104 research outputs found
Ultrafast control of donor-bound electron spins with single detuned optical pulses
The ability to control spins in semiconductors is important in a variety of
fields including spintronics and quantum information processing. Due to the
potentially fast dephasing times of spins in the solid state [1-3], spin
control operating on the picosecond or faster timescale may be necessary. Such
speeds, which are not possible to attain with standard electron spin resonance
(ESR) techniques based on microwave sources, can be attained with broadband
optical pulses. One promising ultrafast technique utilizes single broadband
pulses detuned from resonance in a three-level Lambda system [4]. This
attractive technique is robust against optical pulse imperfections and does not
require a fixed optical reference phase. Here we demonstrate the principle of
coherent manipulation of spins theoretically and experimentally. Using this
technique, donor-bound electron spin rotations with single-pulse areas
exceeding pi/4 and two-pulses areas exceeding pi/2 are demonstrated. We believe
the maximum pulse areas attained do not reflect a fundamental limit of the
technique and larger pulse areas could be achieved in other material systems.
This technique has applications from basic solid-state ESR spectroscopy to
arbitrary single-qubit rotations [4, 5] and bang-bang control[6] for quantum
computation.Comment: 15 pages, 4 figures, submitted 12/2008. Since the submission of this
work we have become aware of related work: J. Berezovsky, M. H. Mikkelsen, N.
G. Stoltz, L. A. Coldren, and D. D. Awschalom, Science 320: 349-352 (2008
Image Feature Extraction Acceleration
Image feature extraction is instrumental for most of the best-performing algorithms in computer vision. However, it is also expensive in terms of computational and memory resources for embedded systems due to the need of dealing with individual pixels at the earliest processing levels. In this regard, conventional system architectures do not take advantage of potential exploitation of parallelism and distributed memory from the very beginning of the processing chain. Raw pixel values provided by the front-end image sensor are squeezed into a high-speed interface with the rest of system components. Only then, after deserializing this massive dataflow, parallelism, if any, is exploited. This chapter introduces a rather different approach from an architectural point of view. We present two Application-Specific Integrated Circuits (ASICs) where the 2-D array of photo-sensitive devices featured by regular imagers is combined with distributed memory supporting concurrent processing. Custom circuitry is added per pixel in order to accelerate image feature extraction right at the focal plane. Specifically, the proposed sensing-processing chips aim at the acceleration of two flagships algorithms within the computer vision community: the Viola-Jones face detection algorithm and the Scale Invariant Feature Transform (SIFT). Experimental results prove the feasibility and benefits of this architectural solution.Ministerio de Economía y Competitividad TEC2012-38921-C02, IPT-2011- 1625-430000, IPC-20111009Junta de Andalucía TIC 2338-2013Xunta de Galicia EM2013/038Office of NavalResearch (USA) N00014141035
Multiple Deeply Divergent Denisovan Ancestries in Papuans
Genome sequences are known for two archaic
hominins—Neanderthals and Denisovans—which
interbred with anatomically modern humans as
they dispersed out of Africa. We identified high-confidence
archaic haplotypes in 161 new genomes
spanning 14 island groups in Island Southeast
Asia and New Guinea and found large stretches of
DNA that are inconsistent with a single introgressing
Denisovan origin. Instead, modern Papuans carry
hundreds of gene variants from two deeply divergent
Denisovan lineages that separated over 350
thousand years ago. Spatial and temporal structure
among these lineages suggest that introgression
from one of these Denisovan groups predominantly
took place east of the Wallace line and continued
until near the end of the Pleistocene. A third Denisovan
lineage occurs in modern East Asians. This
regional mosaic suggests considerable complexity
in archaic contact, with modern humans interbreeding
with multiple Denisovan groups that were
geographically isolated from each other over deep
evolutionary time
Increased Level of Extracellular ATP at Tumor Sites: In Vivo Imaging with Plasma Membrane Luciferase
