An acceptor-substrate binding site determining glycosyl transfer emerges from mutant analysis of a plant vacuolar invertase and a fructosyltransferase

Glycoside hydrolase family 32 (GH32) harbors hydrolyzing and transglycosylating enzymes that are highly homologous in their primary structure. Eight amino acids dispersed along the sequence correlated with either hydrolase or glycosyltransferase activity. These were mutated in onion vacuolar invertase (acINV) according to the residue in festuca sucrose:sucrose 1-fructosyltransferase (saSST) and vice versa. acINV(W440Y) doubles transferase capacity. Reciprocally, saSST(C223N) and saSST(F362Y) double hydrolysis. SaSST(N425S) shows a hydrolyzing activity three to four times its transferase activity. Interestingly, modeling acINV and saSST according to the 3D structure of crystallized GH32 enzymes indicates that mutations saSST(N425S), acINV(W440Y), and the previously reported acINV(W161Y) reside very close together at the surface in the entrance of the active-site pocket. Residues in- and outside the sucrose-binding box determine hydrolase and transferase capabilities of GH32 enzymes. Modeling suggests that residues dispersed along the sequence identify a location for acceptor-substrate binding in the 3D structure of fructosyltransferases

Cross-talk between signaling pathways leading to defense against pathogens and insects

In nature, plants interact with a wide range of organisms, some of which are harmful (e.g. pathogens, herbivorous insects), while others are beneficial (e.g. growth-promoting rhizobacteria, mycorrhizal fungi, and predatory enemies of herbivores). During the evolutionary arms race between plants and their attackers, primary and secondary immune responses evolved to recognize common or highly specialized features of microbial pathogens (Chisholm et al., 2006), resulting in sophisticated mechanisms of defense

Invloed van verschillende landinrichtingsscenario's op de bodem- en waterafvoer in het zuidelijke deel van de ruilverkaveling Groesbeek

Dit onderzoek heeft tot doel aan te geven hoe het zuidelijke hellinggebied van de ruilverkaveling Groesbeek kan worden ingericht om water- en bodemafstroming zoveel mogelijk te beperken. Er is gerekend met het bodem- en waterafvoermodel LISEM voor vijf landinrichtingsscenario's. Bij drie van de vier knelpunten (Klein America, St. Jansberg, Grafwegen) is de overlast gedeeltelijk terug te dringen door invoering van een van de voorgestelde scenario's. Bij het knelpunt kassen is het landgebruik al dusdanig (gras) dat voorgestelde scenario's geen effect laten zien. Om de overlast volledig terug te dringen zijn voor alle knelpunten aanvullende maatregelen nodig in de vorm van aanleg van retentiebekkens en/of afvoersloten, of de introductie van aanvullendebeheersmaatregelen

Rhizobium nodulation protein NodA is a host specific determinant of the transfer of fatty acids in Nod factor biosynthesis

Microbial Biotechnolog

De Ruimtescanner in Ruimte voor Landbouw; data- en modelaanpassingen

Dit rapport is bedoeld als technisch achtergronddocument bij de studie 'Ruimte voor Landbouw'. Die studie is in samenwerking uitgevoerd door het Ruimtelijke Planbureau (RPB), het Milieu- en Natuurplanbureau (MNP), het LEI en de VU, en betreft de toepassing van de Ruimtescanner om de toekomstige ruimtelijke ontwikkeling van de landbouw te verkennen

Are Small GTPases Signal Hubs in Sugar-Mediated Induction of Fructan Biosynthesis?

External sugar initiates biosynthesis of the reserve carbohydrate fructan, but the molecular processes mediating this response remain obscure. Previously it was shown that a phosphatase and a general kinase inhibitor hamper fructan accumulation. We use various phosphorylation inhibitors both in barley and in Arabidopsis and show that the expression of fructan biosynthetic genes is dependent on PP2A and different kinases such as Tyr-kinases and PI3-kinases. To further characterize the phosphorylation events involved, comprehensive analysis of kinase activities in the cell was performed using a PepChip, an array of >1000 kinase consensus substrate peptide substrates spotted on a chip. Comparison of kinase activities in sugar-stimulated and mock(sorbitol)-treated Arabidopsis demonstrates the altered phosphorylation of many consensus substrates and documents the differences in plant kinase activity upon sucrose feeding. The different phosphorylation profiles obtained are consistent with sugar-mediated alterations in Tyr phosphorylation, cell cycling, and phosphoinositide signaling, and indicate cytoskeletal rearrangements. The results lead us to infer a central role for small GTPases in sugar signaling

