Comparative Exoproteome Analysis of Streptococcus suis Human Isolates

The swine pathogen Streptococcus suis is a Gram-positive bacterium which causes infections in pigs, with an impact in animal health and in the livestock industry, and it is also an important zoonotic agent. During the infection process, surface and secreted proteins are essential in the interaction between microorganisms and their hosts. Here, we report a comparative proteomic analysis of the proteins released to the extracellular milieu in six human clinical isolates belonging to the highly prevalent and virulent serotype 2. The total secreted content was precipitated and analyzed by GeLC-MS/MS. In the six strains, 144 proteins assigned to each of the categories of extracellular or surface proteins were identified, as well as 680 predicted cytoplasmic proteins, many of which are putative moonlighting proteins. Of the nine predicted signal peptide-I secreted proteins, seven had relevant antigenic potential when they were analyzed through bioinformatic analysis. This is the first work comparing the exoproteome fraction of several human isolates of this important pathogen

Un nuevo híbrido del género Thymus L. (Lamiaceae).

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Una subespecie nueva en el género Sideritis L. (Lamiaceae)

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Strategies of the Spanish press in the face of the Twitter algorithm change. Analysis of tweets published between 2018-2020

Twitter recently celebrated its 15th anniversary. During this period, the platform has gone through several phases, culminating in a record number of subscribers and profits in 2021. Twitter is a household name all over the world and people know what it can or cannot provide, independent of the future growth that it may experience with new investments and updates. This article aims to verify two interrelated hypotheses, namely: the Spanish press already knows how to optimise the social network Twitter, as three decades have elapsed since its launch; and, secondly, the algorithm modification implemented by Twitter in 2018 has triggered a change in the positioning of the headers studied in this social network. In order to demonstrate both, the object of analysis will be conducted by a mixed approach through quantitative statistical processes (which will study the number of impacts and retweets and likes obtained), and inductive qualitative methods such as semi-structured interviews. This multidisciplinary approach will provide a more complete and in-depth analysis of the phenomenon. The research focuses on the period between 2018 and 2020, and addresses the participation on Twitter of the four main traditional newspapers (El País, La Vanguardia, ABC and El Mundo) as well as four native digital newspapers (20 Minutos, El Español, elDiario.es and El Huffpost). The analysis comprises more than 1.5 million tweets among the eight chosen newspapers.Twitter acaba de celebrar su decimoquinto aniversario. Durante este periodo, la plataforma ha pasado varias fases que han culminado con un récord de suscriptores y beneficios en 2021. El mundo conoce Twitter y sabe qué es lo que puede o no proporcionar, aparte de las posibilidades de crecimiento que pueda experimentar en caso de potenciales inversiones. En el presente artículo se pretende comprobar dos hipótesis interrelacionadas, a saber: la prensa española en castellano ya sabe cómo optimizar la red social Twitter, por haber transcurrido tres lustros desde su puesta en marcha; y, en segundo lugar, el cambio de algoritmo implementado por Twitter en 2018 que ha desencadenado un cambio de posicionamiento de las cabeceras estudiadas en esta red social. Con el propósito de demostrar ambas, se abordará el objeto de análisis desde un enfoque mixto a través de procesos cuantitativos estadísticos (que estudiarán el número de impactos y de retuits y likes obtenidos), y métodos cualitativos inductivos como entrevistas semiestructuradas a los responsables de redes de los medios involucrados. Esta aproximación mixta proporcionará un análisis más completo y profundo sobre el fenómeno. La investigación se centra en el período comprendido entre 2018 y 2020, y aborda la participación en Twitter de cuatro periódicos tradicionales (El País, La Vanguardia, ABC y El Mundo), así como de cuatro periódicos nativos digitales (20 Minutos, El Español, elDiario.es y El Huffpost). El análisis comprende más de 1,5 millones de tuits entre los ocho periódicos elegidos

