Toxicity and accumulation of arsenic in wheat (Triticum aestivum L.) varieties of China. fyton 78

Toxicidad y acumulación de arsénico en variedades de trigo (Triticum aestivum L.) en China Abstract. Soil arsenic contamination becomes a potential agricultural and environmental hazard worldwide, and has been a serious problem for safe food production. A field experiment on soil contamination was conducted on four wheat varieties ( Jimai, Gaoyou, Weimai and Wennong) in Eastern China, using 50 or 100 mg arsenic/kg soil. Biomass production and yield components were determined and arsenic concentrations were measured in plant tissues. Differential arsenic effects on wheat varieties were determined at maturity. Results showed that addition of arsenic significantly (p<0.05) reduced root, stem and spike dry weight and yield components, which resulted in the decrease of grain yield per plant. Arsenic concentrations in plant tissues increased significantly (p<0.05) with treatments, and its uptake varied considerably among wheat varieties, plant tissues and arsenic treatments. Arsenic concentrations in plant tissues were as follows: roots > stems > leaves and rachises > grains > glumes > awns. In the arsenic treatments, arsenic concentrations in bran were about 2-3 times higher than those in flour. Most of the arsenic contaminated flour exceeded the Chinese tolerance limit. Arsenic contents of grain parts were dependent on variety and treatment level in polluted soils. Weimai and Wennong showed highest amounts of arsenic in flour than the other varieties at 50 or 100 mg/ kg soil treatment, respectively. Weimai possessed significantly lower (p<0.05) amount of arsenic in bran than any other wheat variety. Results showed significant variety differences in arsenic concentration in polluted areas; it is of outstanding importance that wheat with the lowest possible arsenic concentration is used for food or fodder production. The present results provide scientific basis for revising the standards of wastewater discharges. Key words: Arsenic, wheat (Triticum aestivum L.), biomass, yield components, arsenic toxicity. Resumen. La contaminación del suelo por arsénico es un peligro potencial agrícola y ambiental en el mundo, y ha habido serios problemas para una producción de alimentos saludable. Se condujo un experimento a campo sobre la contaminación del suelo con arsénico en cuatro variedades de trigo ( Jimai, Gaoyou, Weimai, Wennong) en China Oriental usando 50 ó 100 mg de arsénico/kg de suelo. Se determinaron la producción de biomasa y los componentes del rendimiento, y se midió la concentración de arsénico en tejidos vegetales. Los efectos del arsénico sobre las variedades de trigo se determinaron a la madurez del cultivo. Los resultados mostraron que la adición de arsénico redujo significativamente (p<0,05) el peso seco de raíces, tallos y espigas, y los componentes del rendimiento, lo que determinó una disminución en el rendimiento de grano por planta. La concentración de arsénico en los tejidos vegetales se incrementó significativamente (p<0,05) con los tratamientos, y su absorción varió considerablemente entre las variedades de trigo, tejidos vegetales y tratamientos de arsénico. La concentración de arsénico en los tejidos vegetales fue como sigue: raíces > tallos > hojas y raquis > granos > glumas > aristas. En los tratamientos, las concentraciones de arsénico en salvado fueron 2-3 veces más altas que en la harina. La mayor parte de la harina contaminada por arsénico excedió el límite de tolerancia permitido en China. El contenido de arsénico de las partes del grano dependió de la variedad y el tratamiento en las áreas contaminadas. Weimai o Wennong mostraron mayores cantidades de arsénico en la harina que las otras variedades a 50 ó 100 mg de arsénico/kg de suelo, respectivamente. Weimai tuvo una menor (p<0,05) cantidad de arsénico en el salvado que las otras variedades. Los resultados mostraron diferencias significativas entre las variedades en la concentración de arsénico en las áreas contaminadas; es de fundamental importancia que se utilice trigo con la menor concentración posible de arsénico en la producción de alimentos y forraje. Los resultados presentados proveen una base científica para revisar los estándares de la descarga de aguas residuales. Palabras clave: Arsénico, trigo (Triticum aestivum L.), biomasa, componentes del rendimiento, toxicidad con arsénico

