Resting respiratory tract dendritic cells preferentially stimulate T helper cell Type 2(Th2) responses and require obligatory cytokine signals for induction of Th1 immunity

Consistent with their role in host defense, mature dendritic cells (DCs) from central lymphoid organs preferentially prime for T helper cell type 1 (Th1)-polarized immunity. However, the “default” T helper response at mucosal surfaces demonstrates Th2 polarity, which is reflected in the cytokine profiles of activated T cells from mucosal lymph nodes. This study on rat respiratory tract DCs (RTDCs) provides an explanation for this paradox. We demonstrate that freshly isolated RTDCs are functionally immature as defined in vitro, being surface major histocompatibility complex (MHC) II lo, endocytosishi, and mixed lymphocyte reactionlo, and these cells produce mRNA encoding interleukin (IL)-10. After ovalbumin (OVA)-pulsing and adoptive transfer, freshly isolated RTDCs preferentially stimulated Th2-dependent OVA-specific immunoglobulin (Ig)G1 responses, and antigen-stimulated splenocytes from recipient animals produced IL-4 in vitro. However, preculture with granulocyte/macrophage colony stimulating factor increased their in vivo IgG priming capacity by 2–3 logs, inducing production of both Th1- and Th2-dependent IgG subclasses and high levels of IFN-γ by antigen-stimulated splenocytes. Associated phenotypic changes included upregulation of surface MHC II and B7 expression and IL-12 p35 mRNA, and downregulation of endocytosis, MHC II processing– associated genes, and IL-10 mRNA expression. Full expression of IL-12 p40 required additional signals, such as tumor necrosis factor α or CD40 ligand. These results suggest that the observed Th2 polarity of the resting mucosal immune system may be an inherent property of the resident DC population, and furthermore that mobilization of Th1 immunity relies absolutely on the provision of appropriate microenvironmental costimuli

Estudio técnico-económico de una planta desaladora de agua de mar por ósmosis inversa

En el següent escrit: Estudi técnic-económic d’una planta desaladora d’aigua de mar per osmosis inversa, es pretén donar al lector una idea del procés d’Osmosis Inversa; el qual, ja el podríem ubicar dins dels processos de separació per membranes. Es pretén que aquesta idea sigui el més pràctica possible, sense deixar de banda els aspectes teòrics corresponents, però descartant aquells que, encara tenir relativa importància, l’únic que se aconseguiria amb el seu estudi, seria ampliar el contingut de l’anomenat projecte sense aportarnos això cap benefici relacionat amb l’essència del tema, com es la desalació d’aigua de mar per osmosis inversa. Es per aquest motiu que l’estudi que segueix, es divideix en cinc volums. El primer recull tots els aspectes teórico-práctics, on el lector pot trobar els diferents processos de separació per membranes, les consideracions sobre l’aigua i tot el relacionat amb el fonament fisico-químic que ens pertoca, o sia, la ósmosis inversa: definicions, conceptes bàsics, procés, membranes, etc. L’aspecte pràctic al que ens referim ve reflexat en petits sub-apartats, tals com: neteja eficaç de les membranes, o en seccions posteriors, en petits apartats on es donen recomanacions pràctiques, de instal•lació i inclòs de fabricació d’aparells per postratament. El volum segon és una descripció detalla de l’instal•lació, on s’estudia des de la presa d’aigua de mar fins l’obtenció d’aigua producte, nomenant-se tots els equips, vàlvules, bombes, filtres, etc., que l’aigua travessa en el seu procés. Els tres volums restants corresponen al aspecte més tècnic, on es mostren, por ordre: càlculs, plànols i pressupost o memòria econòmica

Pions in isospin asymmetric matter and nuclear Drell-Yan scattering

Using a self-consistent delta-hole model the pion propagation in isospin asymmetric nuclear matter is studied. In neutron-rich matter, corresponding to heavy nuclei, a significant difference in positive and negative pion light-cone distributions is obtained leading to a nuclear enhancement of up antiquark distribution compared to the down antiquark one. This means that the sea-quark asymmetry in the free nucleon cannot be extracted model independently from an experiment on a neutron-rich nucleus.Comment: 10 pages, 3 figures (included), Latex 2.09, uses elsart.sty, epsf.st

Effect of short-range electron correlations in dynamic transport in a Luttinger liquid

The density operator in the Luttinger model consists of two components, one of which describes long-wave fluctuations and the other is related to the rapid oscillations of the charge-density-wave (CDW) type, caused by short-range electron correlations. It is commonly believed that the conductance is determined by the long-wave component. The CDW component is considered only when an impurity is present. We investigate the contribution of this component to the dynamic density response of a Luttinger liquid free from impurities. We show that the conventional form of the CDW density operator does not conserve the number of particles in the system. We propose the corrected CDW density operator devoid of this shortcoming and calculate the dissipative conductance in the case when the one-dimensional conductor is locally disturbed by a conducting probe. The contribution of the CDW component to conductance is found to dominate over that of the long-wave component in the low-frequency regime.Comment: 6 pages, 4 figures; updated to the published versio

Calcite–magnesite solid solutions : using genetic algorithms to understand non-ideality

We show how a genetic algorithm (GA) generates efficiently the energy landscape of the equimolar calcite–magnesite (CaCO3—MgCO3) solid solution. Starting from a random configuration of cations and a supercell containing 480 atoms, the lowest energy form of ordered dolomite was found in all runs, in 94% of which it was located with less than 20,000 fitness evaluations. Practical implementation and operation of the GA are discussed in detail. The method can also generate both low-lying and high-lying excited states. Detailed analysis of the energy-minimised structures of the different configurations reveals that low energies are associated with reduction of strain associated with rotation of the carbonate groups, a mechanism possible only when a carbonate layer lies between a layer of just Ca and a layer of just Mg. Such strain relief is not possible in the equimolar MgO–CaO solid solution despite the similarity of the crystal structures of these binary oxides to calcite–magnesite, and therefore, the enthalpy of mixing is very high. Implications for thermodynamic configurational averaging over the minima in the energy landscape are briefly considered. Overall, the genetic algorithm is shown to be a powerful tool in probing non-ideality in solid solutions and revealing the ordering patterns that give rise to such behaviour

