Oxide Nanoparticles

The present study investigated the effects of methanolic extract of harmala nanoparticle (H/ZnONP) on the changes in serum lipid profiles, serum leptin levels and liver and kidney PPAR gene expression in high caloric diet (HCD) induced obese rats. After induction of obesity with HCD for one month to half numbers of rats. Non-obese group had three subgroups; G1: Control fed basal diets, G2: basal diets plus H/ZnONPs (200 mg/kg/day) and G3: basal diets plus H/ZnONPs (400 mg/kg/day). However, obese group was divided into three subgroups G4: HFD, G5: HFD+H/ZnONPs (200 mg/kg/day) and G6: HFD+ H/ZnONPs (400 mg/kg/day). After 4 weeks of treatment blood and tissue samples were collected and subjected to laboratory assay of lipid profile and leptin level in addition to expression of PPAR gene in liver and kidney. We also investigated the possible protective effect of H/ZnONP 200 and 400 mg/kg BW on liver and kidney histology. The obtained results demonstrated that H/ZnONP treatment to obese rats significantly decreased serum cholesterols, triglyceride, and LDL level while relative expression of PPAR gene in liver and kidney was significantly increased. Based on the obtained results we can concluded that H/ZnONP supplementation has a potent anti-obesity effect in rats by improving lipid profile, enhance expression of PPAR gene in liver and kidney and improve liver and kidney histology particularly the high dose