There is growing awareness that tumour cells build up a "self-advantageous" microenvironment that reduces effectiveness of anti-tumour immune response. While many different immunosuppressive mechanisms are likely to come into play, recent evidence suggests that extracellular adenosine acting at A2A receptors may have a major role in down-modulating the immune response as cancerous tissues contain elevated levels of adenosine and adenosine break-down products. While there is no doubt that all cells possess plasma membrane adenosine transporters that mediate adenosine uptake and may also allow its release, it is now clear that most of extracellularly-generated adenosine originates from the catabolism of extracellular ATP. METHODOLOGY/PRINCIPAL FINDINGS: Measurement of extracellular ATP is generally performed in cell supernatants by HPLC or soluble luciferin-luciferase assay, thus it generally turns out to be laborious and inaccurate. We have engineered a chimeric plasma membrane-targeted luciferase that allows in vivo real-time imaging of extracellular ATP. With this novel probe we have measured the ATP concentration within the tumour microenvironment of several experimentally-induced tumours. CONCLUSIONS/SIGNIFICANCE: Our results show that ATP in the tumour interstitium is in the hundreds micromolar range, while it is basically undetectable in healthy tissues. Here we show that a chimeric plasma membrane-targeted luciferase allows in vivo detection of high extracellular ATP concentration at tumour sites. On the contrary, tumour-free tissues show undetectable extracellular ATP levels. Extracellular ATP may be crucial for the tumour not only as a stimulus for growth but also as a source of an immunosuppressive agent such as adenosine. Our approach offers a new tool for the investigation of the biochemical composition of tumour milieu and for development of novel therapies based on the modulation of extracellular purine-based signalling
Nuclear spin pair coherence in diamond for atomic scale magnetometry
The nitrogen-vacancy (NV) centre, as a promising candidate solid state system
of quantum information processing, its electron spin coherence is influenced by
the magnetic field fluctuations due to the local environment. In pure diamonds,
the environment consists of hundreds of C-13 nuclear spins randomly spreading
in several nanometers range forming a spin bath. Controlling and prolonging the
electron spin coherence under the influence of spin bath are challenging tasks
for the quantum information processing. On the other hand, for a given bath
distribution, many of its characters are encoded in the electron spin
coherence. So it is natural to ask the question: is it possible to 'decode' the
electron spin coherence, and extract the information about the bath structures?
Here we show that, among hundreds of C-13 bath spins, there exist strong
coupling clusters, which give rise to the millisecond oscillations of the
electron spin coherence. By analyzing these oscillation features, the key
properties of the coherent nuclear spin clusters, such as positions,
orientations, and coupling strengths, could be uniquely identified. This
addressability of the few-nuclear-spin cluster extends the feasibility of using
the nuclear spins in diamond as qubits in quantum computing. Furthermore, it
provides a novel prototype of single-electron spin based, high-resolution and
ultra-sensitive detector for the chemical and biological applications.Comment: 15 pages, 4 figures, Nature Nanotechnology (2011
Overexpression of microRNA-206 in the skeletal muscle from myotonic dystrophy type 1 patients
<p>Abstract</p> <p>Background</p> <p>MicroRNAs are highly conserved, noncoding RNAs involved in post-transcriptional gene silencing. They have been shown to participate in a wide range of biological processes, including myogenesis and muscle regeneration. The goal of this study is to test the hypothesis that myo-miRs (myo = muscle + miR = miRNA) expression is altered in muscle from patients affected by myotonic dystrophy type 1 (DM1), the most frequently inherited neuromuscular disease in adults. In order to gain better insights about the role of miRNAs in the DM1 pathogenesis, we have also analyzed the muscular expression of miR-103 and miR-107, which have been identified <it>in silico </it>as attractive candidates for binding to the <it>DMPK </it>mRNA.</p> <p>Methods</p> <p>To this aim, we have profiled the expression of miR-133 (miR-133a, miR-133b), miR-1, miR-181 (miR-181a, miR-181b, miR-181c) and miR-206, that are specifically induced during myogenesis in cardiac and skeletal muscle tissues. miR-103 and miR-107, highly expressed in brain, heart and muscle have also been included in this study. QRT-PCR experiments have been performed on RNA from vastus lateralis biopsies of DM1 patients (n = 7) and control subjects (n = 4). Results of miRNAs expression have been confirmed by Northern blot, whereas <it>in situ </it>hybridization technique have been performed to localize misexpressed miRNAs on muscle sections from DM1 and control individuals.</p> <p>Results</p> <p>Only miR-206 showed an over-expression in 5 of 7 DM1 patients (threshold = 2, fold change between 1.20 and 13.22, average = 5.37) compared to the control group. This result has been further confirmed by Northern blot analysis (3.37-fold overexpression, <it>R</it><sup>2 </sup>= 0.89). <it>In situ </it>hybridization localized miR-206 to nuclear site both in normal and DM1 tissues. Cellular distribution in DM1 tissues includes also the nuclear regions of centralized nuclei, with a strong signal corresponding to nuclear clumps.</p> <p>Conclusions</p> <p>This work provides, for the first time, evidences about miRNAs misexpression in DM1 muscle tissues, adding a new element in the pathogenesis of this complex genetic disease.</p
