Limited susceptibility of mice to Usutu virus (USUV) infection and induction of flavivirus cross-protective immunity

© 2015 Elsevier Inc. Flaviviruses are RNA viruses that constitute a worrisome threat to global human and animal health. In Europe, West Nile virus (WNV) outbreaks have dramatically increased in number and severity in recent years, with dozens of human and horse deaths and a high avian mortality across the continent. Besides WNV, the only clinically relevant mosquito-borne flavivirus detected so far in Europe has been the Usutu virus (USUV), which after being reported for the first time in Austria in 2001, quickly spread across Europe, causing a considerable number of bird deaths and neurological disorders in a few immunocompromised patients. Even though USUV infects multiple avian species that develop antibodies, there is little information about USUV susceptibility, pathogenicity and cross-reactive immunity. Here, the susceptibility of suckling and adult mice to USUV infection and the induction of cross-protective immunity against WNV challenge have been addressed.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), PLATESA (P2013/ABI-2906) from the Comunidad Autónoma de Madrid, and by the Network of Animal Disease Infectiology and Research-European Union (NADIR-EU- 228394)Peer Reviewe

Dirofilariosis and Leishmaniasis in the Northern Region of Serbia

Research in the field of vector borne diseases and zoonozes became a topic of interest in Serbia, during the last decade. Climate changes as well as higher frequency of human and animal movement and travel, especially of dogs, is rising a threat of dirofilariosis and leismaniasis. The presence of native mosquito and sandfly vectors has already been confirmed in the country and some invasive/previously not detected were found. Dirofilariosis and leishmaniasis, which are found more or less often in dogs, cause clinical symptoms which are not obvious and therefore they represent a danger for public health with dogs acting as reservoirs of the infection

The Star Formation History of Galaxies Measured from Individual Pixels. I. The Hubble Deep Field North

We analyze the photometric information contained in individual pixels of galaxies in the Hubble Deep Field North (HDFN) using a new technique, _pixel-z_, that combines predictions of evolutionary synthesis models with photometric redshift template fitting. Each spectral energy distribution template is a result of modeling of the detailed physical processes affecting gas properties and star formation efficiency. The criteria chosen to generate the SED templates is that of sampling a wide range of physical characteristics such as age, star formation rate, obscuration and metallicity. A key feature of our method is the sophisticated use of error analysis to generate error maps that define the reliability of the template fitting on pixel scales and allow for the separation of the interplay among dust, metallicity and star formation histories. This technique offers a number of advantages over traditional integrated color studies. As a first application, we derive the star formation and metallicity histories of galaxies in the HDFN. Our results show that the comoving density of star formation rate, determined from the UV luminosity density of sources in the HDFN, increases monotonically with redshift out to at least redshift of 5. This behavior can plausibly be explained by a smooth increase of the UV luminosity density with redshift coupled with an increase in the number of star forming regions as a function of redshift. We also find that the information contained in individual pixels in a galaxy can be linked to its morphological history. Finally, we derive the metal enrichment rate history of the universe and find it in good agreement with predictions based on the evolving HI content of Lyman-alpha QSO absorption line systems.Comment: Accepted for publication in the Astronomical Journal. Full resolution figures available at http://www.stsci.edu/~aconti/pixelz.htm

Emerging trends in the epidemiology of West Nile and Usutu virus infections in Southern Europe

The epidemiology of West Nile (WNV) and Usutu virus (USUV) has changed dramatically over the past two decades. Since 1999, there have been regular reports of WNV outbreaks and the virus has expanded its area of circulation in many Southern European countries. After emerging in Italy in 1996, USUV has spread to other countries causing mortality in several bird species. In 2009, USUV seroconversion in horses was reported in Italy. Co-circulation of both viruses was detected in humans, horses and birds. The main vector of WNV and USUV in Europe is Culex pipiens, however, both viruses were found in native Culex mosquito species (Cx. modestus, Cx. perexiguus). Experimental competence to transmit the WNV was also proven for native and invasive mosquitoes of Aedes and Culex genera (Ae. albopictus, Ae. detritus, Cx. torrentium). Recently, Ae. albopictus and Ae. japonicus naturally-infected with USUV were reported. While neuroinvasive human WNV infections are well-documented, USUV infections are sporadically detected. However, there is increasing evidence of a role of USUV in human disease. Seroepidemiological studies showed that USUV circulation is more common than WNV in some endemic regions. Recent data showed that WNV strains detected in humans, horses, birds, and mosquitoes mainly belong to lineage 2. In addition to European USUV lineages, some reports indicate the presence of African USUV lineages as well. The trends in WNV/USUV range and vector expansion are likely to continue in future years. This mini-review provides an update on the epidemiology of WNV and USUV infections in Southern Europe within a multidisciplinary "One Health" context

