Studies of the growth and tropisms of aspergillus giganteus and other fungi

The tall conidiophores of Aspergillus giganteus are produced by large foot cells formed by the mycelium. They only continue to grow when exposed to light of wavelength below 530 mp. The photochemical reaction involved in the inductive process is a 'low energy' reaction, i.e. light acts as a 'triggering' mechanism. The response to light is localised in the areas of the mycelium actually exposed to the radiation. The density of tall conidiophore production, but not their height, is influenced by the concentration of glucose in the medium. The tall conidiophores have the usual type of growth curve; they reach their maximum rate of elongation when they are c. 3 mm tall. Elongation is confined to the terminal portion of the conidiophore. At low light intensities the conidiophores show regular oscillations along their length. The rate of conidiophore growth is influenced by light intensity; they do not show 'light growth reaction' of the kind found in Phycomyces sporangiophores. The conidiophores only continue to elongate in light when the relative humidity is close to 100%. There is a rapid reduction in the rate of conidiophore growth during vesicle formation; no further growth takes place after the vesicle has been formed. The optimum temperature for mycelial growth is c. 30 G, while the optimum temperature for maximum conidiophore height is 20 - 23 G.The presence in the medium of the riboflavin inhibitors, 1-lyxoflavin and mepacrine, or the p-carotene inhibitor, diphenylamine, did not inhibit the photoinductive process. The presence of gaseous carbon dioxide is not essential either during the photoinductive process or for conidiophore growth. Free oxygen is not required during the actual period of photoinduction but is required for conidiophore growth. Photoinduction in the absence of gaseous oxygen is less effective in inducing carotenogenesis than photo induction in the presence of oxygen. The conidiophores are strongly positive phototropic but do appear to be sensitive to the stimulus of gravity. There is a reversal of the normal phototropic response in ultra-violet radiation of 280 mp. and when the conidiophores are submerged in liquid paraffin

Zeros of slice functions and polynomials over dual quaternions

This work studies the zeros of slice functions over the algebra of dual quaternions and it comprises applications to the problem of factorizing motion polynomials. The class of slice functions over an alternative $*$-algebra $A$ was defined by Ghiloni and Perotti in 2011, extending the class of slice regular functions introduced by Gentili and Struppa in 2006. Both classes strictly include the polynomials over $A$. We focus on the case when $A$ is the algebra of dual quaternions $\mathbb{DH}$. The specific properties of this algebra allow a full characterization of the zero sets, which is not available over general alternative $*$-algebras. This characterization sheds some light on the study of motion polynomials over $\mathbb{DH}$, introduced by Heged\"us, Schicho, and Schr\"ocker in 2013 for their relevance in mechanism science.Comment: 37 pages, to appear in Trans. Amer. Math. So

Spatial organisation of ecologically relevant high order flow properties and implications for river habitat assessment

PhDThe turbulent properties of flow in rivers are of fundamental importance to aquatic organisms yet are rarely quantified during routine river habitat assessment surveys or the design of restoration schemes due to their complex nature. This thesis uses a detailed review of the literature to highlight the various ways in which plants and animals modify the flow field, how this can deliver beneficial effects; and how turbulence can also generate threats to growth and survival. The thesis then presents the results from detailed field assessments of turbulence properties undertaken on low, intermediate and high gradient rivers to advance scientific understanding of the hydrodynamics of rivers and inform effective habitat assessment and restoration. A reach-scale comparison across sites reveals spatial variations in the relationships between turbulent parameters, emphasising the need for direct measurement of turbulence properties, while a geomorphic unit scale assessment suggests that variations in turbulence at the scale of individual roughness elements, and/or within the same broad groupings of geomorphic units (e.g. different types of pools) can have an important influence on hydraulic habitat. The importance of small-scale flow obstructions is further emphasised through analysis of the temporal dynamics of turbulence properties with changes in flow stage and vegetation growth. The highest magnitude temporal changes in turbulence properties were associated with individual boulders and vegetation patches respectively, indicating flow intensification around these sub-geomorphic unit scale features. Experimental research combining flow measurement with underwater videography reveals that more sophisticated turbulence parameters provide a better explanation of fish behaviour and habitat use under field conditions, further supporting direct measurement of turbulent properties where possible. The new insights into interactions between geomorphology, hydraulics and aquatic organisms generated by this work offer opportunities for refining habitat assessment and restoration design protocols to better integrate the important role of turbulence in generating suitable physical habitat for aquatic organisms.SMART (Science for the MAnagement of Rivers and their Tidal systems) Joint Doctorate Erasmus Mundus programme funded by the European Union

