A study of cell membranes in nasal epithelial cells from patients with chronic rhinosinusitis with nasal polyps by means of a fluorescent probe

Aim. To assess the state of membranes in nasal epithelial cells obtained from the patients with chronic rhinosinusitis with nasal polyps (CRSwNP) with the help of the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole. Methods. The state of membrane phospholipid bilayer in suspensions of nasal epithelial cells isolated from ten patients with CRSwNP was evaluated using the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole that reacts on the physico-chemical properties of its microenvironment. Changes in fluorescence spectra were determined using a Thermo Scientific Lumina fluorescence spectrometer (Thermo Fisher Scientific) 1 hour after the addition of the probe to nasal epithelial cell suspensions. Results. CRSwNP was found to be associated with a higher rate of nasal epithelial cell membrane hydration in the region of phospholipid glycerol moiety, carbonyl groups and aliphatic chains of fatty acids attached to the carbonyl groups. Conclusion. Our findings suggest that CRSwNP is accompanied by the elevated hydration rate of the most polar region, namely polar heads of phospholipids of nasal epithelial cell membranes

A study of cell membranes in nasal epithelial cells from patients with chronic rhinosinusitis with nasal polyps by means of a fluorescent probe

Aim. To assess the state of membranes in nasal epithelial cells obtained from the patients with chronic rhinosinusitis with nasal polyps (CRSwNP) with the help of the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole. Methods. The state of membrane phospholipid bilayer in suspensions of nasal epithelial cells isolated from ten patients with CRSwNP was evaluated using the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole that reacts on the physico-chemical properties of its microenvironment. Changes in fluorescence spectra were determined using a Thermo Scientific Lumina fluorescence spectrometer (Thermo Fisher Scientific) 1 hour after the addition of the probe to nasal epithelial cell suspensions. Results. CRSwNP was found to be associated with a higher rate of nasal epithelial cell membrane hydration in the region of phospholipid glycerol moiety, carbonyl groups and aliphatic chains of fatty acids attached to the carbonyl groups. Conclusion. Our findings suggest that CRSwNP is accompanied by the elevated hydration rate of the most polar region, namely polar heads of phospholipids of nasal epithelial cell membranes

Self-assembling DNA-caged particles: nanoblocks for hierarchical self-assembly

DNA is an ideal candidate to organize matter on the nanoscale, primarily due to the specificity and complexity of DNA based interactions. Recent advances in this direction include the self-assembly of colloidal crystals using DNA grafted particles. In this article we theoretically study the self-assembly of DNA-caged particles. These nanoblocks combine DNA grafted particles with more complicated purely DNA based constructs. Geometrically the nanoblock is a sphere (DNA grafted particle) inscribed inside a polyhedron (DNA cage). The faces of the DNA cage are open, and the edges are made from double stranded DNA. The cage vertices are modified DNA junctions. We calculate the equilibriuim yield of self-assembled, tetrahedrally caged particles, and discuss their stability with respect to alternative structures. The experimental feasability of the method is discussed. To conclude we indicate the usefulness of DNA-caged particles as nanoblocks in a hierarchical self-assembly strategy.Comment: v2: 21 pages, 8 figures; revised discussion in Sec. 2, replaced 2 figures, added new reference

Liquid Nonlinear Oscillations in the U-Tube System

Dynamics of oscillation processes in a siphon U-tube is studied for the system of connected vessels filled with homogeneous liquid. The equations and phase paths describing the motion of viscous and non-viscous liquids are given, oscillation frequencies are considered. Oscillations are nonlinear in general case, but they turn into linear by setting specific parameter values of the system. Phase portraits are obtained and their dependences on parameters of the system are analyzedfor both linear and non-linear cases

Zero-energy states in rotating trapped Bose-Einstein condensates

We have calculated low-lying quasiparticle excitation spectra of rotating three-dimensional Bose-Einstein condensates. As opposed to the prediction of hydrodynamic continuum theories, we find a minimum in the Tkachenko mode spectrum at intermediate rotation frequencies of the harmonic trap. Such a minimum can harbour a Tkachenko quasiparticle with zero excitation energy. We discuss the experimental signatures of such a zero mode.Comment: 14 pages, 6 figure

