EXPERIMENTAL EVALUATION OF ANTIDEPRESSANT ACTIVITY OF AQUEOUS AND CHLOROFORM LEAF AND SHOOT EXTRACTS OF EICCHORNIA CRASSIPES LINN IN MICE

Objective: To investigate antidepressant activity of aqueous and chloroform extract of Eicchornia crassipes plant leaves and shoots in mice.Methods: The antidepressant activity of aqueous and chloroform extract of Eicchornia crassipes plant leaves and shoots were tested by forced swimtest (FST) and tail suspension test (TST) in albino mice and the results were compared for the both extracts. Imipramine was used as the standardrug for comparison. Results: Phytochemical screening showed presence of carbohydrates, alkaloids, flavanoids, steroids, saponins, amino acids, gums and mucilage.aqueous extract of Eicchornea crassipes (AEEC) and chloroform extract of E. crassipes (CEEC) did not produce any lethal effect even upto 2000 mg/kp.o during acute oral toxicity study. In FST and TST, CEEC showed diminution of duration of immobility time in 200 mg/kg but not in 100 mg/kg. Conclusion: From the above finding concluding that, shortening of immobility time in the FST and TST indicating, CEEC showed more antidepressantactivity acting either by the enhancement of central 5-HT or catecholamine neurotransmission compared to AEEC in mice.Keywords: Eicchornia crassipes, Aqueous extract of Eicchornia crassipes, Chloroform extract of Eicchornia crassipes, Forced swim test,Tail suspension test

PHARMACOLOGICAL EVALUATION OF ANTIDEPRESSANT ACTIVITY OF AQUEOUS EXTRACT OF ROSA CENTIFOLIA PETALS IN MICE

ABSTRACT To study the antidepressant activity with aqueous extract of Rosa centifolia. The extract was primarily subjected for preliminary phytochemical investigation and for Maximum Tolerance Dose (MTD). Antidepressant activity was evaluated in various animal models like Forced swim test, Tail suspension test, Apomorphine induced hypothermia and 5-HTP potentiation of head twitches in mice. The aqueous extract of Rosa centifolia was positively identified with carbohydrates, tannins, proteins, amino acids, alkaloids, flavonoids, flavanone, glycosides and phenolic compounds. The extract was subjected for maximum tolerance dose upto the dose level of 2000mg/kg has not produced any mortality. The extract of Rosa centifolia showed significant antidepressant activity at high dose (100 mg/kg) in Tail suspension test, Forced swim test and Potentiation of 5-HTP induced head twitches. The extract didn't antagonized the hypothermia induced by apomorphine