User documentation for the MSK and OMS intelligent tutoring systems

This user's guide describes how to use the Intelligent Tutoring Systems for the Manual Select Keyboard (MSK) and the Orbital Maneuvering System (OMS) and how to use the C code that runs the mockup version of the MSK

Formation of Actin Networks in Microfluidic Concentration Gradients

The physical properties of cytoskeletal networks are contributors in a number of mechanical responses of cells, including cellular deformation and locomotion, and are crucial for the proper action of living cells. Local chemical gradients modulate cytoskeletal functionality including the interactions of the cytoskeleton with other cellular components. Actin is a major constituent of the cytoskeleton. Introducing a microfluidic-based platform, we explored the impact of concentration gradients on the formation and structural properties of actin networks. Microfluidic-controlled flow-free and steady-state experimental conditions allow for the generation of chemical gradients of different profiles, such as linear or step-like. We discovered specific features of actin networks emerging in defined gradients. In particular, we analyzed the effects of spatial conditions on network properties, bending rigidities of network links, and the network elasticity

Response of silicon solar cell to pulsed laser illumination

The response of silicon solar cell(s) to pulsed laser illumination is discussed. The motivation was due to the interest of Earth to space/Moon power beaming applications. When this work began, it was not known if solar cells would respond to laser light with pulse lengths in the nanosecond range and a repetition frequency in the kHz range. This is because the laser pulse would be shorter than the minority carrier lifetime of silicon. A 20-nanosecond (ns) full width half max (FWHM) pulse from an aluminum-gallium/arsenide (Al-Ga-As) diode laser was used to illuminate silicon solar cells at a wavelength of 885 nanometers (nm). Using a high-speed digital oscilloscope, the response of the solar cells to individual pulses across various resistive loads was observed and recorded

Larger, Lighter Space Telescopes by Implementing In-Space Manufacturing Concepts

There is a continuous demand for larger, lighter, and higher quality telescopes from both the astronomical and global surveillance communities one looking up and the other down. Enabling technologies must be developed and implemented that will allow this goal to be financially and technically feasible. The optical systems needed far high spatial resolution surveillance and astronomical applications require large optical, apertures with mention of future systems up to 150 meter in diameter. With traditional optical manufacturing technologies, large optical aperture means high mass and long fabrication lead times with associated high costs. Completely new approaches to optical fabrication must be developed to enable the fabrication of such optical systems. The cost and lead time associated with the fabrication of lightweight, high quality optical systems limits the feasible size of the optics. A primary factor in the launch cost of space optical systems is volume and mass. To minimize the mass of the high quality optics, optical fabricators implement materials with high specific stiffness and use honeycomb, or other structural minimization patterns, to support the optical surface; however, the structure must still be designed to survive launch loads. This sigmficantly adds to the fabrication difficulty and dramatically increases launch costs. One approach to minimizing launch volume and negating the need for the design to survive launch loads is to send the manufacturing facility and raw materials into space and perform the fabrication in-situ. We, are currently performing feasibility studies of initial concepts for inspace manufacturing of optical systems. By utilizing the micro-gravity and vacuum environment of space while eliminating the constraints defined by high launch forces and limited volume of the launch vehicle, the development of large, high quality glass membrane mirrors may be feasible. Several concepts were investigated to address the manufacturing of both optical surfaces and telescope structure. We will describe one of the primary approaches to utilize the space environment for optical manufacturing and describe initial results

Vacuum Strength of Two Candidate Glasses for a Space Observatory

The strengths of two candidate glass types for use in a space observatory were measured. Samples of ultra-low expansion glass (ULE) and borosilicate (Pyrex) were tested in air and in vacuum at room temperature (20 C) and in vacuum after being heated to 200 C. Both glasses tested in vacuum showed an increase in strength over those tested in air. However, there was no statistical difference between the strength of samples tested in vacuum at room temperature and those tested in vacuum after heating to 200 C

