Forest investigations by polarimetric AIRSAR data in the Harz mountains

The Harz Mountains in the North of Germany have been a study site for several remote sensing investigations since 1985, as the mountainous area is one of the forest regions in Germany heavily affected by forest decline, especially in the high altitudes above 800 m. In a research program at the University of Berlin, methods are developed for improving remote sensing assessment of forest structure and forest state by additional GIS information, using several datasets for establishing a forest information system. The Harz has been defined as a test site for the SIR-C/X-SAR mission which is going to deliver multifrequency and multipolarizational SAR data from orbit. In a pilot project let by DLR-DFD, these data are to be investigated for forestry and ecology purposes. In preparing a flight campaign to the SIR-C / X-SAR mission, 'MAC EUROPE 1991', performed by NASA/JPL, an area of about 12 km in the Northern Harz was covered with multipolarizational AIRSAR data in the C-, L- and P-band, including the Brocken, the highest mountain of the Harz, with an altitude of 1142 m. The multiparameter AIRSAR data are investigated for their information content on the forest state, regarding the following questions: (1) information on forest stand parameters like forest types, age classes and crown density, especially for the separation of deciduous and coniferous forest; (2) information on the storm damages (since 1972) and the status of regeneration; (3) information on the status of forest destruction because of forest decline; and (4) influence of topography, local incidence angle and soil moisture on the SAR data. Within the project various methods and tools have been developed for the investigation of multipolarimetric radar backscatter responses and for discrimination purposes, in order to use the multipolarization information of the compressed Stokes matrix delivered by JPL

Functional insights from the GC-poor genomes of two aphid parasitoids, Aphidius ervi and Lysiphlebus fabarum.

Parasitoid wasps have fascinating life cycles and play an important role in trophic networks, yet little is known about their genome content and function. Parasitoids that infect aphids are an important group with the potential for biological control. Their success depends on adapting to develop inside aphids and overcoming both host aphid defenses and their protective endosymbionts. We present the de novo genome assemblies, detailed annotation, and comparative analysis of two closely related parasitoid wasps that target pest aphids: Aphidius ervi and Lysiphlebus fabarum (Hymenoptera: Braconidae: Aphidiinae). The genomes are small (139 and 141 Mbp) and the most AT-rich reported thus far for any arthropod (GC content: 25.8 and 23.8%). This nucleotide bias is accompanied by skewed codon usage and is stronger in genes with adult-biased expression. AT-richness may be the consequence of reduced genome size, a near absence of DNA methylation, and energy efficiency. We identify missing desaturase genes, whose absence may underlie mimicry in the cuticular hydrocarbon profile of L. fabarum. We highlight key gene groups including those underlying venom composition, chemosensory perception, and sex determination, as well as potential losses in immune pathway genes. These findings are of fundamental interest for insect evolution and biological control applications. They provide a strong foundation for further functional studies into coevolution between parasitoids and their hosts. Both genomes are available at https://bipaa.genouest.org

Continued Neurogenesis in Adult Drosophila as a Mechanism for Recruiting Environmental Cue-Dependent Variants

Background The skills used by winged insects to explore their environment are strongly dependent upon the integration of neurosensory information comprising visual, acoustic and olfactory signals. The neuronal architecture of the wing contains a vast array of different sensors which might convey information to the brain in order to guide the trajectories during flight. In Drosophila, the wing sensory cells are either chemoreceptors or mechanoreceptors and some of these sensors have as yet unknown functions. The axons of these two functionally distinct types of neurons are entangled, generating a single nerve. This simple and accessible coincidental signaling circuitry in Drosophila constitutes an excellent model system to investigate the developmental variability in relation to natural behavioral polymorphisms. Methodology/Principal Findings A fluorescent marker was generated in neurons at all stages of the Drosophila life cycle using a highly efficient and controlled genetic recombination system that can be induced in dividing precursor cells (MARCM system, flybase web site). It allows fluorescent signals in axons only when the neuroblasts and/or neuronal cell precursors like SOP (sensory organ precursors) undergo division during the precedent steps. We first show that a robust neurogenesis continues in the wing after the adults emerge from the pupae followed by an extensive axonal growth. Arguments are presented to suggest that this wing neurogenesis in the newborn adult flies was influenced by genetic determinants such as the frequency dependent for gene and by environmental cues such as population density. Conclusions We demonstrate that the neuronal architecture in the adult Drosophila wing is unfinished when the flies emerge from their pupae. This unexpected developmental step might be crucial for generating non-heritable variants and phenotypic plasticity. This might therefore constitute an advantage in an unstable ecological system and explain much regarding the ability of Drosophila to robustly adapt to their environment

Forest investigation by polarimetric AIRSAR data in the Harz mountains

The Harz Mountains in the North of Germany have been a study site for several remote sensing investigations since 1985, as the mountainous area is one of the forest regions in Germany heavily affected by forest decline, especially in the high altitudes above 800 m. In a research program at the University of Berlin, methods are developed for improving remote sensing assessment of forest structure and forest state by additional GIS information, using several datasets for establishing a forest information system. The Harz has been defined as a test site for the SIR-C/X-SAR mission which is going to deliver multifrequency and multipolarizational SAR data from orbit. In a pilot project let by DLR-DFD, these data are to be investigated for forestry and ecology purposes. In preparing a flight campaign to the SIR-C / X-SAR mission, 'MAC EUROPE 1991', performed by NASA/JPL, an area of about 12 km in the Northern Harz was covered with multipolarizational AIRSAR data in the C-, L- and P-band, including the Brocken, the highest mountain of the Harz, with an altitude of 1142 m. The multiparameter AIRSAR data are investigated for their information content on the forest state, regarding the following questions: (1) information on forest stand parameters like forest types, age classes and crown density, especially for the separation of deciduous and coniferous forest; (2) information on the storm damages (since 1972) and the status of regeneration; (3) information on the status of forest destruction because of forest decline; and (4) influence of topography, local incidence angle and soil moisture on the SAR data. Within the project various methods and tools have been developed for the investigation of multipolarimetric radar backscatter responses and for discrimination purposes, in order to use the multipolarization information of the compressed Stokes matrix delivered by JPL