A Single Nucleotide Polymorphism in the RASGRF2 Gene Is Associated with Alcoholic Liver Cirrhosis in Men

Background Genetic polymorphisms in the RAS gene family are associated with different diseases, which may include alcohol-related disorders. Previous studies showed an association of the allelic variant rs26907 in RASGRF2 gene with higher alcohol intake. Additionally, the rs61764370 polymorphism in the KRAS gene is located in a binding site for the let-7 micro-RNA family, which is potentially involved in alcohol-induced inflammation. Therefore, this study was designed to explore the association between these two polymorphisms and susceptibility to alcoholism or alcoholic liver disease (ALD). Methods We enrolled 301 male alcoholic patients and 156 healthy male volunteers in this study. Polymorphisms were genotyped by using TaqMan® PCR assays for allelic discrimination. Allelic and genotypic frequencies were compared between the two groups. Logistic regression analysis was performed to analyze the inheritance model. Results The A allele of the RASGRF2 polymorphism (rs26907) was significantly more prevalent among alcoholic patients with cirrhosis (23.2%) compared to alcoholic patients without ALD (14.2%). This difference remained significant in the group of patients with alcohol dependence (28.8% vs. 14.3%) but not in those with alcohol abuse (15.1% vs. 14.4%). Multivariable logistic regression analysis showed that the A allele of this polymorphism (AA or GA genotype) was associated with alcoholic cirrhosis both in the total group of alcoholics (odds ratio [OR]: 2.33, 95% confidence interval [CI]: 1.32–4.11; P = 0.002) and in the group of patients with alcohol dependence (OR: 3.1, 95% CI: 1.50–6.20; P = 0.001). Allelic distributions of the KRAS polymorphism (rs61764370) did not differ between the groups. Conclusions To our knowledge, this genetic association study represents the first to show an association of the RASGRF2 G>A (rs26907) polymorphism with ALD in men, particularly in the subgroup of patients with AD. The findings suggest the potential relevance of the RAS gene family in alcoholism and ALD

Privacy preserving in indoor fingerprint localization and radio map expansion

People spend most of their life time in indoor environments and in all of these environments, Location Service Providers (LSPs) improve users’ navigation. Preserving privacy in Location Based Services (LBSs) is vital for indoor LBSs and fingerprinting based indoor localization method is an emerging technique in indoor localization. In such systems, LSP may be curious and untrusted. Therefore, it is preferred that user estimates its location by using a Partial Radio Map (PRM) which is achieved by LSP, anonymously. In this paper, a privacy preserving method that uses Bloom filter for preserving anonymity and creating PRM during localization process, is proposed. In this method, LSP cannot recognize user identity, which is anonymized by the anonymizer. The proposed method has lower computational complexity compared with methods that use encryption or clustering concepts. The proposed method also has higher accuracy in localization compared with those that use Bloom filter with one random selected AP. Then, in order to decrease the complexity and to increase the accuracy at the same time, we introduce a method that expands the radio map by authenticated users, without compromising their privacy. We also enhance the performance of this method, using Hilbert curve for preserving the ambiguity of users’ location. After verifying the user’s data, LSP sends a certificate to the authenticated users. This certificate can increase the priority of users in LBS requests. Simulation results and measurements show that the proposed method on average improves the localization accuracy up to 16% compared with existing location privacy methods

Observing Kelvin-Helmholtz instability in solar blowout jet

Kelvin–Helmholtz instability (KHI) is a basic physical process in fluids and magnetized plasmas, with applications successfully modelling e.g. exponentially growing instabilities observed at magnetospheric and heliospheric boundaries, in the solar or Earth’s atmosphere and within astrophysical jets. Here, we report the discovery of the KHI in solar blowout jets and analyse the detailed evolution by employing high-resolution data from the Interface Region Imaging Spectrograph (IRIS) satellite launched in 2013. The particular jet we focus on is rooted in the surrounding penumbra of the main negative polarity sunspot of Active Region 12365, where the main body of the jet is a super-penumbral structure. At its maximum, the jet has a length of 90 Mm, a width of 19.7 Mm, and its density is about 40 times higher than its surroundings. During the evolution of the jet, a cavity appears near the base of the jet, and bi-directional flows originated from the top and bottom of the cavity start to develop, indicating that magnetic reconnection takes place around the cavity. Two upward flows pass along the left boundary of the jet successively. Next, KHI develops due to a strong velocity shear (∼204 km s−1) between these two flows, and subsequently the smooth left boundary exhibits a sawtooth pattern, evidencing the onset of the instability

