Further Investigations of a Mesospheric Inversion Layer Observed in the ALOHA-93 Campaign

Temperature and wind data obtained from a Na wind/temperature lidar operated by the University of Illinois group during the Airborne Lidar and Observations of the Hawaiian Airglow (ALOHA-93) Campaign, previously analyzed by Huang et al. [1998] using an isothermal Brunt-Va¨isa¨la¨ frequency, have been reexamined to include temperature gradients in the calculation of the Richardson number. In the previous analysis using the isothermal Brunt-Va¨isa¨la¨ frequency the existence of convective instability could not be assessed. New analysis shows that the nonisothermal Richardson number preserves some features found previously, with some striking differences noticable at times between 0900 and 1030 UT. The nonisothermal Richardson number becomes negative as early as 0930 UT, indicating conditions conducive to the development of convective instability and turbulence. The possibility that turbulence could exist at times earlier than previously thought explains more satisfactorily the large temperature increase observed before 1000 UT

STUDIES OF THE LINKAGE AND BONDING OF TRIATOMICS IN TRANSITION METAL COMPLEXES. PART 2. NCS-COMPLEXES

ABSTRACT The geometries of NCS -coordinated to transition metals range over N-or S-linkage, N-or Sbridge, end-to-end bridge and possibly other arrangements. In the coordination of NCS-to transition metals, various metal-to-ligand angles have also been reported

Mesodermal fate decisions of a stem cell: the Wnt switch

Stem cells are a powerful resource for cell-based transplantation therapies in osteodegenerative disorders, but before some kinds of stem cells can be applied clinically, several aspects of their expansion and differentiation need to be better controlled. Wnt molecules and members of the Wnt signaling cascade have been ascribed a role in both these processes in vitro as well as normal development in vivo. However some results are controversial. In this review we will present the hypothesis that both canonical and non-canonical signaling are involved in mesenchymal cell fate regulation, such as adipogenesis, chondrogenesis and osteogenesis, and that in vitro it is a timely switch between the two that specifies the identity of the differentiating cell. We will specifically focus on the in vitro differentiation of adipocytes, chondrocytes and osteoblasts contrasting embryonic and mesenchymal stem cells as well as the role of Wnts in mesenchymal fate specification during embryogenesis

Safety and efficacy of fluoxetine on functional outcome after acute stroke (AFFINITY): a randomised, double-blind, placebo-controlled trial

Background Trials of fluoxetine for recovery after stroke report conflicting results. The Assessment oF FluoxetINe In sTroke recoverY (AFFINITY) trial aimed to show if daily oral fluoxetine for 6 months after stroke improves functional outcome in an ethnically diverse population. Methods AFFINITY was a randomised, parallel-group, double-blind, placebo-controlled trial done in 43 hospital stroke units in Australia (n=29), New Zealand (four), and Vietnam (ten). Eligible patients were adults (aged ≥18 years) with a clinical diagnosis of acute stroke in the previous 2–15 days, brain imaging consistent with ischaemic or haemorrhagic stroke, and a persisting neurological deficit that produced a modified Rankin Scale (mRS) score of 1 or more. Patients were randomly assigned 1:1 via a web-based system using a minimisation algorithm to once daily, oral fluoxetine 20 mg capsules or matching placebo for 6 months. Patients, carers, investigators, and outcome assessors were masked to the treatment allocation. The primary outcome was functional status, measured by the mRS, at 6 months. The primary analysis was an ordinal logistic regression of the mRS at 6 months, adjusted for minimisation variables. Primary and safety analyses were done according to the patient's treatment allocation. The trial is registered with the Australian New Zealand Clinical Trials Registry, ACTRN12611000774921. Findings Between Jan 11, 2013, and June 30, 2019, 1280 patients were recruited in Australia (n=532), New Zealand (n=42), and Vietnam (n=706), of whom 642 were randomly assigned to fluoxetine and 638 were randomly assigned to placebo. Mean duration of trial treatment was 167 days (SD 48·1). At 6 months, mRS data were available in 624 (97%) patients in the fluoxetine group and 632 (99%) in the placebo group. The distribution of mRS categories was similar in the fluoxetine and placebo groups (adjusted common odds ratio 0·94, 95% CI 0·76–1·15; p=0·53). Compared with patients in the placebo group, patients in the fluoxetine group had more falls (20 [3%] vs seven [1%]; p=0·018), bone fractures (19 [3%] vs six [1%]; p=0·014), and epileptic seizures (ten [2%] vs two [<1%]; p=0·038) at 6 months. Interpretation Oral fluoxetine 20 mg daily for 6 months after acute stroke did not improve functional outcome and increased the risk of falls, bone fractures, and epileptic seizures. These results do not support the use of fluoxetine to improve functional outcome after stroke

Further Investigations of a Mesospheric Inversion Layer Observed in the ALOHA-93 Campaign

Temperature and wind data obtained from a Na wind/temperature lidar operated by the University of Illinois group during the Airborne Lidar and Observations of the Hawaiian Airglow (ALOHA-93) Campaign, previously analyzed by Huang et al. [1998] using an isothermal Brunt-Va¨isa¨la¨ frequency, have been reexamined to include temperature gradients in the calculation of the Richardson number. In the previous analysis using the isothermal Brunt-Va¨isa¨la¨ frequency the existence of convective instability could not be assessed. New analysis shows that the nonisothermal Richardson number preserves some features found previously, with some striking differences noticable at times between 0900 and 1030 UT. The nonisothermal Richardson number becomes negative as early as 0930 UT, indicating conditions conducive to the development of convective instability and turbulence. The possibility that turbulence could exist at times earlier than previously thought explains more satisfactorily the large temperature increase observed before 1000 UT

Cancer-Associated Fibroblasts from Hepatocellular Carcinoma Promote Malignant Cell Proliferation by HGF Secretion

<div><p>Cancer-associated fibroblasts (CAFs) are reported to support tumorigenesis by stimulating angiogenesis, cancer cell proliferation, and invasion in most solid tumors. However, the roles of CAFs in the liver cancer microenvironment have not been thoroughly studied. In our previous study, we successfully isolated CAFs from hepatocellular carcinoma (HCC) (H-CAFs) and proved that H-CAFs suppressed the activation of NK cells and thereby created favorable conditions for HCC progression. In our present study, we found that the proliferation of MHCC97L and Hep3B cells was significantly promoted by treatment with conditioned medium from H-CAFs. Pathological analysis also revealed that H-CAFs increased the proportion of Ki-67 (+) malignant cells and prevented them from undergoing necrosis. Moreover, the concentration of hepatocyte growth factor (HGF) cytokine in the conditioned medium of H-CAFs was higher than conditioned medium from normal skin fibroblasts (NSFs). Anti-HGF significantly reduced the proliferation-promoting capability of H-CAFs. In addition, we found that the abundance of H-CAFs correlated positively with tumor size. These results indicate that H-CAFs are an important factor for promoting the growth of HCC <i>in vitro</i> and <i>in vivo</i>, and that HGF plays a key role in HCC proliferation induced by H-CAFs.</p></div