Adaptation of extremely halotolerant black yeast Hortaea werneckii to increased osmolarity: a molecular perspective at a glance

Halophilic adaptations have been studied almost exclusively on prokaryotic microorganisms. Discovery of the black yeast Hortaea werneckii as the dominant fungal species in hypersaline waters enabled the introduction of a new model organism to study the mechanisms of salt tolerance in eukaryotes. Its strategies of cellular osmotic adaptations on the physiological and molecular level revealed novel, intricate mechanisms to combat fluctuating salinity. H. werneckii is an extremely halotolerant eukaryotic microorganism and thus a promising source of transgenes for osmotolerance improvement of industrially important yeasts, as well as in crops

Complete genome sequences of two strains of classical swine fevervirus of subgenotype 2.3 detected during outbreaks in 2005 and 2006in Serbia

AbstractTwo strains of classical swine fever virus (CSFV) (SRB/CSFV/1264/2005 and SRB/CSFV/6168/2006), producing seriousclinical signs of disease during outbreaks in 2005 and 2006 in Serbia, were isolated on porcine kidney cells, and theircomplete genomes were determined by next-generation sequencing. This first complete genome characterization of SerbianCSFV strains provides new data about the evolution of CSFV in the Balkan region and enables further detailed phylogeneticstudies of the various strains

Serological evidence of equine arteritis virus infection and phylogenetic analysis of viral isolates in semen of stallions from Serbia

Abstract Background Equine arteritis virus (EAV) is responsible for infections in equids. It can spread easily within the horse population and has a major impact on the horse breeding industry. No EAV outbreak has ever been reported in Serbia. To determine whether EAV is nonetheless circulating there, especially in the Vojvodina region, 340 horse serum samples were subjected to serology testing to detect EAV antibodies. In parallel, semen samples from three seropositive stallions were collected to evaluate their EAV status, using RT-qPCR and virus isolation on cell culture. Results Horse sera with EAV antibodies represented 15.88% (54/340) of the tested samples, 83.23% (283/340) being negative, and just three samples (0.89%) being uninterpretable due to cytotoxicity. Only 7.2% (10/138) of horses kept by private owners on their own property were seropositive for EAV, whereas 21.8% (44/202) of horses kept on stud farms had EAV antibodies. Phylogenetic analysis showed that the Serbian EAV isolate was most closely related to isolates from the neighbouring Hungary. Conclusions EAV is circulating in the Serbian horse population, especially among the breeding population certainly due to the use of EAV shedder stallions since there is no surveillance programme in Serbia and only limited checks on racehorses. Moreover, phylogenetic analysis indicates that the EAV isolated from a Lipizzaner stallion in Serbia is closely related to isolates from Hungary, and together form a new cluster

Complete Genome Sequence of an Equine Arteritis Virus Strain Isolated from a Lipizzaner Stallion in 2015 in Serbia

International audienceHere, we report the first whole-genome sequence of an equine arteritis virus (EAV) strain, RS1, isolated from the semen of a Lipizzaner stallion held in the Vojvodina region of Serbia

Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019

West Nile virus (WNV) is an arthropod-born pathogen, which is transmitted from wild birds through mosquitoes to humans and animals. At the end of the 20th century, the first West Nile fever (WNF) outbreaks among humans in urban environments in Eastern Europe and the United States were reported. The disease continued to spread to other parts of the continents. In Serbia, the largest number of WNV-infected people was recorded in 2018. This research used spatial statistics to identify clusters of WNV infection in humans and animals in South Banat County, Serbia. The occurrence of WNV infection and risk factors were analyzed using a negative binomial regression model. Our research indicated that climatic factors were the main determinant of WNV distribution and were predictors of endemicity. Precipitation and water levels of rivers had an important influence on mosquito abundance and affected the habitats of wild birds, which are important for maintaining the virus in nature. We found that the maximum temperature of the warmest part of the year and the annual temperature range; and hydrographic variables, e.g., the presence of rivers and water streams were the best environmental predictors of WNF outbreaks in South Banat County

Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by <i>Nosema ceranae</i>

<div><p>Microsporidium <i>Nosema ceranae</i> is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex “BEEWELL AminoPlus” to protect honey bees from immunosuppression induced by <i>N</i>. <i>ceranae</i>. In a laboratory experiment bees were infected with <i>N</i>. <i>ceranae</i> and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p<0.01 or p<0.001) numbers of <i>Nosema</i> spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p<0.01 or p<0.001) in control group on the 12^th day post infection, compared to bees that received the supplement. It was supposed that <i>N</i>. <i>ceranae</i> had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with <i>Nosema</i> infection, which can help us to assume the most suitable period for its application in the hive.</p></div