Predicting insertion: external force application onto cells allow nanowire arrays to insert into cytosol

The use of vertical nanowires in biosensing application is limited by the extend of how efficient nanowires can reach the intracellular domains. Studies have found nanowire insertion to be successful through single nanowire experiments with high force pr. nanowire, using AFM. This is inadequate for producing high throughput analysis of several cells with multiple nanowires inserting in each cell. Here, we present a model for prediction of the nanowire insertion rates, when centrifugating cell samples down onto arrays of vertical indium arsenide nanowires, with a diameter of 100 nm, height of 3 μm and spacing of 3-5 μm. The model utilizes sedimentation rate of cells in conjunction with the centrifugal force field applied to objects subjected to centrifugation. Various conditions tested with the model, including lowering the temperature of the experiment from room temperature to 4 °C, show that cell viability is not negatively affected by any of the conditions. The insertion rate has been shown to be largely affected by the density of the nanowire array substrate, with a lower density resulting in a higher NW insertion rate, as the model predicts. The percentage of cells with at least one successful nanowire insertion is, however, oppositely affected by density, as a higher density results in larger percentage of cells with successful insertions

Response Patterns in Finger and Central Body Skin Temperatures Under Mild Whole Body Cooling

INTRODUCTION The actual heat loss may be underestimated especially in studies employing mild whole-body cooling, if AVA-rich distal areas are not taken into account. In the present report, we illustrate the skin temperature response pattern in fingers (rich in AVAs) to transient whole-body cooling as compared to non-acral body sites (without AVAs). METHODS Eight men participated in the study. During the test the subjects were dressed in shorts, socks and shoes and stayed seated with the arms on insulated supports at heart level. The air temperature of 32 °C was after 25 minutes gradually reduced to 13 °C (0.2 °C/min). Core, finger (sulcus lateral to the nailbed) and non-acral skin (8 points) temperatures were measured. RESULTS During cooling the mean skin temperature in all subjects decreased at a similar rate. Higher variation in the end of the cooling could be explained by differences in body fat (R2=0.902). Simultaneously, the finger cooling could start with up to about 1 hour difference in different subjects. DISCUSSION AND CONCLUSION Mean skin temperature did not give any idea on when the subjects left thermal neutrality. It is strongly recommended to measure finger (or toe) temperatures when maintaining the thermal comfort of the subjects in dynamic conditions is important

The interacting nature of dwarf galaxies hosting superluminous supernovae

(Abridged) Type I superluminous supernovae (SLSNe I) are rare, powerful explosions whose mechanism and progenitors remain elusive. SLSNe I show a preference for low-metallicity, actively star-forming dwarf galaxies. We investigate whether the hosts of SLSNe I show increased evidence for interaction. We use a sample of 42 SLSN I images obtained with $\textit{HST}$ and measure the number of companion galaxies by counting the objects detected within a given radius from the host. As a comparison, we used two Monte Carlo-based methods to estimate the expected average number of companion objects in the same images, as well as a sample of 32 galaxies that have hosted long gamma-ray bursts (GRBs). About 50% of SLSN I hosts have at least one major companion (within a flux ratio of 1:4) within 5 kpc. The average number of major companions per SLSN I host galaxy is $0.70^{+0.19}_{-0.14}$. Our Monte Carlo comparison methods yield a lower number of companions for random objects of similar brightness in the same image or for the SLSN host after randomly redistributing the sources in the same image. The Anderson-Darling test shows that this difference is statistically significant independent of the redshift range. The same is true for the projected distance distribution of the companions. The SLSN I hosts are, thus, found in areas of their images, where the object number density is greater than average. SLSN I hosts have more companions than GRB hosts ($0.44^{+0.25}_{-0.13}$ companions per host distributed over 25% of the hosts) but the difference is not statistically significant. The difference between their separations is, however, marginally significant. The dwarf galaxies hosting SLSNe I are often part of interacting systems. This suggests that SLSNe I progenitors are formed after a recent burst of star formation. Low metallicity alone cannot explain this tendency.Comment: Accepted for publication in A&A. In v2 replaced graphs with higher quality PDF version

Effects of Cooling During Exercise on Thermoregulatory Responses of Men With Paraplegia.

BACKGROUND: People with spinal cord injury (SCI) have an altered afferent input to the thermoregulatory center, resulting in a reduced efferent response (vasomotor control and sweating capacity) below the level of the lesion. Consequently, core body temperature rises more rapidly during exercise in individuals with SCI compared with people who are able-bodied. Cooling strategies may reduce the thermophysiological strain in SCI. OBJECTIVE: The aim of this study was to examine the effects of a cooling vest on the core body temperature response of people with a thoracic SCI during submaximal exercise. METHODS: Ten men (mean age=44 years, SD=11) with a thoracic lesion (T4-T5 or below) participated in this randomized crossover study. Participants performed two 45-minute exercise bouts at 50% maximal workload (ambient temperature 25°C), with participants randomized to a group wearing a cooling vest or a group wearing no vest (separate days). Core body temperature and skin temperature were continuously measured, and thermal sensation was assessed every 3 minutes. RESULTS: Exercise resulted in an increased core body temperature, skin temperature, and thermal sensation, whereas cooling did not affect core body temperature. The cooling vest effectively decreased skin temperature, increased the core-to-trunk skin temperature gradient, and tended to lower thermal sensation compared with the control condition. LIMITATIONS: The lack of differences in core body temperature among conditions may be a result of the relative moderate ambient temperature in which the exercise was performed. CONCLUSIONS: Despite effectively lowering skin temperature and increasing the core-to-trunk skin temperature gradient, there was no impact of the cooling vest on the exercise-induced increase in core body temperature in men with low thoracic SCI

Psykopati og psykopatbehandling

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