Argot2: a large scale function prediction tool relying on semantic similarity of weighted Gene Ontology terms

Background: Predicting protein function has become increasingly demanding in the era of next generation sequencing technology. The task to assign a curator-reviewed function to every single sequence is impracticable. Bioinformatics tools, easy to use and able to provide automatic and reliable annotations at a genomic scale, are necessary and urgent. In this scenario, the Gene Ontology has provided the means to standardize the annotation classification with a structured vocabulary which can be easily exploited by computational methods.Results: Argot2 is a web-based function prediction tool able to annotate nucleic or protein sequences from small datasets up to entire genomes. It accepts as input a list of sequences in FASTA format, which are processed using BLAST and HMMER searches vs UniProKB and Pfam databases respectively; these sequences are then annotated with GO terms retrieved from the UniProtKB-GOA database and the terms are weighted using the e-values from BLAST and HMMER. The weighted GO terms are processed according to both their semantic similarity relations described by the Gene Ontology and their associated score. The algorithm is based on the original idea developed in a previous tool called Argot. The entire engine has been completely rewritten to improve both accuracy and computational efficiency, thus allowing for the annotation of complete genomes.Conclusions: The revised algorithm has been already employed and successfully tested during in-house genome projects of grape and apple, and has proven to have a high precision and recall in all our benchmark conditions. It has also been successfully compared with Blast2GO, one of the methods most commonly employed for sequence annotation. The server is freely accessible at http://www.medcomp.medicina.unipd.it/Argot2Journal Articleinfo:eu-repo/semantics/publishe

LRP16 Integrates into NF-κB Transcriptional Complex and Is Required for Its Functional Activation

BACKGROUND: Nuclear factor κB (NF-κB)-mediated pathways have been widely implicated in cell survival, development and tumor progression. Although the molecular events of determining NF-κB translocation from cytoplasm to nucleus have been extensively documented, the regulatory mechanisms of NF-κB activity inside the nucleus are still poorly understood. Being a special member of macro domain proteins, LRP16 was previously identified as a coactivator of both estrogen receptor and androgen receptor, and as an interactor of NF-κB coactivator UXT. Here, we investigated the regulatory role of LRP16 on NF-κB activation. METHODOLOGY: GST pull-down and coimmunoprecipitation (CoIP) assays assessed protein-protein interactions. The functional activity of NF-κB was assessed by luciferase assays, changes in expression of its target genes, and its DNA binding ability. Annexin V staining and flow cytometry analysis were used to evaluate cell apoptosis. Immunohistochemical staining of LRP16 and enzyme-linked immunosorbent assay-based evaluation of active NF-κB were performed on primary human gastric carcinoma samples. RESULTS: We demonstrate that LRP16 integrates into NF-κB transcriptional complex through associating with its p65 component. RNA interference knockdown of the endogenous LRP16 in cells leads to impaired NF-κB activity and significantly attenuated NF-κB-dependent gene expression. Mechanistic analysis revealed that knockdown of LRP16 did not affect tumor necrosis factor α (TNF-α)-induced nuclear translocation of NF-κB, but blunted the formation or stabilization of functional NF-κB/p300/CREB-binding protein transcription complex in the nucleus. In addition, knockdown of LRP16 also sensitizes cells to apoptosis induced by TNF-α. Finally, a positive link between LRP16 expression intensity in nuclei of tumor cells and NF-κB activity was preliminarily established in human gastric carcinoma specimens. CONCLUSIONS: Our findings not only indicate that LRP16 is a crucial regulator for NF-κB activation inside the nucleus, but also suggest that LRP16 may be an important contributor to the aberrant activation of NF-κB in tumors