ФРАКЦІЙНИЙ СКЛАД БІЛКІВ У ПАСТАХ ДЛЯ ДИТЯЧОГО ХАРЧУВАННЯ

The paper presents the results of determination of fractional composition of proteins in the pastes for infant food. It was shown that the use of thermostatic production method for technology of protein pastes for infant food and developed starter compositions of monocultures B. animalis Bb-12 and mixed cultures of Lactococcus lactis ssp. with heightened proteolytic properties as part of the frozen bacterial concentrates F DVS C-303 (or F DVS C -303) or of concentrates of FD DVS CH N-11 (or FD-DVS CH N 19 or CH N DVS FD-22 or FD-DVS Flora danica), prepared by freeze-drying, allows to obtain products with hypoallergenic properties. The article specifies that the protein pastes for infant food enriched with lactulose, a complex of vitamins FT 041081EU and a complex of minerals FT 042836EU have the highest hypoallergenic properties: the content of α- and β-casein in the experimental samples of products produced using above mentioned starter compositions is 5,24...5,72 and 4,99...5,21 %, respectively, the content of κ-casein complex+β-lactoglobulin – 1,13...1,42 %.  В статье приведены результаты определения фракционного состава белков в пастах для детского питания. Показано, что использование в технологии белковых паст для детского питания термостатного способа производства и разработанных заквасочных композиций из монокультур B. animalis Bb-12 и смешанных культур Lactococcus lactis ssp. с повышенными протеолитическими свойствами в составе замороженного бакконцентрата F DVS C-303 (или F DVS C-303) или в составе бакконцентрата FD DVS CH N-11 (или FD DVS CH N-19 или FD DVS CH N-22 или FD DVS Flora-danica), полученного лиофильной сушкой, позволяет получить продукты с гипоаллергенными свойствами. Самые высокие гипоаллергенные свойства имеют белковые пасты для детского питания, обогащенные лактулозой, комплексом витаминов FT 041081EU и комплексом минеральных веществ FT 042836EU: содержание α- и β-казеина в экспериментальных образцах продуктов, произведенных с использованием указанных заквасочных композиций, составляет 5,24…5,72 и 4,99...5,21 % соответственно, содержание комплекса κ-казеин+β-лактоглобулин – 1,13...1,42 %.У статті наведено результати визначення фракційного складу білків у пастах для дитячого харчування. Показано, що використання у технології білкових паст для дитячого харчування термостатного способу виробництва і розроблених заквашувальних композицій із монокультур B. animalis Bb-12 та змішаних культур Lactococcus lactis ssp. з підвищеними протеолітичними властивостями у складі замороженого бакконцентрату F DVS C-303 (або F DVS C-303) чи у складі бакконцентрату FD DVS CH N-11 (або FD DVS CH N-19, або FD DVS CH N-22, або FD DVS Flora-danica), отриманого ліофільним сушінням, дозволяє отримати продукти з гіпоалергенними властивостями. Найвищі гіпоалергенні властивості мають білкові пасти для дитячого харчування, збагачені лактулозою, комплексом вітамінів FT 041081EU та комплексом мінеральних речовин FT 042836EU: вміст  α- й β-казеїнів у експериментальних зразках продуктів, вироблених з використанням зазначених заквашувальних композицій, складає 5,24…5,72 й 4,99…5,21 % відповідно, вміст комплексу κ-казеїн+β-лактоглобулін – 1,13…1,42 %. &nbsp

Internal rotation of red giants by asteroseismology

We present an asteroseismic approach to study the dynamics of the stellar interior in red-giant stars by asteroseismic inversion of the splittings induced by the stellar rotation on the oscillation frequencies. We show preliminary results obtained for the red giant KIC4448777 observed by the space mission Kepler.Comment: 3 pages, 4 figures, the 40th Liege International Astrophysical Colloquium Liac40, 'Ageing low mass stars: from red giants to white dwarfs', to be published on EPJ Web of Conference

Парокомпресійні теплонасосні установки в системах теплопостачання

В монографії розглядається підвищення енергоефективності джерел теплопостачання шляхом використання парокомпресійних теплонасосних установок в системах теплопостачання, визначення умов раціонального комбінування джерел теплопостачання і теплонасосних установок в системах теплопостачання, розробка методичних основ синтезу джерел теплопостачання з тепловими насосами. Запропоновано комплексні методи визначення енергоефективності теплонасосних станцій в системах теплопостачання для різних схемних рішень та режимів роботи. Представлені рекомендації можуть бути використані для прогнозування умов ефективної інтеграції теплонасосних установок в системи теплопостачання