Nutritional requirements and strain heterogeneity in Ashbya gossypii

Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at pH 4.5 was observed only on complex medium. D-Glucose, glycerol and starch were utilised as carbon sources. Ethanol was produced during growth on glycerol. Conversion of xylose into xylitol demonstrates that the xylose reductase is active. Phenotypic differences between related strains were greater than expected. We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future.The financial support of Fundacao para a Ciencia e a Tecnologia (FCT), Portugal, is acknowledged, project AshByofactory PTDC/EBB-EBI/101985/2008 and grant SFRH/BD/30229/2006 to O. Ribeiro

The CannTeen study: verbal episodic memory, spatial working memory, and response inhibition in adolescent and adult cannabis users and age-matched controls

BACKGROUND: Preclinical and human studies suggest that adolescent cannabis use may be associated with worse cognitive outcomes than adult cannabis use. We investigated the associations between chronic cannabis use and cognitive function in adolescent and adult cannabis users and controls. We hypothesised user-status would be negatively associated with cognitive function and this relationship would be stronger in adolescents than adults. METHODS: As part of the 'CannTeen' project, this cross-sectional study assessed cognitive performance in adolescent cannabis users (n = 76; 16-17-year-olds), adolescent controls (n = 63), adult cannabis users (n = 71; 26-29-year-olds) and adult controls (n = 64). Users used cannabis 1-7 days/week. Adolescent and adult cannabis users were matched on cannabis use frequency (4 days/week) and time since last use (2.5 days). Verbal episodic memory (VEM) was assessed using the prose recall task, spatial working memory (SWM) was assessed using the spatial n-back task, and response inhibition was assessed with the stop-signal task. Primary outcome variables were: delayed recall, 3-back discriminability, and stop signal reaction time, respectively. RESULTS: Users had worse VEM than controls (F(1,268) = 7.423, p = 0.007). There were no significant differences between user-groups on SWM or response inhibition. Null differences were supported by Bayesian analyses. No significant interactions between age-group and user-group were found for VEM, SWM, or response inhibition. CONCLUSIONS: Consistent with previous research, there was an association between chronic cannabis use and poorer VEM, but chronic cannabis use was not associated with SWM or response inhibition. We did not find evidence for heightened adolescent vulnerability to cannabis-related cognitive impairment

Morphological Instabilities in a growing Yeast Colony: Experiment and Theory

We study the growth of colonies of the yeast Pichia membranaefaciens on agarose film. The growth conditions are controlled in a setup where nutrients are supplied through an agarose film suspended over a solution of nutrients. As the thickness of the agarose film is varied, the morphology of the front of the colony changes. The growth of the front is modeled by coupling it to a diffusive field of inhibitory metabolites. Qualitative agreement with experiments suggests that such a coupling is responsible for the observed instability of the front.Comment: RevTex, 4 pages and 3 figure

Associations between regular cannabis use and brain resting-state functional connectivity in adolescents and adults

BACKGROUND/AIM: Cannabis use is highly prevalent in adolescents; however, little is known about its effects on adolescent brain function. METHOD: Resting-state functional magnetic resonance imaging was used in matched groups of regular cannabis users (N = 70, 35 adolescents: 16-17 years old, 35 adults: 26-29 years old) and non-regular-using controls (N = 70, 35 adolescents/35 adults). Pre-registered analyses examined the connectivity of seven major cortical and sub-cortical brain networks (default mode network, executive control network (ECN), salience network, hippocampal network and three striatal networks) using seed-based analysis methods with cross-sectional comparisons between user groups and age groups. RESULTS: The regular cannabis use group (across both age groups), relative to controls, showed localised increases in connectivity only in the ECN analysis. All networks showed localised connectivity differences based on age group, with the adolescents generally showing weaker connectivity than adults, consistent with the developmental effects. Mean connectivity across entire network regions of interest (ROIs) was also significantly decreased in the ECN in adolescents. However, there were no significant interactions found between age group and user group in any of the seed-based or ROI analyses. There were also no associations found between cannabis use frequency and any of the derived connectivity measures. CONCLUSION: Regular cannabis use is associated with changes in connectivity of the ECN, which may reflect allostatic or compensatory changes in response to regular cannabis intoxication. However, these associations were not significantly different in adolescents compared to adults

