Knapsack Cover Subject to a Matroid Constraint

We consider the Knapsack Covering problem subject to a matroid constraint. In this problem, we are given an universe U of n items where item i has attributes: a cost c(i) and a size s(i). We also have a demand D. We are also given a matroid M = (U, I) on the set U. A feasible solution S to the problem is one such that (i) the cumulative size of the items chosen is at least D, and (ii) the set S is independent in the matroid M (i.e. S is in I). The objective is to minimize the total cost of the items selected, sum_{i in S}c(i). Our main result proves a 2-factor approximation for this problem. The problem described above falls in the realm of mixed packing covering problems. We also consider packing extensions of certain other covering problems and prove that in such cases it is not possible to derive any constant factor pproximations

GC-MS analysis and In-vitro anti-diabetic activity of ethanolic extract of theAtrocarpus Heterophylus (Unriped Jack Fruit)

Objective: In the present study the ethanolic extracts of Atrocarpus Heterophylus (ETAH) were studied for Aldose reductase, alpha (α)- amylase and alpha (α)-glucosidase inhibition using an in-vitro anti diabetic and evaluate the GC-MS analysis of active compounds of ETAH. Methods: The serial extraction was carried out with a series of solvents: Petroleum ether, ethyl acetate and ethanol with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were subjected to the antidiabetic activity was assessed by employing standard in-vitro techniques. Results and discussion: The result showed ethanolic extract exhibited significant aldose reductase, α-amylase and α-glucosidase inhibitory activities with an all plant extracts respectively and well compared with standard acarbose drug.  GC-MS analysis results revealed that ETAH contain the compounds are Cyclohexanol and Nonadecene. This knowledge will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics. Conclusion: The investigation confirms that ethanolic extract exhibited highest antidiabetic activity among all extracts, Additional studies on needed for purification, characterization and structural elucidation of bioactive compounds from ethanolic extrac

Density Functions subject to a Co-Matroid Constraint

In this paper we consider the problem of finding the {\em densest} subset subject to {\em co-matroid constraints}. We are given a {\em monotone supermodular} set function $f$ defined over a universe $U$, and the density of a subset $S$ is defined to be f(S)/\crd{S}. This generalizes the concept of graph density. Co-matroid constraints are the following: given matroid \calM a set $S$ is feasible, iff the complement of $S$ is {\em independent} in the matroid. Under such constraints, the problem becomes \np-hard. The specific case of graph density has been considered in literature under specific co-matroid constraints, for example, the cardinality matroid and the partition matroid. We show a 2-approximation for finding the densest subset subject to co-matroid constraints. Thus, for instance, we improve the approximation guarantees for the result for partition matroids in the literature

EF hand-mediated Ca2+- and cGMP-signaling in photoreceptor synaptic terminals

Photoreceptors, the light-sensitive receptor neurons of the retina, receive and transmit a plethora of visual informations from the surrounding world. Photoreceptors capture light and convert this energy into electrical signals that are conveyed to the inner retina. For synaptic communication with the inner retina, photoreceptors make large active zones that are marked by synaptic ribbons. These unique synapses support continuous vesicle exocytosis that is modulated by light-induced, graded changes of membrane potential. Synaptic transmission can be adjusted in an activity-dependent manner, and at the synaptic ribbons, Ca2+- and cGMP-dependent processes appear to play a central role. EF-hand-containing proteins mediate many of these Ca2+- and cGMP-dependent functions. Since continuous signaling of photoreceptors appears to be prone to malfunction, disturbances of Ca2+- and cGMP-mediated signaling in photoreceptors can lead to visual defects, retinal degeneration (rd), and even blindness. This review summarizes aspects of signal transmission at the photoreceptor presynaptic terminals that involve EF-hand-containing Ca2+-binding proteins

Larvicidal, Histopathological, Antibacterial Activity of Indigenous Fungus Penicillium sp. Against Aedes aegypti L and Culex quinquefasciatus (Say) (Diptera: Culicidae) and Its Acetylcholinesterase Inhibition and Toxicity Assessment of Zebrafish (Danio rerio)