Identification of Spiroplasma insolitum symbionts in Anopheles gambiae

Background: Insect symbionts have the potential to block the transmission of vector-borne diseases by their hosts. The advancement of a symbiont-based transmission blocking strategy for malaria requires the identification and study of Anopheles symbionts. Methods: High throughput 16S amplicon sequencing was used to profile the bacteria associated with Anopheles gambiae sensu lato and identify potential symbionts. The polymerase chain reaction (PCR) with specific primers were subsequently used to monitor symbiont prevalence in field populations, as well as symbiont transmission patterns. Results: We report the discovery of the bacterial symbiont, Spiroplasma, in Anopheles gambiae in Kenya. We determine that geographically dispersed Anopheles gambiae populations in Kenya are infected with Spiroplasma at low prevalence levels. Molecular phylogenetics indicates that this Anopheles gambiae associated Spiroplasma is a member of the insolitum clade. We demonstrate that this symbiont is stably maternally transmitted across at least two generations and does not significantly affect the fecundity or egg to adult survival of its host. Conclusions: In diverse insect species, Spiroplasma has been found to render their host resistant to infection by pathogens. The identification of a maternally transmitted strain of Spiroplasma in Anopheles gambiae may therefore open new lines of investigation for the development of symbiont-based strategies for blocking malaria transmission

Do riparian forest fragments provide ecosystem services or disservices in surrounding oil palm plantations?

Agricultural expansion across tropical regions is causing declines in biodiversity and altering ecological processes. However, in some tropical agricultural systems, conserving natural habitat can simultaneously protect threatened species and support important ecosystem services. Oil palm cultivation is expanding rapidly throughout the tropics but the extent to which non-crop habitat supports biodiversity and ecosystem services in these landscapes is poorly documented. We investigated whether riparian forest fragments (riparian reserves) provide a pest control service or increase pest activity (disservice) within oil palm dominated landscapes in Sabah, Malaysian Borneo. We assessed the activity of potential predators of pest herbivores using plasticine caterpillar mimics and quantified herbivory rates on oil palm fronds in areas with and without riparian reserves. We also manipulated the shape and colour of the mimics to assess the extent to which artificial pest mimics reflect a predatory response. The presence of riparian reserves increased the attack rate on mimics by arthropods, but not by birds. Our methodological study suggested attacks on artificial pest mimics provide a better indication of predatory activity for birds than for arthropod predators. Herbivory rates were also not significantly affected by the presence of a riparian reserve, but we found some evidence that herbivory rates may decrease as the size of riparian reserves increases. Overall, we conclude that riparian forest fragments of 30 – 50 m width on each side of the river are unlikely to provide a pest control service. Nevertheless, our results provide evidence that these riparian buffer strips do not increase the density of defoliating pests, which should reassure managers concerned about possible negative consequences of preserving riparian buffers

Mathematical modelling of the interactive dynamics of wild and <i>Microsporidia MB</i>-infected mosquitoes

A recent discovery highlighted that mosquitoes infected with Microsporidia MB are unable to transmit the Plasmodium to humans. Microsporidia MB is a symbiont transmitted vertically and horizontally in the mosquito population, and these transmission routes are known to favor the persistence of the parasite in the mosquito population. Despite the dual transmission, data from field experiments reveal a low prevalence of MB-infected mosquitoes in nature. This study proposes a compartmental model to understand the prevalence of MB-infected mosquitoes. The dynamic of the model is obtained through the computation of the basic reproduction number and the analysis of the stability of the MB-free and coexistence equilibria. The model shows that, in spite of the high vertical transmission efficiency of Microsporidia MB, there can still be a low prevalence of MB-infected mosquitoes. Numerical analysis of the model shows that male-to-female horizontal transmission contributes more than female-to-male horizontal transmission to the spread of MB-infected mosquitoes. Moreover, the female-to-male horizontal transmission contributes to the spread of the symbiont only if there are multiple mating occurrences for male mosquitoes. Furthermore, when fixing the efficiencies of vertical transmission, the parameters having the greater influence on the ratio of MB-positive to wild mosquitoes are identified. In addition, by assuming a similar impact of the temperature on wild and MB-infected mosquitoes, our model shows the seasonal fluctuation of MB-infected mosquitoes. This study serves as a reference for further studies, on the release strategies of MB-infected mosquitoes, to avoid overestimating the MB-infection spread