All is fish that comes to the net: metabarcoding for rapid fisheries catch assessment

Monitoring marine resource exploitation is a key activity in fisheries science and biodiversity conservation. Since research surveys are time‐consuming and costly, fishery‐dependent data (i.e. derived directly from fishing vessels) are increasingly credited with a key role in expanding the reach of ocean monitoring. Fishing vessels may be seen as widely ranging data‐collecting platforms, which could act as a fleet of sentinels for monitoring marine life, in particular exploited stocks. Here, we investigate the possibility of assessing catch composition of single hauls carried out by trawlers by applying DNA metabarcoding to the dense water draining from fishing nets just after the end of hauling operations (hereafter ‘slush’). We assess the performance of this approach in portraying β‐diversity and examining the quantitative relationship between species abundances in the catch and DNA amount in the slush (read counts generated by amplicon sequencing). We demonstrate that the assemblages identified using DNA in the slush satisfactorily mirror those returned by visual inspection of net content (about 71% of species and 86% of families of fish) and detect a strong relationship between read counts and species abundances in the catch. We therefore argue that this approach could be upscaled to serve as a powerful source of information on the structure of demersal assemblages and the impact of fisheries

A rapid and reliable detection procedure of Atlantic trout introgression at the diagnostic lactate dehydrogenase chain-1 gene

The Italian-native Mediterranean brown trout (Salmo ghigii) is a seriously threatened freshwater fish, especially by anthropogenic hybridisation with the domestic strains of Atlantic origin that have been repeatedly released into the wild for angling. A PCR-restriction fragment length polymorphism (RFLP) assay of the diagnostic lactate dehydrogenase chain-1 (LDH-C1) gene sequences has been routinely applied to distinguish exotic from native brown trout lineages and detect Atlantic introgression signals in the Mediterranean wild populations. Here, we used dermal swab DNA obtained from 28 wild trout to improve laboratory procedures to genetically characterise trout samples at the LDH-C1gene through (1) a capillary electrophoresis analysis of the RFLP fragments and (2) the optimisation of a diagnostic single nucleotide polymorphism analysable through mini-sequencing approaches. The developed methods were fully consistent with those obtained through the traditional approach, but their analytical process is almost entirely automated and digitalised, thus improving result readability and accuracy in the detection of alien introgressed traces in wild Mediterranean brown trout populations

PRNP P39L variant is a rare cause of frontotemporal dementia in Iialian population

The missense P39L variant in the prion protein gene (PRNP) has recently been associated with frontotemporal dementia (FTD). Here, we analyzed the presence of the P39L variant in 761 patients with FTD and 719 controls and found a single carrier among patients. The patient was a 67-year-old male, with a positive family history for dementia, who developed apathy, short term memory deficit, and postural instability at 66. Clinical and instrumental workup excluded prion disease. At MRI, bilateral frontal lobe atrophy was present. A diagnosis of FTD was made, with a mainly apathetic phenotype. The PRNP P39L mutation may be an extremely rare cause of FTD (0.13%)

SI113, a Specific Inhibitor of the Sgk1 Kinase Activity that Counteracts Cancer Cell Proliferation

Background/Aims: Published observations on serum and glucocorticoid regulated kinase 1 (Sgk1) knockout murine models and Sgk1-specific RNA silencing in the RKO human colon carcinoma cell line point to this kinase as a central player in colon carcinogenesis and in resistance to taxanes. Methods: By in vitro kinase activity inhibition assays, cell cycle and viability analysis in human cancer model systems, we describe the biologic effects of a recently identified kinase inhibitor, SI113, characterized by a substituted pyrazolo[3,4-d]pyrimidine scaffold, that shows specificity for Sgk1. Results: SI113 was able to inhibit in vitro cell growth in cancer cells derived from tumors with different origins. In RKO cells, this kinase inhibitor blocked insulin-dependent phosphorylation of the Sgk1 substrate Mdm2, the main regulator of p53 protein stability, and induced necrosis and apoptosis when used as a single agent. Finally, SI113 potentiated the effects of paclitaxel on cell viability. Conclusion: Since SI113 appears to be effective in inducing cell death in RKO cells, potentiating paclitaxel sensitivity, we believe that this new molecule could be efficiently employed, alone or in combination with paclitaxel, in colon cancer chemotherapy

Mixed connective tissue disease : state of the art on clinical practice guidelines

© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.Mixed connective tissue disease (MCTD) is a complex overlap disease with features of different autoimmune connective tissue diseases (CTDs) namely systemic sclerosis, poly/dermatomyositis and systemic lupus erythematous in patients with antibodies targeting the U1 small nuclear ribonucleoprotein particle. In this narrative review, we summarise the results of a systematic literature research which was performed as part of the European Reference Network on Rare and Complex Connective Tissue and Musculoskeletal Diseases project, aimed at evaluating existing clinical practice guidelines (CPGs) or recommendations. Since no specific CPGs on MCTD were found, other CPGs developed for other CTDs were taken into consideration in order to discuss what can be applied to MCTD even if designed for other diseases. Three major objectives were proposed for the future development of CPGs: MCTD diagnosis (diagnostic criteria), MCTD initial and follow-up evaluations, MCTD treatment. Early diagnosis, epidemiological data, assessment of burden of disease and QOL aspects are among the unmet needs identified by patients.This publication was funded by the European Union’s Health Programme (2014-2020)info:eu-repo/semantics/publishedVersio