Failure patterns and survival outcomes in triple negative breast cancer (TNBC): a 15 year comparison of 448 non-Hispanic black and white women

Purpose: Triple negative breast cancer (TNBC) is a distinct subtype of breast cancer with unique pathologic, molecular and clinical behavior. It occurs more frequently in young blacks and has been reported to have a shorter disease-free interval. We undertook this study to analyze the demographic characteristics, failure patterns, and survival outcomes in this disease. Methods: A total of 448 non-Hispanic black and white women were identified over a 15 year period from 1996 to 2011. Demographic and clinical information including age, race, menopausal status, stage, tumor characteristics, and treatments were collected. Fisher’s exact test and multivariable Cox regression were used to compare failure patterns and survival outcomes between races. Results: 49 % (n = 223) were black. 59 % patients were between 41 and 60 years, with 18 % ≤40 years. 57 % were premenopausal and 89 % had grade 3 tumors. Stage II (47 %) was most frequent stage at diagnosis followed by stage III (28 %). 32 % had lymphovascular invasion. Adjusting for age, stage, and grade, there was no difference in survival outcomes (OS, DFS, LFFS, and DFFS) between the two races. 62 (14 %) patients failed locally either in ipsilateral breast or chest wall, and 19 (4 %) failed in the regional lymphatics. Lung (18 %) was the most frequent distant failure site with <12 % each failing in brain, liver and bones. Conclusion: Failure patterns and survival outcomes did not differ by race in this large collection of TNBC cases. Lung was the predominate site of distant failure followed by brain, bone, and liver. Few patients failed in the regional lymphatics

Observation of a ppb mass threshoud enhancement in \psi^\prime\to\pi^+\pi^-J/\psi(J/\psi\to\gamma p\bar{p}) decay

The decay channel $\psi^\prime\to\pi^+\pi^-J/\psi(J/\psi\to\gamma p\bar{p})$ is studied using a sample of $1.06\times 10^8$ $\psi^\prime$ events collected by the BESIII experiment at BEPCII. A strong enhancement at threshold is observed in the $p\bar{p}$ invariant mass spectrum. The enhancement can be fit with an $S$-wave Breit-Wigner resonance function with a resulting peak mass of $M=1861^{+6}_{-13} {\rm (stat)}^{+7}_{-26} {\rm (syst)} {\rm MeV/}c^2$ and a narrow width that is $\Gamma<38 {\rm MeV/}c^2$ at the 90% confidence level. These results are consistent with published BESII results. These mass and width values do not match with those of any known meson resonance.Comment: 5 pages, 3 figures, submitted to Chinese Physics

Vesicle miR-195 derived from endothelial cells inhibits expression of serotonin transporter in vessel smooth muscle cells

Serotonin or 5-hydroxytryptamine (5-HT) has been shown to be essential in lots of physiological and pathological processes. It is well known that 5-HT and 5-HT transporter (5-HTT) play important roles in the pulmonary artery in pulmonary hypertension. However, little is known about the function of 5-HTT in other arteries. In this study we found that the expression of 5-HTT was elevated in injured carotid arteries and over-expression of 5-HTT induced proliferation of smooth muscle cells (SMCs); however, this phenotype could be reversed by knocking-down of 5-HTT or endothelial cells conditional medium (EC-CM). A 5-HTT inhibitor, fluoxetine, treated animals also exhibited reduced restenosis after injury. We identified that miR-195 was packaged in the extracellular vesicles from EC-CM. We further confirmed that extracellular vesicles could transfer miR-195 from ECs to SMCs to inhibit the expression of 5-HTT in SMCs and the proliferation of SMCs. These results provide the first evidence that ECs communicate with SMCs via micro-RNA195 in the regulation of the proliferation of SMCs through 5-HTT, which will contribute to a better understanding of communications between ECs and SMCs via micro-RNA. Our findings suggest a potential target for the control of vessel restenosis