Loss of heterozygosity (LOH), malignancy grade and clonality in microdissected prostate cancer
The aim of the present study was to find out whether increasing malignancy of prostate carcinoma correlates with an overall increase of loss of heterozygosity (LOH), and whether LOH typing of microdissected tumour areas can help to distinguish between multifocal or clonal tumour development. In 47 carcinomas analysed at 25 chromosomal loci, the overall LOH rate was found to be significantly lower in grade 1 areas (2.2%) compared with grade 2 (9.4%) and grade 3 areas (8.3%, P = 0.007). A similar tendency was found for the mean fractional allele loss (FAL, 0.043 for grade 1, 0.2 for grade 2 and 0.23 for grade 3, P = 0.0004). Of 20 tumours (65%) with LOH in several microdissected areas, 13 had identical losses at 1–4 loci within two or three areas, suggesting clonal development of these areas. Markers near RB, DCC, BBC1, TP53 and at D13S325 (13q21–22) showed higher loss rates in grades 2 and 3 (between 25% and 44.4%) compared with grade 1 (0–6.6%). Tumour-suppressor genes (TSGs) near these loci might, thus, be important for tumour progression. TP53 mutations were detected in 27%, but BBC1 mutations in only 7%, of samples with LOH. Evaluation of all 25 loci in every tumour made evident that each prostate cancer has its own pattern of allelic losses. © 1999 Cancer Research Campaig
Mechanical Strain Regulates Osteoblast Proliferation through Integrin-Mediated ERK Activation
Mechanical strain plays a critical role in the proliferation, differentiation and maturation of bone cells. As mechanical receptor cells, osteoblasts perceive and respond to stress force, such as those associated with compression, strain and shear stress. However, the underlying molecular mechanisms of this process remain unclear. Using a four-point bending device, mouse MC3T3-E1 cells was exposed to mechanical tensile strain. Cell proliferation was determined to be most efficient when stimulated once a day by mechanical strain at a frequency of 0.5 Hz and intensities of 2500 µε with once a day, and a periodicity of 1 h/day for 3 days. The applied mechanical strain resulted in the altered expression of 1992 genes, 41 of which are involved in the mitogen-activated protein kinase (MAPK) signaling pathway. Activation of ERK by mechanical strain promoted cell proliferation and inactivation of ERK by PD98059 suppressed proliferation, confirming that ERK plays an important role in the response to mechanical strain. Furthermore, the membrane-associated receptors integrin β1 and integrin β5 were determined to regulate ERK activity and the proliferation of mechanical strain-treated MC3T3-E1 cells in opposite ways. The knockdown of integrin β1 led to the inhibition of ERK activity and cell proliferation, whereas the knockdown of integrin β5 led to the enhancement of both processes. This study proposes a novel mechanism by which mechanical strain regulates bone growth and remodeling
Orientia tsutsugamushi Stimulates an Original Gene Expression Program in Monocytes: Relationship with Gene Expression in Patients with Scrub Typhus
Orientia tsutsugamushi is the causal agent of scrub typhus, a public health problem in the Asia-Pacific region and a life-threatening disease. O. tsutsugamushi is an obligate intracellular bacterium that mainly infects endothelial cells. We demonstrated here that O. tsutsugamushi also replicated in monocytes isolated from healthy donors. In addition, O. tsutsugamushi altered the expression of more than 4,500 genes, as demonstrated by microarray analysis. The expression of type I interferon, interferon-stimulated genes and genes associated with the M1 polarization of macrophages was significantly upregulated. O. tsutsugamushi also induced the expression of apoptosis-related genes and promoted cell death in a small percentage of monocytes. Live organisms were indispensable to the type I interferon response and apoptosis and enhanced the expression of M1-associated cytokines. These data were related to the transcriptional changes detected in mononuclear cells isolated from patients with scrub typhus. Here, the microarray analyses revealed the upregulation of 613 genes, which included interferon-related genes, and some features of M1 polarization were observed in these patients, similar to what was observed in O. tsutsugamushi-stimulated monocytes in vitro. This is the first report demonstrating that monocytes are clearly polarized in vitro and ex vivo following exposure to O. tsutsugamushi. These results would improve our understanding of the pathogenesis of scrub typhus, during which interferon-mediated activation of monocytes and their subsequent polarization into an M1 phenotype appear critical. This study may give us a clue of new tools for the diagnosis of patients with scrub typhus
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