Switch from sexual to parthenogenetic reproduction in a zebra shark

Parthenogenesis is a natural form of asexual reproduction in which embryos develop in the absence of fertilisation. Most commonly found in plants and invertebrate organisms, an increasing number of vertebrate species have recently been reported employing this reproductive strategy. Here we use DNA genotyping to report the first demonstration of an intra-individual switch from sexual to parthenogenetic reproduction in a shark species, the zebra shark Stegostoma fasciatum. A co-housed, sexually produced daughter zebra shark also commenced parthenogenetic reproduction at the onset of maturity without any prior mating. The demonstration of parthenogenesis in these two conspecific individuals with different sexual histories provides further support that elasmobranch fishes may flexibly adapt their reproductive strategy to environmental circumstances

Epitope recognition of peptide-imprinted polymers for Regenerating protein 1 (REG1)

Molecularly imprinted polymers (MIPs) were developed to replace natural antibodies with a cost-effective and durable synthetic material. Molecular imprinting of proteins conventionally utilizes the whole protein as the template, which is complex (as many different epitopes may be imprinted) and expensive. In this work, seven peptides (13–18 amino acids) were synthesized and used as templates for the imprinting and recognition of Regenerating Protein 1 (REG1). REG1 is involved in the proliferation and differentiation of diverse cell types, and was recently described as a urinary biomarker for pancreatic ductal adenocarcinoma (PDAC). Peptide-imprinted poly(ethylene-co-vinyl alcohol)s (PIPs), containing four different mole fractions of ethylene were cast on screen-printed electrodes to find the optimum composition for both the sensing and the extraction of REG1 in an E. coli culture medium. Peptides with fewer than 16 amino acids and two or three aromatic and hydrophobic groups have a higher affinity for MIPs of poly(ethylene-co-vinyl alcohol) (EVAL) with 27 mol% of ethylene, while those with four aromatic and hydrophobic groups have a higher affinity for MIPs with EVALs that contain 32 mol% of ethylene. The peptide / EVAL combination that maximized both imprinting effectiveness and response to REG1B was the sequence NEDRETWVDADLY imprinted into 32 mol% EVAL. This EVAL composition and template peptide were then modified by incorporation of magnetic nanoparticles, thus extending applications for PIPs to include extraction of REG1 protein from E. coli culture medium

Coulomb blockade of strongly coupled quantum dots studied via bosonization of a channel with a finite barrier

A pair of quantum dots, coupled through a point contact, can exhibit Coulomb blockade effects that reflect an oscillatory term in the dots' total energy whose value depends on whether the total number of electrons on the dots is even or odd. The effective energy associated with this even-odd alternation is reduced, relative to the bare Coulomb blockade energy for uncoupled dots, by a factor (1-f) that decreases as the interdot coupling is increased. When the transmission coefficient for interdot electronic motion is independent of energy and the same for all channels within the point contact (which are assumed uncoupled), the factor (1-f) takes on a universal value determined solely by the number of channels and the dimensionless conductance g of each individual channel. This paper studies corrections to the universal value of (1-f) that result when the transmission coefficent varies over energy scales of the size of the bare Coulomb blockade energy. We consider a model in which the point contact is described by a single orbital channel containing a parabolic barrier potential, and we calculate the leading correction to (1-f) for one-channel (spin-split) and two-channel (spin-degenerate) point contacts in the limit where the single orbital channel is almost completely open. By generalizing a previously used bosonization technique, we find that, for a given value of the dimensionless conductance g, the value of (1-f) is increased relative to its value for a zero-thickness barrier, but the absolute value of the increase is small in the region where our calculations apply.Comment: 13 pages, 3 Postscript figure

Spliced DNA sequences in the Paramecium germline: their properties and evolutionary potential

Despite playing a crucial role in germline-soma differentiation, the evolutionary significance of developmentally regulated genome rearrangements (DRGRs) has received scant attention. An example of DRGR is DNA splicing, a process that removes segments of DNA interrupting genic and/or intergenic sequences. Perhaps, best known for shaping immune-system genes in vertebrates, DNA splicing plays a central role in the life of ciliated protozoa, where thousands of germline DNA segments are eliminated after sexual reproduction to regenerate a functional somatic genome. Here, we identify and chronicle the properties of 5,286 sequences that putatively undergo DNA splicing (i.e., internal eliminated sequences [IESs]) across the genomes of three closely related species of the ciliate Paramecium (P. tetraurelia, P. biaurelia, and P. sexaurelia). The study reveals that these putative IESs share several physical characteristics. Although our results are consistent with excision events being largely conserved between species, episodes of differential IES retention/excision occur, may have a recent origin, and frequently involve coding regions. Our findings indicate interconversion between somatic—often coding—DNA sequences and noncoding IESs, and provide insights into the role of DNA splicing in creating potentially functional genetic innovation

The Hyperon-Nucleon Interaction Potential in the Bound State Soliton Model: The ΛN\Lambda N Case

We develop the formalism to study the hyperon-nucleon interaction potential within the bound state approach to the SU(3) Skyrme model. The general framework is illustrated by applying it to the diagonal $\Lambda N$ potential. The central, spin-spin and tensor components of this interaction are obtained and compared with those derived using alternative schemes.Comment: source file, 4 ps figure