Microprocessor board for vitro ceramic control

This paper presents the industrial application of a microcontroller for a vitroceramic cooker. The vitroceramic surface is clustered into a matrix with of cells, each one containing one heating resistor and one infrared emitter- receiver pair which detects the presence or absence of a receptacle on its cell. With this information, a microcontroller fires only those resistors arranged under the base of a receptacle. This patented vitroceramic cooker presents many advantages over traditional ones; for instance, power consumption is optimized, as only the useful ceramic surface is heated. Furthermore, when the object is removed from the surface, the heating resistors are powered off, so there is no risk for the fires being left on, which is of great interest for blind people. Acoustic signals can also be generated indicating the existence of heated resistors

Using elephant movements to assess landscape connectivity under Peninsular Malaysia's central forest spine land use policy

One of the most vital and urgent global conservation challenges is to deal with the loss and fragmentation of wildlife habitats, particularly for large‐bodied and wide‐ranging terrestrial megafauna. The Central Forest Spine Master Plan for Ecological Linkages (CFS) was developed by the Malaysian Federal Government in 2010 to protect biodiversity and ecosystem services by securing landscape connectivity between Peninsular Malaysia's main forest blocks. Here we present an evaluation of the effectiveness of the CFS master plan to promote functional connectivity for Asian elephants, one of its focal species. The specific objectives of our study were to identify the most critical forest patches to maintain connectivity for elephants in Peninsular Malaysia, assess functional connectivity within the CFS ecological linkages, and identify alternative corridors where appropriate to enhance CFS effectiveness. We used the largest animal movement dataset in Peninsular Malaysia (220,000 GPS locations from 53 elephants) to develop models of elephant movement probability and to estimate landscape resistance using step selection functions based on landscape characteristics. According to our evaluation of 28 linkages, 57% of them provided high functional connectivity, 28% provided acceptable connectivity, and 14% provided low to no connectivity. A very important and positive finding is that the CFS linkages with the highest centrality values (i.e., the most important to maintain overall connectivity in Peninsular Malaysia) also score highly in functional connectivity (i.e., they are actually effective corridors for elephant movement). This means that an adequate CFS implementation can lead to high levels of functional connectivity among Peninsular Malaysia's main forest blocks. Based on our assessment, we recommend to conduct some revisions on the CFS plan to ensure its effectiveness

LA INVESTIGACIÓN CIENTÍFICA COMO HERRAMIENTA A LA CONSERVACIÓN DEL JAGUAR EN DARIÉN, PANAMÁ

The jaguar is the largest felid in the American continent and is threatened in Panama. Since 1998, we have conducted scientific research to obtain information about the ecology of the jaguar in the Darien National Park and in mixed areas where there are cattle pastures and forest remnants. Based on the information compiled, we learned that 395 jaguars have been killed between 1989 to 2023 in Panama, and Darién is the province where more animals killed were reported. We use camera traps and GPS collars to obtain information about the jaguars, and the information obtained basically is translated for decision making at the level of communities and decision makers. Further, this information is disseminated, good sustainable practices are promoted, and mitigation measures are implemented to promoting the coexistence between the people and jaguars.El jaguar es el felino más grande del continente americano y está en peligro de desaparecer en Panamá. Desde 1998 realizamos investigación científica para obtener información sobre la ecología del jaguar en el Parque Nacional Darién y en zonas mixtas donde hay potreros y remanentes de bosques. Con base en la información recopilada sabemos que 395 jaguares han sido asesinados entre 1989 al 2023 y Darién es la provincia donde se reportan más muertes. Utilizamos cámaras trampas y collares GPS para obtener la información de los jaguares, estos datos son básicamente traducidos en recomendaciones muy puntuales para las comunidades y los tomadores de decisiones. Además, se disemina la información, se promueve la realización de buenas prácticas sostenibles y medidas de mitigación que puedan ayudar a las personas a mejorar la convivencia con los jaguares

Evolución de los flujos de rentas por intereses en España entre 1995 y 1999

Artículo de revistaLas variaciones de los tipos de interes afectan, entre otros, a las rentas que los agentes obtienen y pagan por sus activos financieros y pasivos, pero no es posible establecer a priori si el impacto de esos cambios favorece la transmision de la politica monetaria, pues esto depende, entre otros factores, del saldo financiero inicial de cada agente. En este articulo se ha evaluado este ultimo tipo de efectos y su contribucion a la transmision de dicha politica. (ad