Geophysical and geochemical constraints on geoneutrino fluxes from Earth's mantle

Knowledge of the amount and distribution of radiogenic heating in the mantle is crucial for understanding the dynamics of the Earth, including its thermal evolution, the style and planform of mantle convection, and the energetics of the core. Although the flux of heat from the surface of the planet is robustly estimated, the contributions of radiogenic heating and secular cooling remain poorly defined. Constraining the amount of heat-producing elements in the Earth will provide clues to understanding nebula condensation and planetary formation processes in early Solar System. Mantle radioactivity supplies power for mantle convection and plate tectonics, but estimates of mantle radiogenic heat production vary by a factor of more than 20. Recent experimental results demonstrate the potential for direct assessment of mantle radioactivity through observations of geoneutrinos, which are emitted by naturally occurring radionuclides. Predictions of the geoneutrino signal from the mantle exist for several established estimates of mantle composition. Here we present novel analyses, illustrating surface variations of the mantle geoneutrino signal for models of the deep mantle structure, including those based on seismic tomography. These variations have measurable differences for some models, allowing new and meaningful constraints on the dynamics of the planet. An ocean based geoneutrino detector deployed at several strategic locations will be able to discriminate between competing compositional models of the bulk silicate Earth.Comment: 34 pages, 6 tables, 5 figures, 2 supplementary figures; revised version submitted to Earth Planet. Sci. Let

Role of rhizobial lipo-oligosacharides in root nodule formation on leguminious plants

Microbial Biotechnolog

Development of anisotropic structure in the Earth's lower mantle by solid-state convection

Seismological observations reveal highly anisotropic patches at the bottom of the Earth's lower mantle, whereas the bulk of the mantle has been observed to be largely isotropic(1-4). These patches have been interpreted to correspond to areas where subduction has taken place in the past or to areas where mantle plumes are upwelling, but the underlying cause for the anisotropy is unknown-both shape-preferred orientation of elastically heterogenous materials(5) and lattice-preferred orientation of a homogeneous material(6-8) have been proposed. Both of these mechanisms imply that large-strain deformation occurs within the anisotropic regions, but the geodynamic implications of the mechanisms differ. Shape-preferred orientation would imply the presence of large elastic (and hence chemical) heterogeneity whereas lattice-preferred orientation requires deformation at high stresses. Here we show, on the basis of numerical modelling incorporating mineral physics of elasticity and development of lattice-preferred orientation, that slab deformation in the deep lower mantle can account for the presence of strong anisotropy in the circum-Pacific region. In this model-where development of the mineral fabric (the alignment of mineral grains) is caused solely by solid-state deformation of chemically homogeneous mantle material-anisotropy is caused by large-strain deformation at high stresses, due to the collision of subducted slabs with the core-mantle boundary.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62804/1/416310a.pd

Kinome Profiling Reveals an Interaction Between Jasmonate, Salicylate and Light Control of Hyponastic Petiole Growth in Arabidopsis thaliana

Plants defend themselves against infection by biotic attackers by producing distinct phytohormones. Especially jasmonic acid (JA) and salicylic acid (SA) are well known defense-inducing hormones. Here, the effects of MeJA and SA on the Arabidopsis thaliana kinome were monitored using PepChip arrays containing kinase substrate peptides to analyze posttranslational interactions in MeJA and SA signaling pathways and to test if kinome profiling can provide leads to predict posttranslational events in plant signaling. MeJA and SA mediate differential phosphorylation of substrates for many kinase families. Also some plant specific substrates were differentially phosphorylated, including peptides derived from Phytochrome A, and Photosystem II D protein. This indicates that MeJA and SA mediate cross-talk between defense signaling and light responses. We tested the predicted effects of MeJA and SA using light-mediated upward leaf movement (differential petiole growth also called hyponastic growth). We found that MeJA, infestation by the JA-inducing insect herbivore Pieris rapae, and SA suppressed low light-induced hyponastic growth. MeJA and SA acted in a synergistic fashion via two (partially) divergent signaling routes. This work demonstrates that kinome profiling using PepChip arrays can be a valuable complementary ∼omics tool to give directions towards predicting behavior of organisms after a given stimulus and can be used to obtain leads for physiological relevant phenomena in planta