Fungal metabolites are considered to be most efficient tools to overcome the issues related to insecticide resistance and environmental pollution. The present study focus on the evaluation of the mosquito larvicidal efficacy of metabolites of seven indigenous fungal isolates (Penicillium sp. Aspergillus niger, A. flavus, A. parasiticus, Rhizopus sp. Mucor sp. and Aspergillus sp.) on the larvae of Aedes aegypti and Culex quinquefasciatus under the laboratory condition. The preliminary screening of the isolate, Penicillium sp. showed better larvicidal effect when compared to other fungi. The fungus was grown on Potato Dextrose Broth (PDB) in the laboratory (at 25°C) and maintained in the relative humidity (at 76 ± 4% for 15 days). Larvicidal potency of mycelial ethyl acetate extract (MEAE) of Penicillium sp. was performed against 1st to 4th instars larvae of Ae. aegypti and Cx. quinquefasciatus using four different concentrations (100, 200, 300, and 500 μg/ml) that showed better larval mortality values (μg/ml) of LC50 = 6.554, 5.487, 6.874, 6.892, and the LC90 = 11.486, 10.366, 12.879, 13.865 for Ae. aegypti and LC50 = 7.000, 13.943, 18.129, 25.212 and the LC90 = 12.541, 23.761, 30.923, 41.696 for Cx. quinquefasciatus. Exposure of metabolite to larvae resulted in behavior changes i.e., excitation, up and down with aggressive movement, anal papillae biting behavior. Further, the larvae treated with Penicillium sp. metabolite exhibited significant reduction in the levels of acetylcholinesterase. The 4th instar mosquito larvae treated with the 500 μg/ml mycelia extract showed severe histological damages. During the antibacterial analysis of Penicillium sp.- mycelium the maximum growth inhibition zone was recorded in Shigella dysenteriae (31.2 mm) and Klebsiella pneumoniae (31.1 mm) followed by others. In addition, to check the toxicity of Penicillium sp. MEAE against embryos of Zebrafish, a model system, using different concentrations of metabolites (1.0, 0.5, 0.125 mg/ml, 30, 3.0, and 0.5 μg/ml) and life-stage parameters were observed at 124 hpf. Furthermore, the Fourier Transformed Infrared and GCMS spectrum analysis of mycelium reflected several chemical compounds. The outcome of the study clearly shows that Penicillium sp. metabolites could serve as an ideal eco-friendly, single-step and inexpensive source for the control of Ae. aegypti and Cx. quinquefasciatus larvae

Clinico-pathological association of delineated miRNAs in uveal melanoma with monosomy 3/Disomy 3 chromosomal aberrations

PURPOSE: To correlate the differentially expressed miRNAs with clinico-pathological features in uveal melanoma (UM) tumors harbouring chromosomal 3 aberrations among South Asian Indian cohort. METHODS: Based on chromosomal 3 aberration, UM (n = 86) were grouped into monosomy 3 (M3; n = 51) and disomy 3 (D3; n = 35) by chromogenic in-situ hybridisation (CISH). The clinico-pathological features were recorded. miRNA profiling was performed in formalin fixed paraffin embedded (FFPE) UM samples (n = 6) using Agilent, Human miRNA microarray, 8x15KV3 arrays. The association between miRNAs and clinico-pathological features were studied using univariate and multivariate analysis. miRNA-gene targets were predicted using Target-scan and MiRanda database. Significantly dys-regulated miRNAs were validated in FFPE UM (n = 86) and mRNAs were validated in frozen UM (n = 10) by qRT-PCR. Metastasis free-survival and miRNA expressions were analysed by Kaplen-Meier analysis in UM tissues (n = 52). RESULTS: Unsupervised analysis revealed 585 differentially expressed miRNAs while supervised analysis demonstrated 82 miRNAs (FDR; Q = 0.0). Differential expression of 8 miRNAs: miR-214, miR-149*, miR-143, miR-146b, miR-199a, let7b, miR-1238 and miR-134 were studied. Gene target prediction revealed SMAD4, WISP1, HIPK1, HDAC8 and C-KIT as the post-transcriptional regulators of miR-146b, miR-199a, miR-1238 and miR-134. Five miRNAs (miR-214, miR146b, miR-143, miR-199a and miR-134) were found to be differentially expressed in M3/ D3 UM tumors. In UM patients with liver metastasis, miR-149* and miR-134 expressions were strongly correlated. CONCLUSION: UM can be stratified using miRNAs from FFPE sections. miRNAs predicting liver metastasis and survival have been identified. Mechanistic linkage of de-regulated miRNA/mRNA expressions provide new insights on their role in UM progression and aggressiveness

Pedagogic inclusion in professional education : challenges and possibilities?