Associations between regular cannabis use and brain resting-state functional connectivity in adolescents and adults

Background/aim: Cannabis use is highly prevalent in adolescents; however, little is known about its effects on adolescent brain function. Method: Resting-state functional magnetic resonance imaging was used in matched groups of regular cannabis users (N = 70, 35 adolescents: 16–17 years old, 35 adults: 26–29 years old) and non-regular-using controls (N = 70, 35 adolescents/35 adults). Pre-registered analyses examined the connectivity of seven major cortical and sub-cortical brain networks (default mode network, executive control network (ECN), salience network, hippocampal network and three striatal networks) using seed-based analysis methods with cross-sectional comparisons between user groups and age groups. Results: The regular cannabis use group (across both age groups), relative to controls, showed localised increases in connectivity only in the ECN analysis. All networks showed localised connectivity differences based on age group, with the adolescents generally showing weaker connectivity than adults, consistent with the developmental effects. Mean connectivity across entire network regions of interest (ROIs) was also significantly decreased in the ECN in adolescents. However, there were no significant interactions found between age group and user group in any of the seed-based or ROI analyses. There were also no associations found between cannabis use frequency and any of the derived connectivity measures. Conclusion: Regular cannabis use is associated with changes in connectivity of the ECN, which may reflect allostatic or compensatory changes in response to regular cannabis intoxication. However, these associations were not significantly different in adolescents compared to adults.</p

Regulation of polarised growth in fungi

Polarised growth in fungi occurs through the delivery of secretory vesicles along tracks formed by cytoskeletal elements to specific sites on the cell surface where they dock with a multiprotein structure called the exocyst before fusing with the plasmamembrane. The budding yeast, Saccharomyces cerevisiae has provided a useful model to investigate the mechanisms involved and their control. Cortical markers, provided by bud site selection pathways during budding, the septin ring during cytokinesis or the stimulation of the pheromone response receptors during mating, act through upstream signalling pathways to localise Cdc24, the GEF for the rho family GTPase, Cdc42. Cdc42 in its GTP-bound activates a multiprotein protein complex called the polarisome which nucleates actin cables along which the secretory vesicles are transported to the cell surface. Hyphae can elongate at a rate orders of magnitude faster than the extension of a yeast bud, so understanding hyphal growth will require substantial modification of the yeast paradigm. The rapid rate of hyphal growth is driven by a structure called the Spitzenkörper, located just behind the growing tip and which is rich in secretory vesicles. It is thought that secretory vesicles are delivered to the apical region where they accumulate in the Spitzenkörper. The Spitzenkörper then acts as vesicle supply centre in which vesicles exit the Spitzenkörper in all directions, but because of its proximity, the tip receives a greater concentration of vesicles per unit area than subapical regions. There are no obvious equivalents to the bud site selection pathway to provide a spatial landmark for polarised growth in hyphae. However, an emerging model is the way that the site of polarised growth in the fission yeast, Schizosaccharomyces pombe, is marked by delivery of the kelch repeat protein, Tea1, along microtubules. The relationship of the Spitzenkörper to the polarisome and the mechanisms that promote its formation are key questions that form the focus of current research

Intussusception in Adults: The Role of MDCT in the Identification of the Site and Cause of Obstruction

Unlike pediatric intussusception, intestinal intussusception is infrequent in adults and it is often secondary to a pathological condition. The growing use of Multi-Detector Computed Tomography (MDCT) in abdominal imaging has increased the number of radiological diagnoses of intussusception, even in transient and nonobstructing cases. MDCT is well suited to delineate the presence of the disease and provides valuable information about several features, such as the site of intussusception, the intestinal segments involved, and the extent of the intussuscepted bowel. Moreover, MDCT can demonstrate the complications of intussusceptions, represented by bowel wall ischemia and perforation, which are mandatory to promptly refer for surgery. However, not all intussusceptions need an operative treatment. In this paper, we review the current role of MDCT in the diagnosis and management of intussusception in adults, focusing on features, as the presence of a leading point, that may guide an accurate selection of patients for surgery