Internal rotation of the red-giant star KIC 4448777 by means of asteroseismic inversion

In this paper we study the dynamics of the stellar interior of the early red-giant star KIC 4448777 by asteroseismic inversion of 14 splittings of the dipole mixed modes obtained from {\it Kepler} observations. In order to overcome the complexity of the oscillation pattern typical of red-giant stars, we present a procedure which involves a combination of different methods to extract the rotational splittings from the power spectrum. We find not only that the core rotates faster than the surface, confirming previous inversion results generated for other red giants (Deheuvels et al. 2012,2014), but we also estimate the variation of the angular velocity within the helium core with a spatial resolution of $\Delta r=0.001R$ and verify the hypothesis of a sharp discontinuity in the inner stellar rotation (Deheuvels et al. 2014). The results show that the entire core rotates rigidly with an angular velocity of about $\langle\Omega_c/2\pi\rangle=748\pm18$~nHz and provide evidence for an angular velocity decrease through a region between the helium core and part of the hydrogen burning shell; however we do not succeed to characterize the rotational slope, due to the intrinsic limits of the applied techniques. The angular velocity, from the edge of the core and through the hydrogen burning shell, appears to decrease with increasing distance from the center, reaching an average value in the convective envelope of $\langle\Omega_s/2\pi\rangle=68\pm22$~nHz. Hence, the core in KIC~4448777 is rotating from a minimum of 8 to a maximum of 17 times faster than the envelope. We conclude that a set of data which includes only dipolar modes is sufficient to infer quite accurately the rotation of a red giant not only in the dense core but also, with a lower level of confidence, in part of the radiative region and in the convective envelope.Comment: accepted for publication on Ap

Temperature effect on the temporal dynamic of terrestrial invertebrates in technosols formed after reclamation at a post-mining site in Ukrainian steppe drylands

The research was carried out at the Research Centre of the Dnipro State Agrarian and Economic University in Pokrov city. Sampling was carried out in 2013–2015 on a variant of artificial soil (technosols) formed on loess-like loam, red-brown clay, green-grey clay, technological mixture of rocks, and also formed on loess-like loam with a humus-rich 70 cm top soil layer. To investigate the spatiotemporal variation in the abundance, species richness and species composition of invertebrate assemblages within the experimental polygon, the animals were sampled using pitfall traps. In total, 60 pitfall traps were operated simultaneously during each sampling period. Each year the pitfalls were emptied 26 times every 7–9 days. Invertebrates (Arthropoda and Mollusca) of 6 classes, 13 orders, 50 families and 202 species or parataxonomic units were recorded. Diplopoda was most abundant taxonomic group, though it was represented by only one species Rossiulus kessleri (Lohmander, 1927). Coleoptera and Araneae were the most numerous taxonomic groups. Readily available water for plants, precipitation, wind speed, atmospheric temperature (daily minimum, daily maximum, daily mean), atmospheric humidity and atmospheric pressure were used as environmental predictors. Two dimension geographic coordinates of the sampling locations were used to generate a set of orthogonal eigenvector-based spatial variables. Time series of sampling dates were used to generate a set of orthogonal eigenvector-based temporal variables. The moisture content in the technosols was revealed to be the most important factor determining the temporal dynamics of the terrestrial invertebrate community in conditions of semi-arid climate and the ecosystem which formed as a result of the reclamation process. Following soil moisture, the factor most strongly affecting invertebrates in the technosols was temperature. From the total set of the invertebrates, two relatively homogeneous species groups in terms of thermal preferences were extracted: the microtemperature and mesotemperature groups. The microtemperature species are more tolerant to the thermal factor, and the mesotemperature species are more sensitive. The Huisman-Olff-Fresco approach expanded by Jansen-Oksanen provides a wide set of ecologically relevant models which are able to explain species response. The species response to temperature is affected by a complex of other environmental, temporal and spatial factors. The effect of other factors on the species response must be previously extracted to find real estimations of the species temperature optima and tolerance. The approaches to solving this problem may be the object of future investigation