Students' use of diagrams for the visualisation of biochemical processes.

Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2003.Research into the usefulness of scientific diagrams as teaching and learning tools has revealed their great effectiveness in reinforcing and replacing text; summarizing, clarifying, grouping and comparing information; illustrating abstract concepts and spatial relations between concepts; and aiding understanding and integration of knowledge. However, these advantages are not always realised as diagram effectiveness depends on the student's cognitive ability, visual literacy and prior knowledge. In biochemistry, flow diagrams are used as tools for the visualisation of biochemical processes, the abstract nature of which presents problems to students, probably because the depicted content is beyond their perceptual experience. In this study, we define visualisation as the entire process from the perception of an external representation (e.g. diagram), its internal processing, and the expression of a mental model of the represented content. Therefore, visualisation incorporates reasoning processes and interactions with a student's conceptual knowledge, in their construction of a mental model. Students' visualisation difficulties, in terms of conceptual and reasoning difficulties, have been well researched in areas such as physics and chemistry, but neglected in biochemistry, especially with respect to the use of diagrams as visualisation tools. Thus the aim of this study was to investigate students' use of diagrams for the visualisation of biochemical processes, and to identify the nature, and potential sources of students' conceptual, reasoning and diagram-related difficulties revealed during the visualisation process. The study groups ranged from 27 to 95 biochemistry students from the University of Natal and 2 to 13 local and international experts. Propositional knowledge was obtained from textbooks and from a questionnaire to experts. Data on student visualisation of biochemical processes was obtained from their responses to written and interview probes as well as student-generated diagrams. All data was subjected to inductive analysis according to McMillan and Schumacher (1993) and any difficulties that emerged were classified at levels 1- 3 on the framework of Grayson et al. (2001). The possible sources of difficulties were considered in terms of a model by Schonborn et al. (2003 & 2002). The results revealed the following major findings. The meaning of linear, cyclic and cascade biochemical processes was partially resolved by means of an extensive list of generic and distinguishing functional features obtained from experts. Attempts to clarify propositional knowledge of the complement system revealed a deficiency in our understanding of the functional relationship between the complement pathways and highlighted the need for further experimental laboratory work. Several students literally interpreted diagrams of the functional characteristics of biochemical processes (e.g. cyclic) as the spatial arrangement of the intermediates within cells (e.g. occur in "circles"), although in some cases, their verbal responses revealed that they did not hold this difficulty suggesting that they might hold more than one internal model of the process. Some students also showed difficulty using textbook diagrams to visualise the chemistry of glycolytic and complement reactions. In this regard, besides students' conceptual knowledge and reasoning ability, a major source of these difficulties included misleading symbolism and visiospatial characteristics in the diagrams, suggesting the need for improvement of diagram design through the use of clearer symbolism, the standardization of conventions, and improvement of visiospatial properties of diagrams. The results constituted further empirical evidence for the model of Schonbom et al. (2003 & 2002) and led to the proposal of a model of visualisation aimed at clarifying the highly complex and cognitive processes involved in individuals' visualisation of biochemical processes in living systems

The advances of technology: A case study of two midwest academic slide libraries

This study focuses on the question of how willing and prepared slide libraries are for technological change. Many slide libraries are at various stages of development, and this fact could either facilitate or inhibit growth when it comes to implementing new technologies: in particular, digitized images in any of the available formats (CD-ROM, laser disc, or online). In this case study of two Midwest academic slide libraries, issues such as funding, size of the library, current technology usage, and knowledge of various technologies, were examined to determine their effects on advancement toward new and innovative systems. These issues were addressed in an in-depth interview process with slide librarians at two different academic institutions. Since digitized images are not yet being used in the slide libraries on which this study is based, interview questions focused on opinions, attitudes and expectations about the implementation of digitization

Sex-Specific Immunization for Sexually Transmitted Infections Such as Human Papillomavirus: Insights from Mathematical Models

Johannes Bogaards and colleagues use mathematical models to investigate whether vaccinating females only, males only, or both sexes is the best way to achieve the most effective reduction in the population prevalence of sexually-transmitted infection

Objectively measured patterns of sedentary time and physical activity in young adults of the Raine study cohort

Background: To provide a detailed description of young adults' sedentary time and physical activity. Methods: 384 young women and 389 young men aged 22.1±0.6 years, all participants in the 22 year old follow-up of the Raine Study pregnancy cohort, wore Actigraph GT3X+ monitors on the hip for 24 h/day over a one-week period for at least one 'valid' day (=10 h of waking wear time). Each minute epoch was classified as sedentary, light, moderate or vigorous intensity using 100 count and Freedson cut-points. Mixed models assessed hourly and daily variation; t-tests assessed gender differences. Results: The average (mean±SD) waking wear time was 15.0±1.6 h/day, of which 61.4±10.1 % was spent sedentary, 34.6±9.1 % in light-, 3.7±5.3 % in moderate- and, 0.3±0.6 % in vigorous-intensity activity. Average time spent in moderate to vigorous activity (MVPA) was 36.2±27.5 min/day. Relative to men, women had higher sedentary time, but also higher vigorous activity time. The 'usual' bout duration of sedentary time was 11.8±4.5 min in women and 11.7±5.2 min in men. By contrast, other activities were accumulated in shorter bout durations. There was large variation by hour of the day and by day of the week in both sedentary time and MVPA. Evenings and Sundays through Wednesdays tended to be particularly sedentary and/or inactive. Conclusion: For these young adults, much of the waking day was spent sedentary and many participants were physically inactive (low levels of MVPA). We provide novel evidence on the time for which activities were performed and on the time periods when young adults were more sedentary and/or less active. With high sedentary time and low MVPA, young adults may be at risk for the life-course sequelae of these behaviours

Australia\u27s health 2002 : the eighth biennial report of the Australian Institute of Health and Welfare

Australia\u27s Health 2002 is the eighth biennial health report of the Australian Institute of Health and Welfare. It is the nation\u27s authoritative source of information on patterns of health and illness, determinants of health, the supply and use of health services, and health service costs and performance. Australia\u27s Health 2002 is an essential reference and information resource for all Australians with an interest in health

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Whole-genome sequencing reveals host factors underlying critical COVID-19

Critical COVID-19 is caused by immune-mediated inflammatory lung injury. Host genetic variation influences the development of illness requiring critical care1 or hospitalization2–4 after infection with SARS-CoV-2. The GenOMICC (Genetics of Mortality in Critical Care) study enables the comparison of genomes from individuals who are critically ill with those of population controls to find underlying disease mechanisms. Here we use whole-genome sequencing in 7,491 critically ill individuals compared with 48,400 controls to discover and replicate 23 independent variants that significantly predispose to critical COVID-19. We identify 16 new independent associations, including variants within genes that are involved in interferon signalling (IL10RB and PLSCR1), leucocyte differentiation (BCL11A) and blood-type antigen secretor status (FUT2). Using transcriptome-wide association and colocalization to infer the effect of gene expression on disease severity, we find evidence that implicates multiple genes—including reduced expression of a membrane flippase (ATP11A), and increased expression of a mucin (MUC1)—in critical disease. Mendelian randomization provides evidence in support of causal roles for myeloid cell adhesion molecules (SELE, ICAM5 and CD209) and the coagulation factor F8, all of which are potentially druggable targets. Our results are broadly consistent with a multi-component model of COVID-19 pathophysiology, in which at least two distinct mechanisms can predispose to life-threatening disease: failure to control viral replication; or an enhanced tendency towards pulmonary inflammation and intravascular coagulation. We show that comparison between cases of critical illness and population controls is highly efficient for the detection of therapeutically relevant mechanisms of disease