The Silent Epidemic of Diabetic Ketoacidosis at Diagnosis of Type 1 Diabetes in Children and Adolescents in Italy During the COVID-19 Pandemic in 2020

To compare the frequency of diabetic ketoacidosis (DKA) at diagnosis of type 1 diabetes in Italy during the COVID-19 pandemic in 2020 with the frequency of DKA during 2017-2019

Postfordismo e trasformazione urbana.Casi di recupero dei vuoti industriali e indicazioni per le politiche nel territorio torinese

Il volume sulle aree dismesse e la trasformazione urbana promosso dalla Direzione Competitività della Regione Piemonte e dall'ente strumentale di ricerca economica e sociale, IRES Piemonte, racchiude contributi su esperienze di recupero dei vuoti urbani industriali e casi rilevanti della trasformazione urbana che hanno interessato Torino e la sua area metropolitana.- Prefazione #7- Introduzione #9- 1. Le ristrutturazioni industriali e il territorio: crisi, declino,metamorfosi? #33- 2. Scoprire i vuoti industriali: analisi e riflessioni a partire da censimenti e mappature di aree industriali dismesse a Torino #51- 3. Le aree dismesse nella riqualificazione e nella rigenerazione urbana a Torino (1990-2015) #113- 4. La trasformazione urbana tra grandi interventi e architetture “ordinarie”. #147- 5. Due esperienze di rigenerazione urbana sul territorio torinese:il Comitato Parco Dora sulla Spina 3 e il Comitato Urban in Barriera di Milano, a Torino #169- 6. Da cittadella industriale a Spina 3: una riconversione incompiuta #193- 7. Ex Diatto - Ex Westinghouse, due casi emblematici per le politiche di rivitalizzazione delle aree industriali torinesi #211- 8. Mirafiori. Dalla componentistica allo yogurt: storia di un progetto di re-industrializzazione #229- 9. Area OSI OVEST-NORD: Toolbox Coworking! #247- 10. Abilitare il territorio metropolitano alla rigenerazione e ad un nuovo sviluppo. Il caso di None, dall'industria subita all'industria inseguita #275- 11. Le ex-Acciaierie Mandelli di Collegno: la storia di una fabbrica,l'attualità del dibattito urbanistico. #301- 12. I programmi territoriali quale motore di rigenerazione urbana e di politiche di contenimento del consumo di suolo nel comune di Settimo Torinese #323- 13. La Regione Piemonte e la sfida del contenimento del consumo di suolo e del riutilizzo delle aree dismesse #353- 14. Torino, la nascita della città postindustriale: quale bilancio? #365- 15. Valutare i rischi della riqualificazione urbanistica e ambientale delle aree industriali dismesse #381- 16. Le aree industriali dismesse e il loro impatto sulla salute: il ruolo dei cittadini e delle amministrazioni locali nell'identificazione dei problemi e delle possibili soluzioni. #405- 17. I giovani e la città che cambia. Nuovi passi e nuovi sguardi sulle tracce di un passato industriale #427- Elenco degli autori e delle autrici #44

Data from: MHC structuring and divergent allele advantage in a urodele amphibian: a hierarchical multi-scale approach

The proteins encoded by extraordinarily polymorphic major histocompatibility complex (MHC) genes are involved in the adaptive immune response. Balancing selection is believed to maintain MHC polymorphism in the long-term, although neutral processes also play a role in shaping MHC diversity. However, the relative contribution of these processes is poorly understood. Here we characterized MHC class II variation of a low-dispersal, pond-breeding newt (Triturus carnifex) over a restricted, geographically structured area. We aimed to: (1) evaluate the contribution of selection and neutral processes to shaping MHC diversity at two geographic scales; and (2) test for signatures of divergent allele advantage (DAA), a potentially important mechanism of balancing selection. The dominant role of selection in shaping MHC variation was suggested by the lack of correlation between MHC and neutral (microsatellite) variation. Although most variation occurred within populations for both types of markers, they differed in the extent of structuring at the two spatial scales. MHC structuring was more pronounced at local scales, suggesting the role of local selection, while structuring was not detectable at a larger scale, possibly an effect of balancing selection. Microsatellites showed the opposite pattern. As expected under DAA, the observed genotypes combined more sequence diversity than expected under random association of alleles. Thus, DAA may contribute to maintaining MHC polymorphism, which is ancient, as supported by signatures of historical positive selection and trans-species polymorphism. Our results point to the importance of a multi-scale approach in studying MHC variation, especially in low-dispersal taxa, genetically structured at fine spatial scales