Aplicación de las propiedades nutracéuticas y estabilizantes del mesocarpio y exocarpio del maracuyá en la obtención de una bebida natural de chinola-piña

Introducción: este estudio consistió en el desarrollo de una bebida natural de maracuyá-piña, utilizando el exocarpio, mesocarpio y endocarpio del maracuyá como estabilizante y enriquecedor nutritivo de la bebida, convirtiéndolo así en un producto nutracéutico. El mesocarpio del maracuyá puede ser aprovechado por los macronutrientes y micronutrientes que contiene, por sus pigmentos, y por el contenido de varios nutrientes con propiedades terapéuticas. Material y método: para lograr este objetivo se prepararon pulpas especiales tratadas con biomoléculas que permiten desagregar el material vegetal del cual están compuestas las frutas dejando disponible todos los nutrientes que contiene. Resultado: de este proceso se obtuvo un jugo estable en su turbidez con un color y olor bastante acentuado, relacionado con las frutas que fueron utilizadas en la investigación. Además, se obtuvo un jugo en donde los nutrientes y moléculas bioactivas atrapadas en el mesocarpio y en el pericarpio de la fruta ahora están disponibles para su inmediata absorción en el organismo humano. Conclusión: el producto tratado con biocatalizador presentó mayor estabilidad y mayores propiedades nutracéuticas que el jugo sin ese tratamiento

Representaciones e imágenes de la diversidad cultural: Reflexiones a partir de un estudio exploratorio con profesores de escuelas multiculturales del suroeste de Andalucía

En este artículo se exponen las conclusiones de un estudio preliminar de carácter exploratorio realizado en escuelas multiculturales de la provincia de Huelva (Suroeste de España). Tras el establecimiento de un marco conceptual y la descripción analítica de la evolución del fenómeno de la inmigración en sus cifras fundamentales, se presentan los resultados de un estudio de campo, basado en entrevistas al profesorado y a otros actores escolares relevantes, de las que se extraen diferentes imágenes y estereotipos sobre el desarrollo educativo de niños y niñas inmigrantes. Por último, se sugieren algunas líneas de profundización en futuros estudios

Representations and images of cultural diversity: insights from an exploratory study with multicultural schools teachers in Southwestern Andalusia

En este artículo se exponen las conclusiones de un estudio preliminar de carácter exploratorio realizado en escuelas multiculturales de la provincia de Huelva (Suroeste de España). Tras el establecimiento de un marco conceptual y la descripción analítica de la evolución del fenómeno de la inmigración en sus cifras fundamentales, se presentan los resultados de un estudio de campo, basado en entrevistas al profesorado y a otros actores escolares relevantes, de las que se extraen diferentes imágenes y estereotipos sobre el desarrollo educativo de niños y niñas inmigrantes. Por último, se sugieren algunas líneas de profundización en futuros estudios.This article presents the findings of an exploratory study developed in multicultural schools in the province of Huelva (SW Spain). Following a conceptual and analytical description of the evolution of immigration, we present the results of a field study, conducted through interviews with teachers and other important school actors. From them we have extracted different images and stereotypes about the educational development of immigrant children. Finally, this article suggests new research proposals in future studies.Grupo FORCE (HUM-386). Departamento de Didáctica y Organización Escolar de la Universidad de Granad

Wax worm saliva and the enzymes therein are the key to polyethylene degradation by Galleria mellonella

Plastic degradation by biological systems with re-utilization of the by-products could be a future solution to the global threat of plastic waste accumulation. Here, we report that the saliva of Galleria mellonella larvae (wax worms) is capable of oxidizing and depolymerizing polyethylene (PE), one of the most produced and sturdy polyolefin-derived plastics. This effect is achieved after a few hours’ exposure at room temperature under physiological conditions (neutral pH). The wax worm saliva can overcome the bottleneck step in PE biodegradation, namely the initial oxidation step. Within the saliva, we identify two enzymes, belonging to the phenol oxidase family, that can reproduce the same effect. To the best of our knowledge, these enzymes are the first animal enzymes with this capability, opening the way to potential solutions for plastic waste management through bio-recycling/up-cycling

Guia d’identificació del plàncton

68 pages, figuresSota les onades de l’oceà es belluguen una immensitat d’organismes. Molts els coneixem però, t’has imaginat mai que alguns arribin a ser tan petits que no poden ser vistos a ull nu? Submergeix-te en el mar d’espècies que es deixen portar pel vaivé de les onades. Descobriràs el plàncton, un món fascinant!Un projecte de l’Institut de Ciències del Mar (ICM-CSIC) i l’Escola del Mar (Ajuntament de Badalona), amb la col·laboració de la Fundación Española para la Ciencia y la Tecnología (FECYT) - Ministerio de Ciencia e Innovación, i amb el suport institucional de l’acreditació AEI ‘Severo Ochoa Centre of Excellence’ (CEX2019-000928-S)Peer reviewe

Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients

Introduction: There are limited findings available on KIT-negative GIST-like (KNGL) population. Also, KIT expression may be post-transcriptionally regulated by miRNA221 and miRNA222. Hence, the aim of this study is to characterize KNGL population, by differential gene expression, and to analyze miRNA221/222 expression and their prognostic value in KNGL patients. Methods: KIT, PDGFRA, DOG1, IGF1R, MIR221 and MIR222 expression levels were determined by qRT-PCR. We also analyzed KIT and PDGFRA mutations, DOG1 expression, by immunohistochemistry, along with clinical and pathological data. Disease-free survival (DFS) and overall survival (OS) differences were calculated using Log-rank test. Results: Hierarchical cluster analyses from gene expression data identified two groups: group I had KIT, DOG1 and PDGFRA overexpression and IGF1R underexpression and group II had overexpression of IGF1R and low expression of KIT, DOG1 and PDGFRA. Group II had a significant worse OS (p = 0.013) in all the series, and showed a tendency for worse OS (p = 0.11), when analyzed only the localized cases. MiRNA222 expression was significantly lower in a control subset of KIT-positive GIST (p < 0.001). OS was significantly worse in KNGL cases with higher expression of MIR221 (p = 0.028) or MIR222 (p = 0.014). Conclusions: We identified two distinct KNGL subsets, with a different prognostic value. Increased levels of miRNA221/222, which are associated with worse OS, could explain the absence of KIT protein expression of most KNGL tumors

Physical interactions between MCM and Rad51 facilitate replication fork lesion bypass and ssDNA gap filling by non-recombinogenic functions

The minichromosome maintenance (MCM) helicase physically interacts with the recombination proteins Rad51 and Rad52 from yeast to human cells. We show, in Saccharomyces cerevisiae, that these interactions occur within a nuclease-insoluble scaffold enriched in replication/repair factors. Rad51 accumulates in a MCM- and DNA-binding-independent manner and interacts with MCMhelicases located outside of the replication origins and forks. MCM, Rad51, and Rad52 accumulate in this scaffold in G1 and are released during the S phase. In the presence of replication-blocking lesions, Cdc7 prevents their release from the scaffold, thus maintaining the interactions. We identify a rad51 mutant that is impaired in its ability to bind to MCM but not to the scaffold. This mutant is proficient in recombination but partially defective in single-stranded DNA (ssDNA) gap filling and replication fork progression through damaged DNA. Therefore, cells accumulate MCM/Rad51/Rad52 complexes at specific nuclear scaffolds in G1 to assist stressed forks through non-recombinogenic functions.Cancer Signaling networks and Molecular Therapeutic

Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients

Introduction: there are limited findings available on KIT-negative GIST-like (KNGL) population. Also, KIT expression may be post-transcriptionally regulated by miRNA221 and miRNA222. Hence, the aim of this study is to characterize KNGL population, by differential gene expression, and to analyze miRNA221/222 expression and their prognostic value in KNGL patients. Methods: KIT, PDGFRA, DOG1, IGF1R, MIR221 and MIR222 expression levels were determined by qRT-PCR. We also analyzed KIT and PDGFRA mutations, DOG1 expression, by immunohistochemistry, along with clinical and pathological data. Disease-free survival (DFS) and overall survival (OS) differences were calculated using Log-rank test. Results: hierarchical cluster analyses from gene expression data identified two groups: group I had KIT, DOG1 and PDGFRA overexpression and IGF1R underexpression and group II had overexpression of IGF1R and low expression of KIT, DOG1 and PDGFRA. Group II had a significant worse OS (p = 0.013) in all the series, and showed a tendency for worse OS (p = 0.11), when analyzed only the localized cases. MiRNA222 expression was significantly lower in a control subset of KIT-positive GIST (p < 0.001). OS was significantly worse in KNGL cases with higher expression of MIR221 (p = 0.028) or MIR222 (p = 0.014). Conclusions: we identified two distinct KNGL subsets, with a different prognostic value. Increased levels of miRNA221/222, which are associated with worse OS, could explain the absence of KIT protein expression of most KNGL tumors

THOR is a targetable epigenetic biomarker with clinical implications in breast cancer

Breast cancer (BC) is the most frequently diagnosed cancer and a leading cause of death among women worldwide. Early BC is potentially curable, but the mortality rates still observed among BC patients demon‑ strate the urgent need of novel and more efective diagnostic and therapeutic options. Limitless self-renewal is a hallmark of cancer, governed by telomere maintenance. In around 95% of BC cases, this process is achieved by telom‑ erase reactivation through upregulation of the human telomerase reverse transcriptase (hTERT). The hypermethylation of a specifc region within the hTERT promoter, termed TERT hypermethylated oncological region (THOR) has been associated with increased hTERT expression in cancer. However, its biological role and clinical potential in BC have never been studied to the best of our knowledge. Therefore, we aimed to investigate the role of THOR as a biomarker and explore the functional impact of THOR methylation status in hTERT upregulation in BC.info:eu-repo/semantics/publishedVersio