This thesis describes a qualitative, interview-based case study of the experiences of adult female learners accessing/reaccessing Higher Education following a Widening Participation (WP) route on a part-time Physiotherapy undergraduate course and an accelerated Masters course in Occupational Therapy at a single HEI. The research questioned student and staff perceptions of and attitudes to study, and to what extent the HEI had adapted its pedagogic approaches to accommodate student learning preferences. Data are drawn from discussions with sixteen participants, comprising four students and four tutors on each course. The research outcomes revealed a tension between the tutors' theoretically and ideologically driven views on teaching and learning, which included responding to diverse student needs, and the more pragmatic orientations of the students and their tutors. These were underpinned in the students' case by the financial need to gain a qualification in spite of the constraints of time and family life, and on the tutors' and institution's part by the need to attract student numbers and to meet completion targets in spite of shortages of time, staffing and space. Analysis of the responses of students and tutors draws on Bernstein's theory of recontextualisation, in which ideas such as WP undergo significant transformations first in the official policy process then when they are put into practice. The study also draws on Bernstein's account of pedagogic identities, which describe how teachers and institutions negotiate teaching and learning processes within the competing demands of market forces, mandated policy and their own local ideologies and circumstances. In its exploration of the meaning of WP and its underpinning rationales, the research suggests that the pragmatic approach of the institution has the effect of widening access in terms of course eligibility but failing to offer correspondingly inclusive pedagogies, more flexible curricula, or adequate student support services

Biomarkers of Ionizing Radiation Exposure: A Multiparametric Approach.

Humans are exposed to ionizing radiation not only through background radiation but also through the ubiquitous presence of devices and sources that generate radiation. With the expanded use of radiation in day-to-day life, the chances of accidents or misuse only increase. Therefore, a thorough understanding of the dynamic effects of radiation exposure on biological entities is necessary. The biological effects of radiation exposure on human cells depend on much variability such as level of exposure, dose rate, and the physiological state of the cells. During potential scenarios of a large-scale radiological event which results in mass casualties, dose estimates are essential to assign medical attention according to individual needs. Many attempts have been made to identify biomarkers which can be used for high throughput biodosimetry screening. In this study, we compare the results of different biodosimetry methods on the same irradiated cells to assess the suitability of current biomarkers and push forward the idea of employing a multiparametric approach to achieve an accurate dose and risk estimation

Constraints on the Redshift Evolution of the L_X-SFR Relation from the Cosmic X-Ray Backgrounds

Observations of local star forming galaxies have revealed a correlation between the rate at which galaxies form stars and their X-Ray luminosity. We combine this correlation with the most recent observational constraints on the integrated star formation rate density, and find that star forming galaxies account for 5-20% of the total soft and hard X-ray backgrounds, where the precise number depends on the energy band and the assumed average X-ray spectral energy distribution of the galaxies below ~20 keV. If we combine the L_X-SFR relation with recently derived star formation rate function, then we find that star forming galaxies whose X-ray flux falls well (more than a factor of 10) below the detection thresholds of the Chandra Deep Fields, can fully account for the unresolved soft X-ray background, which corresponds to ~6% of its total. Motivated by this result, we put limits on the allowed redshift evolution of the parameter c_X \equiv L_X/SFR, and/or its evolution towards lower and higher star formation rates. If we parametrize the redshift evolution of c_X ~ (1+z)^b, then we find that b \leq 1.3 (95% CL). On the other hand, the observed X-ray luminosity functions (XLFs) of star forming galaxies indicate that c_X may be increasing towards higher redshifts and/or higher star formation rates at levels that are consistent with the X-ray background, but possibly at odds with the locally observed L_X-SFR relation.Comment: Accepted to MNRAS after minor revisions. 12 pages, 8 figure