Double-Blinded, Vehicle-Controlled Proof of Concept Study to Investigate the Recurrence of Inflammatory and Noninflammatory Acne Lesions Using Tretinoin Gel (Microsphere) 0.04% in Male Patients after Oral Isotretinoin Use

Background. Although isotretinoin orally is commonly used for moderate to severe or scarring acne, it is not a cure. Unfortunately recurrence is unpredictable and varies within the acne population. Objectives. Using a proof of concept study, determine the recurrence of acne after isotretinoin use in male patients. Methods. Twenty males aged 18–45 years old were enrolled. Subjects successfully completed a treatment of acne vulgaris with oral isotretinoin (120–150 mg/kg/course). Subjects were randomized 1 to 1. The study duration was 24 weeks. The primary endpoint measured was the absolute change in lesion counts from baseline to weeks 16 and 24. Local tolerability assessments were measured. Results. There were favorable changes in all outcomes measured. Overall, there was a 38.7% lower lesion count with tretinoin 0.04% microsphere gel use versus vehicle. The active product was well tolerated with great patient satisfaction. There were no significant safety issues. The limitations included the low number of patients enrolled, average age, and percentage of patients lost to follow-up. Conclusion. In summary, the results favored tretinoin 0.04% microsphere gel in the prevention of recurrent acne after isotretinoin use in male patients over 18 years old over a six-month period

Foreshore beach sand as a reservoir and source of total phosphorus in Lake Ontario

<p>Many regions around the Great Lakes have been designated Areas of Concern as a result of consistent water quality problems from pollutants like phosphorus and <i>Escherichia coli</i>, which cause eutrophication, beach postings and Beneficial Use Impairments. While foreshore beach sand is a potential reservoir for <i>E. coli</i>, there is less understanding of whether it might also be a reservoir and source of phosphorus for adjacent beach waters. We measured levels of <i>E. coli,</i> total phosphorus and soluble reactive phosphorus at Sunnyside and Rouge Beaches in the Toronto and Region Area of Concern, and stormwater outfalls in the adjacent Humber and Rouge Rivers within their beachsheds. Additionally, we used microbial source tracking assays to detect human and gull fecal contamination. Soluble reactive phosphorus concentrations were highest in stormwater outfalls, with concentrations as high as 556 µg l⁻¹ at an outfall in the Sunnyside beachshed, and 4780 µg l⁻¹ at an outfall in the Rouge beachshed. In contrast, the highest total phosphorus concentrations were typically found in foreshore beach sand pore water and were more associated with gull fecal contamination. Beach sand total phosphorus levels were as high as 10,600 µg l⁻¹ at Sunnyside Beach, although the highest total phosphorus concentration measured (25,600 µg l⁻¹) was in a Rouge River outfall. Concentrations of total phosphorus in outfalls were significantly correlated with concentrations of <i>E. coli</i> in both beachsheds and the human microbial source tracking marker in the Sunnyside beachshed outfalls. These results indicate that stormwater outfalls with sewage cross-contamination can deliver high concentrations of total phosphorus, soluble reactive phosphorus and fecal bacterial contamination to associated beachsheds. Further, similar to <i>E. coli</i>, foreshore beach sand can act as a reservoir of total phosphorus and a source for adjacent water bodies via wave action or groundwater discharge. High phosphorus inputs from beach sand could contribute localized changes to microbial communities and unique eutrophication effects along beach shorelines.</p

Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve

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